Brevibacterium sp. 5221

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Brevibacteriaceae; Brevibacterium

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2629 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N9H7Y7|A0A6N9H7Y7_9MICO Exopolyphosphatase OS=Brevibacterium sp. 5221 OX=2695267 GN=GSY69_08495 PE=4 SV=1
MM1 pKa = 7.03EE2 pKa = 4.38TVGDD6 pKa = 4.0EE7 pKa = 4.42SAGGGAFAGDD17 pKa = 3.92GVFDD21 pKa = 4.15GLVEE25 pKa = 3.99EE26 pKa = 5.59AMAALPAEE34 pKa = 4.36VTEE37 pKa = 4.49RR38 pKa = 11.84LDD40 pKa = 3.34NVAFFIADD48 pKa = 4.01DD49 pKa = 4.65PPPEE53 pKa = 4.6APSILGLYY61 pKa = 9.58EE62 pKa = 5.04GIALTEE68 pKa = 3.68RR69 pKa = 11.84DD70 pKa = 3.41AAYY73 pKa = 10.44GFVPPDD79 pKa = 3.9TITLFKK85 pKa = 11.03NPLIACARR93 pKa = 11.84DD94 pKa = 3.34EE95 pKa = 4.63DD96 pKa = 4.06EE97 pKa = 6.14LYY99 pKa = 9.81EE100 pKa = 4.0QIYY103 pKa = 7.69ITIVHH108 pKa = 6.31EE109 pKa = 4.55VAHH112 pKa = 5.81HH113 pKa = 6.41HH114 pKa = 7.08GIDD117 pKa = 3.79DD118 pKa = 4.96DD119 pKa = 4.26EE120 pKa = 4.41LHH122 pKa = 6.1EE123 pKa = 5.11LGWGG127 pKa = 3.46

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N9H7Z9|A0A6N9H7Z9_9MICO Helix-turn-helix domain-containing protein OS=Brevibacterium sp. 5221 OX=2695267 GN=GSY69_08625 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.46THH17 pKa = 5.11GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.99GRR40 pKa = 11.84TNISAA45 pKa = 3.72

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2629

0

2629

871902

32

1773

331.6

35.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.372 ± 0.093

0.667 ± 0.013

5.827 ± 0.04

5.752 ± 0.049

2.966 ± 0.026

9.687 ± 0.048

2.011 ± 0.022

4.0 ± 0.035

2.154 ± 0.042

9.734 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.849 ± 0.019

1.752 ± 0.028

5.807 ± 0.036

3.006 ± 0.029

7.449 ± 0.055

5.394 ± 0.037

5.432 ± 0.036

7.94 ± 0.042

1.329 ± 0.017

1.87 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski