Candidatus Planktophila sulfonica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Candidatus Nanopelagicales; Candidatus Nanopelagicaceae; Candidatus Planktophila

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A249KHI8|A0A249KHI8_9ACTN Probable dual-specificity RNA methyltransferase RlmN OS=Candidatus Planktophila sulfonica OX=1884904 GN=rlmN PE=3 SV=1
MM1 pKa = 7.5TYY3 pKa = 10.56VIAEE7 pKa = 4.13PCVDD11 pKa = 4.46VKK13 pKa = 11.25DD14 pKa = 3.84KK15 pKa = 10.98SCRR18 pKa = 11.84EE19 pKa = 4.08EE20 pKa = 4.76CPVDD24 pKa = 4.26CIYY27 pKa = 11.27DD28 pKa = 3.85GTRR31 pKa = 11.84MLYY34 pKa = 9.42IQPDD38 pKa = 3.57EE39 pKa = 4.8CVDD42 pKa = 3.84CGACEE47 pKa = 3.85PVCPVEE53 pKa = 4.4AIYY56 pKa = 11.25YY57 pKa = 9.23EE58 pKa = 4.68DD59 pKa = 5.0DD60 pKa = 5.7VPGQWQNYY68 pKa = 7.64KK69 pKa = 10.51KK70 pKa = 10.57INTEE74 pKa = 3.74FFIEE78 pKa = 4.29LGSPGGASKK87 pKa = 10.85VGEE90 pKa = 4.6TNTDD94 pKa = 3.47HH95 pKa = 7.81ADD97 pKa = 3.4VTALGPQTSS106 pKa = 3.33

Molecular weight:
11.68 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A249KII2|A0A249KII2_9ACTN D-xylose transport system substrate-binding protein OS=Candidatus Planktophila sulfonica OX=1884904 GN=A1sIA56_01185 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.08RR4 pKa = 11.84TFQPNTRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.1KK16 pKa = 8.61HH17 pKa = 4.41GFRR20 pKa = 11.84ARR22 pKa = 11.84MATRR26 pKa = 11.84AGRR29 pKa = 11.84AVLSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.41GRR40 pKa = 11.84SKK42 pKa = 11.12LSAA45 pKa = 3.74

Molecular weight:
5.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1334

0

1334

425275

37

1505

318.8

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.862 ± 0.086

0.7 ± 0.017

5.447 ± 0.055

6.029 ± 0.077

3.727 ± 0.047

8.099 ± 0.054

1.955 ± 0.032

6.581 ± 0.055

4.893 ± 0.054

9.677 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.026

3.148 ± 0.033

4.412 ± 0.038

2.955 ± 0.027

5.381 ± 0.062

6.689 ± 0.06

5.925 ± 0.047

7.779 ± 0.056

1.224 ± 0.026

2.33 ± 0.033

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski