Pseudodesulfovibrio profundus
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3808 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2C8FBT4|A0A2C8FBT4_9DELT Endolytic murein transglycosylase OS=Pseudodesulfovibrio profundus OX=57320 GN=yceG PE=3 SV=1
MM1 pKa = 7.73 EE2 pKa = 5.46 NNNEE6 pKa = 4.42 NINNMSRR13 pKa = 11.84 DD14 pKa = 4.02 FEE16 pKa = 4.65 PEE18 pKa = 3.69 SATDD22 pKa = 3.89 FFVHH26 pKa = 7.18 DD27 pKa = 4.01 RR28 pKa = 11.84 LEE30 pKa = 4.14 NRR32 pKa = 11.84 AEE34 pKa = 4.11 PEE36 pKa = 4.23 SVRR39 pKa = 11.84 LQIDD43 pKa = 2.98 WRR45 pKa = 11.84 DD46 pKa = 3.17 GSIDD50 pKa = 3.58 VVTIAQHH57 pKa = 6.16 QMNSWSSEE65 pKa = 3.68 EE66 pKa = 3.63 WHH68 pKa = 6.65 GLVGVYY74 pKa = 7.84 QVNYY78 pKa = 10.17 DD79 pKa = 3.32 ADD81 pKa = 3.8 AFALAEE87 pKa = 4.11 WVDD90 pKa = 4.04 EE91 pKa = 4.04 NMGMLSRR98 pKa = 11.84 IHH100 pKa = 6.84 ACFDD104 pKa = 3.55 EE105 pKa = 4.97 HH106 pKa = 6.93 WDD108 pKa = 3.35 GSNFVGEE115 pKa = 4.25 FDD117 pKa = 4.3 FDD119 pKa = 3.86 NDD121 pKa = 3.27 EE122 pKa = 4.11 EE123 pKa = 5.93 AGDD126 pKa = 3.92 FLEE129 pKa = 6.44 HH130 pKa = 6.71 EE131 pKa = 4.28 LQLYY135 pKa = 10.06 GIMVEE140 pKa = 4.15 SHH142 pKa = 5.47 GVAMVDD148 pKa = 3.15 AGDD151 pKa = 3.99 YY152 pKa = 9.87 FCEE155 pKa = 4.44 SFWIQDD161 pKa = 3.8 GMLHH165 pKa = 6.18 TDD167 pKa = 4.4 LRR169 pKa = 11.84 DD170 pKa = 3.48 TPYY173 pKa = 11.16 SLSEE177 pKa = 4.29 LGDD180 pKa = 3.38 EE181 pKa = 4.86 GEE183 pKa = 4.82 YY184 pKa = 10.46 ILEE187 pKa = 4.15 ALMDD191 pKa = 4.13 NDD193 pKa = 5.57 KK194 pKa = 11.35 SDD196 pKa = 3.85 EE197 pKa = 4.15 SQMAVCTLDD206 pKa = 3.39 YY207 pKa = 10.88 AKK209 pKa = 10.95 EE210 pKa = 3.79 LMYY213 pKa = 10.68 DD214 pKa = 3.87 LRR216 pKa = 11.84 NACAEE221 pKa = 4.03
Molecular weight: 25.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.63
ProMoST 3.961
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.719
Rodwell 3.668
Grimsley 3.541
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.961
Patrickios 1.252
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|A0A2C8FEF2|A0A2C8FEF2_9DELT HTH asnC-type domain-containing protein OS=Pseudodesulfovibrio profundus OX=57320 GN=DPRO_4004 PE=4 SV=1
MM1 pKa = 7.7 PKK3 pKa = 9.95 IKK5 pKa = 9.47 TRR7 pKa = 11.84 RR8 pKa = 11.84 AAAKK12 pKa = 9.63 RR13 pKa = 11.84 FSKK16 pKa = 9.46 TASGKK21 pKa = 9.4 FKK23 pKa = 10.67 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 KK27 pKa = 9.07 NLRR30 pKa = 11.84 HH31 pKa = 6.24 ILTKK35 pKa = 10.67 KK36 pKa = 7.68 NAKK39 pKa = 9.22 RR40 pKa = 11.84 KK41 pKa = 9.18 RR42 pKa = 11.84 RR43 pKa = 11.84 LGQSTTVDD51 pKa = 3.67 STNMKK56 pKa = 9.79 AVRR59 pKa = 11.84 RR60 pKa = 11.84 QLPNGG65 pKa = 3.51
Molecular weight: 7.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.544
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.266
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.04
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3808
0
3808
1187153
20
3004
311.8
34.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.644 ± 0.036
1.268 ± 0.021
5.93 ± 0.029
6.89 ± 0.04
4.143 ± 0.03
7.596 ± 0.035
2.12 ± 0.019
6.013 ± 0.033
5.359 ± 0.041
9.879 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.978 ± 0.02
3.502 ± 0.024
4.409 ± 0.024
3.378 ± 0.025
5.543 ± 0.036
5.885 ± 0.031
5.252 ± 0.033
7.165 ± 0.039
1.205 ± 0.015
2.84 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here