Arthrobacter phage RcigaStruga

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Korravirus; unclassified Korravirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9LZF6|A0A1C9LZF6_9CAUD Uncharacterized protein OS=Arthrobacter phage RcigaStruga OX=1897763 GN=SEA_RCIGASTRUGA_58 PE=4 SV=1
MM1 pKa = 7.41TRR3 pKa = 11.84GQGYY7 pKa = 8.82GLIEE11 pKa = 4.05FRR13 pKa = 11.84GADD16 pKa = 3.52SPSQPKK22 pKa = 8.95EE23 pKa = 3.69PPMINPAAAQLVEE36 pKa = 3.96QLNTIGAEE44 pKa = 3.92LEE46 pKa = 4.5RR47 pKa = 11.84IHH49 pKa = 6.37EE50 pKa = 4.65AEE52 pKa = 4.19GQEE55 pKa = 4.16PPLTTGEE62 pKa = 4.24VFVNVQTGRR71 pKa = 11.84VWFKK75 pKa = 10.94VQSQQDD81 pKa = 3.85GTEE84 pKa = 3.88LWLSEE89 pKa = 4.66HH90 pKa = 6.48KK91 pKa = 10.51RR92 pKa = 11.84HH93 pKa = 6.08AAPGEE98 pKa = 4.12TLVSVEE104 pKa = 4.3TVEE107 pKa = 3.97EE108 pKa = 4.49AIMYY112 pKa = 8.79EE113 pKa = 3.83WDD115 pKa = 3.08

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9LZB5|A0A1C9LZB5_9CAUD DNA helicase OS=Arthrobacter phage RcigaStruga OX=1897763 GN=SEA_RCIGASTRUGA_29 PE=4 SV=1
MM1 pKa = 7.4EE2 pKa = 5.19RR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 9.68LQPRR9 pKa = 11.84MSSKK13 pKa = 10.96DD14 pKa = 3.58YY15 pKa = 10.37VRR17 pKa = 11.84KK18 pKa = 10.32GGAKK22 pKa = 10.18SIRR25 pKa = 11.84FTADD29 pKa = 2.25YY30 pKa = 8.76WAALRR35 pKa = 11.84KK36 pKa = 9.12QRR38 pKa = 11.84QEE40 pKa = 3.81QEE42 pKa = 3.74AKK44 pKa = 9.44LLRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84VVAHH53 pKa = 6.16CRR55 pKa = 11.84FMDD58 pKa = 4.57RR59 pKa = 11.84DD60 pKa = 4.13GQPPLYY66 pKa = 10.52VFFGGAQEE74 pKa = 4.04LRR76 pKa = 11.84HH77 pKa = 5.55WSQDD81 pKa = 2.85FMLALLEE88 pKa = 4.26VGVGFEE94 pKa = 3.95PRR96 pKa = 11.84QDD98 pKa = 2.78IVAARR103 pKa = 11.84PEE105 pKa = 4.42RR106 pKa = 11.84IRR108 pKa = 11.84GHH110 pKa = 5.54RR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84LVAVWSSDD121 pKa = 3.15PTPVGEE127 pKa = 4.12RR128 pKa = 11.84ARR130 pKa = 11.84LAEE133 pKa = 4.77IDD135 pKa = 3.27TMYY138 pKa = 10.67CIEE141 pKa = 5.61HH142 pKa = 5.86MNQMNGYY149 pKa = 8.41EE150 pKa = 4.31VSRR153 pKa = 11.84EE154 pKa = 3.99TTNAA158 pKa = 3.22

Molecular weight: 18.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13939

32

869

224.8

24.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.309 ± 0.385

0.725 ± 0.124

5.653 ± 0.221

6.543 ± 0.358

2.992 ± 0.214

8.724 ± 0.378

1.93 ± 0.182

5.129 ± 0.333

5.036 ± 0.25

8.372 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.163

3.515 ± 0.174

5.101 ± 0.181

3.838 ± 0.157

5.603 ± 0.353

6.163 ± 0.232

6.493 ± 0.319

7.138 ± 0.255

2.002 ± 0.196

2.461 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski