Phytoactinopolyspora halotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Jiangellales; Jiangellaceae; Phytoactinopolyspora

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6265 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L9SKN1|A0A6L9SKN1_9ACTN DoxX family protein OS=Phytoactinopolyspora halotolerans OX=1981512 GN=G1H10_31360 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84FDD4 pKa = 3.34RR5 pKa = 11.84HH6 pKa = 5.79SGRR9 pKa = 11.84HH10 pKa = 4.66RR11 pKa = 11.84LAGASALLAATGLMAGGLIATVATSAHH38 pKa = 6.12AAVACEE44 pKa = 3.86VDD46 pKa = 3.73YY47 pKa = 11.48SANTWTDD54 pKa = 3.35GASSGGFTASVTINNLGDD72 pKa = 3.9PVEE75 pKa = 4.45GWALEE80 pKa = 3.97FEE82 pKa = 4.89LPDD85 pKa = 3.9GQSFTQGWSANWDD98 pKa = 3.44ADD100 pKa = 3.73GTSITAEE107 pKa = 3.71NLAWNGTLGTGASTSVGFNGSWTGSYY133 pKa = 9.77TDD135 pKa = 4.34PSAFTLNGVACTGDD149 pKa = 3.67PGNGGDD155 pKa = 4.17NLPPTVSVTSPSHH168 pKa = 5.01GAEE171 pKa = 3.95YY172 pKa = 10.83DD173 pKa = 3.36EE174 pKa = 5.27GATVPLAATASDD186 pKa = 3.96PDD188 pKa = 3.74GTIDD192 pKa = 3.59RR193 pKa = 11.84VEE195 pKa = 4.05FLVDD199 pKa = 3.31GDD201 pKa = 4.36VVATDD206 pKa = 3.7ASAPYY211 pKa = 9.02EE212 pKa = 4.14ATVSGLDD219 pKa = 3.72TGSHH223 pKa = 4.98TASAVAYY230 pKa = 10.15DD231 pKa = 4.7DD232 pKa = 4.89GDD234 pKa = 4.32PEE236 pKa = 5.27LSATDD241 pKa = 3.56SASFTIGDD249 pKa = 4.6DD250 pKa = 4.1GEE252 pKa = 4.43EE253 pKa = 4.45PPPDD257 pKa = 3.8SDD259 pKa = 4.2NPYY262 pKa = 10.62AGADD266 pKa = 3.57VYY268 pKa = 11.68VNPDD272 pKa = 2.6WAGQVLDD279 pKa = 4.51EE280 pKa = 4.77ASSTGGALGAAMEE293 pKa = 4.49EE294 pKa = 4.69VATYY298 pKa = 7.49PTFVWMDD305 pKa = 4.32RR306 pKa = 11.84IGAITDD312 pKa = 3.33GTGLVGHH319 pKa = 7.63LDD321 pKa = 3.4NAVAQDD327 pKa = 3.71QANGATPTVFQVVIYY342 pKa = 9.25NLPNRR347 pKa = 11.84DD348 pKa = 3.57CAASASNGEE357 pKa = 4.29LLISEE362 pKa = 4.92NGFQRR367 pKa = 11.84YY368 pKa = 8.29RR369 pKa = 11.84EE370 pKa = 4.1EE371 pKa = 4.6YY372 pKa = 8.85IDD374 pKa = 5.06PIAEE378 pKa = 3.75ILSRR382 pKa = 11.84PEE384 pKa = 3.74YY385 pKa = 10.28SDD387 pKa = 4.89LRR389 pKa = 11.84IATVIEE395 pKa = 4.32VDD397 pKa = 3.7SLPNLVTNSDD407 pKa = 4.31DD408 pKa = 4.25PDD410 pKa = 3.86CQEE413 pKa = 3.79AAGDD417 pKa = 3.42GGYY420 pKa = 10.03RR421 pKa = 11.84DD422 pKa = 4.56GIRR425 pKa = 11.84YY426 pKa = 8.89ALNEE430 pKa = 3.89LSAIPNVYY438 pKa = 10.38NYY440 pKa = 10.97LDD442 pKa = 3.68IAHH445 pKa = 6.58SGWLGWDD452 pKa = 3.66SNFGPAVDD460 pKa = 5.74LYY462 pKa = 10.65VQTLTSSDD470 pKa = 3.9FYY472 pKa = 11.69GPAPGFDD479 pKa = 3.28SVAGFVTNSANYY491 pKa = 8.73TPIEE495 pKa = 4.28EE496 pKa = 5.16PYY498 pKa = 11.0LPDD501 pKa = 5.34PNLQPGGGGNPIRR514 pKa = 11.84SSDD517 pKa = 3.54FYY519 pKa = 11.24EE520 pKa = 3.75WNPYY524 pKa = 9.5FDD526 pKa = 5.08EE527 pKa = 6.37LDD529 pKa = 3.38FTTDD533 pKa = 2.31IRR535 pKa = 11.84EE536 pKa = 4.16AFISRR541 pKa = 11.84GMPDD545 pKa = 4.8DD546 pKa = 3.62IGMLIDD552 pKa = 3.56TGRR555 pKa = 11.84NGWGGPDD562 pKa = 3.37RR563 pKa = 11.84PSGVSDD569 pKa = 3.17ATDD572 pKa = 2.85VNTYY576 pKa = 9.92VDD578 pKa = 3.68EE579 pKa = 4.51SRR581 pKa = 11.84VDD583 pKa = 3.59RR584 pKa = 11.84RR585 pKa = 11.84PHH587 pKa = 6.03RR588 pKa = 11.84GGWCNQAGAGIGEE601 pKa = 4.74RR602 pKa = 11.84PTVSPGPGIDD612 pKa = 2.88AYY614 pKa = 11.43VWVKK618 pKa = 10.58PPGEE622 pKa = 4.1SDD624 pKa = 3.68GVSDD628 pKa = 5.66PDD630 pKa = 5.17FEE632 pKa = 6.08PDD634 pKa = 3.95PNDD637 pKa = 3.56PNKK640 pKa = 10.75KK641 pKa = 9.17HH642 pKa = 7.13DD643 pKa = 3.9AMCNPEE649 pKa = 4.19GQSTYY654 pKa = 11.41DD655 pKa = 3.25PDD657 pKa = 4.15YY658 pKa = 8.78PTNALPNAPHH668 pKa = 7.03AGRR671 pKa = 11.84WFPEE675 pKa = 3.73QFAMLVEE682 pKa = 4.11NAYY685 pKa = 9.53PAFF688 pKa = 4.42

Molecular weight: 72.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L9SDU8|A0A6L9SDU8_9ACTN Peptidoglycan DD-metalloendopeptidase family protein OS=Phytoactinopolyspora halotolerans OX=1981512 GN=G1H10_24395 PE=4 SV=1
MM1 pKa = 7.49ARR3 pKa = 11.84RR4 pKa = 11.84VLGAGWKK11 pKa = 9.42LLARR15 pKa = 11.84RR16 pKa = 11.84VLRR19 pKa = 11.84ARR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84LLAGRR28 pKa = 11.84EE29 pKa = 3.61RR30 pKa = 11.84LLRR33 pKa = 11.84LRR35 pKa = 11.84MRR37 pKa = 11.84KK38 pKa = 6.35TRR40 pKa = 11.84RR41 pKa = 11.84WTVLRR46 pKa = 11.84IPSRR50 pKa = 11.84TVGAGVGVRR59 pKa = 11.84LRR61 pKa = 11.84SGRR64 pKa = 11.84IRR66 pKa = 11.84RR67 pKa = 11.84LFRR70 pKa = 11.84TVGLAGGVWLLRR82 pKa = 11.84RR83 pKa = 11.84LAGG86 pKa = 3.63

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6265

0

6265

2132900

33

3373

340.4

36.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.701 ± 0.041

0.69 ± 0.009

6.906 ± 0.028

5.986 ± 0.027

2.878 ± 0.017

9.083 ± 0.028

2.29 ± 0.013

3.851 ± 0.02

1.52 ± 0.018

9.799 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.927 ± 0.013

1.912 ± 0.017

5.655 ± 0.02

2.807 ± 0.015

7.916 ± 0.033

5.38 ± 0.018

6.004 ± 0.023

8.914 ± 0.028

1.601 ± 0.016

2.178 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski