Amino acid dipepetide frequency for Polynucleobacter victoriensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.458AlaAla: 9.458 ± 0.183
1.038AlaCys: 1.038 ± 0.054
4.925AlaAsp: 4.925 ± 0.102
5.648AlaGlu: 5.648 ± 0.112
3.355AlaPhe: 3.355 ± 0.081
7.112AlaGly: 7.112 ± 0.128
2.01AlaHis: 2.01 ± 0.068
6.5AlaIle: 6.5 ± 0.111
6.691AlaLys: 6.691 ± 0.131
10.048AlaLeu: 10.048 ± 0.16
3.021AlaMet: 3.021 ± 0.089
3.709AlaAsn: 3.709 ± 0.086
3.587AlaPro: 3.587 ± 0.093
4.197AlaGln: 4.197 ± 0.096
4.561AlaArg: 4.561 ± 0.101
5.416AlaSer: 5.416 ± 0.1
4.753AlaThr: 4.753 ± 0.11
6.421AlaVal: 6.421 ± 0.14
1.392AlaTrp: 1.392 ± 0.059
2.582AlaTyr: 2.582 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
0.795CysAla: 0.795 ± 0.04
0.118CysCys: 0.118 ± 0.016
0.557CysAsp: 0.557 ± 0.033
0.523CysGlu: 0.523 ± 0.037
0.354CysPhe: 0.354 ± 0.028
0.915CysGly: 0.915 ± 0.049
0.319CysHis: 0.319 ± 0.029
0.541CysIle: 0.541 ± 0.037
0.393CysLys: 0.393 ± 0.027
0.94CysLeu: 0.94 ± 0.049
0.242CysMet: 0.242 ± 0.023
0.289CysAsn: 0.289 ± 0.025
0.562CysPro: 0.562 ± 0.04
0.391CysGln: 0.391 ± 0.026
0.393CysArg: 0.393 ± 0.034
0.659CysSer: 0.659 ± 0.039
0.46CysThr: 0.46 ± 0.03
0.657CysVal: 0.657 ± 0.043
0.11CysTrp: 0.11 ± 0.015
0.244CysTyr: 0.244 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
5.365AspAla: 5.365 ± 0.116
0.498AspCys: 0.498 ± 0.03
2.622AspAsp: 2.622 ± 0.089
3.277AspGlu: 3.277 ± 0.077
2.256AspPhe: 2.256 ± 0.071
3.961AspGly: 3.961 ± 0.085
1.196AspHis: 1.196 ± 0.058
3.455AspIle: 3.455 ± 0.074
2.818AspLys: 2.818 ± 0.084
5.898AspLeu: 5.898 ± 0.126
1.394AspMet: 1.394 ± 0.046
1.516AspAsn: 1.516 ± 0.052
2.433AspPro: 2.433 ± 0.073
2.23AspGln: 2.23 ± 0.059
2.726AspArg: 2.726 ± 0.071
2.726AspSer: 2.726 ± 0.068
2.297AspThr: 2.297 ± 0.064
4.087AspVal: 4.087 ± 0.099
0.964AspTrp: 0.964 ± 0.04
1.619AspTyr: 1.619 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
5.778GluAla: 5.778 ± 0.125
0.462GluCys: 0.462 ± 0.032
2.726GluAsp: 2.726 ± 0.077
3.288GluGlu: 3.288 ± 0.118
2.144GluPhe: 2.144 ± 0.062
3.597GluGly: 3.597 ± 0.088
1.355GluHis: 1.355 ± 0.052
4.136GluIle: 4.136 ± 0.095
4.069GluLys: 4.069 ± 0.092
6.325GluLeu: 6.325 ± 0.115
1.54GluMet: 1.54 ± 0.057
2.179GluAsn: 2.179 ± 0.073
2.053GluPro: 2.053 ± 0.071
2.661GluGln: 2.661 ± 0.08
3.255GluArg: 3.255 ± 0.088
3.294GluSer: 3.294 ± 0.086
2.897GluThr: 2.897 ± 0.082
4.34GluVal: 4.34 ± 0.1
0.753GluTrp: 0.753 ± 0.04
1.467GluTyr: 1.467 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.601PheAla: 3.601 ± 0.08
0.46PheCys: 0.46 ± 0.028
2.395PheAsp: 2.395 ± 0.068
2.293PheGlu: 2.293 ± 0.065
1.717PhePhe: 1.717 ± 0.068
3.304PheGly: 3.304 ± 0.098
0.775PheHis: 0.775 ± 0.043
2.458PheIle: 2.458 ± 0.07
2.167PheLys: 2.167 ± 0.066
3.568PheLeu: 3.568 ± 0.098
0.913PheMet: 0.913 ± 0.048
1.544PheAsn: 1.544 ± 0.062
1.526PhePro: 1.526 ± 0.057
1.202PheGln: 1.202 ± 0.05
1.528PheArg: 1.528 ± 0.057
2.508PheSer: 2.508 ± 0.077
2.047PheThr: 2.047 ± 0.069
2.651PheVal: 2.651 ± 0.072
0.49PheTrp: 0.49 ± 0.035
1.074PheTyr: 1.074 ± 0.047
0.0PheXaa: 0.0 ± 0.0
Gly
6.244GlyAla: 6.244 ± 0.138
0.863GlyCys: 0.863 ± 0.048
3.719GlyAsp: 3.719 ± 0.098
4.085GlyGlu: 4.085 ± 0.098
3.286GlyPhe: 3.286 ± 0.09
5.692GlyGly: 5.692 ± 0.137
1.851GlyHis: 1.851 ± 0.069
5.08GlyIle: 5.08 ± 0.101
4.832GlyLys: 4.832 ± 0.105
8.018GlyLeu: 8.018 ± 0.133
2.152GlyMet: 2.152 ± 0.071
2.415GlyAsn: 2.415 ± 0.075
2.285GlyPro: 2.285 ± 0.065
3.188GlyGln: 3.188 ± 0.076
3.568GlyArg: 3.568 ± 0.089
4.303GlySer: 4.303 ± 0.101
3.507GlyThr: 3.507 ± 0.084
5.739GlyVal: 5.739 ± 0.111
1.013GlyTrp: 1.013 ± 0.051
2.258GlyTyr: 2.258 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
2.195HisAla: 2.195 ± 0.065
0.309HisCys: 0.309 ± 0.025
1.225HisAsp: 1.225 ± 0.056
1.231HisGlu: 1.231 ± 0.045
0.885HisPhe: 0.885 ± 0.044
1.744HisGly: 1.744 ± 0.063
0.808HisHis: 0.808 ± 0.042
1.398HisIle: 1.398 ± 0.055
0.863HisLys: 0.863 ± 0.038
2.39HisLeu: 2.39 ± 0.081
0.527HisMet: 0.527 ± 0.033
0.635HisAsn: 0.635 ± 0.034
1.4HisPro: 1.4 ± 0.059
1.164HisGln: 1.164 ± 0.056
1.17HisArg: 1.17 ± 0.049
1.068HisSer: 1.068 ± 0.042
1.029HisThr: 1.029 ± 0.043
1.506HisVal: 1.506 ± 0.059
0.362HisTrp: 0.362 ± 0.027
0.708HisTyr: 0.708 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
7.031IleAla: 7.031 ± 0.126
0.688IleCys: 0.688 ± 0.042
4.187IleAsp: 4.187 ± 0.092
4.335IleGlu: 4.335 ± 0.085
2.277IlePhe: 2.277 ± 0.076
4.934IleGly: 4.934 ± 0.123
1.379IleHis: 1.379 ± 0.048
3.312IleIle: 3.312 ± 0.082
3.8IleLys: 3.8 ± 0.1
5.821IleLeu: 5.821 ± 0.124
1.249IleMet: 1.249 ± 0.059
2.698IleAsn: 2.698 ± 0.077
2.985IlePro: 2.985 ± 0.083
2.519IleGln: 2.519 ± 0.074
2.877IleArg: 2.877 ± 0.07
4.226IleSer: 4.226 ± 0.096
3.532IleThr: 3.532 ± 0.091
4.022IleVal: 4.022 ± 0.084
0.724IleTrp: 0.724 ± 0.042
1.713IleTyr: 1.713 ± 0.063
0.0IleXaa: 0.0 ± 0.0
Lys
5.758LysAla: 5.758 ± 0.125
0.323LysCys: 0.323 ± 0.027
3.361LysAsp: 3.361 ± 0.101
3.788LysGlu: 3.788 ± 0.087
1.678LysPhe: 1.678 ± 0.059
3.589LysGly: 3.589 ± 0.091
1.353LysHis: 1.353 ± 0.054
3.741LysIle: 3.741 ± 0.084
4.221LysLys: 4.221 ± 0.139
5.629LysLeu: 5.629 ± 0.123
1.735LysMet: 1.735 ± 0.062
2.871LysAsn: 2.871 ± 0.073
2.997LysPro: 2.997 ± 0.091
2.36LysGln: 2.36 ± 0.07
2.972LysArg: 2.972 ± 0.071
3.69LysSer: 3.69 ± 0.092
3.446LysThr: 3.446 ± 0.081
4.291LysVal: 4.291 ± 0.096
0.564LysTrp: 0.564 ± 0.034
1.381LysTyr: 1.381 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
10.799LeuAla: 10.799 ± 0.166
0.918LeuCys: 0.918 ± 0.045
5.574LeuAsp: 5.574 ± 0.114
5.847LeuGlu: 5.847 ± 0.114
3.811LeuPhe: 3.811 ± 0.099
7.778LeuGly: 7.778 ± 0.136
2.061LeuHis: 2.061 ± 0.068
6.608LeuIle: 6.608 ± 0.132
6.126LeuLys: 6.126 ± 0.129
9.859LeuLeu: 9.859 ± 0.185
2.683LeuMet: 2.683 ± 0.067
4.179LeuAsn: 4.179 ± 0.094
5.143LeuPro: 5.143 ± 0.128
3.646LeuGln: 3.646 ± 0.088
5.202LeuArg: 5.202 ± 0.129
6.962LeuSer: 6.962 ± 0.133
5.141LeuThr: 5.141 ± 0.098
6.97LeuVal: 6.97 ± 0.122
1.141LeuTrp: 1.141 ± 0.055
2.228LeuTyr: 2.228 ± 0.063
0.0LeuXaa: 0.0 ± 0.0
Met
2.844MetAla: 2.844 ± 0.071
0.193MetCys: 0.193 ± 0.019
1.235MetAsp: 1.235 ± 0.044
1.151MetGlu: 1.151 ± 0.05
0.824MetPhe: 0.824 ± 0.042
2.028MetGly: 2.028 ± 0.074
0.555MetHis: 0.555 ± 0.03
1.491MetIle: 1.491 ± 0.054
1.628MetLys: 1.628 ± 0.064
2.315MetLeu: 2.315 ± 0.077
0.789MetMet: 0.789 ± 0.044
1.137MetAsn: 1.137 ± 0.047
1.571MetPro: 1.571 ± 0.06
1.117MetGln: 1.117 ± 0.05
1.514MetArg: 1.514 ± 0.053
2.077MetSer: 2.077 ± 0.065
1.707MetThr: 1.707 ± 0.052
1.742MetVal: 1.742 ± 0.065
0.244MetTrp: 0.244 ± 0.023
0.5MetTyr: 0.5 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.55AsnAla: 3.55 ± 0.079
0.338AsnCys: 0.338 ± 0.025
1.8AsnAsp: 1.8 ± 0.062
1.9AsnGlu: 1.9 ± 0.053
1.422AsnPhe: 1.422 ± 0.056
2.576AsnGly: 2.576 ± 0.067
0.907AsnHis: 0.907 ± 0.043
2.46AsnIle: 2.46 ± 0.065
2.181AsnLys: 2.181 ± 0.073
3.92AsnLeu: 3.92 ± 0.084
1.027AsnMet: 1.027 ± 0.04
1.493AsnAsn: 1.493 ± 0.055
2.325AsnPro: 2.325 ± 0.063
2.081AsnGln: 2.081 ± 0.084
1.9AsnArg: 1.9 ± 0.068
2.152AsnSer: 2.152 ± 0.066
2.024AsnThr: 2.024 ± 0.061
2.346AsnVal: 2.346 ± 0.074
0.596AsnTrp: 0.596 ± 0.038
1.054AsnTyr: 1.054 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
3.802ProAla: 3.802 ± 0.115
0.364ProCys: 0.364 ± 0.025
2.504ProAsp: 2.504 ± 0.073
3.241ProGlu: 3.241 ± 0.077
1.621ProPhe: 1.621 ± 0.053
3.457ProGly: 3.457 ± 0.078
0.966ProHis: 0.966 ± 0.045
2.777ProIle: 2.777 ± 0.074
2.628ProLys: 2.628 ± 0.067
4.063ProLeu: 4.063 ± 0.094
1.17ProMet: 1.17 ± 0.045
1.953ProAsn: 1.953 ± 0.063
1.438ProPro: 1.438 ± 0.066
1.699ProGln: 1.699 ± 0.057
1.742ProArg: 1.742 ± 0.063
2.742ProSer: 2.742 ± 0.077
2.386ProThr: 2.386 ± 0.07
3.373ProVal: 3.373 ± 0.096
0.588ProTrp: 0.588 ± 0.033
1.241ProTyr: 1.241 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
4.474GlnAla: 4.474 ± 0.11
0.301GlnCys: 0.301 ± 0.024
1.935GlnAsp: 1.935 ± 0.065
2.445GlnGlu: 2.445 ± 0.077
1.536GlnPhe: 1.536 ± 0.049
2.761GlnGly: 2.761 ± 0.08
0.975GlnHis: 0.975 ± 0.043
2.746GlnIle: 2.746 ± 0.074
2.474GlnLys: 2.474 ± 0.069
4.563GlnLeu: 4.563 ± 0.123
1.115GlnMet: 1.115 ± 0.048
1.449GlnAsn: 1.449 ± 0.054
1.508GlnPro: 1.508 ± 0.057
1.961GlnGln: 1.961 ± 0.072
2.059GlnArg: 2.059 ± 0.063
2.592GlnSer: 2.592 ± 0.076
1.994GlnThr: 1.994 ± 0.061
3.212GlnVal: 3.212 ± 0.082
0.474GlnTrp: 0.474 ± 0.03
1.036GlnTyr: 1.036 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
4.256ArgAla: 4.256 ± 0.104
0.385ArgCys: 0.385 ± 0.028
2.62ArgAsp: 2.62 ± 0.073
3.027ArgGlu: 3.027 ± 0.078
2.1ArgPhe: 2.1 ± 0.065
3.18ArgGly: 3.18 ± 0.085
1.182ArgHis: 1.182 ± 0.047
3.214ArgIle: 3.214 ± 0.074
2.502ArgLys: 2.502 ± 0.068
5.359ArgLeu: 5.359 ± 0.112
1.343ArgMet: 1.343 ± 0.054
1.819ArgAsn: 1.819 ± 0.061
1.961ArgPro: 1.961 ± 0.067
2.207ArgGln: 2.207 ± 0.068
2.622ArgArg: 2.622 ± 0.086
2.785ArgSer: 2.785 ± 0.083
2.134ArgThr: 2.134 ± 0.072
3.599ArgVal: 3.599 ± 0.082
0.692ArgTrp: 0.692 ± 0.036
1.666ArgTyr: 1.666 ± 0.064
0.0ArgXaa: 0.0 ± 0.0
Ser
5.351SerAla: 5.351 ± 0.108
0.566SerCys: 0.566 ± 0.036
3.273SerAsp: 3.273 ± 0.083
3.363SerGlu: 3.363 ± 0.081
2.582SerPhe: 2.582 ± 0.076
5.041SerGly: 5.041 ± 0.1
1.381SerHis: 1.381 ± 0.052
3.935SerIle: 3.935 ± 0.102
3.564SerLys: 3.564 ± 0.084
6.447SerLeu: 6.447 ± 0.118
1.599SerMet: 1.599 ± 0.057
2.46SerAsn: 2.46 ± 0.065
2.464SerPro: 2.464 ± 0.076
2.527SerGln: 2.527 ± 0.065
2.73SerArg: 2.73 ± 0.078
3.924SerSer: 3.924 ± 0.093
2.974SerThr: 2.974 ± 0.074
3.979SerVal: 3.979 ± 0.099
0.838SerTrp: 0.838 ± 0.038
1.742SerTyr: 1.742 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
4.594ThrAla: 4.594 ± 0.092
0.464ThrCys: 0.464 ± 0.032
2.606ThrAsp: 2.606 ± 0.073
2.714ThrGlu: 2.714 ± 0.076
1.919ThrPhe: 1.919 ± 0.057
4.167ThrGly: 4.167 ± 0.102
1.231ThrHis: 1.231 ± 0.044
3.245ThrIle: 3.245 ± 0.078
2.647ThrLys: 2.647 ± 0.075
5.483ThrLeu: 5.483 ± 0.103
1.131ThrMet: 1.131 ± 0.047
1.904ThrAsn: 1.904 ± 0.066
2.797ThrPro: 2.797 ± 0.075
2.016ThrGln: 2.016 ± 0.078
2.323ThrArg: 2.323 ± 0.068
2.974ThrSer: 2.974 ± 0.073
2.509ThrThr: 2.509 ± 0.078
3.69ThrVal: 3.69 ± 0.084
0.71ThrTrp: 0.71 ± 0.041
1.418ThrTyr: 1.418 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
7.07ValAla: 7.07 ± 0.137
0.702ValCys: 0.702 ± 0.035
4.089ValAsp: 4.089 ± 0.076
4.11ValGlu: 4.11 ± 0.106
2.667ValPhe: 2.667 ± 0.069
5.259ValGly: 5.259 ± 0.117
1.385ValHis: 1.385 ± 0.057
5.037ValIle: 5.037 ± 0.113
4.097ValLys: 4.097 ± 0.107
7.243ValLeu: 7.243 ± 0.134
2.095ValMet: 2.095 ± 0.064
2.578ValAsn: 2.578 ± 0.071
2.974ValPro: 2.974 ± 0.079
2.295ValGln: 2.295 ± 0.065
3.277ValArg: 3.277 ± 0.083
4.299ValSer: 4.299 ± 0.09
3.882ValThr: 3.882 ± 0.093
5.522ValVal: 5.522 ± 0.127
0.877ValTrp: 0.877 ± 0.044
1.579ValTyr: 1.579 ± 0.065
0.0ValXaa: 0.0 ± 0.0
Trp
1.093TrpAla: 1.093 ± 0.048
0.161TrpCys: 0.161 ± 0.014
0.602TrpAsp: 0.602 ± 0.036
0.6TrpGlu: 0.6 ± 0.039
0.604TrpPhe: 0.604 ± 0.037
0.85TrpGly: 0.85 ± 0.042
0.368TrpHis: 0.368 ± 0.029
0.757TrpIle: 0.757 ± 0.044
0.616TrpLys: 0.616 ± 0.038
1.766TrpLeu: 1.766 ± 0.068
0.393TrpMet: 0.393 ± 0.028
0.433TrpAsn: 0.433 ± 0.03
0.513TrpPro: 0.513 ± 0.035
0.751TrpGln: 0.751 ± 0.042
0.773TrpArg: 0.773 ± 0.04
0.763TrpSer: 0.763 ± 0.038
0.586TrpThr: 0.586 ± 0.039
1.007TrpVal: 1.007 ± 0.044
0.238TrpTrp: 0.238 ± 0.023
0.319TrpTyr: 0.319 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.366TyrAla: 2.366 ± 0.071
0.309TyrCys: 0.309 ± 0.026
1.385TyrAsp: 1.385 ± 0.056
1.455TyrGlu: 1.455 ± 0.053
1.182TyrPhe: 1.182 ± 0.047
2.152TyrGly: 2.152 ± 0.062
0.564TyrHis: 0.564 ± 0.03
1.422TyrIle: 1.422 ± 0.051
1.43TyrLys: 1.43 ± 0.057
3.023TyrLeu: 3.023 ± 0.075
0.543TyrMet: 0.543 ± 0.028
0.787TyrAsn: 0.787 ± 0.035
1.229TyrPro: 1.229 ± 0.052
1.398TyrGln: 1.398 ± 0.059
1.461TyrArg: 1.461 ± 0.059
1.581TyrSer: 1.581 ± 0.055
1.237TyrThr: 1.237 ± 0.043
1.882TyrVal: 1.882 ± 0.066
0.423TyrTrp: 0.423 ± 0.025
0.72TyrTyr: 0.72 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1630 proteins (508469 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski