Amino acid dipepetide frequency for Bacillus virus BMBtp2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.376AlaCys: 0.376 ± 0.217
3.287AlaAsp: 3.287 ± 0.586
4.696AlaGlu: 4.696 ± 0.677
2.254AlaPhe: 2.254 ± 0.419
4.696AlaGly: 4.696 ± 1.049
1.127AlaHis: 1.127 ± 0.312
5.165AlaIle: 5.165 ± 0.729
5.071AlaLys: 5.071 ± 0.812
4.696AlaLeu: 4.696 ± 0.978
2.066AlaMet: 2.066 ± 0.56
2.348AlaAsn: 2.348 ± 0.422
1.597AlaPro: 1.597 ± 0.455
2.254AlaGln: 2.254 ± 0.52
2.817AlaArg: 2.817 ± 0.501
3.944AlaSer: 3.944 ± 0.883
4.226AlaThr: 4.226 ± 0.564
3.757AlaVal: 3.757 ± 0.848
0.845AlaTrp: 0.845 ± 0.343
1.033AlaTyr: 1.033 ± 0.351
0.0AlaXaa: 0.0 ± 0.0
Cys
0.094CysAla: 0.094 ± 0.085
0.094CysCys: 0.094 ± 0.1
0.751CysAsp: 0.751 ± 0.341
0.845CysGlu: 0.845 ± 0.354
0.094CysPhe: 0.094 ± 0.104
0.845CysGly: 0.845 ± 0.372
0.094CysHis: 0.094 ± 0.082
0.563CysIle: 0.563 ± 0.242
1.033CysLys: 1.033 ± 0.475
0.47CysLeu: 0.47 ± 0.164
0.282CysMet: 0.282 ± 0.21
0.563CysAsn: 0.563 ± 0.227
0.751CysPro: 0.751 ± 0.29
0.188CysGln: 0.188 ± 0.101
0.282CysArg: 0.282 ± 0.166
0.282CysSer: 0.282 ± 0.18
0.282CysThr: 0.282 ± 0.144
0.282CysVal: 0.282 ± 0.161
0.376CysTrp: 0.376 ± 0.169
0.47CysTyr: 0.47 ± 0.175
0.0CysXaa: 0.0 ± 0.0
Asp
4.32AspAla: 4.32 ± 0.641
0.47AspCys: 0.47 ± 0.427
2.348AspAsp: 2.348 ± 0.614
3.944AspGlu: 3.944 ± 0.781
2.442AspPhe: 2.442 ± 0.549
5.165AspGly: 5.165 ± 0.563
1.409AspHis: 1.409 ± 0.517
4.79AspIle: 4.79 ± 0.677
4.602AspLys: 4.602 ± 0.526
4.508AspLeu: 4.508 ± 0.785
1.878AspMet: 1.878 ± 0.432
2.911AspAsn: 2.911 ± 0.65
1.972AspPro: 1.972 ± 0.407
2.254AspGln: 2.254 ± 0.452
2.536AspArg: 2.536 ± 0.549
2.442AspSer: 2.442 ± 0.521
3.099AspThr: 3.099 ± 0.488
3.85AspVal: 3.85 ± 0.538
0.563AspTrp: 0.563 ± 0.193
2.066AspTyr: 2.066 ± 0.366
0.0AspXaa: 0.0 ± 0.0
Glu
5.917GluAla: 5.917 ± 0.877
1.69GluCys: 1.69 ± 0.54
3.287GluAsp: 3.287 ± 0.61
6.011GluGlu: 6.011 ± 0.974
3.381GluPhe: 3.381 ± 0.46
4.884GluGly: 4.884 ± 0.796
1.127GluHis: 1.127 ± 0.319
6.386GluIle: 6.386 ± 1.015
7.795GluLys: 7.795 ± 0.808
6.574GluLeu: 6.574 ± 0.704
4.038GluMet: 4.038 ± 0.568
4.414GluAsn: 4.414 ± 0.54
1.315GluPro: 1.315 ± 0.374
3.381GluGln: 3.381 ± 0.443
4.79GluArg: 4.79 ± 0.903
4.32GluSer: 4.32 ± 0.504
5.447GluThr: 5.447 ± 0.642
5.823GluVal: 5.823 ± 0.849
1.315GluTrp: 1.315 ± 0.472
2.63GluTyr: 2.63 ± 0.66
0.0GluXaa: 0.0 ± 0.0
Phe
2.16PheAla: 2.16 ± 0.347
0.094PheCys: 0.094 ± 0.085
2.348PheAsp: 2.348 ± 0.484
2.724PheGlu: 2.724 ± 0.5
0.657PhePhe: 0.657 ± 0.253
3.005PheGly: 3.005 ± 0.608
0.751PheHis: 0.751 ± 0.276
2.63PheIle: 2.63 ± 0.432
2.536PheLys: 2.536 ± 0.428
1.69PheLeu: 1.69 ± 0.457
1.221PheMet: 1.221 ± 0.338
2.63PheAsn: 2.63 ± 0.53
0.751PhePro: 0.751 ± 0.265
1.784PheGln: 1.784 ± 0.609
1.784PheArg: 1.784 ± 0.546
2.817PheSer: 2.817 ± 0.428
3.005PheThr: 3.005 ± 0.547
1.784PheVal: 1.784 ± 0.376
0.282PheTrp: 0.282 ± 0.13
1.972PheTyr: 1.972 ± 0.388
0.0PheXaa: 0.0 ± 0.0
Gly
2.536GlyAla: 2.536 ± 0.666
0.563GlyCys: 0.563 ± 0.286
3.381GlyAsp: 3.381 ± 0.529
6.011GlyGlu: 6.011 ± 0.927
3.287GlyPhe: 3.287 ± 0.66
4.038GlyGly: 4.038 ± 0.966
0.939GlyHis: 0.939 ± 0.246
4.884GlyIle: 4.884 ± 0.541
7.325GlyLys: 7.325 ± 0.68
4.226GlyLeu: 4.226 ± 0.991
2.348GlyMet: 2.348 ± 0.519
4.884GlyAsn: 4.884 ± 0.536
1.409GlyPro: 1.409 ± 0.424
2.066GlyGln: 2.066 ± 0.549
3.287GlyArg: 3.287 ± 0.63
4.226GlySer: 4.226 ± 0.648
4.602GlyThr: 4.602 ± 0.945
5.165GlyVal: 5.165 ± 0.62
1.127GlyTrp: 1.127 ± 0.325
3.757GlyTyr: 3.757 ± 0.555
0.0GlyXaa: 0.0 ± 0.0
His
1.033HisAla: 1.033 ± 0.316
0.188HisCys: 0.188 ± 0.11
1.503HisAsp: 1.503 ± 0.401
0.939HisGlu: 0.939 ± 0.284
0.939HisPhe: 0.939 ± 0.264
1.315HisGly: 1.315 ± 0.36
0.376HisHis: 0.376 ± 0.157
1.409HisIle: 1.409 ± 0.371
1.127HisLys: 1.127 ± 0.343
1.69HisLeu: 1.69 ± 0.394
0.376HisMet: 0.376 ± 0.179
1.127HisAsn: 1.127 ± 0.355
0.094HisPro: 0.094 ± 0.085
0.47HisGln: 0.47 ± 0.169
0.845HisArg: 0.845 ± 0.286
0.657HisSer: 0.657 ± 0.22
0.47HisThr: 0.47 ± 0.168
0.939HisVal: 0.939 ± 0.259
0.188HisTrp: 0.188 ± 0.14
0.657HisTyr: 0.657 ± 0.283
0.0HisXaa: 0.0 ± 0.0
Ile
4.32IleAla: 4.32 ± 0.633
0.47IleCys: 0.47 ± 0.163
4.696IleAsp: 4.696 ± 0.826
6.386IleGlu: 6.386 ± 0.688
1.127IlePhe: 1.127 ± 0.341
3.569IleGly: 3.569 ± 0.904
1.315IleHis: 1.315 ± 0.256
2.63IleIle: 2.63 ± 0.502
6.386IleLys: 6.386 ± 0.885
3.193IleLeu: 3.193 ± 0.563
1.972IleMet: 1.972 ± 0.458
4.977IleAsn: 4.977 ± 0.753
2.066IlePro: 2.066 ± 0.522
2.442IleGln: 2.442 ± 0.569
3.757IleArg: 3.757 ± 0.595
5.823IleSer: 5.823 ± 0.934
4.602IleThr: 4.602 ± 0.582
5.259IleVal: 5.259 ± 0.572
0.47IleTrp: 0.47 ± 0.189
2.536IleTyr: 2.536 ± 0.657
0.0IleXaa: 0.0 ± 0.0
Lys
4.977LysAla: 4.977 ± 0.636
0.563LysCys: 0.563 ± 0.262
5.071LysAsp: 5.071 ± 0.645
8.922LysGlu: 8.922 ± 0.861
2.254LysPhe: 2.254 ± 0.418
6.668LysGly: 6.668 ± 0.677
1.878LysHis: 1.878 ± 0.463
4.884LysIle: 4.884 ± 0.548
7.889LysLys: 7.889 ± 1.228
6.011LysLeu: 6.011 ± 0.817
3.287LysMet: 3.287 ± 0.52
3.944LysAsn: 3.944 ± 0.457
2.817LysPro: 2.817 ± 0.495
3.85LysGln: 3.85 ± 0.516
5.259LysArg: 5.259 ± 0.811
4.884LysSer: 4.884 ± 0.632
4.977LysThr: 4.977 ± 0.939
6.198LysVal: 6.198 ± 0.677
1.127LysTrp: 1.127 ± 0.325
3.381LysTyr: 3.381 ± 0.672
0.0LysXaa: 0.0 ± 0.0
Leu
3.569LeuAla: 3.569 ± 0.895
0.563LeuCys: 0.563 ± 0.228
5.447LeuAsp: 5.447 ± 0.641
6.95LeuGlu: 6.95 ± 1.214
2.16LeuPhe: 2.16 ± 0.44
4.414LeuGly: 4.414 ± 1.02
1.221LeuHis: 1.221 ± 0.382
3.944LeuIle: 3.944 ± 0.555
6.292LeuLys: 6.292 ± 0.683
6.104LeuLeu: 6.104 ± 0.719
2.442LeuMet: 2.442 ± 0.404
3.757LeuAsn: 3.757 ± 0.476
1.784LeuPro: 1.784 ± 0.56
3.099LeuGln: 3.099 ± 0.465
3.099LeuArg: 3.099 ± 0.661
4.977LeuSer: 4.977 ± 0.784
3.569LeuThr: 3.569 ± 0.506
5.165LeuVal: 5.165 ± 0.52
0.845LeuTrp: 0.845 ± 0.287
1.972LeuTyr: 1.972 ± 0.329
0.0LeuXaa: 0.0 ± 0.0
Met
1.69MetAla: 1.69 ± 0.666
0.188MetCys: 0.188 ± 0.128
1.69MetAsp: 1.69 ± 0.348
2.724MetGlu: 2.724 ± 0.486
1.597MetPhe: 1.597 ± 0.462
1.878MetGly: 1.878 ± 0.353
0.376MetHis: 0.376 ± 0.186
1.878MetIle: 1.878 ± 0.551
4.32MetLys: 4.32 ± 0.553
1.69MetLeu: 1.69 ± 0.367
0.751MetMet: 0.751 ± 0.348
2.817MetAsn: 2.817 ± 0.458
1.033MetPro: 1.033 ± 0.314
1.221MetGln: 1.221 ± 0.334
2.911MetArg: 2.911 ± 0.425
2.724MetSer: 2.724 ± 0.73
2.442MetThr: 2.442 ± 0.4
1.409MetVal: 1.409 ± 0.334
0.188MetTrp: 0.188 ± 0.143
1.315MetTyr: 1.315 ± 0.318
0.0MetXaa: 0.0 ± 0.0
Asn
3.663AsnAla: 3.663 ± 0.507
0.094AsnCys: 0.094 ± 0.1
4.226AsnAsp: 4.226 ± 0.83
4.79AsnGlu: 4.79 ± 0.564
1.221AsnPhe: 1.221 ± 0.366
4.79AsnGly: 4.79 ± 0.905
0.845AsnHis: 0.845 ± 0.296
2.817AsnIle: 2.817 ± 0.585
6.104AsnLys: 6.104 ± 0.823
3.944AsnLeu: 3.944 ± 0.647
2.442AsnMet: 2.442 ± 0.442
4.508AsnAsn: 4.508 ± 0.882
2.442AsnPro: 2.442 ± 0.505
2.066AsnGln: 2.066 ± 0.371
2.536AsnArg: 2.536 ± 0.646
2.724AsnSer: 2.724 ± 0.454
3.944AsnThr: 3.944 ± 0.516
2.817AsnVal: 2.817 ± 0.472
0.751AsnTrp: 0.751 ± 0.243
1.69AsnTyr: 1.69 ± 0.386
0.0AsnXaa: 0.0 ± 0.0
Pro
1.69ProAla: 1.69 ± 0.281
0.094ProCys: 0.094 ± 0.075
1.127ProAsp: 1.127 ± 0.332
2.536ProGlu: 2.536 ± 0.542
1.503ProPhe: 1.503 ± 0.41
2.254ProGly: 2.254 ± 0.601
0.282ProHis: 0.282 ± 0.146
1.503ProIle: 1.503 ± 0.393
2.63ProLys: 2.63 ± 0.567
1.878ProLeu: 1.878 ± 0.387
0.657ProMet: 0.657 ± 0.216
1.784ProAsn: 1.784 ± 0.361
0.939ProPro: 0.939 ± 0.204
0.845ProGln: 0.845 ± 0.272
1.127ProArg: 1.127 ± 0.574
1.409ProSer: 1.409 ± 0.347
1.597ProThr: 1.597 ± 0.341
2.348ProVal: 2.348 ± 0.477
0.282ProTrp: 0.282 ± 0.174
1.127ProTyr: 1.127 ± 0.327
0.0ProXaa: 0.0 ± 0.0
Gln
2.442GlnAla: 2.442 ± 0.559
0.282GlnCys: 0.282 ± 0.172
2.066GlnAsp: 2.066 ± 0.407
3.569GlnGlu: 3.569 ± 0.627
1.409GlnPhe: 1.409 ± 0.29
2.63GlnGly: 2.63 ± 0.562
0.563GlnHis: 0.563 ± 0.267
2.63GlnIle: 2.63 ± 0.37
3.099GlnLys: 3.099 ± 0.472
3.944GlnLeu: 3.944 ± 0.674
1.127GlnMet: 1.127 ± 0.379
1.409GlnAsn: 1.409 ± 0.463
1.127GlnPro: 1.127 ± 0.404
2.63GlnGln: 2.63 ± 0.733
1.784GlnArg: 1.784 ± 0.416
2.254GlnSer: 2.254 ± 0.48
1.972GlnThr: 1.972 ± 0.376
2.348GlnVal: 2.348 ± 0.552
0.563GlnTrp: 0.563 ± 0.252
1.503GlnTyr: 1.503 ± 0.379
0.0GlnXaa: 0.0 ± 0.0
Arg
2.442ArgAla: 2.442 ± 0.435
0.751ArgCys: 0.751 ± 0.298
2.348ArgAsp: 2.348 ± 0.413
4.038ArgGlu: 4.038 ± 0.745
1.878ArgPhe: 1.878 ± 0.471
2.817ArgGly: 2.817 ± 0.604
0.563ArgHis: 0.563 ± 0.21
3.757ArgIle: 3.757 ± 0.686
4.414ArgLys: 4.414 ± 0.835
4.414ArgLeu: 4.414 ± 0.652
2.254ArgMet: 2.254 ± 0.433
2.911ArgAsn: 2.911 ± 0.612
0.845ArgPro: 0.845 ± 0.352
2.254ArgGln: 2.254 ± 0.493
2.536ArgArg: 2.536 ± 0.532
2.254ArgSer: 2.254 ± 0.454
2.724ArgThr: 2.724 ± 0.634
3.287ArgVal: 3.287 ± 0.56
0.657ArgTrp: 0.657 ± 0.268
2.442ArgTyr: 2.442 ± 0.479
0.0ArgXaa: 0.0 ± 0.0
Ser
3.475SerAla: 3.475 ± 0.996
0.657SerCys: 0.657 ± 0.233
2.724SerAsp: 2.724 ± 0.641
5.071SerGlu: 5.071 ± 0.731
2.724SerPhe: 2.724 ± 0.5
4.414SerGly: 4.414 ± 0.574
0.563SerHis: 0.563 ± 0.332
4.132SerIle: 4.132 ± 0.618
4.414SerLys: 4.414 ± 0.514
4.414SerLeu: 4.414 ± 0.694
2.066SerMet: 2.066 ± 0.47
3.757SerAsn: 3.757 ± 0.732
1.315SerPro: 1.315 ± 0.272
2.254SerGln: 2.254 ± 0.385
1.69SerArg: 1.69 ± 0.532
2.442SerSer: 2.442 ± 0.436
3.005SerThr: 3.005 ± 0.495
4.132SerVal: 4.132 ± 0.611
0.751SerTrp: 0.751 ± 0.222
2.16SerTyr: 2.16 ± 0.487
0.0SerXaa: 0.0 ± 0.0
Thr
4.79ThrAla: 4.79 ± 0.664
0.376ThrCys: 0.376 ± 0.235
3.85ThrAsp: 3.85 ± 0.685
4.414ThrGlu: 4.414 ± 0.766
3.099ThrPhe: 3.099 ± 0.528
5.165ThrGly: 5.165 ± 0.613
0.657ThrHis: 0.657 ± 0.213
5.259ThrIle: 5.259 ± 0.41
5.165ThrLys: 5.165 ± 0.574
4.602ThrLeu: 4.602 ± 0.567
1.972ThrMet: 1.972 ± 0.362
2.724ThrAsn: 2.724 ± 0.56
2.724ThrPro: 2.724 ± 0.486
2.16ThrGln: 2.16 ± 0.607
2.536ThrArg: 2.536 ± 0.541
2.536ThrSer: 2.536 ± 0.427
2.536ThrThr: 2.536 ± 0.502
3.85ThrVal: 3.85 ± 0.417
0.563ThrTrp: 0.563 ± 0.208
2.348ThrTyr: 2.348 ± 0.414
0.0ThrXaa: 0.0 ± 0.0
Val
4.32ValAla: 4.32 ± 0.84
0.376ValCys: 0.376 ± 0.2
3.569ValAsp: 3.569 ± 0.466
6.104ValGlu: 6.104 ± 0.555
2.536ValPhe: 2.536 ± 0.588
4.038ValGly: 4.038 ± 0.84
1.221ValHis: 1.221 ± 0.304
5.635ValIle: 5.635 ± 0.903
5.635ValLys: 5.635 ± 0.501
3.475ValLeu: 3.475 ± 0.509
1.784ValMet: 1.784 ± 0.361
3.85ValAsn: 3.85 ± 0.562
1.315ValPro: 1.315 ± 0.258
2.348ValGln: 2.348 ± 0.483
3.099ValArg: 3.099 ± 0.514
2.911ValSer: 2.911 ± 0.428
6.011ValThr: 6.011 ± 0.745
3.85ValVal: 3.85 ± 0.699
1.033ValTrp: 1.033 ± 0.295
1.597ValTyr: 1.597 ± 0.366
0.0ValXaa: 0.0 ± 0.0
Trp
0.563TrpAla: 0.563 ± 0.287
0.282TrpCys: 0.282 ± 0.244
1.033TrpAsp: 1.033 ± 0.289
0.939TrpGlu: 0.939 ± 0.267
0.563TrpPhe: 0.563 ± 0.207
0.939TrpGly: 0.939 ± 0.252
0.094TrpHis: 0.094 ± 0.084
1.033TrpIle: 1.033 ± 0.325
0.845TrpLys: 0.845 ± 0.263
1.503TrpLeu: 1.503 ± 0.359
0.282TrpMet: 0.282 ± 0.149
1.033TrpAsn: 1.033 ± 0.318
0.188TrpPro: 0.188 ± 0.145
0.751TrpGln: 0.751 ± 0.26
0.282TrpArg: 0.282 ± 0.164
0.845TrpSer: 0.845 ± 0.254
0.563TrpThr: 0.563 ± 0.307
0.563TrpVal: 0.563 ± 0.19
0.188TrpTrp: 0.188 ± 0.15
0.563TrpTyr: 0.563 ± 0.279
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.254TyrAla: 2.254 ± 0.468
0.47TyrCys: 0.47 ± 0.214
2.817TyrAsp: 2.817 ± 0.45
3.005TyrGlu: 3.005 ± 0.607
1.503TyrPhe: 1.503 ± 0.356
2.536TyrGly: 2.536 ± 0.463
0.939TyrHis: 0.939 ± 0.256
2.066TyrIle: 2.066 ± 0.434
2.16TyrLys: 2.16 ± 0.616
2.348TyrLeu: 2.348 ± 0.653
1.503TyrMet: 1.503 ± 0.413
2.066TyrAsn: 2.066 ± 0.395
1.127TyrPro: 1.127 ± 0.315
1.033TyrGln: 1.033 ± 0.263
2.63TyrArg: 2.63 ± 0.532
1.69TyrSer: 1.69 ± 0.459
2.254TyrThr: 2.254 ± 0.439
1.878TyrVal: 1.878 ± 0.368
0.939TyrTrp: 0.939 ± 0.322
1.503TyrTyr: 1.503 ± 0.338
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (10649 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski