Candidatus Planktophila sulfonica
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1334 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A249KHI8|A0A249KHI8_9ACTN Probable dual-specificity RNA methyltransferase RlmN OS=Candidatus Planktophila sulfonica OX=1884904 GN=rlmN PE=3 SV=1
MM1 pKa = 7.5 TYY3 pKa = 10.56 VIAEE7 pKa = 4.13 PCVDD11 pKa = 4.46 VKK13 pKa = 11.25 DD14 pKa = 3.84 KK15 pKa = 10.98 SCRR18 pKa = 11.84 EE19 pKa = 4.08 EE20 pKa = 4.76 CPVDD24 pKa = 4.26 CIYY27 pKa = 11.27 DD28 pKa = 3.85 GTRR31 pKa = 11.84 MLYY34 pKa = 9.42 IQPDD38 pKa = 3.57 EE39 pKa = 4.8 CVDD42 pKa = 3.84 CGACEE47 pKa = 3.85 PVCPVEE53 pKa = 4.4 AIYY56 pKa = 11.25 YY57 pKa = 9.23 EE58 pKa = 4.68 DD59 pKa = 5.0 DD60 pKa = 5.7 VPGQWQNYY68 pKa = 7.64 KK69 pKa = 10.51 KK70 pKa = 10.57 INTEE74 pKa = 3.74 FFIEE78 pKa = 4.29 LGSPGGASKK87 pKa = 10.85 VGEE90 pKa = 4.6 TNTDD94 pKa = 3.47 HH95 pKa = 7.81 ADD97 pKa = 3.4 VTALGPQTSS106 pKa = 3.33
Molecular weight: 11.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.946
IPC2_protein 4.024
IPC_protein 3.948
Toseland 3.757
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.846
Rodwell 3.783
Grimsley 3.681
Solomon 3.923
Lehninger 3.872
Nozaki 4.05
DTASelect 4.228
Thurlkill 3.808
EMBOSS 3.859
Sillero 4.075
Patrickios 0.172
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.975
Protein with the highest isoelectric point:
>tr|A0A249KII2|A0A249KII2_9ACTN D-xylose transport system substrate-binding protein OS=Candidatus Planktophila sulfonica OX=1884904 GN=A1sIA56_01185 PE=4 SV=1
MM1 pKa = 7.51 TKK3 pKa = 9.08 RR4 pKa = 11.84 TFQPNTRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 10.1 KK16 pKa = 8.61 HH17 pKa = 4.41 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATRR26 pKa = 11.84 AGRR29 pKa = 11.84 AVLSARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.41 GRR40 pKa = 11.84 SKK42 pKa = 11.12 LSAA45 pKa = 3.74
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1334
0
1334
425275
37
1505
318.8
34.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.862 ± 0.086
0.7 ± 0.017
5.447 ± 0.055
6.029 ± 0.077
3.727 ± 0.047
8.099 ± 0.054
1.955 ± 0.032
6.581 ± 0.055
4.893 ± 0.054
9.677 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.189 ± 0.026
3.148 ± 0.033
4.412 ± 0.038
2.955 ± 0.027
5.381 ± 0.062
6.689 ± 0.06
5.925 ± 0.047
7.779 ± 0.056
1.224 ± 0.026
2.33 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here