Amino acid dipepetide frequency for Acidithiobacillus phage AcaML1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.333AlaAla: 17.333 ± 1.506
0.966AlaCys: 0.966 ± 0.264
7.781AlaAsp: 7.781 ± 0.802
8.908AlaGlu: 8.908 ± 0.744
2.737AlaPhe: 2.737 ± 0.382
9.445AlaGly: 9.445 ± 0.711
2.093AlaHis: 2.093 ± 0.351
6.118AlaIle: 6.118 ± 0.58
4.4AlaLys: 4.4 ± 0.508
13.684AlaLeu: 13.684 ± 1.024
3.22AlaMet: 3.22 ± 0.426
3.112AlaAsn: 3.112 ± 0.417
5.098AlaPro: 5.098 ± 0.539
6.815AlaGln: 6.815 ± 0.768
9.767AlaArg: 9.767 ± 0.787
6.761AlaSer: 6.761 ± 0.496
6.386AlaThr: 6.386 ± 0.611
8.854AlaVal: 8.854 ± 0.833
1.825AlaTrp: 1.825 ± 0.346
2.79AlaTyr: 2.79 ± 0.347
0.0AlaXaa: 0.0 ± 0.0
Cys
1.127CysAla: 1.127 ± 0.213
0.0CysCys: 0.0 ± 0.0
0.537CysAsp: 0.537 ± 0.186
0.483CysGlu: 0.483 ± 0.144
0.161CysPhe: 0.161 ± 0.092
0.859CysGly: 0.859 ± 0.279
0.107CysHis: 0.107 ± 0.071
0.215CysIle: 0.215 ± 0.089
0.322CysLys: 0.322 ± 0.142
0.859CysLeu: 0.859 ± 0.23
0.054CysMet: 0.054 ± 0.046
0.268CysAsn: 0.268 ± 0.117
0.429CysPro: 0.429 ± 0.175
0.268CysGln: 0.268 ± 0.13
0.483CysArg: 0.483 ± 0.155
0.59CysSer: 0.59 ± 0.216
0.322CysThr: 0.322 ± 0.137
0.322CysVal: 0.322 ± 0.132
0.215CysTrp: 0.215 ± 0.096
0.161CysTyr: 0.161 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
8.747AspAla: 8.747 ± 0.747
0.59AspCys: 0.59 ± 0.141
2.951AspAsp: 2.951 ± 0.33
4.186AspGlu: 4.186 ± 0.452
1.556AspPhe: 1.556 ± 0.317
5.259AspGly: 5.259 ± 0.608
0.966AspHis: 0.966 ± 0.257
2.468AspIle: 2.468 ± 0.316
1.342AspLys: 1.342 ± 0.274
4.991AspLeu: 4.991 ± 0.597
0.859AspMet: 0.859 ± 0.234
1.02AspAsn: 1.02 ± 0.243
4.454AspPro: 4.454 ± 0.528
2.039AspGln: 2.039 ± 0.295
4.239AspArg: 4.239 ± 0.511
1.878AspSer: 1.878 ± 0.335
2.522AspThr: 2.522 ± 0.347
4.561AspVal: 4.561 ± 0.487
1.234AspTrp: 1.234 ± 0.213
1.825AspTyr: 1.825 ± 0.324
0.0AspXaa: 0.0 ± 0.0
Glu
8.21GluAla: 8.21 ± 0.778
0.376GluCys: 0.376 ± 0.134
2.039GluAsp: 2.039 ± 0.25
3.166GluGlu: 3.166 ± 0.426
2.093GluPhe: 2.093 ± 0.314
4.347GluGly: 4.347 ± 0.538
1.503GluHis: 1.503 ± 0.263
3.059GluIle: 3.059 ± 0.4
1.61GluLys: 1.61 ± 0.248
7.083GluLeu: 7.083 ± 0.674
1.342GluMet: 1.342 ± 0.279
0.644GluAsn: 0.644 ± 0.156
3.381GluPro: 3.381 ± 0.396
2.737GluGln: 2.737 ± 0.351
6.279GluArg: 6.279 ± 0.784
2.2GluSer: 2.2 ± 0.281
3.059GluThr: 3.059 ± 0.345
4.561GluVal: 4.561 ± 0.475
0.751GluTrp: 0.751 ± 0.189
1.02GluTyr: 1.02 ± 0.196
0.0GluXaa: 0.0 ± 0.0
Phe
3.434PheAla: 3.434 ± 0.572
0.429PheCys: 0.429 ± 0.154
2.522PheAsp: 2.522 ± 0.309
3.005PheGlu: 3.005 ± 0.407
0.751PhePhe: 0.751 ± 0.168
2.522PheGly: 2.522 ± 0.51
0.698PheHis: 0.698 ± 0.198
0.805PheIle: 0.805 ± 0.243
0.912PheLys: 0.912 ± 0.167
1.878PheLeu: 1.878 ± 0.335
0.483PheMet: 0.483 ± 0.179
1.181PheAsn: 1.181 ± 0.231
1.127PhePro: 1.127 ± 0.213
0.59PheGln: 0.59 ± 0.24
1.503PheArg: 1.503 ± 0.244
1.395PheSer: 1.395 ± 0.243
1.771PheThr: 1.771 ± 0.411
2.2PheVal: 2.2 ± 0.366
0.376PheTrp: 0.376 ± 0.12
0.751PheTyr: 0.751 ± 0.21
0.0PheXaa: 0.0 ± 0.0
Gly
7.781GlyAla: 7.781 ± 0.85
0.59GlyCys: 0.59 ± 0.198
4.4GlyAsp: 4.4 ± 0.639
4.722GlyGlu: 4.722 ± 0.552
3.22GlyPhe: 3.22 ± 0.353
5.474GlyGly: 5.474 ± 0.451
1.878GlyHis: 1.878 ± 0.307
4.132GlyIle: 4.132 ± 0.5
2.898GlyLys: 2.898 ± 0.476
8.103GlyLeu: 8.103 ± 0.774
1.073GlyMet: 1.073 ± 0.247
1.717GlyAsn: 1.717 ± 0.297
2.468GlyPro: 2.468 ± 0.42
3.649GlyGln: 3.649 ± 0.351
6.118GlyArg: 6.118 ± 0.593
5.205GlySer: 5.205 ± 0.899
4.186GlyThr: 4.186 ± 0.474
5.796GlyVal: 5.796 ± 0.583
1.986GlyTrp: 1.986 ± 0.309
2.039GlyTyr: 2.039 ± 0.349
0.0GlyXaa: 0.0 ± 0.0
His
2.898HisAla: 2.898 ± 0.409
0.376HisCys: 0.376 ± 0.15
1.181HisAsp: 1.181 ± 0.236
0.912HisGlu: 0.912 ± 0.23
0.751HisPhe: 0.751 ± 0.172
1.503HisGly: 1.503 ± 0.32
0.805HisHis: 0.805 ± 0.262
0.429HisIle: 0.429 ± 0.17
0.698HisLys: 0.698 ± 0.15
2.146HisLeu: 2.146 ± 0.354
0.215HisMet: 0.215 ± 0.146
0.268HisAsn: 0.268 ± 0.105
1.717HisPro: 1.717 ± 0.329
0.483HisGln: 0.483 ± 0.184
2.093HisArg: 2.093 ± 0.392
1.181HisSer: 1.181 ± 0.264
0.859HisThr: 0.859 ± 0.213
1.288HisVal: 1.288 ± 0.314
0.537HisTrp: 0.537 ± 0.213
0.859HisTyr: 0.859 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
6.279IleAla: 6.279 ± 0.66
0.161IleCys: 0.161 ± 0.084
4.078IleAsp: 4.078 ± 0.547
4.078IleGlu: 4.078 ± 0.576
0.698IlePhe: 0.698 ± 0.168
3.649IleGly: 3.649 ± 0.489
1.02IleHis: 1.02 ± 0.196
0.644IleIle: 0.644 ± 0.239
1.771IleLys: 1.771 ± 0.297
3.166IleLeu: 3.166 ± 0.368
0.268IleMet: 0.268 ± 0.117
1.127IleAsn: 1.127 ± 0.271
2.254IlePro: 2.254 ± 0.312
1.395IleGln: 1.395 ± 0.255
3.166IleArg: 3.166 ± 0.397
2.629IleSer: 2.629 ± 0.425
3.381IleThr: 3.381 ± 0.367
3.649IleVal: 3.649 ± 0.419
0.537IleTrp: 0.537 ± 0.185
1.181IleTyr: 1.181 ± 0.259
0.0IleXaa: 0.0 ± 0.0
Lys
3.864LysAla: 3.864 ± 0.428
0.376LysCys: 0.376 ± 0.157
1.664LysAsp: 1.664 ± 0.312
1.288LysGlu: 1.288 ± 0.248
0.429LysPhe: 0.429 ± 0.148
2.146LysGly: 2.146 ± 0.393
1.127LysHis: 1.127 ± 0.263
1.342LysIle: 1.342 ± 0.276
1.288LysLys: 1.288 ± 0.268
3.864LysLeu: 3.864 ± 0.461
0.483LysMet: 0.483 ± 0.145
0.912LysAsn: 0.912 ± 0.218
2.361LysPro: 2.361 ± 0.366
1.288LysGln: 1.288 ± 0.298
2.361LysArg: 2.361 ± 0.428
1.717LysSer: 1.717 ± 0.271
2.254LysThr: 2.254 ± 0.402
2.468LysVal: 2.468 ± 0.417
0.483LysTrp: 0.483 ± 0.196
0.751LysTyr: 0.751 ± 0.183
0.0LysXaa: 0.0 ± 0.0
Leu
14.06LeuAla: 14.06 ± 1.239
0.644LeuCys: 0.644 ± 0.202
5.688LeuAsp: 5.688 ± 0.476
5.474LeuGlu: 5.474 ± 0.473
2.361LeuPhe: 2.361 ± 0.343
7.942LeuGly: 7.942 ± 0.801
1.771LeuHis: 1.771 ± 0.356
4.722LeuIle: 4.722 ± 0.572
4.239LeuLys: 4.239 ± 0.628
9.606LeuLeu: 9.606 ± 0.817
2.415LeuMet: 2.415 ± 0.255
2.146LeuAsn: 2.146 ± 0.328
6.171LeuPro: 6.171 ± 0.607
3.864LeuGln: 3.864 ± 0.574
8.371LeuArg: 8.371 ± 0.6
6.225LeuSer: 6.225 ± 0.756
6.279LeuThr: 6.279 ± 0.653
5.796LeuVal: 5.796 ± 0.61
1.342LeuTrp: 1.342 ± 0.257
2.415LeuTyr: 2.415 ± 0.288
0.0LeuXaa: 0.0 ± 0.0
Met
2.79MetAla: 2.79 ± 0.44
0.054MetCys: 0.054 ± 0.046
0.805MetAsp: 0.805 ± 0.185
0.859MetGlu: 0.859 ± 0.186
0.161MetPhe: 0.161 ± 0.112
1.449MetGly: 1.449 ± 0.338
0.429MetHis: 0.429 ± 0.151
0.859MetIle: 0.859 ± 0.188
0.912MetLys: 0.912 ± 0.219
1.449MetLeu: 1.449 ± 0.275
0.429MetMet: 0.429 ± 0.143
0.429MetAsn: 0.429 ± 0.151
0.912MetPro: 0.912 ± 0.261
0.805MetGln: 0.805 ± 0.17
1.61MetArg: 1.61 ± 0.312
1.664MetSer: 1.664 ± 0.342
1.878MetThr: 1.878 ± 0.334
1.127MetVal: 1.127 ± 0.238
0.161MetTrp: 0.161 ± 0.081
0.215MetTyr: 0.215 ± 0.093
0.0MetXaa: 0.0 ± 0.0
Asn
3.542AsnAla: 3.542 ± 0.43
0.107AsnCys: 0.107 ± 0.07
1.234AsnAsp: 1.234 ± 0.263
1.825AsnGlu: 1.825 ± 0.255
0.537AsnPhe: 0.537 ± 0.186
1.771AsnGly: 1.771 ± 0.409
0.483AsnHis: 0.483 ± 0.123
0.912AsnIle: 0.912 ± 0.219
0.698AsnLys: 0.698 ± 0.181
2.522AsnLeu: 2.522 ± 0.41
0.161AsnMet: 0.161 ± 0.083
0.215AsnAsn: 0.215 ± 0.165
1.556AsnPro: 1.556 ± 0.373
0.698AsnGln: 0.698 ± 0.203
1.825AsnArg: 1.825 ± 0.271
1.02AsnSer: 1.02 ± 0.273
1.503AsnThr: 1.503 ± 0.277
1.342AsnVal: 1.342 ± 0.244
0.322AsnTrp: 0.322 ± 0.122
0.429AsnTyr: 0.429 ± 0.142
0.0AsnXaa: 0.0 ± 0.0
Pro
6.922ProAla: 6.922 ± 0.577
0.322ProCys: 0.322 ± 0.128
3.971ProAsp: 3.971 ± 0.454
2.844ProGlu: 2.844 ± 0.366
1.288ProPhe: 1.288 ± 0.267
5.044ProGly: 5.044 ± 0.438
1.234ProHis: 1.234 ± 0.276
2.844ProIle: 2.844 ± 0.394
1.556ProLys: 1.556 ± 0.334
4.4ProLeu: 4.4 ± 0.628
1.288ProMet: 1.288 ± 0.297
1.61ProAsn: 1.61 ± 0.263
3.488ProPro: 3.488 ± 0.517
1.986ProGln: 1.986 ± 0.379
3.166ProArg: 3.166 ± 0.388
2.576ProSer: 2.576 ± 0.418
3.22ProThr: 3.22 ± 0.446
4.561ProVal: 4.561 ± 0.495
1.127ProTrp: 1.127 ± 0.246
1.449ProTyr: 1.449 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
5.259GlnAla: 5.259 ± 0.952
0.161GlnCys: 0.161 ± 0.082
1.825GlnAsp: 1.825 ± 0.232
1.932GlnGlu: 1.932 ± 0.337
1.234GlnPhe: 1.234 ± 0.216
3.059GlnGly: 3.059 ± 0.427
1.02GlnHis: 1.02 ± 0.234
2.307GlnIle: 2.307 ± 0.352
1.073GlnLys: 1.073 ± 0.241
4.186GlnLeu: 4.186 ± 0.519
0.698GlnMet: 0.698 ± 0.208
0.483GlnAsn: 0.483 ± 0.197
2.307GlnPro: 2.307 ± 0.33
1.878GlnGln: 1.878 ± 0.337
4.347GlnArg: 4.347 ± 0.45
2.093GlnSer: 2.093 ± 0.407
2.093GlnThr: 2.093 ± 0.352
2.415GlnVal: 2.415 ± 0.389
0.59GlnTrp: 0.59 ± 0.149
0.805GlnTyr: 0.805 ± 0.223
0.0GlnXaa: 0.0 ± 0.0
Arg
8.962ArgAla: 8.962 ± 0.774
0.751ArgCys: 0.751 ± 0.21
3.703ArgAsp: 3.703 ± 0.47
4.454ArgGlu: 4.454 ± 0.565
2.844ArgPhe: 2.844 ± 0.359
4.561ArgGly: 4.561 ± 0.463
1.717ArgHis: 1.717 ± 0.329
4.991ArgIle: 4.991 ± 0.656
2.468ArgLys: 2.468 ± 0.367
11.537ArgLeu: 11.537 ± 0.995
1.825ArgMet: 1.825 ± 0.358
2.093ArgAsn: 2.093 ± 0.287
3.917ArgPro: 3.917 ± 0.407
2.468ArgGln: 2.468 ± 0.372
7.727ArgArg: 7.727 ± 0.783
4.347ArgSer: 4.347 ± 0.518
3.327ArgThr: 3.327 ± 0.363
5.635ArgVal: 5.635 ± 0.593
1.878ArgTrp: 1.878 ± 0.282
2.307ArgTyr: 2.307 ± 0.352
0.0ArgXaa: 0.0 ± 0.0
Ser
6.279SerAla: 6.279 ± 0.933
0.376SerCys: 0.376 ± 0.134
3.059SerAsp: 3.059 ± 0.468
2.468SerGlu: 2.468 ± 0.376
1.61SerPhe: 1.61 ± 0.298
4.776SerGly: 4.776 ± 0.581
1.073SerHis: 1.073 ± 0.263
2.737SerIle: 2.737 ± 0.394
1.556SerLys: 1.556 ± 0.265
4.025SerLeu: 4.025 ± 0.64
1.342SerMet: 1.342 ± 0.316
1.342SerAsn: 1.342 ± 0.383
2.844SerPro: 2.844 ± 0.371
1.664SerGln: 1.664 ± 0.337
4.561SerArg: 4.561 ± 0.46
2.951SerSer: 2.951 ± 0.522
3.864SerThr: 3.864 ± 0.513
4.186SerVal: 4.186 ± 0.46
0.805SerTrp: 0.805 ± 0.203
1.073SerTyr: 1.073 ± 0.323
0.0SerXaa: 0.0 ± 0.0
Thr
6.493ThrAla: 6.493 ± 0.537
0.268ThrCys: 0.268 ± 0.12
3.059ThrAsp: 3.059 ± 0.551
2.951ThrGlu: 2.951 ± 0.458
2.2ThrPhe: 2.2 ± 0.353
5.044ThrGly: 5.044 ± 0.589
0.966ThrHis: 0.966 ± 0.219
2.146ThrIle: 2.146 ± 0.348
1.771ThrLys: 1.771 ± 0.372
6.547ThrLeu: 6.547 ± 0.599
0.859ThrMet: 0.859 ± 0.19
0.966ThrAsn: 0.966 ± 0.243
4.454ThrPro: 4.454 ± 0.508
2.576ThrGln: 2.576 ± 0.321
4.078ThrArg: 4.078 ± 0.476
2.254ThrSer: 2.254 ± 0.444
4.186ThrThr: 4.186 ± 0.555
4.991ThrVal: 4.991 ± 0.627
0.912ThrTrp: 0.912 ± 0.236
1.342ThrTyr: 1.342 ± 0.268
0.0ThrXaa: 0.0 ± 0.0
Val
9.176ValAla: 9.176 ± 0.756
0.698ValCys: 0.698 ± 0.208
3.971ValAsp: 3.971 ± 0.467
4.669ValGlu: 4.669 ± 0.497
2.468ValPhe: 2.468 ± 0.381
5.044ValGly: 5.044 ± 0.614
1.556ValHis: 1.556 ± 0.363
3.273ValIle: 3.273 ± 0.355
1.932ValLys: 1.932 ± 0.316
7.352ValLeu: 7.352 ± 0.568
1.342ValMet: 1.342 ± 0.289
2.361ValAsn: 2.361 ± 0.391
3.703ValPro: 3.703 ± 0.309
2.146ValGln: 2.146 ± 0.411
5.957ValArg: 5.957 ± 0.543
3.488ValSer: 3.488 ± 0.478
4.776ValThr: 4.776 ± 0.803
5.849ValVal: 5.849 ± 0.497
1.02ValTrp: 1.02 ± 0.239
1.288ValTyr: 1.288 ± 0.251
0.0ValXaa: 0.0 ± 0.0
Trp
1.825TrpAla: 1.825 ± 0.331
0.268TrpCys: 0.268 ± 0.12
1.395TrpAsp: 1.395 ± 0.379
0.483TrpGlu: 0.483 ± 0.154
0.429TrpPhe: 0.429 ± 0.172
1.073TrpGly: 1.073 ± 0.223
0.376TrpHis: 0.376 ± 0.153
0.698TrpIle: 0.698 ± 0.16
0.483TrpLys: 0.483 ± 0.158
1.771TrpLeu: 1.771 ± 0.327
0.483TrpMet: 0.483 ± 0.138
0.429TrpAsn: 0.429 ± 0.182
0.859TrpPro: 0.859 ± 0.26
0.912TrpGln: 0.912 ± 0.24
1.771TrpArg: 1.771 ± 0.387
1.073TrpSer: 1.073 ± 0.212
1.02TrpThr: 1.02 ± 0.196
1.073TrpVal: 1.073 ± 0.22
0.107TrpTrp: 0.107 ± 0.071
0.107TrpTyr: 0.107 ± 0.07
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.112TyrAla: 3.112 ± 0.36
0.268TyrCys: 0.268 ± 0.116
1.825TyrAsp: 1.825 ± 0.302
0.751TyrGlu: 0.751 ± 0.189
0.805TyrPhe: 0.805 ± 0.193
2.468TyrGly: 2.468 ± 0.459
0.376TyrHis: 0.376 ± 0.159
0.429TyrIle: 0.429 ± 0.154
0.537TyrLys: 0.537 ± 0.198
2.361TyrLeu: 2.361 ± 0.362
0.107TyrMet: 0.107 ± 0.074
0.376TyrAsn: 0.376 ± 0.132
1.395TyrPro: 1.395 ± 0.351
1.503TyrGln: 1.503 ± 0.321
2.093TyrArg: 2.093 ± 0.334
1.342TyrSer: 1.342 ± 0.304
1.181TyrThr: 1.181 ± 0.27
1.449TyrVal: 1.449 ± 0.272
0.429TyrTrp: 0.429 ± 0.137
0.859TyrTyr: 0.859 ± 0.28
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (18636 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski