Ictidomys tridecemlineatus (Thirteen-lined ground squirrel) (Spermophilus tridecemlineatus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria;

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25333 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A287DGJ4|A0A287DGJ4_ICTTR Isoform of I3M2P5 MAX dimerization protein MLX OS=Ictidomys tridecemlineatus OX=43179 GN=MLX PE=4 SV=1
AA1 pKa = 8.25NEE3 pKa = 4.27VTDD6 pKa = 3.78SAYY9 pKa = 10.03MGSEE13 pKa = 3.91STYY16 pKa = 10.37SEE18 pKa = 4.83CEE20 pKa = 3.95TFTDD24 pKa = 4.04EE25 pKa = 4.23DD26 pKa = 4.41TSTLVHH32 pKa = 7.13PEE34 pKa = 4.02LQPEE38 pKa = 4.26GDD40 pKa = 3.75ADD42 pKa = 3.95SAGGSAVPSEE52 pKa = 4.82CLDD55 pKa = 3.38AMEE58 pKa = 4.78EE59 pKa = 4.19TDD61 pKa = 5.33HH62 pKa = 7.06GALLLLPGRR71 pKa = 11.84LRR73 pKa = 11.84PRR75 pKa = 11.84SQAVVMVMSSEE86 pKa = 4.12EE87 pKa = 3.85HH88 pKa = 6.23FEE90 pKa = 4.76DD91 pKa = 4.37YY92 pKa = 11.67GEE94 pKa = 4.25GG95 pKa = 3.49

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3N090|I3N090_ICTTR Interleukin-6 OS=Ictidomys tridecemlineatus OX=43179 PE=3 SV=2
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18446

6887

25333

14498878

10

7747

572.3

63.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.82 ± 0.016

2.174 ± 0.015

4.885 ± 0.011

7.122 ± 0.02

3.697 ± 0.01

6.42 ± 0.022

2.575 ± 0.007

4.497 ± 0.014

5.782 ± 0.017

9.998 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.006

3.695 ± 0.01

6.175 ± 0.023

4.784 ± 0.013

5.592 ± 0.013

8.33 ± 0.018

5.308 ± 0.01

6.045 ± 0.012

1.176 ± 0.005

2.71 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski