Pseudodesulfovibrio profundus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Pseudodesulfovibrio

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3808 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2C8FBT4|A0A2C8FBT4_9DELT Endolytic murein transglycosylase OS=Pseudodesulfovibrio profundus OX=57320 GN=yceG PE=3 SV=1
MM1 pKa = 7.73EE2 pKa = 5.46NNNEE6 pKa = 4.42NINNMSRR13 pKa = 11.84DD14 pKa = 4.02FEE16 pKa = 4.65PEE18 pKa = 3.69SATDD22 pKa = 3.89FFVHH26 pKa = 7.18DD27 pKa = 4.01RR28 pKa = 11.84LEE30 pKa = 4.14NRR32 pKa = 11.84AEE34 pKa = 4.11PEE36 pKa = 4.23SVRR39 pKa = 11.84LQIDD43 pKa = 2.98WRR45 pKa = 11.84DD46 pKa = 3.17GSIDD50 pKa = 3.58VVTIAQHH57 pKa = 6.16QMNSWSSEE65 pKa = 3.68EE66 pKa = 3.63WHH68 pKa = 6.65GLVGVYY74 pKa = 7.84QVNYY78 pKa = 10.17DD79 pKa = 3.32ADD81 pKa = 3.8AFALAEE87 pKa = 4.11WVDD90 pKa = 4.04EE91 pKa = 4.04NMGMLSRR98 pKa = 11.84IHH100 pKa = 6.84ACFDD104 pKa = 3.55EE105 pKa = 4.97HH106 pKa = 6.93WDD108 pKa = 3.35GSNFVGEE115 pKa = 4.25FDD117 pKa = 4.3FDD119 pKa = 3.86NDD121 pKa = 3.27EE122 pKa = 4.11EE123 pKa = 5.93AGDD126 pKa = 3.92FLEE129 pKa = 6.44HH130 pKa = 6.71EE131 pKa = 4.28LQLYY135 pKa = 10.06GIMVEE140 pKa = 4.15SHH142 pKa = 5.47GVAMVDD148 pKa = 3.15AGDD151 pKa = 3.99YY152 pKa = 9.87FCEE155 pKa = 4.44SFWIQDD161 pKa = 3.8GMLHH165 pKa = 6.18TDD167 pKa = 4.4LRR169 pKa = 11.84DD170 pKa = 3.48TPYY173 pKa = 11.16SLSEE177 pKa = 4.29LGDD180 pKa = 3.38EE181 pKa = 4.86GEE183 pKa = 4.82YY184 pKa = 10.46ILEE187 pKa = 4.15ALMDD191 pKa = 4.13NDD193 pKa = 5.57KK194 pKa = 11.35SDD196 pKa = 3.85EE197 pKa = 4.15SQMAVCTLDD206 pKa = 3.39YY207 pKa = 10.88AKK209 pKa = 10.95EE210 pKa = 3.79LMYY213 pKa = 10.68DD214 pKa = 3.87LRR216 pKa = 11.84NACAEE221 pKa = 4.03

Molecular weight:
25.46 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2C8FEF2|A0A2C8FEF2_9DELT HTH asnC-type domain-containing protein OS=Pseudodesulfovibrio profundus OX=57320 GN=DPRO_4004 PE=4 SV=1
MM1 pKa = 7.7PKK3 pKa = 9.95IKK5 pKa = 9.47TRR7 pKa = 11.84RR8 pKa = 11.84AAAKK12 pKa = 9.63RR13 pKa = 11.84FSKK16 pKa = 9.46TASGKK21 pKa = 9.4FKK23 pKa = 10.67RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.07NLRR30 pKa = 11.84HH31 pKa = 6.24ILTKK35 pKa = 10.67KK36 pKa = 7.68NAKK39 pKa = 9.22RR40 pKa = 11.84KK41 pKa = 9.18RR42 pKa = 11.84RR43 pKa = 11.84LGQSTTVDD51 pKa = 3.67STNMKK56 pKa = 9.79AVRR59 pKa = 11.84RR60 pKa = 11.84QLPNGG65 pKa = 3.51

Molecular weight:
7.58 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3808

0

3808

1187153

20

3004

311.8

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.644 ± 0.036

1.268 ± 0.021

5.93 ± 0.029

6.89 ± 0.04

4.143 ± 0.03

7.596 ± 0.035

2.12 ± 0.019

6.013 ± 0.033

5.359 ± 0.041

9.879 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.978 ± 0.02

3.502 ± 0.024

4.409 ± 0.024

3.378 ± 0.025

5.543 ± 0.036

5.885 ± 0.031

5.252 ± 0.033

7.165 ± 0.039

1.205 ± 0.015

2.84 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski