Acidithiobacillus phage AcaML1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4HZZ2|K4HZZ2_9CAUD Uncharacterized protein OS=Acidithiobacillus phage AcaML1 OX=1229761 GN=AcaML1_0032 PE=4 SV=1
MM1 pKa = 7.44AAISTTPQLEE11 pKa = 3.69AHH13 pKa = 6.49YY14 pKa = 10.06DD15 pKa = 3.4QFIAEE20 pKa = 4.56LTALTRR26 pKa = 11.84KK27 pKa = 10.1YY28 pKa = 10.46GVAIQSVGGVILADD42 pKa = 3.8APDD45 pKa = 3.53EE46 pKa = 4.24FRR48 pKa = 11.84NVTYY52 pKa = 10.5RR53 pKa = 11.84ADD55 pKa = 3.29ISSGDD60 pKa = 4.18LYY62 pKa = 10.79PEE64 pKa = 4.51FADD67 pKa = 3.92SS68 pKa = 3.8

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4I3X7|K4I3X7_9CAUD Uncharacterized protein OS=Acidithiobacillus phage AcaML1 OX=1229761 GN=AcaML1_0014 PE=4 SV=1
MM1 pKa = 7.17QKK3 pKa = 10.02HH4 pKa = 5.64PASVRR9 pKa = 11.84STRR12 pKa = 11.84NFYY15 pKa = 9.43PAPPEE20 pKa = 3.97GTTPVALNEE29 pKa = 4.12FEE31 pKa = 5.11LAARR35 pKa = 11.84WRR37 pKa = 11.84LSVHH41 pKa = 5.3TLRR44 pKa = 11.84RR45 pKa = 11.84WRR47 pKa = 11.84QEE49 pKa = 3.25QLGPVFCKK57 pKa = 10.36LGARR61 pKa = 11.84VTYY64 pKa = 10.36LISDD68 pKa = 3.8VEE70 pKa = 4.06AFEE73 pKa = 4.92RR74 pKa = 11.84RR75 pKa = 11.84VSRR78 pKa = 11.84YY79 pKa = 6.91STFARR84 pKa = 11.84AYY86 pKa = 8.62QQ87 pKa = 3.36

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

18636

63

1051

262.5

28.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.809 ± 0.498

0.778 ± 0.102

5.768 ± 0.229

5.945 ± 0.227

2.833 ± 0.144

7.743 ± 0.25

2.12 ± 0.161

4.513 ± 0.177

3.246 ± 0.211

10.152 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.9 ± 0.121

2.27 ± 0.196

5.489 ± 0.172

3.976 ± 0.23

8.151 ± 0.308

5.259 ± 0.268

5.806 ± 0.275

7.212 ± 0.214

1.663 ± 0.116

2.366 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski