Myroides indicus
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2689 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R7ETI3|A0A4R7ETI3_9FLAO Cell division protein FtsA OS=Myroides indicus OX=1323422 GN=ftsA PE=3 SV=1
MM1 pKa = 7.9 KK2 pKa = 10.18 YY3 pKa = 10.7 FKK5 pKa = 10.32 FLSVFALFGALFLTSCSTDD24 pKa = 3.75 DD25 pKa = 5.75 KK26 pKa = 10.97 DD27 pKa = 4.55 TQPTDD32 pKa = 3.29 QQIEE36 pKa = 4.37 NKK38 pKa = 10.41 SFFHH42 pKa = 7.68 DD43 pKa = 4.33 LFHH46 pKa = 8.06 PDD48 pKa = 3.25 GLQSNSRR55 pKa = 11.84 YY56 pKa = 5.3 TTFAAGDD63 pKa = 3.6 YY64 pKa = 10.34 AGTYY68 pKa = 8.2 TGTYY72 pKa = 9.42 VINSMLAGSGPGDD85 pKa = 3.6 YY86 pKa = 10.97 DD87 pKa = 3.73 DD88 pKa = 6.35 VEE90 pKa = 4.9 DD91 pKa = 4.38 KK92 pKa = 11.26 SITITVNEE100 pKa = 4.19 TAQTIDD106 pKa = 4.66 LEE108 pKa = 4.23 IEE110 pKa = 4.05 PFKK113 pKa = 10.85 VGSMPANINIEE124 pKa = 4.39 MYY126 pKa = 10.3 SIPYY130 pKa = 8.81 TEE132 pKa = 4.54 TGNTVHH138 pKa = 6.71 FQGTEE143 pKa = 3.91 TNGFSVMGVYY153 pKa = 10.46 SDD155 pKa = 3.88 IDD157 pKa = 3.5 VDD159 pKa = 3.95 GTITKK164 pKa = 10.47 SGTTYY169 pKa = 10.8 TMALNFLANGSWGSMIINADD189 pKa = 3.08 VDD191 pKa = 4.26 FTGTKK196 pKa = 9.97
Molecular weight: 21.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 4.012
IPC_protein 3.999
Toseland 3.783
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.935
Rodwell 3.834
Grimsley 3.694
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.368
Thurlkill 3.846
EMBOSS 3.948
Sillero 4.126
Patrickios 1.1
IPC_peptide 3.986
IPC2_peptide 4.101
IPC2.peptide.svr19 3.99
Protein with the highest isoelectric point:
>tr|A0A4V3E842|A0A4V3E842_9FLAO RHS repeat-associated protein OS=Myroides indicus OX=1323422 GN=C8P70_11740 PE=4 SV=1
MM1 pKa = 7.36 LRR3 pKa = 11.84 HH4 pKa = 5.93 EE5 pKa = 4.48 GRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 TFFHH13 pKa = 6.02 NLKK16 pKa = 9.9 LASILSTVAGIVNIVGVLSVKK37 pKa = 10.45 VLTTNVTGHH46 pKa = 6.39 FAFFSEE52 pKa = 4.16 EE53 pKa = 4.46 LFFKK57 pKa = 10.45 DD58 pKa = 3.54 YY59 pKa = 11.42 SLAGLFLIYY68 pKa = 10.18 IFSFLGGAFISSLLMDD84 pKa = 3.99 FSKK87 pKa = 10.9 RR88 pKa = 11.84 IRR90 pKa = 11.84 FRR92 pKa = 11.84 STYY95 pKa = 8.86 ILPVFLEE102 pKa = 3.79 ITLFILVMVCSLFYY116 pKa = 10.63 TGVDD120 pKa = 3.29 QAIASMLLFAMGLQNALVTRR140 pKa = 11.84 VSQSVVRR147 pKa = 11.84 TTHH150 pKa = 5.1 LTGLFTDD157 pKa = 4.66 LGIEE161 pKa = 4.21 LSFLFFKK168 pKa = 10.2 QHH170 pKa = 4.35 YY171 pKa = 8.36 KK172 pKa = 10.29 KK173 pKa = 10.43 RR174 pKa = 11.84 SLRR177 pKa = 11.84 RR178 pKa = 11.84 SIGLKK183 pKa = 9.72 ISIIGGFFLGCILGGFVYY201 pKa = 10.08 QQLEE205 pKa = 4.45 IYY207 pKa = 9.77 TLFIPVALLLFALWYY222 pKa = 10.16 DD223 pKa = 3.55 RR224 pKa = 11.84 LIFKK228 pKa = 9.92 YY229 pKa = 10.69 LILRR233 pKa = 11.84 RR234 pKa = 11.84 KK235 pKa = 10.2 LIAQRR240 pKa = 11.84 RR241 pKa = 11.84 NILKK245 pKa = 10.29 LRR247 pKa = 3.89
Molecular weight: 28.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.897
IPC_protein 10.613
Toseland 10.57
ProMoST 10.394
Dawson 10.716
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.994
Grimsley 10.789
Solomon 10.789
Lehninger 10.745
Nozaki 10.555
DTASelect 10.423
Thurlkill 10.599
EMBOSS 10.965
Sillero 10.643
Patrickios 10.687
IPC_peptide 10.789
IPC2_peptide 9.37
IPC2.peptide.svr19 8.465
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2689
0
2689
898407
26
3563
334.1
37.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.078 ± 0.05
0.815 ± 0.02
5.362 ± 0.035
6.873 ± 0.056
5.203 ± 0.038
6.245 ± 0.047
1.712 ± 0.021
8.242 ± 0.045
7.898 ± 0.057
9.236 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.231 ± 0.026
6.092 ± 0.044
3.248 ± 0.029
3.687 ± 0.031
3.355 ± 0.033
6.413 ± 0.048
5.671 ± 0.055
6.152 ± 0.04
1.043 ± 0.02
4.446 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here