Choiromyces venosus 120613-1
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17720 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4IY03|A0A3N4IY03_9PEZI Uncharacterized protein (Fragment) OS=Choiromyces venosus 120613-1 OX=1336337 GN=L873DRAFT_1848584 PE=4 SV=1
MM1 pKa = 7.8 PIEE4 pKa = 4.5 SDD6 pKa = 3.6 IKK8 pKa = 10.94 VEE10 pKa = 4.19 DD11 pKa = 4.27 NIVFMSYY18 pKa = 10.48 SDD20 pKa = 4.46 SIFTILDD27 pKa = 3.7 SNTSTDD33 pKa = 3.19 EE34 pKa = 4.1 EE35 pKa = 5.06 SIHH38 pKa = 6.64 EE39 pKa = 4.32 CDD41 pKa = 5.25 AYY43 pKa = 10.11 IDD45 pKa = 3.87 NNQRR49 pKa = 11.84 GPDD52 pKa = 3.44 SYY54 pKa = 11.0 IQNDD58 pKa = 3.69 QEE60 pKa = 4.82 SLNNPANSSSPWFQFEE76 pKa = 4.14 EE77 pKa = 4.44 SHH79 pKa = 6.4 TEE81 pKa = 3.54 SDD83 pKa = 3.93 TNFPEE88 pKa = 6.28 ADD90 pKa = 3.25 IQDD93 pKa = 3.73 KK94 pKa = 11.09 NEE96 pKa = 4.0 WYY98 pKa = 9.44 PFPSS102 pKa = 3.21
Molecular weight: 11.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.579
IPC_protein 3.541
Toseland 3.35
ProMoST 3.719
Dawson 3.541
Bjellqvist 3.706
Wikipedia 3.478
Rodwell 3.389
Grimsley 3.261
Solomon 3.516
Lehninger 3.478
Nozaki 3.668
DTASelect 3.859
Thurlkill 3.414
EMBOSS 3.49
Sillero 3.668
Patrickios 0.769
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.658
Protein with the highest isoelectric point:
>tr|A0A3N4J0K4|A0A3N4J0K4_9PEZI Uncharacterized protein OS=Choiromyces venosus 120613-1 OX=1336337 GN=L873DRAFT_402780 PE=4 SV=1
PP1 pKa = 7.74 PSLLQHH7 pKa = 6.82 RR8 pKa = 11.84 PPSLLQHH15 pKa = 6.78 RR16 pKa = 11.84 PPSLLQHH23 pKa = 6.78 RR24 pKa = 11.84 PPSLLQHH31 pKa = 6.78 RR32 pKa = 11.84 PPSLLQHH39 pKa = 6.78 RR40 pKa = 11.84 PPSLLQHH47 pKa = 6.78 RR48 pKa = 11.84 PPSLLQHH55 pKa = 6.78 RR56 pKa = 11.84 PPSLLQHH63 pKa = 6.78 RR64 pKa = 11.84 PPSLLQHH71 pKa = 6.78 RR72 pKa = 11.84 PPSLLQHH79 pKa = 6.78 RR80 pKa = 11.84 PPSLLQHH87 pKa = 6.78 RR88 pKa = 11.84 PPSLLQHH95 pKa = 6.78 RR96 pKa = 11.84 PPSLLQHH103 pKa = 6.79 RR104 pKa = 11.84 PPP106 pKa = 4.75
Molecular weight: 12.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.31
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.257
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17720
0
17720
5377989
49
5694
303.5
33.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.443 ± 0.017
1.401 ± 0.01
5.136 ± 0.015
6.518 ± 0.026
3.76 ± 0.013
7.044 ± 0.02
2.497 ± 0.012
5.213 ± 0.015
5.409 ± 0.022
9.028 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.189 ± 0.009
3.842 ± 0.013
6.185 ± 0.027
3.707 ± 0.017
6.051 ± 0.017
8.271 ± 0.025
6.039 ± 0.017
6.094 ± 0.016
1.396 ± 0.008
2.775 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here