Radiobacillus deserti

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Radiobacillus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KGP5|A0A516KGP5_9BACI Uncharacterized protein OS=Radiobacillus deserti OX=2594883 GN=FN924_10485 PE=4 SV=1
MM1 pKa = 7.65AKK3 pKa = 10.18FAIVDD8 pKa = 3.53KK9 pKa = 9.39DD10 pKa = 3.5TCIACGACGATASSIFQYY28 pKa = 10.97DD29 pKa = 3.95DD30 pKa = 3.11EE31 pKa = 5.63GLAYY35 pKa = 10.49VAVDD39 pKa = 3.82EE40 pKa = 4.75NKK42 pKa = 10.06GVCPIPEE49 pKa = 4.08DD50 pKa = 3.45WEE52 pKa = 4.29EE53 pKa = 4.04DD54 pKa = 3.67VEE56 pKa = 5.47DD57 pKa = 5.04AFLGCPSDD65 pKa = 4.13SIKK68 pKa = 10.73MLDD71 pKa = 3.57KK72 pKa = 10.76PFNGDD77 pKa = 3.52PLKK80 pKa = 11.09YY81 pKa = 10.21DD82 pKa = 3.29

Molecular weight: 8.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KKX9|A0A516KKX9_9BACI MerR family transcriptional regulator OS=Radiobacillus deserti OX=2594883 GN=FN924_01590 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.02KK14 pKa = 8.47VHH16 pKa = 5.57GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 10.29NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3501

0

3501

973665

24

1516

278.1

31.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.589 ± 0.042

0.635 ± 0.01

5.256 ± 0.032

7.634 ± 0.049

4.621 ± 0.041

6.667 ± 0.04

2.18 ± 0.02

7.808 ± 0.039

6.705 ± 0.038

9.764 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.02

4.294 ± 0.033

3.603 ± 0.023

4.019 ± 0.033

3.995 ± 0.031

6.186 ± 0.036

5.452 ± 0.032

7.167 ± 0.03

1.102 ± 0.017

3.602 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski