Streptomyces asterosporus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6358 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514JTB7|A0A514JTB7_9ACTN FmdB family transcriptional regulator OS=Streptomyces asterosporus OX=285570 GN=CD934_19405 PE=4 SV=1
MM1 pKa = 7.52NSRR4 pKa = 11.84GNALKK9 pKa = 9.26LTAVSALVVLTLTGFSTGRR28 pKa = 11.84GGSSSGGGDD37 pKa = 3.22GSGGGGCSSSSQNHH51 pKa = 6.75DD52 pKa = 3.19SSGSGGSHH60 pKa = 6.73SDD62 pKa = 3.31YY63 pKa = 11.43DD64 pKa = 4.16DD65 pKa = 6.34DD66 pKa = 6.67YY67 pKa = 11.98DD68 pKa = 3.99DD69 pKa = 5.27TYY71 pKa = 12.03DD72 pKa = 4.26DD73 pKa = 4.64TYY75 pKa = 11.84DD76 pKa = 3.53DD77 pKa = 4.26TGSGGADD84 pKa = 2.92VDD86 pKa = 4.15ASPAEE91 pKa = 3.79QDD93 pKa = 3.25ATVEE97 pKa = 4.22LVDD100 pKa = 4.51CASPTAPYY108 pKa = 9.29ATVEE112 pKa = 4.22VTNPNAASGSFTVTVTFRR130 pKa = 11.84DD131 pKa = 3.43ASGAEE136 pKa = 3.92VTAPAKK142 pKa = 10.15DD143 pKa = 3.41VHH145 pKa = 7.57VPGNDD150 pKa = 2.89TVTARR155 pKa = 11.84VEE157 pKa = 4.13IDD159 pKa = 3.35DD160 pKa = 3.79SAAAGRR166 pKa = 11.84VDD168 pKa = 5.34DD169 pKa = 5.33CDD171 pKa = 4.21PDD173 pKa = 4.53PYY175 pKa = 11.23APPAVV180 pKa = 3.56

Molecular weight: 17.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514JSW4|A0A514JSW4_9ACTN Protein translocase subunit SecE OS=Streptomyces asterosporus OX=285570 GN=secE PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6358

0

6358

2202986

18

13220

346.5

37.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.685 ± 0.053

0.767 ± 0.01

6.265 ± 0.029

5.784 ± 0.032

2.678 ± 0.019

9.513 ± 0.033

2.335 ± 0.019

2.876 ± 0.025

1.979 ± 0.027

10.27 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.656 ± 0.014

1.705 ± 0.019

6.241 ± 0.033

2.624 ± 0.018

8.404 ± 0.042

4.844 ± 0.025

6.225 ± 0.029

8.619 ± 0.029

1.489 ± 0.013

2.043 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski