Amino acid dipepetide frequency for Radiobacillus deserti

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.824AlaAla: 4.824 ± 0.087
0.552AlaCys: 0.552 ± 0.023
3.093AlaAsp: 3.093 ± 0.059
4.263AlaGlu: 4.263 ± 0.068
3.302AlaPhe: 3.302 ± 0.065
4.777AlaGly: 4.777 ± 0.077
1.237AlaHis: 1.237 ± 0.038
5.86AlaIle: 5.86 ± 0.086
4.423AlaLys: 4.423 ± 0.057
6.777AlaLeu: 6.777 ± 0.099
1.907AlaMet: 1.907 ± 0.048
2.681AlaAsn: 2.681 ± 0.06
1.949AlaPro: 1.949 ± 0.046
2.214AlaGln: 2.214 ± 0.055
2.497AlaArg: 2.497 ± 0.054
4.164AlaSer: 4.164 ± 0.071
3.555AlaThr: 3.555 ± 0.069
4.805AlaVal: 4.805 ± 0.078
0.665AlaTrp: 0.665 ± 0.031
2.35AlaTyr: 2.35 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.35CysAla: 0.35 ± 0.018
0.076CysCys: 0.076 ± 0.009
0.339CysAsp: 0.339 ± 0.021
0.39CysGlu: 0.39 ± 0.021
0.305CysPhe: 0.305 ± 0.017
0.581CysGly: 0.581 ± 0.028
0.18CysHis: 0.18 ± 0.014
0.462CysIle: 0.462 ± 0.025
0.336CysLys: 0.336 ± 0.022
0.645CysLeu: 0.645 ± 0.03
0.166CysMet: 0.166 ± 0.013
0.231CysAsn: 0.231 ± 0.017
0.318CysPro: 0.318 ± 0.02
0.221CysGln: 0.221 ± 0.013
0.24CysArg: 0.24 ± 0.014
0.447CysSer: 0.447 ± 0.021
0.354CysThr: 0.354 ± 0.019
0.396CysVal: 0.396 ± 0.02
0.055CysTrp: 0.055 ± 0.007
0.258CysTyr: 0.258 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.195AspAla: 3.195 ± 0.06
0.354AspCys: 0.354 ± 0.02
2.39AspAsp: 2.39 ± 0.057
4.204AspGlu: 4.204 ± 0.08
2.565AspPhe: 2.565 ± 0.062
3.523AspGly: 3.523 ± 0.063
1.206AspHis: 1.206 ± 0.037
4.416AspIle: 4.416 ± 0.068
3.266AspLys: 3.266 ± 0.064
4.976AspLeu: 4.976 ± 0.07
1.402AspMet: 1.402 ± 0.042
1.912AspAsn: 1.912 ± 0.038
2.066AspPro: 2.066 ± 0.049
2.211AspGln: 2.211 ± 0.043
2.277AspArg: 2.277 ± 0.053
2.877AspSer: 2.877 ± 0.057
2.561AspThr: 2.561 ± 0.043
4.085AspVal: 4.085 ± 0.075
0.781AspTrp: 0.781 ± 0.029
2.288AspTyr: 2.288 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
5.539GluAla: 5.539 ± 0.075
0.31GluCys: 0.31 ± 0.018
4.277GluAsp: 4.277 ± 0.076
7.55GluGlu: 7.55 ± 0.101
2.458GluPhe: 2.458 ± 0.049
4.294GluGly: 4.294 ± 0.073
1.697GluHis: 1.697 ± 0.042
5.409GluIle: 5.409 ± 0.087
6.452GluLys: 6.452 ± 0.103
7.193GluLeu: 7.193 ± 0.101
2.248GluMet: 2.248 ± 0.052
3.58GluAsn: 3.58 ± 0.072
2.092GluPro: 2.092 ± 0.054
3.789GluGln: 3.789 ± 0.066
3.401GluArg: 3.401 ± 0.059
3.747GluSer: 3.747 ± 0.066
3.896GluThr: 3.896 ± 0.073
5.301GluVal: 5.301 ± 0.085
0.863GluTrp: 0.863 ± 0.03
2.243GluTyr: 2.243 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
2.804PheAla: 2.804 ± 0.053
0.316PheCys: 0.316 ± 0.016
2.341PheAsp: 2.341 ± 0.054
2.885PheGlu: 2.885 ± 0.057
2.422PhePhe: 2.422 ± 0.06
3.212PheGly: 3.212 ± 0.056
1.136PheHis: 1.136 ± 0.036
3.906PheIle: 3.906 ± 0.075
2.309PheLys: 2.309 ± 0.047
4.918PheLeu: 4.918 ± 0.093
1.219PheMet: 1.219 ± 0.036
1.817PheAsn: 1.817 ± 0.044
1.713PhePro: 1.713 ± 0.045
1.889PheGln: 1.889 ± 0.043
1.673PheArg: 1.673 ± 0.042
3.359PheSer: 3.359 ± 0.07
2.535PheThr: 2.535 ± 0.056
3.452PheVal: 3.452 ± 0.069
0.499PheTrp: 0.499 ± 0.024
1.807PheTyr: 1.807 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
4.449GlyAla: 4.449 ± 0.079
0.529GlyCys: 0.529 ± 0.028
3.21GlyAsp: 3.21 ± 0.061
4.447GlyGlu: 4.447 ± 0.077
3.417GlyPhe: 3.417 ± 0.063
4.487GlyGly: 4.487 ± 0.088
1.291GlyHis: 1.291 ± 0.032
5.807GlyIle: 5.807 ± 0.075
4.672GlyLys: 4.672 ± 0.066
6.505GlyLeu: 6.505 ± 0.091
2.021GlyMet: 2.021 ± 0.044
2.774GlyAsn: 2.774 ± 0.053
1.714GlyPro: 1.714 ± 0.035
2.156GlyGln: 2.156 ± 0.044
2.461GlyArg: 2.461 ± 0.046
3.973GlySer: 3.973 ± 0.065
3.967GlyThr: 3.967 ± 0.064
5.087GlyVal: 5.087 ± 0.078
0.844GlyTrp: 0.844 ± 0.032
2.857GlyTyr: 2.857 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.408HisAla: 1.408 ± 0.04
0.19HisCys: 0.19 ± 0.015
1.116HisAsp: 1.116 ± 0.037
1.305HisGlu: 1.305 ± 0.041
1.091HisPhe: 1.091 ± 0.031
1.463HisGly: 1.463 ± 0.039
0.71HisHis: 0.71 ± 0.028
1.672HisIle: 1.672 ± 0.039
1.095HisLys: 1.095 ± 0.033
2.21HisLeu: 2.21 ± 0.045
0.527HisMet: 0.527 ± 0.021
0.793HisAsn: 0.793 ± 0.028
1.161HisPro: 1.161 ± 0.037
0.933HisGln: 0.933 ± 0.032
0.889HisArg: 0.889 ± 0.031
1.341HisSer: 1.341 ± 0.042
1.141HisThr: 1.141 ± 0.034
1.605HisVal: 1.605 ± 0.041
0.231HisTrp: 0.231 ± 0.016
0.92HisTyr: 0.92 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.688IleAla: 5.688 ± 0.089
0.6IleCys: 0.6 ± 0.028
4.188IleAsp: 4.188 ± 0.064
5.602IleGlu: 5.602 ± 0.09
3.288IlePhe: 3.288 ± 0.064
6.166IleGly: 6.166 ± 0.089
1.913IleHis: 1.913 ± 0.046
5.654IleIle: 5.654 ± 0.099
4.432IleLys: 4.432 ± 0.077
7.378IleLeu: 7.378 ± 0.101
1.813IleMet: 1.813 ± 0.045
3.024IleAsn: 3.024 ± 0.064
3.467IlePro: 3.467 ± 0.069
3.266IleGln: 3.266 ± 0.057
3.126IleArg: 3.126 ± 0.063
5.18IleSer: 5.18 ± 0.092
4.467IleThr: 4.467 ± 0.067
5.635IleVal: 5.635 ± 0.081
0.727IleTrp: 0.727 ± 0.025
2.462IleTyr: 2.462 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
4.32LysAla: 4.32 ± 0.068
0.283LysCys: 0.283 ± 0.019
3.986LysAsp: 3.986 ± 0.06
7.206LysGlu: 7.206 ± 0.091
1.812LysPhe: 1.812 ± 0.045
4.282LysGly: 4.282 ± 0.073
1.382LysHis: 1.382 ± 0.039
4.156LysIle: 4.156 ± 0.074
5.511LysLys: 5.511 ± 0.083
5.43LysLeu: 5.43 ± 0.082
2.041LysMet: 2.041 ± 0.041
2.986LysAsn: 2.986 ± 0.059
2.216LysPro: 2.216 ± 0.05
3.608LysGln: 3.608 ± 0.061
3.419LysArg: 3.419 ± 0.067
3.46LysSer: 3.46 ± 0.059
3.423LysThr: 3.423 ± 0.064
4.661LysVal: 4.661 ± 0.074
0.867LysTrp: 0.867 ± 0.029
2.0LysTyr: 2.0 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
6.874LeuAla: 6.874 ± 0.096
0.623LeuCys: 0.623 ± 0.026
5.167LeuAsp: 5.167 ± 0.078
7.214LeuGlu: 7.214 ± 0.111
5.146LeuPhe: 5.146 ± 0.087
6.109LeuGly: 6.109 ± 0.102
2.152LeuHis: 2.152 ± 0.052
7.181LeuIle: 7.181 ± 0.116
6.205LeuLys: 6.205 ± 0.075
10.343LeuLeu: 10.343 ± 0.154
2.379LeuMet: 2.379 ± 0.049
3.931LeuAsn: 3.931 ± 0.071
3.872LeuPro: 3.872 ± 0.07
3.787LeuGln: 3.787 ± 0.064
3.64LeuArg: 3.64 ± 0.069
6.907LeuSer: 6.907 ± 0.076
5.554LeuThr: 5.554 ± 0.092
6.483LeuVal: 6.483 ± 0.082
0.905LeuTrp: 0.905 ± 0.033
3.168LeuTyr: 3.168 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
1.846MetAla: 1.846 ± 0.039
0.121MetCys: 0.121 ± 0.013
1.747MetAsp: 1.747 ± 0.042
2.289MetGlu: 2.289 ± 0.04
1.096MetPhe: 1.096 ± 0.035
1.664MetGly: 1.664 ± 0.042
0.401MetHis: 0.401 ± 0.019
2.05MetIle: 2.05 ± 0.041
2.428MetLys: 2.428 ± 0.048
2.396MetLeu: 2.396 ± 0.051
0.772MetMet: 0.772 ± 0.025
1.53MetAsn: 1.53 ± 0.046
0.924MetPro: 0.924 ± 0.033
0.888MetGln: 0.888 ± 0.026
1.001MetArg: 1.001 ± 0.037
1.662MetSer: 1.662 ± 0.045
1.544MetThr: 1.544 ± 0.04
1.847MetVal: 1.847 ± 0.048
0.213MetTrp: 0.213 ± 0.014
0.786MetTyr: 0.786 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.418AsnAla: 2.418 ± 0.049
0.25AsnCys: 0.25 ± 0.014
2.182AsnAsp: 2.182 ± 0.05
3.22AsnGlu: 3.22 ± 0.059
1.521AsnPhe: 1.521 ± 0.037
3.26AsnGly: 3.26 ± 0.071
1.149AsnHis: 1.149 ± 0.041
3.234AsnIle: 3.234 ± 0.059
2.844AsnLys: 2.844 ± 0.06
3.687AsnLeu: 3.687 ± 0.056
1.14AsnMet: 1.14 ± 0.035
1.873AsnAsn: 1.873 ± 0.048
2.168AsnPro: 2.168 ± 0.051
2.308AsnGln: 2.308 ± 0.055
2.128AsnArg: 2.128 ± 0.046
2.234AsnSer: 2.234 ± 0.058
2.115AsnThr: 2.115 ± 0.048
3.138AsnVal: 3.138 ± 0.06
0.542AsnTrp: 0.542 ± 0.024
1.531AsnTyr: 1.531 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
2.099ProAla: 2.099 ± 0.053
0.188ProCys: 0.188 ± 0.013
2.087ProAsp: 2.087 ± 0.048
3.041ProGlu: 3.041 ± 0.06
2.047ProPhe: 2.047 ± 0.053
2.233ProGly: 2.233 ± 0.057
0.761ProHis: 0.761 ± 0.029
3.049ProIle: 3.049 ± 0.059
2.151ProLys: 2.151 ± 0.05
3.428ProLeu: 3.428 ± 0.057
0.827ProMet: 0.827 ± 0.027
1.79ProAsn: 1.79 ± 0.043
0.979ProPro: 0.979 ± 0.037
1.054ProGln: 1.054 ± 0.03
1.054ProArg: 1.054 ± 0.038
2.52ProSer: 2.52 ± 0.056
2.132ProThr: 2.132 ± 0.039
2.755ProVal: 2.755 ± 0.064
0.384ProTrp: 0.384 ± 0.024
1.454ProTyr: 1.454 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
3.053GlnAla: 3.053 ± 0.052
0.163GlnCys: 0.163 ± 0.013
1.973GlnAsp: 1.973 ± 0.044
3.27GlnGlu: 3.27 ± 0.066
1.56GlnPhe: 1.56 ± 0.042
2.173GlnGly: 2.173 ± 0.046
0.917GlnHis: 0.917 ± 0.034
2.837GlnIle: 2.837 ± 0.055
2.86GlnLys: 2.86 ± 0.059
4.25GlnLeu: 4.25 ± 0.067
1.112GlnMet: 1.112 ± 0.036
1.666GlnAsn: 1.666 ± 0.038
1.313GlnPro: 1.313 ± 0.036
2.032GlnGln: 2.032 ± 0.054
1.591GlnArg: 1.591 ± 0.043
2.489GlnSer: 2.489 ± 0.053
2.181GlnThr: 2.181 ± 0.047
2.825GlnVal: 2.825 ± 0.058
0.488GlnTrp: 0.488 ± 0.021
1.44GlnTyr: 1.44 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.322ArgAla: 2.322 ± 0.052
0.212ArgCys: 0.212 ± 0.015
2.118ArgAsp: 2.118 ± 0.054
3.091ArgGlu: 3.091 ± 0.058
1.933ArgPhe: 1.933 ± 0.042
2.25ArgGly: 2.25 ± 0.048
0.764ArgHis: 0.764 ± 0.03
3.134ArgIle: 3.134 ± 0.057
3.271ArgLys: 3.271 ± 0.065
3.938ArgLeu: 3.938 ± 0.068
1.293ArgMet: 1.293 ± 0.039
1.99ArgAsn: 1.99 ± 0.035
1.266ArgPro: 1.266 ± 0.039
1.481ArgGln: 1.481 ± 0.04
1.84ArgArg: 1.84 ± 0.052
2.227ArgSer: 2.227 ± 0.046
2.168ArgThr: 2.168 ± 0.048
2.637ArgVal: 2.637 ± 0.057
0.418ArgTrp: 0.418 ± 0.022
1.599ArgTyr: 1.599 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
3.454SerAla: 3.454 ± 0.06
0.341SerCys: 0.341 ± 0.018
3.111SerAsp: 3.111 ± 0.061
4.046SerGlu: 4.046 ± 0.066
3.663SerPhe: 3.663 ± 0.068
4.189SerGly: 4.189 ± 0.067
1.28SerHis: 1.28 ± 0.041
5.643SerIle: 5.643 ± 0.086
3.882SerLys: 3.882 ± 0.06
6.324SerLeu: 6.324 ± 0.086
1.736SerMet: 1.736 ± 0.043
2.785SerAsn: 2.785 ± 0.052
2.163SerPro: 2.163 ± 0.046
2.012SerGln: 2.012 ± 0.051
2.207SerArg: 2.207 ± 0.042
4.227SerSer: 4.227 ± 0.092
3.32SerThr: 3.32 ± 0.068
4.34SerVal: 4.34 ± 0.068
0.63SerTrp: 0.63 ± 0.025
2.508SerTyr: 2.508 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
3.571ThrAla: 3.571 ± 0.079
0.348ThrCys: 0.348 ± 0.018
2.759ThrAsp: 2.759 ± 0.058
3.607ThrGlu: 3.607 ± 0.072
2.813ThrPhe: 2.813 ± 0.052
3.922ThrGly: 3.922 ± 0.057
1.039ThrHis: 1.039 ± 0.032
4.831ThrIle: 4.831 ± 0.075
3.426ThrLys: 3.426 ± 0.056
5.232ThrLeu: 5.232 ± 0.08
1.337ThrMet: 1.337 ± 0.035
2.561ThrAsn: 2.561 ± 0.064
2.191ThrPro: 2.191 ± 0.044
1.478ThrGln: 1.478 ± 0.038
1.848ThrArg: 1.848 ± 0.044
3.504ThrSer: 3.504 ± 0.058
3.075ThrThr: 3.075 ± 0.062
4.165ThrVal: 4.165 ± 0.065
0.621ThrTrp: 0.621 ± 0.026
2.192ThrTyr: 2.192 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
4.976ValAla: 4.976 ± 0.079
0.562ValCys: 0.562 ± 0.026
3.902ValAsp: 3.902 ± 0.059
5.344ValGlu: 5.344 ± 0.092
3.286ValPhe: 3.286 ± 0.063
4.789ValGly: 4.789 ± 0.08
1.488ValHis: 1.488 ± 0.04
5.551ValIle: 5.551 ± 0.086
4.483ValLys: 4.483 ± 0.075
7.253ValLeu: 7.253 ± 0.087
1.935ValMet: 1.935 ± 0.052
2.917ValAsn: 2.917 ± 0.054
2.625ValPro: 2.625 ± 0.057
2.697ValGln: 2.697 ± 0.056
2.666ValArg: 2.666 ± 0.058
4.781ValSer: 4.781 ± 0.084
4.217ValThr: 4.217 ± 0.063
5.233ValVal: 5.233 ± 0.09
0.679ValTrp: 0.679 ± 0.03
2.284ValTyr: 2.284 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.604TrpAla: 0.604 ± 0.024
0.066TrpCys: 0.066 ± 0.009
0.591TrpAsp: 0.591 ± 0.026
0.712TrpGlu: 0.712 ± 0.026
0.604TrpPhe: 0.604 ± 0.031
0.703TrpGly: 0.703 ± 0.026
0.206TrpHis: 0.206 ± 0.014
0.924TrpIle: 0.924 ± 0.033
0.861TrpLys: 0.861 ± 0.029
1.212TrpLeu: 1.212 ± 0.038
0.398TrpMet: 0.398 ± 0.022
0.597TrpAsn: 0.597 ± 0.028
0.272TrpPro: 0.272 ± 0.021
0.388TrpGln: 0.388 ± 0.02
0.403TrpArg: 0.403 ± 0.019
0.642TrpSer: 0.642 ± 0.029
0.553TrpThr: 0.553 ± 0.022
0.743TrpVal: 0.743 ± 0.029
0.162TrpTrp: 0.162 ± 0.013
0.384TrpTyr: 0.384 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.1TyrAla: 2.1 ± 0.046
0.268TyrCys: 0.268 ± 0.016
1.979TyrAsp: 1.979 ± 0.053
2.652TyrGlu: 2.652 ± 0.059
1.884TyrPhe: 1.884 ± 0.044
2.592TyrGly: 2.592 ± 0.051
0.893TyrHis: 0.893 ± 0.031
2.299TyrIle: 2.299 ± 0.049
2.141TyrLys: 2.141 ± 0.051
3.543TyrLeu: 3.543 ± 0.058
0.929TyrMet: 0.929 ± 0.032
1.504TyrAsn: 1.504 ± 0.042
1.562TyrPro: 1.562 ± 0.037
1.784TyrGln: 1.784 ± 0.043
1.592TyrArg: 1.592 ± 0.045
2.121TyrSer: 2.121 ± 0.047
1.762TyrThr: 1.762 ± 0.044
2.476TyrVal: 2.476 ± 0.05
0.45TyrTrp: 0.45 ± 0.02
1.486TyrTyr: 1.486 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3501 proteins (973665 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski