Bacillus virus BMBtp2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lwoffvirus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4LPB5|K4LPB5_9CAUD Uncharacterized protein OS=Bacillus virus BMBtp2 OX=1236573 GN=ISGA_34 PE=4 SV=1
MM1 pKa = 7.0ITYY4 pKa = 10.77LLGEE8 pKa = 4.53FIYY11 pKa = 10.79YY12 pKa = 10.07LVVSTAVITPVVAGGVILLEE32 pKa = 4.2KK33 pKa = 11.05AEE35 pKa = 4.57GIINNEE41 pKa = 4.11SKK43 pKa = 11.26QNMDD47 pKa = 3.31EE48 pKa = 4.03

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4LRV3|K4LRV3_9CAUD Uncharacterized protein OS=Bacillus virus BMBtp2 OX=1236573 GN=ISGA_31 PE=4 SV=1
MM1 pKa = 7.67GEE3 pKa = 4.39FISRR7 pKa = 11.84RR8 pKa = 11.84AEE10 pKa = 3.97CEE12 pKa = 3.38RR13 pKa = 11.84RR14 pKa = 11.84TAQLQKK20 pKa = 10.75VALQRR25 pKa = 11.84AIKK28 pKa = 10.11HH29 pKa = 5.83IEE31 pKa = 3.83AQAKK35 pKa = 9.48INTRR39 pKa = 11.84KK40 pKa = 10.14KK41 pKa = 9.89SGATARR47 pKa = 11.84TITSKK52 pKa = 9.37VTGEE56 pKa = 4.21GQNLQAIVGGNDD68 pKa = 3.16DD69 pKa = 3.72NLIYY73 pKa = 10.85EE74 pKa = 4.33EE75 pKa = 4.97FGTGIYY81 pKa = 9.55SEE83 pKa = 4.34KK84 pKa = 10.58NGRR87 pKa = 11.84KK88 pKa = 7.53TPWKK92 pKa = 10.44YY93 pKa = 10.74KK94 pKa = 10.84DD95 pKa = 3.06KK96 pKa = 8.91TTGKK100 pKa = 9.51WYY102 pKa = 8.25VTRR105 pKa = 11.84GKK107 pKa = 10.51KK108 pKa = 7.89GTRR111 pKa = 11.84AMRR114 pKa = 11.84KK115 pKa = 9.42AGEE118 pKa = 4.12SSKK121 pKa = 11.21GQVKK125 pKa = 9.94QIIAQTMKK133 pKa = 10.99GGMGKK138 pKa = 9.96

Molecular weight: 15.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

10649

45

1786

200.9

22.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.625 ± 0.555

0.864 ± 0.169

5.756 ± 0.301

8.461 ± 0.39

3.662 ± 0.16

7.005 ± 0.536

1.634 ± 0.189

6.799 ± 0.22

8.883 ± 0.416

7.137 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.484 ± 0.22

5.841 ± 0.261

2.761 ± 0.232

3.935 ± 0.262

4.958 ± 0.371

5.616 ± 0.26

6.132 ± 0.359

6.47 ± 0.344

1.305 ± 0.138

3.672 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski