Amino acid dipepetide frequency for Ictidomys tridecemlineatus (Thirteen-lined ground squirrel) (Spermophilus tridecemlineatus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.496AlaAla: 6.496 ± 0.035	1.377AlaCys: 1.377 ± 0.013	2.878AlaAsp: 2.878 ± 0.017	4.679AlaGlu: 4.679 ± 0.029	2.617AlaPhe: 2.617 ± 0.018	4.482AlaGly: 4.482 ± 0.027	1.554AlaHis: 1.554 ± 0.011	2.886AlaIle: 2.886 ± 0.018	3.395AlaLys: 3.395 ± 0.022	7.183AlaLeu: 7.183 ± 0.032	1.5AlaMet: 1.5 ± 0.012	2.051AlaAsn: 2.051 ± 0.013	4.029AlaPro: 4.029 ± 0.028	3.239AlaGln: 3.239 ± 0.021	3.585AlaArg: 3.585 ± 0.021	5.711AlaSer: 5.711 ± 0.026	3.595AlaThr: 3.595 ± 0.018	4.626AlaVal: 4.626 ± 0.023	0.77AlaTrp: 0.77 ± 0.009	1.544AlaTyr: 1.544 ± 0.012	0.001AlaXaa: 0.001 ± 0.0
Cys	1.254CysAla: 1.254 ± 0.013	0.619CysCys: 0.619 ± 0.01	1.044CysAsp: 1.044 ± 0.012	1.263CysGlu: 1.263 ± 0.013	0.843CysPhe: 0.843 ± 0.009	1.68CysGly: 1.68 ± 0.019	0.668CysHis: 0.668 ± 0.007	0.96CysIle: 0.96 ± 0.011	1.133CysLys: 1.133 ± 0.011	2.146CysLeu: 2.146 ± 0.016	0.417CysMet: 0.417 ± 0.005	0.804CysAsn: 0.804 ± 0.009	1.365CysPro: 1.365 ± 0.019	1.055CysGln: 1.055 ± 0.012	1.268CysArg: 1.268 ± 0.012	1.985CysSer: 1.985 ± 0.017	1.084CysThr: 1.084 ± 0.011	1.287CysVal: 1.287 ± 0.012	0.285CysTrp: 0.285 ± 0.005	0.576CysTyr: 0.576 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	2.825AspAla: 2.825 ± 0.016	1.06AspCys: 1.06 ± 0.012	2.666AspAsp: 2.666 ± 0.018	3.46AspGlu: 3.46 ± 0.021	2.15AspPhe: 2.15 ± 0.014	3.311AspGly: 3.311 ± 0.021	1.162AspHis: 1.162 ± 0.01	2.641AspIle: 2.641 ± 0.017	2.524AspLys: 2.524 ± 0.016	5.076AspLeu: 5.076 ± 0.021	1.132AspMet: 1.132 ± 0.009	1.732AspAsn: 1.732 ± 0.011	2.956AspPro: 2.956 ± 0.017	1.853AspGln: 1.853 ± 0.011	2.481AspArg: 2.481 ± 0.015	4.217AspSer: 4.217 ± 0.024	2.466AspThr: 2.466 ± 0.013	3.008AspVal: 3.008 ± 0.017	0.624AspTrp: 0.624 ± 0.007	1.509AspTyr: 1.509 ± 0.012	0.0AspXaa: 0.0 ± 0.0
Glu	5.167GluAla: 5.167 ± 0.03	1.359GluCys: 1.359 ± 0.019	4.491GluAsp: 4.491 ± 0.02	8.038GluGlu: 8.038 ± 0.053	2.082GluPhe: 2.082 ± 0.013	4.224GluGly: 4.224 ± 0.024	1.492GluHis: 1.492 ± 0.01	3.18GluIle: 3.18 ± 0.018	5.36GluLys: 5.36 ± 0.032	6.487GluLeu: 6.487 ± 0.031	1.665GluMet: 1.665 ± 0.011	3.124GluAsn: 3.124 ± 0.018	3.237GluPro: 3.237 ± 0.021	3.162GluGln: 3.162 ± 0.021	3.959GluArg: 3.959 ± 0.025	4.365GluSer: 4.365 ± 0.021	3.34GluThr: 3.34 ± 0.019	4.197GluVal: 4.197 ± 0.022	0.684GluTrp: 0.684 ± 0.008	1.612GluTyr: 1.612 ± 0.011	0.0GluXaa: 0.0 ± 0.0
Phe	2.014PheAla: 2.014 ± 0.014	0.975PheCys: 0.975 ± 0.01	1.711PheAsp: 1.711 ± 0.011	2.025PheGlu: 2.025 ± 0.015	1.693PhePhe: 1.693 ± 0.016	2.237PheGly: 2.237 ± 0.016	1.044PheHis: 1.044 ± 0.01	1.926PheIle: 1.926 ± 0.015	1.788PheLys: 1.788 ± 0.011	4.226PheLeu: 4.226 ± 0.026	0.793PheMet: 0.793 ± 0.009	1.374PheAsn: 1.374 ± 0.011	2.007PhePro: 2.007 ± 0.013	1.809PheGln: 1.809 ± 0.013	1.957PheArg: 1.957 ± 0.014	3.429PheSer: 3.429 ± 0.018	2.037PheThr: 2.037 ± 0.016	2.192PheVal: 2.192 ± 0.016	0.498PheTrp: 0.498 ± 0.007	1.234PheTyr: 1.234 ± 0.011	0.0PheXaa: 0.0 ± 0.0
Gly	4.318GlyAla: 4.318 ± 0.025	1.26GlyCys: 1.26 ± 0.014	3.165GlyAsp: 3.165 ± 0.02	4.054GlyGlu: 4.054 ± 0.029	2.446GlyPhe: 2.446 ± 0.016	4.915GlyGly: 4.915 ± 0.042	1.699GlyHis: 1.699 ± 0.013	2.782GlyIle: 2.782 ± 0.017	3.715GlyLys: 3.715 ± 0.022	5.921GlyLeu: 5.921 ± 0.027	1.308GlyMet: 1.308 ± 0.012	2.42GlyAsn: 2.42 ± 0.016	4.234GlyPro: 4.234 ± 0.048	2.765GlyGln: 2.765 ± 0.017	3.67GlyArg: 3.67 ± 0.022	5.835GlySer: 5.835 ± 0.033	3.652GlyThr: 3.652 ± 0.021	3.502GlyVal: 3.502 ± 0.019	0.778GlyTrp: 0.778 ± 0.01	1.763GlyTyr: 1.763 ± 0.017	0.0GlyXaa: 0.0 ± 0.0
His	1.302HisAla: 1.302 ± 0.01	0.726HisCys: 0.726 ± 0.01	0.871HisAsp: 0.871 ± 0.008	1.302HisGlu: 1.302 ± 0.011	1.121HisPhe: 1.121 ± 0.009	1.523HisGly: 1.523 ± 0.013	0.891HisHis: 0.891 ± 0.012	1.278HisIle: 1.278 ± 0.011	1.261HisLys: 1.261 ± 0.009	2.934HisLeu: 2.934 ± 0.015	0.602HisMet: 0.602 ± 0.007	0.877HisAsn: 0.877 ± 0.009	1.659HisPro: 1.659 ± 0.013	1.326HisGln: 1.326 ± 0.014	1.602HisArg: 1.602 ± 0.012	2.288HisSer: 2.288 ± 0.017	1.495HisThr: 1.495 ± 0.014	1.5HisVal: 1.5 ± 0.011	0.35HisTrp: 0.35 ± 0.006	0.843HisTyr: 0.843 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	2.613IleAla: 2.613 ± 0.015	1.1IleCys: 1.1 ± 0.009	2.065IleAsp: 2.065 ± 0.013	2.652IleGlu: 2.652 ± 0.017	1.956IlePhe: 1.956 ± 0.017	2.243IleGly: 2.243 ± 0.015	1.339IleHis: 1.339 ± 0.013	2.475IleIle: 2.475 ± 0.019	2.656IleLys: 2.656 ± 0.018	4.737IleLeu: 4.737 ± 0.026	1.04IleMet: 1.04 ± 0.009	1.865IleAsn: 1.865 ± 0.013	2.679IlePro: 2.679 ± 0.016	2.318IleGln: 2.318 ± 0.014	2.411IleArg: 2.411 ± 0.015	3.747IleSer: 3.747 ± 0.019	2.575IleThr: 2.575 ± 0.017	2.543IleVal: 2.543 ± 0.017	0.509IleTrp: 0.509 ± 0.007	1.451IleTyr: 1.451 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	3.951LysAla: 3.951 ± 0.021	1.11LysCys: 1.11 ± 0.012	3.144LysAsp: 3.144 ± 0.018	5.102LysGlu: 5.102 ± 0.03	1.759LysPhe: 1.759 ± 0.011	3.215LysGly: 3.215 ± 0.028	1.401LysHis: 1.401 ± 0.012	2.782LysIle: 2.782 ± 0.019	4.761LysLys: 4.761 ± 0.033	5.18LysLeu: 5.18 ± 0.025	1.453LysMet: 1.453 ± 0.012	2.408LysAsn: 2.408 ± 0.017	3.054LysPro: 3.054 ± 0.021	2.605LysGln: 2.605 ± 0.019	3.286LysArg: 3.286 ± 0.018	3.9LysSer: 3.9 ± 0.022	3.059LysThr: 3.059 ± 0.02	3.468LysVal: 3.468 ± 0.019	0.596LysTrp: 0.596 ± 0.008	1.588LysTyr: 1.588 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	6.887LeuAla: 6.887 ± 0.033	2.196LeuCys: 2.196 ± 0.015	4.816LeuAsp: 4.816 ± 0.018	7.211LeuGlu: 7.211 ± 0.04	3.493LeuPhe: 3.493 ± 0.022	5.962LeuGly: 5.962 ± 0.027	2.776LeuHis: 2.776 ± 0.018	3.976LeuIle: 3.976 ± 0.023	5.801LeuLys: 5.801 ± 0.028	10.931LeuLeu: 10.931 ± 0.048	2.054LeuMet: 2.054 ± 0.014	3.543LeuAsn: 3.543 ± 0.019	5.975LeuPro: 5.975 ± 0.03	5.845LeuGln: 5.845 ± 0.031	6.02LeuArg: 6.02 ± 0.029	8.067LeuSer: 8.067 ± 0.031	5.084LeuThr: 5.084 ± 0.023	5.619LeuVal: 5.619 ± 0.028	1.133LeuTrp: 1.133 ± 0.01	2.588LeuTyr: 2.588 ± 0.015	0.001LeuXaa: 0.001 ± 0.0
Met	1.921MetAla: 1.921 ± 0.014	0.399MetCys: 0.399 ± 0.006	1.279MetAsp: 1.279 ± 0.01	1.889MetGlu: 1.889 ± 0.013	0.74MetPhe: 0.74 ± 0.008	1.304MetGly: 1.304 ± 0.011	0.463MetHis: 0.463 ± 0.006	0.864MetIle: 0.864 ± 0.009	1.446MetLys: 1.446 ± 0.012	2.015MetLeu: 2.015 ± 0.015	0.577MetMet: 0.577 ± 0.008	0.911MetAsn: 0.911 ± 0.01	1.087MetPro: 1.087 ± 0.011	0.937MetGln: 0.937 ± 0.008	1.048MetArg: 1.048 ± 0.009	1.599MetSer: 1.599 ± 0.012	1.126MetThr: 1.126 ± 0.009	1.417MetVal: 1.417 ± 0.011	0.235MetTrp: 0.235 ± 0.005	0.635MetTyr: 0.635 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.047AsnAla: 2.047 ± 0.015	0.84AsnCys: 0.84 ± 0.011	1.54AsnAsp: 1.54 ± 0.013	2.217AsnGlu: 2.217 ± 0.017	1.52AsnPhe: 1.52 ± 0.011	2.5AsnGly: 2.5 ± 0.017	0.963AsnHis: 0.963 ± 0.009	2.147AsnIle: 2.147 ± 0.014	2.245AsnLys: 2.245 ± 0.014	3.809AsnLeu: 3.809 ± 0.019	0.92AsnMet: 0.92 ± 0.009	1.536AsnAsn: 1.536 ± 0.013	2.224AsnPro: 2.224 ± 0.013	1.687AsnGln: 1.687 ± 0.014	1.855AsnArg: 1.855 ± 0.011	3.166AsnSer: 3.166 ± 0.019	1.974AsnThr: 1.974 ± 0.013	2.181AsnVal: 2.181 ± 0.015	0.452AsnTrp: 0.452 ± 0.006	1.127AsnTyr: 1.127 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	4.725ProAla: 4.725 ± 0.03	1.144ProCys: 1.144 ± 0.017	2.77ProAsp: 2.77 ± 0.014	4.47ProGlu: 4.47 ± 0.025	1.941ProPhe: 1.941 ± 0.016	5.237ProGly: 5.237 ± 0.07	1.453ProHis: 1.453 ± 0.011	1.909ProIle: 1.909 ± 0.012	2.78ProLys: 2.78 ± 0.018	5.327ProLeu: 5.327 ± 0.026	1.111ProMet: 1.111 ± 0.01	1.859ProAsn: 1.859 ± 0.013	6.112ProPro: 6.112 ± 0.063	2.839ProGln: 2.839 ± 0.019	3.281ProArg: 3.281 ± 0.019	5.667ProSer: 5.667 ± 0.029	3.126ProThr: 3.126 ± 0.02	3.823ProVal: 3.823 ± 0.021	0.679ProTrp: 0.679 ± 0.008	1.493ProTyr: 1.493 ± 0.014	0.001ProXaa: 0.001 ± 0.0
Gln	3.497GlnAla: 3.497 ± 0.019	0.924GlnCys: 0.924 ± 0.012	2.373GlnAsp: 2.373 ± 0.015	3.905GlnGlu: 3.905 ± 0.024	1.392GlnPhe: 1.392 ± 0.01	2.873GlnGly: 2.873 ± 0.02	1.286GlnHis: 1.286 ± 0.011	2.011GlnIle: 2.011 ± 0.013	2.91GlnLys: 2.91 ± 0.017	4.825GlnLeu: 4.825 ± 0.027	1.125GlnMet: 1.125 ± 0.01	1.878GlnAsn: 1.878 ± 0.013	2.786GlnPro: 2.786 ± 0.02	3.121GlnGln: 3.121 ± 0.037	2.943GlnArg: 2.943 ± 0.02	3.155GlnSer: 3.155 ± 0.019	2.28GlnThr: 2.28 ± 0.013	2.87GlnVal: 2.87 ± 0.018	0.531GlnTrp: 0.531 ± 0.007	1.156GlnTyr: 1.156 ± 0.01	0.0GlnXaa: 0.0 ± 0.0
Arg	3.764ArgAla: 3.764 ± 0.022	1.2ArgCys: 1.2 ± 0.014	2.739ArgAsp: 2.739 ± 0.017	3.948ArgGlu: 3.948 ± 0.024	1.878ArgPhe: 1.878 ± 0.012	3.54ArgGly: 3.54 ± 0.026	1.513ArgHis: 1.513 ± 0.011	2.463ArgIle: 2.463 ± 0.016	3.705ArgLys: 3.705 ± 0.018	5.412ArgLeu: 5.412 ± 0.03	1.184ArgMet: 1.184 ± 0.011	2.14ArgAsn: 2.14 ± 0.012	3.192ArgPro: 3.192 ± 0.018	2.608ArgGln: 2.608 ± 0.016	4.278ArgArg: 4.278 ± 0.026	4.238ArgSer: 4.238 ± 0.032	2.803ArgThr: 2.803 ± 0.016	3.15ArgVal: 3.15 ± 0.016	0.672ArgTrp: 0.672 ± 0.008	1.492ArgTyr: 1.492 ± 0.01	0.001ArgXaa: 0.001 ± 0.0
Ser	5.245SerAla: 5.245 ± 0.022	1.815SerCys: 1.815 ± 0.018	3.918SerAsp: 3.918 ± 0.025	5.164SerGlu: 5.164 ± 0.026	3.059SerPhe: 3.059 ± 0.018	5.635SerGly: 5.635 ± 0.027	2.132SerHis: 2.132 ± 0.016	3.282SerIle: 3.282 ± 0.019	4.059SerLys: 4.059 ± 0.02	8.228SerLeu: 8.228 ± 0.034	1.611SerMet: 1.611 ± 0.014	2.734SerAsn: 2.734 ± 0.016	6.057SerPro: 6.057 ± 0.041	3.881SerGln: 3.881 ± 0.021	4.522SerArg: 4.522 ± 0.027	9.416SerSer: 9.416 ± 0.056	4.512SerThr: 4.512 ± 0.026	4.853SerVal: 4.853 ± 0.023	1.041SerTrp: 1.041 ± 0.009	2.136SerTyr: 2.136 ± 0.015	0.001SerXaa: 0.001 ± 0.0
Thr	3.682ThrAla: 3.682 ± 0.018	1.303ThrCys: 1.303 ± 0.016	2.46ThrAsp: 2.46 ± 0.015	3.502ThrGlu: 3.502 ± 0.019	2.123ThrPhe: 2.123 ± 0.015	3.497ThrGly: 3.497 ± 0.02	1.275ThrHis: 1.275 ± 0.011	2.489ThrIle: 2.489 ± 0.016	2.65ThrLys: 2.65 ± 0.017	5.358ThrLeu: 5.358 ± 0.022	1.137ThrMet: 1.137 ± 0.011	1.757ThrAsn: 1.757 ± 0.011	3.59ThrPro: 3.59 ± 0.023	2.286ThrGln: 2.286 ± 0.015	2.452ThrArg: 2.452 ± 0.014	4.612ThrSer: 4.612 ± 0.023	2.952ThrThr: 2.952 ± 0.018	3.859ThrVal: 3.859 ± 0.019	0.667ThrTrp: 0.667 ± 0.007	1.426ThrTyr: 1.426 ± 0.01	0.0ThrXaa: 0.0 ± 0.0
Val	4.29ValAla: 4.29 ± 0.022	1.412ValCys: 1.412 ± 0.013	2.955ValAsp: 2.955 ± 0.016	3.847ValGlu: 3.847 ± 0.02	2.432ValPhe: 2.432 ± 0.017	3.392ValGly: 3.392 ± 0.018	1.569ValHis: 1.569 ± 0.011	2.964ValIle: 2.964 ± 0.018	3.36ValLys: 3.36 ± 0.019	6.283ValLeu: 6.283 ± 0.026	1.335ValMet: 1.335 ± 0.01	2.28ValAsn: 2.28 ± 0.014	3.692ValPro: 3.692 ± 0.022	2.719ValGln: 2.719 ± 0.016	3.002ValArg: 3.002 ± 0.016	4.823ValSer: 4.823 ± 0.02	3.742ValThr: 3.742 ± 0.021	4.032ValVal: 4.032 ± 0.022	0.691ValTrp: 0.691 ± 0.008	1.625ValTyr: 1.625 ± 0.011	0.0ValXaa: 0.0 ± 0.0
Trp	0.772TrpAla: 0.772 ± 0.009	0.233TrpCys: 0.233 ± 0.005	0.644TrpAsp: 0.644 ± 0.007	0.789TrpGlu: 0.789 ± 0.009	0.442TrpPhe: 0.442 ± 0.006	0.715TrpGly: 0.715 ± 0.009	0.294TrpHis: 0.294 ± 0.005	0.553TrpIle: 0.553 ± 0.007	0.795TrpLys: 0.795 ± 0.008	1.187TrpLeu: 1.187 ± 0.009	0.312TrpMet: 0.312 ± 0.005	0.539TrpAsn: 0.539 ± 0.007	0.51TrpPro: 0.51 ± 0.006	0.527TrpGln: 0.527 ± 0.007	0.707TrpArg: 0.707 ± 0.008	0.851TrpSer: 0.851 ± 0.009	0.663TrpThr: 0.663 ± 0.008	0.697TrpVal: 0.697 ± 0.007	0.194TrpTrp: 0.194 ± 0.004	0.334TrpTyr: 0.334 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.428TyrAla: 1.428 ± 0.011	0.683TyrCys: 0.683 ± 0.009	1.324TyrAsp: 1.324 ± 0.01	1.708TyrGlu: 1.708 ± 0.014	1.282TyrPhe: 1.282 ± 0.012	1.717TyrGly: 1.717 ± 0.016	0.775TyrHis: 0.775 ± 0.008	1.405TyrIle: 1.405 ± 0.013	1.48TyrLys: 1.48 ± 0.022	2.714TyrLeu: 2.714 ± 0.016	0.615TyrMet: 0.615 ± 0.007	1.12TyrAsn: 1.12 ± 0.009	1.301TyrPro: 1.301 ± 0.01	1.259TyrGln: 1.259 ± 0.009	1.592TyrArg: 1.592 ± 0.012	2.228TyrSer: 2.228 ± 0.014	1.513TyrThr: 1.513 ± 0.012	1.622TyrVal: 1.622 ± 0.011	0.369TyrTrp: 0.369 ± 0.007	0.963TyrTyr: 0.963 ± 0.009	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.254XaaXaa: 0.254 ± 0.062

Statistics based on 25333 proteins (14498878 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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