Halopenitus malekzadehii
Average proteome isoelectric point is 4.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3119 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H6I0M3|A0A1H6I0M3_9EURY Inosose dehydratase OS=Halopenitus malekzadehii OX=1267564 GN=SAMN05192561_101850 PE=4 SV=1
MM1 pKa = 7.44 EE2 pKa = 4.86 LTWHH6 pKa = 6.23 GHH8 pKa = 4.5 STWHH12 pKa = 5.64 VSVGEE17 pKa = 4.05 TDD19 pKa = 4.61 LLIDD23 pKa = 4.7 PFFDD27 pKa = 3.66 NPKK30 pKa = 8.95 TDD32 pKa = 3.31 VSPEE36 pKa = 4.0 EE37 pKa = 4.61 LDD39 pKa = 3.43 PDD41 pKa = 4.2 YY42 pKa = 11.68 LLLTHH47 pKa = 6.39 GHH49 pKa = 7.12 ADD51 pKa = 3.47 HH52 pKa = 6.97 IGDD55 pKa = 3.38 VDD57 pKa = 4.04 RR58 pKa = 11.84 YY59 pKa = 10.68 EE60 pKa = 4.71 DD61 pKa = 3.6 ATLVATPEE69 pKa = 4.07 LVGYY73 pKa = 9.54 VQDD76 pKa = 4.05 TFGHH80 pKa = 6.67 DD81 pKa = 3.44 SAVGGMGMNLGGTVEE96 pKa = 4.54 CGDD99 pKa = 3.9 AFVTMVRR106 pKa = 11.84 ADD108 pKa = 3.54 HH109 pKa = 6.9 SNGIDD114 pKa = 3.12 TGYY117 pKa = 8.63 GTSAGMPAGFVVSDD131 pKa = 3.9 SKK133 pKa = 10.4 PTQEE137 pKa = 4.52 SDD139 pKa = 3.39 AEE141 pKa = 4.34 SSTFYY146 pKa = 10.72 HH147 pKa = 7.1 AGDD150 pKa = 3.45 TSLMVEE156 pKa = 4.09 MRR158 pKa = 11.84 EE159 pKa = 4.17 VIGPFLEE166 pKa = 4.65 PDD168 pKa = 3.65 VAALPAGDD176 pKa = 4.73 HH177 pKa = 5.58 FTMGPAQAAIAADD190 pKa = 3.54 WVGADD195 pKa = 3.4 VAIPMHH201 pKa = 6.7 YY202 pKa = 10.59 DD203 pKa = 3.25 SFPPIEE209 pKa = 5.17 IDD211 pKa = 2.8 TDD213 pKa = 3.77 DD214 pKa = 3.75 FVRR217 pKa = 11.84 EE218 pKa = 4.12 VEE220 pKa = 4.28 ATAGPSEE227 pKa = 4.09 PVVLEE232 pKa = 3.82 GDD234 pKa = 3.37 EE235 pKa = 4.4 SYY237 pKa = 11.15 EE238 pKa = 3.83 II239 pKa = 4.44
Molecular weight: 25.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.63
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.528
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.164
Thurlkill 3.668
EMBOSS 3.757
Sillero 3.961
Patrickios 1.227
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.845
Protein with the highest isoelectric point:
>tr|A0A1H6IUY4|A0A1H6IUY4_9EURY Uncharacterized protein OS=Halopenitus malekzadehii OX=1267564 GN=SAMN05192561_103196 PE=4 SV=1
MM1 pKa = 7.03 TSVRR5 pKa = 11.84 TRR7 pKa = 11.84 LALRR11 pKa = 11.84 TRR13 pKa = 11.84 GNSRR17 pKa = 11.84 VTGLKK22 pKa = 10.05 RR23 pKa = 11.84 IVPAVLSWGIDD34 pKa = 3.06 SCLALIVKK42 pKa = 8.25 FYY44 pKa = 11.11 QRR46 pKa = 11.84 VLTGPLFTIIGIEE59 pKa = 3.95 RR60 pKa = 11.84 LKK62 pKa = 11.21 HH63 pKa = 5.57 FVAEE67 pKa = 4.68 FNQFRR72 pKa = 11.84 ISCHH76 pKa = 5.32 DD77 pKa = 3.55 TVNEE81 pKa = 4.17 CVITT85 pKa = 3.9
Molecular weight: 9.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.428
IPC_protein 10.131
Toseland 10.599
ProMoST 10.277
Dawson 10.687
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.847
Grimsley 10.716
Solomon 10.818
Lehninger 10.804
Nozaki 10.643
DTASelect 10.379
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.628
Patrickios 10.672
IPC_peptide 10.833
IPC2_peptide 9.809
IPC2.peptide.svr19 8.501
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3119
0
3119
899592
29
2049
288.4
31.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.071 ± 0.068
0.721 ± 0.023
8.781 ± 0.062
8.18 ± 0.064
3.172 ± 0.03
8.599 ± 0.041
2.051 ± 0.021
4.6 ± 0.034
1.72 ± 0.028
8.588 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.638 ± 0.021
2.304 ± 0.029
4.766 ± 0.03
2.263 ± 0.028
6.736 ± 0.046
5.485 ± 0.033
6.804 ± 0.037
8.843 ± 0.049
1.084 ± 0.017
2.591 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here