Amino acid dipepetide frequency for Brevibacterium sp. 5221

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	27.056AlaAla: 27.056 ± 0.3	1.062AlaCys: 1.062 ± 0.035	9.514AlaAsp: 9.514 ± 0.127	9.289AlaGlu: 9.289 ± 0.134	3.861AlaPhe: 3.861 ± 0.078	15.986AlaGly: 15.986 ± 0.168	3.3AlaHis: 3.3 ± 0.064	5.13AlaIle: 5.13 ± 0.092	3.348AlaLys: 3.348 ± 0.101	15.693AlaLeu: 15.693 ± 0.186	2.757AlaMet: 2.757 ± 0.058	2.345AlaAsn: 2.345 ± 0.057	8.409AlaPro: 8.409 ± 0.145	6.404AlaGln: 6.404 ± 0.104	10.897AlaArg: 10.897 ± 0.16	6.773AlaSer: 6.773 ± 0.089	6.094AlaThr: 6.094 ± 0.077	11.471AlaVal: 11.471 ± 0.141	1.859AlaTrp: 1.859 ± 0.047	2.474AlaTyr: 2.474 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	1.165CysAla: 1.165 ± 0.043	0.073CysCys: 0.073 ± 0.01	0.333CysAsp: 0.333 ± 0.021	0.382CysGlu: 0.382 ± 0.021	0.209CysPhe: 0.209 ± 0.014	0.745CysGly: 0.745 ± 0.032	0.126CysHis: 0.126 ± 0.014	0.229CysIle: 0.229 ± 0.014	0.1CysLys: 0.1 ± 0.01	0.6CysLeu: 0.6 ± 0.026	0.106CysMet: 0.106 ± 0.009	0.111CysAsn: 0.111 ± 0.01	0.393CysPro: 0.393 ± 0.027	0.138CysGln: 0.138 ± 0.013	0.436CysArg: 0.436 ± 0.026	0.372CysSer: 0.372 ± 0.02	0.412CysThr: 0.412 ± 0.023	0.506CysVal: 0.506 ± 0.023	0.102CysTrp: 0.102 ± 0.012	0.131CysTyr: 0.131 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.478AspAla: 8.478 ± 0.117	0.315AspCys: 0.315 ± 0.02	3.629AspAsp: 3.629 ± 0.081	4.085AspGlu: 4.085 ± 0.075	1.938AspPhe: 1.938 ± 0.053	5.538AspGly: 5.538 ± 0.1	1.163AspHis: 1.163 ± 0.043	2.256AspIle: 2.256 ± 0.061	1.061AspLys: 1.061 ± 0.042	5.403AspLeu: 5.403 ± 0.092	0.967AspMet: 0.967 ± 0.036	0.88AspAsn: 0.88 ± 0.037	4.345AspPro: 4.345 ± 0.067	1.372AspGln: 1.372 ± 0.041	4.221AspArg: 4.221 ± 0.074	3.079AspSer: 3.079 ± 0.077	2.684AspThr: 2.684 ± 0.059	4.529AspVal: 4.529 ± 0.081	0.912AspTrp: 0.912 ± 0.032	1.418AspTyr: 1.418 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	8.633GluAla: 8.633 ± 0.135	0.302GluCys: 0.302 ± 0.018	3.146GluAsp: 3.146 ± 0.07	3.234GluGlu: 3.234 ± 0.076	1.696GluPhe: 1.696 ± 0.047	4.23GluGly: 4.23 ± 0.07	1.594GluHis: 1.594 ± 0.049	2.212GluIle: 2.212 ± 0.056	1.257GluLys: 1.257 ± 0.053	6.469GluLeu: 6.469 ± 0.103	0.843GluMet: 0.843 ± 0.032	0.928GluAsn: 0.928 ± 0.036	3.503GluPro: 3.503 ± 0.074	2.111GluGln: 2.111 ± 0.048	5.496GluArg: 5.496 ± 0.105	2.803GluSer: 2.803 ± 0.064	2.547GluThr: 2.547 ± 0.056	4.704GluVal: 4.704 ± 0.088	0.74GluTrp: 0.74 ± 0.025	1.077GluTyr: 1.077 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	4.791PheAla: 4.791 ± 0.088	0.234PheCys: 0.234 ± 0.015	2.404PheAsp: 2.404 ± 0.055	1.693PheGlu: 1.693 ± 0.051	1.037PhePhe: 1.037 ± 0.039	3.397PheGly: 3.397 ± 0.073	0.544PheHis: 0.544 ± 0.026	1.082PheIle: 1.082 ± 0.041	0.517PheLys: 0.517 ± 0.026	2.415PheLeu: 2.415 ± 0.058	0.529PheMet: 0.529 ± 0.024	0.642PheAsn: 0.642 ± 0.029	1.346PhePro: 1.346 ± 0.038	0.601PheGln: 0.601 ± 0.027	1.51PheArg: 1.51 ± 0.046	1.738PheSer: 1.738 ± 0.044	1.828PheThr: 1.828 ± 0.051	2.411PheVal: 2.411 ± 0.064	0.356PheTrp: 0.356 ± 0.023	0.586PheTyr: 0.586 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	13.763GlyAla: 13.763 ± 0.159	0.726GlyCys: 0.726 ± 0.03	4.809GlyAsp: 4.809 ± 0.085	5.505GlyGlu: 5.505 ± 0.087	3.301GlyPhe: 3.301 ± 0.066	8.868GlyGly: 8.868 ± 0.163	1.917GlyHis: 1.917 ± 0.047	4.385GlyIle: 4.385 ± 0.078	2.355GlyLys: 2.355 ± 0.068	9.509GlyLeu: 9.509 ± 0.127	2.201GlyMet: 2.201 ± 0.05	1.528GlyAsn: 1.528 ± 0.04	4.903GlyPro: 4.903 ± 0.099	3.112GlyGln: 3.112 ± 0.079	7.242GlyArg: 7.242 ± 0.099	6.135GlySer: 6.135 ± 0.122	6.033GlyThr: 6.033 ± 0.092	6.806GlyVal: 6.806 ± 0.101	1.549GlyTrp: 1.549 ± 0.045	2.225GlyTyr: 2.225 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	2.683HisAla: 2.683 ± 0.06	0.154HisCys: 0.154 ± 0.012	1.147HisAsp: 1.147 ± 0.043	1.092HisGlu: 1.092 ± 0.034	0.529HisPhe: 0.529 ± 0.025	2.129HisGly: 2.129 ± 0.053	0.494HisHis: 0.494 ± 0.023	0.74HisIle: 0.74 ± 0.031	0.303HisLys: 0.303 ± 0.017	1.827HisLeu: 1.827 ± 0.044	0.357HisMet: 0.357 ± 0.02	0.354HisAsn: 0.354 ± 0.019	1.632HisPro: 1.632 ± 0.039	0.455HisGln: 0.455 ± 0.024	1.739HisArg: 1.739 ± 0.046	1.158HisSer: 1.158 ± 0.036	1.153HisThr: 1.153 ± 0.034	1.474HisVal: 1.474 ± 0.043	0.282HisTrp: 0.282 ± 0.02	0.413HisTyr: 0.413 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	6.825IleAla: 6.825 ± 0.103	0.304IleCys: 0.304 ± 0.018	2.961IleAsp: 2.961 ± 0.062	2.548IleGlu: 2.548 ± 0.064	0.944IlePhe: 0.944 ± 0.041	4.077IleGly: 4.077 ± 0.077	0.689IleHis: 0.689 ± 0.03	1.684IleIle: 1.684 ± 0.053	0.749IleLys: 0.749 ± 0.036	3.049IleLeu: 3.049 ± 0.075	0.703IleMet: 0.703 ± 0.028	0.848IleAsn: 0.848 ± 0.034	2.029IlePro: 2.029 ± 0.044	0.743IleGln: 0.743 ± 0.032	2.394IleArg: 2.394 ± 0.051	1.978IleSer: 1.978 ± 0.046	2.306IleThr: 2.306 ± 0.047	4.22IleVal: 4.22 ± 0.087	0.33IleTrp: 0.33 ± 0.019	0.619IleTyr: 0.619 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	2.623LysAla: 2.623 ± 0.076	0.085LysCys: 0.085 ± 0.009	1.398LysAsp: 1.398 ± 0.054	1.057LysGlu: 1.057 ± 0.043	0.516LysPhe: 0.516 ± 0.025	1.722LysGly: 1.722 ± 0.055	0.415LysHis: 0.415 ± 0.019	0.872LysIle: 0.872 ± 0.033	1.1LysLys: 1.1 ± 0.064	1.919LysLeu: 1.919 ± 0.065	0.416LysMet: 0.416 ± 0.022	0.61LysAsn: 0.61 ± 0.03	1.332LysPro: 1.332 ± 0.053	0.757LysGln: 0.757 ± 0.035	1.692LysArg: 1.692 ± 0.051	1.305LysSer: 1.305 ± 0.052	1.371LysThr: 1.371 ± 0.051	1.619LysVal: 1.619 ± 0.06	0.242LysTrp: 0.242 ± 0.02	0.487LysTyr: 0.487 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	15.56LeuAla: 15.56 ± 0.19	0.716LeuCys: 0.716 ± 0.033	6.022LeuAsp: 6.022 ± 0.094	4.892LeuGlu: 4.892 ± 0.101	2.722LeuPhe: 2.722 ± 0.067	9.437LeuGly: 9.437 ± 0.127	1.736LeuHis: 1.736 ± 0.047	4.19LeuIle: 4.19 ± 0.083	1.77LeuLys: 1.77 ± 0.058	9.427LeuLeu: 9.427 ± 0.157	1.71LeuMet: 1.71 ± 0.051	1.703LeuAsn: 1.703 ± 0.046	5.569LeuPro: 5.569 ± 0.093	2.086LeuGln: 2.086 ± 0.054	7.479LeuArg: 7.479 ± 0.116	5.556LeuSer: 5.556 ± 0.087	5.995LeuThr: 5.995 ± 0.08	8.19LeuVal: 8.19 ± 0.113	1.137LeuTrp: 1.137 ± 0.04	1.445LeuTyr: 1.445 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.429MetAla: 2.429 ± 0.056	0.133MetCys: 0.133 ± 0.013	0.938MetAsp: 0.938 ± 0.033	0.807MetGlu: 0.807 ± 0.034	0.529MetPhe: 0.529 ± 0.023	1.67MetGly: 1.67 ± 0.047	0.372MetHis: 0.372 ± 0.021	0.755MetIle: 0.755 ± 0.027	0.505MetLys: 0.505 ± 0.025	1.843MetLeu: 1.843 ± 0.045	0.305MetMet: 0.305 ± 0.022	0.403MetAsn: 0.403 ± 0.021	1.077MetPro: 1.077 ± 0.035	0.463MetGln: 0.463 ± 0.025	1.514MetArg: 1.514 ± 0.037	1.594MetSer: 1.594 ± 0.042	1.381MetThr: 1.381 ± 0.041	1.291MetVal: 1.291 ± 0.042	0.2MetTrp: 0.2 ± 0.017	0.284MetTyr: 0.284 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.304AsnAla: 2.304 ± 0.052	0.112AsnCys: 0.112 ± 0.01	0.929AsnAsp: 0.929 ± 0.037	0.854AsnGlu: 0.854 ± 0.034	0.564AsnPhe: 0.564 ± 0.025	1.702AsnGly: 1.702 ± 0.058	0.328AsnHis: 0.328 ± 0.021	0.772AsnIle: 0.772 ± 0.031	0.408AsnLys: 0.408 ± 0.024	1.637AsnLeu: 1.637 ± 0.047	0.352AsnMet: 0.352 ± 0.021	0.419AsnAsn: 0.419 ± 0.023	1.451AsnPro: 1.451 ± 0.042	0.477AsnGln: 0.477 ± 0.027	1.301AsnArg: 1.301 ± 0.037	0.931AsnSer: 0.931 ± 0.036	1.009AsnThr: 1.009 ± 0.034	1.28AsnVal: 1.28 ± 0.041	0.271AsnTrp: 0.271 ± 0.019	0.416AsnTyr: 0.416 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	9.98ProAla: 9.98 ± 0.138	0.241ProCys: 0.241 ± 0.019	3.769ProAsp: 3.769 ± 0.075	4.12ProGlu: 4.12 ± 0.077	1.575ProPhe: 1.575 ± 0.043	6.618ProGly: 6.618 ± 0.107	1.262ProHis: 1.262 ± 0.047	1.777ProIle: 1.777 ± 0.051	1.205ProLys: 1.205 ± 0.047	4.72ProLeu: 4.72 ± 0.08	0.968ProMet: 0.968 ± 0.034	0.975ProAsn: 0.975 ± 0.037	2.652ProPro: 2.652 ± 0.079	2.09ProGln: 2.09 ± 0.052	3.711ProArg: 3.711 ± 0.074	2.912ProSer: 2.912 ± 0.066	3.118ProThr: 3.118 ± 0.072	4.665ProVal: 4.665 ± 0.07	0.737ProTrp: 0.737 ± 0.031	0.981ProTyr: 0.981 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	4.573GlnAla: 4.573 ± 0.084	0.147GlnCys: 0.147 ± 0.013	1.399GlnAsp: 1.399 ± 0.044	1.454GlnGlu: 1.454 ± 0.043	0.776GlnPhe: 0.776 ± 0.032	2.396GlnGly: 2.396 ± 0.062	0.637GlnHis: 0.637 ± 0.028	1.275GlnIle: 1.275 ± 0.038	0.654GlnLys: 0.654 ± 0.032	3.262GlnLeu: 3.262 ± 0.064	0.537GlnMet: 0.537 ± 0.023	0.512GlnAsn: 0.512 ± 0.028	2.06GlnPro: 2.06 ± 0.062	1.235GlnGln: 1.235 ± 0.049	2.787GlnArg: 2.787 ± 0.052	1.408GlnSer: 1.408 ± 0.041	1.512GlnThr: 1.512 ± 0.047	2.398GlnVal: 2.398 ± 0.053	0.477GlnTrp: 0.477 ± 0.02	0.561GlnTyr: 0.561 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	11.192ArgAla: 11.192 ± 0.175	0.451ArgCys: 0.451 ± 0.025	3.474ArgAsp: 3.474 ± 0.072	4.652ArgGlu: 4.652 ± 0.086	2.308ArgPhe: 2.308 ± 0.049	6.176ArgGly: 6.176 ± 0.1	1.281ArgHis: 1.281 ± 0.036	3.503ArgIle: 3.503 ± 0.07	1.325ArgLys: 1.325 ± 0.05	7.711ArgLeu: 7.711 ± 0.121	1.679ArgMet: 1.679 ± 0.042	1.125ArgAsn: 1.125 ± 0.039	4.364ArgPro: 4.364 ± 0.083	1.891ArgGln: 1.891 ± 0.047	6.938ArgArg: 6.938 ± 0.124	3.978ArgSer: 3.978 ± 0.069	4.528ArgThr: 4.528 ± 0.084	5.457ArgVal: 5.457 ± 0.087	1.059ArgTrp: 1.059 ± 0.037	1.403ArgTyr: 1.403 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	8.509SerAla: 8.509 ± 0.112	0.282SerCys: 0.282 ± 0.02	2.893SerAsp: 2.893 ± 0.071	2.867SerGlu: 2.867 ± 0.068	1.677SerPhe: 1.677 ± 0.049	6.117SerGly: 6.117 ± 0.112	1.054SerHis: 1.054 ± 0.033	2.001SerIle: 2.001 ± 0.054	1.226SerLys: 1.226 ± 0.049	4.817SerLeu: 4.817 ± 0.076	1.138SerMet: 1.138 ± 0.036	0.975SerAsn: 0.975 ± 0.036	3.338SerPro: 3.338 ± 0.062	1.825SerGln: 1.825 ± 0.055	3.352SerArg: 3.352 ± 0.058	3.037SerSer: 3.037 ± 0.073	3.131SerThr: 3.131 ± 0.059	3.919SerVal: 3.919 ± 0.075	0.713SerTrp: 0.713 ± 0.029	1.072SerTyr: 1.072 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	8.742ThrAla: 8.742 ± 0.112	0.319ThrCys: 0.319 ± 0.022	2.782ThrAsp: 2.782 ± 0.061	2.569ThrGlu: 2.569 ± 0.056	1.517ThrPhe: 1.517 ± 0.041	6.121ThrGly: 6.121 ± 0.093	1.015ThrHis: 1.015 ± 0.036	2.272ThrIle: 2.272 ± 0.06	1.267ThrLys: 1.267 ± 0.044	4.709ThrLeu: 4.709 ± 0.083	0.958ThrMet: 0.958 ± 0.035	0.973ThrAsn: 0.973 ± 0.038	3.281ThrPro: 3.281 ± 0.064	1.426ThrGln: 1.426 ± 0.048	3.28ThrArg: 3.28 ± 0.064	3.001ThrSer: 3.001 ± 0.065	3.093ThrThr: 3.093 ± 0.068	5.227ThrVal: 5.227 ± 0.072	0.764ThrTrp: 0.764 ± 0.033	1.006ThrTyr: 1.006 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.219ValAla: 10.219 ± 0.115	0.733ValCys: 0.733 ± 0.031	4.795ValAsp: 4.795 ± 0.091	4.659ValGlu: 4.659 ± 0.087	2.765ValPhe: 2.765 ± 0.065	6.968ValGly: 6.968 ± 0.098	1.614ValHis: 1.614 ± 0.044	3.649ValIle: 3.649 ± 0.077	1.681ValLys: 1.681 ± 0.065	8.77ValLeu: 8.77 ± 0.121	1.41ValMet: 1.41 ± 0.044	1.493ValAsn: 1.493 ± 0.042	4.731ValPro: 4.731 ± 0.078	1.882ValGln: 1.882 ± 0.04	5.939ValArg: 5.939 ± 0.098	4.442ValSer: 4.442 ± 0.071	4.318ValThr: 4.318 ± 0.067	6.923ValVal: 6.923 ± 0.122	0.989ValTrp: 0.989 ± 0.034	1.416ValTyr: 1.416 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.715TrpAla: 1.715 ± 0.052	0.122TrpCys: 0.122 ± 0.011	0.689TrpAsp: 0.689 ± 0.026	0.698TrpGlu: 0.698 ± 0.027	0.515TrpPhe: 0.515 ± 0.023	1.021TrpGly: 1.021 ± 0.04	0.305TrpHis: 0.305 ± 0.019	0.562TrpIle: 0.562 ± 0.022	0.343TrpLys: 0.343 ± 0.019	1.66TrpLeu: 1.66 ± 0.041	0.286TrpMet: 0.286 ± 0.018	0.327TrpAsn: 0.327 ± 0.019	0.665TrpPro: 0.665 ± 0.029	0.487TrpGln: 0.487 ± 0.024	0.957TrpArg: 0.957 ± 0.035	0.72TrpSer: 0.72 ± 0.029	0.757TrpThr: 0.757 ± 0.032	0.903TrpVal: 0.903 ± 0.034	0.296TrpTrp: 0.296 ± 0.019	0.266TrpTyr: 0.266 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.483TyrAla: 2.483 ± 0.055	0.158TyrCys: 0.158 ± 0.013	1.241TyrAsp: 1.241 ± 0.036	1.065TyrGlu: 1.065 ± 0.039	0.682TyrPhe: 0.682 ± 0.028	1.953TyrGly: 1.953 ± 0.058	0.268TyrHis: 0.268 ± 0.022	0.656TyrIle: 0.656 ± 0.028	0.365TyrLys: 0.365 ± 0.021	1.903TyrLeu: 1.903 ± 0.054	0.272TyrMet: 0.272 ± 0.02	0.368TyrAsn: 0.368 ± 0.018	0.994TyrPro: 0.994 ± 0.035	0.503TyrGln: 0.503 ± 0.025	1.609TyrArg: 1.609 ± 0.049	1.022TyrSer: 1.022 ± 0.038	1.053TyrThr: 1.053 ± 0.029	1.405TyrVal: 1.405 ± 0.038	0.279TyrTrp: 0.279 ± 0.018	0.415TyrTyr: 0.415 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2629 proteins (871902 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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