Desulfomicrobium baculatum (strain DSM 4028 / VKM B-1378 / X) (Desulfovibrio baculatus)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfomicrobiaceae; Desulfomicrobium; Desulfomicrobium baculatum

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3421 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7LVB6|C7LVB6_DESBD Uncharacterized protein OS=Desulfomicrobium baculatum (strain DSM 4028 / VKM B-1378 / X) OX=525897 GN=Dbac_0331 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 10.55RR3 pKa = 11.84FTLVALVAALLFSMVTSASATEE25 pKa = 4.01LKK27 pKa = 10.8VRR29 pKa = 11.84GNFDD33 pKa = 3.51VYY35 pKa = 11.44GMWSSNLNDD44 pKa = 4.22HH45 pKa = 7.31DD46 pKa = 5.53SDD48 pKa = 4.33VADD51 pKa = 3.87GDD53 pKa = 3.91NYY55 pKa = 8.89TTVQRR60 pKa = 11.84MRR62 pKa = 11.84TYY64 pKa = 10.51FDD66 pKa = 3.36YY67 pKa = 11.23VANEE71 pKa = 3.76NLKK74 pKa = 10.69AVLGFEE80 pKa = 4.85FDD82 pKa = 4.17NVWGAGEE89 pKa = 3.95EE90 pKa = 4.51ANWGTDD96 pKa = 2.95GKK98 pKa = 9.41EE99 pKa = 3.98TLEE102 pKa = 4.21VKK104 pKa = 10.08HH105 pKa = 6.72AYY107 pKa = 10.52LDD109 pKa = 4.49FIFPDD114 pKa = 3.66TQIEE118 pKa = 4.4VQAGLQYY125 pKa = 10.6IALPSIFGNPVFDD138 pKa = 5.84DD139 pKa = 3.88DD140 pKa = 4.2AAAITVAAPINDD152 pKa = 3.61MFGVTVGYY160 pKa = 8.61TRR162 pKa = 11.84GTDD165 pKa = 3.4MSNSFSDD172 pKa = 3.66EE173 pKa = 4.08LLVSEE178 pKa = 6.2GYY180 pKa = 10.79DD181 pKa = 3.61KK182 pKa = 11.61DD183 pKa = 4.08DD184 pKa = 3.42VDD186 pKa = 3.96MAFIATPVTLDD197 pKa = 3.56GVSVTPYY204 pKa = 10.25FGYY207 pKa = 11.0AWLGQNSTKK216 pKa = 10.8SFDD219 pKa = 4.1ANDD222 pKa = 4.25DD223 pKa = 3.61STVWVVGANAALTMFDD239 pKa = 4.55PLTFAADD246 pKa = 4.66LIYY249 pKa = 11.24GEE251 pKa = 4.97GDD253 pKa = 2.87SDD255 pKa = 4.34AYY257 pKa = 8.46EE258 pKa = 4.2TKK260 pKa = 10.24GWYY263 pKa = 9.24AALAASYY270 pKa = 10.97KK271 pKa = 9.73MDD273 pKa = 3.85MLTATLFTTYY283 pKa = 9.87ATGYY287 pKa = 10.98DD288 pKa = 4.05EE289 pKa = 6.3DD290 pKa = 4.23TDD292 pKa = 4.46EE293 pKa = 5.6DD294 pKa = 4.38DD295 pKa = 5.31KK296 pKa = 11.91LPTLAEE302 pKa = 4.33GWGLTPYY309 pKa = 10.65VGGDD313 pKa = 3.08RR314 pKa = 11.84AFIAANDD321 pKa = 3.97SFGTDD326 pKa = 3.02ALGVGNDD333 pKa = 3.92GTGLWVLGLVLDD345 pKa = 5.88DD346 pKa = 4.27ISFVDD351 pKa = 3.78KK352 pKa = 10.92LSHH355 pKa = 6.52KK356 pKa = 10.58LVIAYY361 pKa = 8.82AQGTSDD367 pKa = 4.15EE368 pKa = 4.91DD369 pKa = 3.38ATKK372 pKa = 10.5PSGAAVKK379 pKa = 8.83FTEE382 pKa = 5.49EE383 pKa = 3.96DD384 pKa = 3.9DD385 pKa = 4.86LWEE388 pKa = 4.01LWLVNKK394 pKa = 9.99YY395 pKa = 9.63MIYY398 pKa = 10.86EE399 pKa = 3.87NLAAINEE406 pKa = 4.24LGYY409 pKa = 10.15FQASSEE415 pKa = 4.4VYY417 pKa = 10.46EE418 pKa = 4.91DD419 pKa = 3.49ATGDD423 pKa = 4.26DD424 pKa = 4.29LDD426 pKa = 3.73SSYY429 pKa = 11.03FATVGFQYY437 pKa = 11.1KK438 pKa = 9.31FF439 pKa = 3.23

Molecular weight: 47.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7LXK2|C7LXK2_DESBD Uncharacterized protein OS=Desulfomicrobium baculatum (strain DSM 4028 / VKM B-1378 / X) OX=525897 GN=Dbac_3163 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.94RR4 pKa = 11.84TYY6 pKa = 10.32QPSTTKK12 pKa = 10.4RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.09GFLVRR22 pKa = 11.84SRR24 pKa = 11.84TKK26 pKa = 10.46NGQAVLRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.65GRR40 pKa = 11.84KK41 pKa = 8.66RR42 pKa = 11.84LAVV45 pKa = 3.41

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3421

0

3421

1171290

30

8980

342.4

37.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.738 ± 0.051

1.322 ± 0.023

5.524 ± 0.063

6.277 ± 0.042

4.126 ± 0.034

8.106 ± 0.079

2.181 ± 0.021

5.548 ± 0.039

4.259 ± 0.044

10.924 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.764 ± 0.025

3.024 ± 0.033

4.632 ± 0.041

3.416 ± 0.023

6.237 ± 0.053

5.856 ± 0.05

5.106 ± 0.046

7.258 ± 0.041

1.196 ± 0.018

2.505 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski