Polynucleobacter victoriensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Polynucleobacter

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1630 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A212T8G4|A0A212T8G4_9BURK Uncharacterized protein OS=Polynucleobacter victoriensis OX=2049319 GN=SAMN06295916_0606 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 5.64FKK4 pKa = 10.31TYY6 pKa = 9.33MCLICGWVYY15 pKa = 11.09DD16 pKa = 4.61EE17 pKa = 4.94EE18 pKa = 6.63AGLPDD23 pKa = 3.89EE24 pKa = 5.79GIAPGTLWKK33 pKa = 10.24DD34 pKa = 3.42VPMNWTCPEE43 pKa = 3.86CGARR47 pKa = 11.84KK48 pKa = 9.49EE49 pKa = 4.08DD50 pKa = 4.27FEE52 pKa = 4.53MTVII56 pKa = 4.57

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A212TC96|A0A212TC96_9BURK Phosphoenolpyruvate carboxykinase [GTP] OS=Polynucleobacter victoriensis OX=2049319 GN=pckG PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TKK25 pKa = 10.35GGRR28 pKa = 11.84HH29 pKa = 4.61VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1630

0

1630

508469

32

1576

311.9

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.642 ± 0.07

0.946 ± 0.018

5.334 ± 0.039

5.832 ± 0.048

3.822 ± 0.04

7.41 ± 0.053

2.262 ± 0.027

6.431 ± 0.045

5.975 ± 0.049

10.268 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.028

3.805 ± 0.031

4.375 ± 0.037

4.161 ± 0.037

4.865 ± 0.05

6.074 ± 0.042

5.054 ± 0.034

7.104 ± 0.046

1.319 ± 0.023

2.655 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski