Simian foamy virus type 1 (SFVmac) (SFV-1)
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P29170|BEL2_SFV1 Protein Bel-2 OS=Simian foamy virus type 1 OX=338478 GN=bel2 PE=3 SV=2
MM1 pKa = 7.56 ASWEE5 pKa = 4.1 AQEE8 pKa = 4.1 EE9 pKa = 4.25 LRR11 pKa = 11.84 EE12 pKa = 4.17 LLHH15 pKa = 6.6 HH16 pKa = 6.8 LPEE19 pKa = 5.62 DD20 pKa = 4.55 DD21 pKa = 4.46 PPADD25 pKa = 3.64 LTHH28 pKa = 7.16 LLEE31 pKa = 5.78 LDD33 pKa = 3.35 EE34 pKa = 4.89 MEE36 pKa = 4.75 PKK38 pKa = 10.2 VLCGEE43 pKa = 4.18 NPGDD47 pKa = 3.85 EE48 pKa = 4.31 KK49 pKa = 11.2 LKK51 pKa = 10.45 KK52 pKa = 10.03 QVIKK56 pKa = 9.27 TPPMHH61 pKa = 7.15 PSTVTWHH68 pKa = 6.47 FGYY71 pKa = 9.84 KK72 pKa = 9.48 QKK74 pKa = 10.86 EE75 pKa = 4.1 DD76 pKa = 3.4 QQDD79 pKa = 3.83 NIKK82 pKa = 9.66 MRR84 pKa = 11.84 DD85 pKa = 3.41 WVPDD89 pKa = 3.82 PSKK92 pKa = 10.42 MSKK95 pKa = 8.36 STCKK99 pKa = 10.39 RR100 pKa = 11.84 LILLGLYY107 pKa = 7.27 QACKK111 pKa = 9.01 AQEE114 pKa = 4.39 IIKK117 pKa = 9.57 MDD119 pKa = 3.96 YY120 pKa = 10.13 DD121 pKa = 3.89 VHH123 pKa = 6.13 WEE125 pKa = 3.82 KK126 pKa = 11.18 SVVNEE131 pKa = 3.75 QYY133 pKa = 11.1 FEE135 pKa = 4.19 VEE137 pKa = 4.7 YY138 pKa = 10.31 NCKK141 pKa = 8.54 MCRR144 pKa = 11.84 TVLHH148 pKa = 6.47 EE149 pKa = 4.96 PMPIMYY155 pKa = 10.24 DD156 pKa = 3.43 PEE158 pKa = 4.08 TEE160 pKa = 4.08 LWVKK164 pKa = 10.27 PGRR167 pKa = 11.84 LRR169 pKa = 11.84 GPLGSAVYY177 pKa = 8.72 TLKK180 pKa = 10.62 KK181 pKa = 10.05 HH182 pKa = 5.97 YY183 pKa = 8.69 EE184 pKa = 4.03 RR185 pKa = 11.84 CLLTLPSLKK194 pKa = 9.45 GTRR197 pKa = 11.84 LPKK200 pKa = 10.07 RR201 pKa = 11.84 RR202 pKa = 11.84 CNPSRR207 pKa = 11.84 RR208 pKa = 11.84 YY209 pKa = 7.26 EE210 pKa = 4.14 TFRR213 pKa = 11.84 EE214 pKa = 4.21 HH215 pKa = 6.78 PPTRR219 pKa = 11.84 KK220 pKa = 9.35 RR221 pKa = 11.84 RR222 pKa = 11.84 SKK224 pKa = 10.85 EE225 pKa = 3.95 GIPTDD230 pKa = 3.8 QQPSTSNGDD239 pKa = 3.43 PMALLSGPCGPHH251 pKa = 6.76 SIQPPSCLLQEE262 pKa = 4.21 LPKK265 pKa = 10.48 PEE267 pKa = 4.08 VGSPEE272 pKa = 3.94 MAVAMSGGPFWEE284 pKa = 4.27 EE285 pKa = 4.24 VYY287 pKa = 10.97 GDD289 pKa = 4.52 SIFATPLGSSEE300 pKa = 4.58 DD301 pKa = 3.62 QLLSQFDD308 pKa = 3.43
Molecular weight: 35.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.597
IPC2_protein 5.601
IPC_protein 5.614
Toseland 5.919
ProMoST 5.804
Dawson 5.83
Bjellqvist 5.855
Wikipedia 5.804
Rodwell 5.804
Grimsley 6.033
Solomon 5.83
Lehninger 5.817
Nozaki 6.071
DTASelect 6.249
Thurlkill 6.224
EMBOSS 6.186
Sillero 6.173
Patrickios 4.113
IPC_peptide 5.842
IPC2_peptide 6.16
IPC2.peptide.svr19 6.168
Protein with the highest isoelectric point:
>sp|Q00071|GAG_SFV1 Gag polyprotein OS=Simian foamy virus type 1 OX=338478 GN=gag PE=3 SV=1
MM1 pKa = 7.41 AAIEE5 pKa = 4.57 GDD7 pKa = 4.0 LDD9 pKa = 3.84 VQALANLFNDD19 pKa = 4.45 LGINRR24 pKa = 11.84 NPRR27 pKa = 11.84 HH28 pKa = 6.19 RR29 pKa = 11.84 EE30 pKa = 3.83 VIALRR35 pKa = 11.84 MTGGWWGPATRR46 pKa = 11.84 YY47 pKa = 10.44 NLVSLLLQDD56 pKa = 5.34 DD57 pKa = 3.84 QGQPLPQPRR66 pKa = 11.84 WRR68 pKa = 11.84 AEE70 pKa = 3.45 GRR72 pKa = 11.84 AANPAVMFTLEE83 pKa = 5.27 APWQDD88 pKa = 3.54 LRR90 pKa = 11.84 LAFDD94 pKa = 4.65 NIDD97 pKa = 3.54 VGEE100 pKa = 4.31 GTLRR104 pKa = 11.84 FGPLANGNYY113 pKa = 9.59 IPGDD117 pKa = 3.63 EE118 pKa = 4.68 FSLEE122 pKa = 4.0 FLPPAMQEE130 pKa = 3.67 ITQMQRR136 pKa = 11.84 DD137 pKa = 4.04 EE138 pKa = 4.64 LEE140 pKa = 3.98 EE141 pKa = 3.91 VLDD144 pKa = 3.93 VVGQITMQMNDD155 pKa = 4.33 LIGMQDD161 pKa = 3.02 AQIRR165 pKa = 11.84 GLEE168 pKa = 4.03 GQLRR172 pKa = 11.84 GLRR175 pKa = 11.84 GNLPVAGTPPPPPPSLDD192 pKa = 3.66 LQPAAASSPYY202 pKa = 9.75 VAPAPSAPAASAAAADD218 pKa = 4.58 LGWFAGGPGPGSLDD232 pKa = 3.22 PRR234 pKa = 11.84 LARR237 pKa = 11.84 VAYY240 pKa = 10.38 NPFLPGPSDD249 pKa = 3.67 GSGAAPAQPSAPPVASPLPSLLPAQPMQPVIQYY282 pKa = 7.04 VHH284 pKa = 7.22 PPPINPAQQVIPIQHH299 pKa = 5.77 IRR301 pKa = 11.84 AVTGNAPSNPRR312 pKa = 11.84 EE313 pKa = 3.63 IPMWIGRR320 pKa = 11.84 NASAIEE326 pKa = 4.27 GVFPIPTSDD335 pKa = 2.67 IRR337 pKa = 11.84 SRR339 pKa = 11.84 VINALLGRR347 pKa = 11.84 QLGLNLDD354 pKa = 4.01 PQHH357 pKa = 6.93 CITWASAIATLYY369 pKa = 10.37 VRR371 pKa = 11.84 THH373 pKa = 5.88 GSYY376 pKa = 10.42 PLHH379 pKa = 6.18 QLAEE383 pKa = 4.11 VLRR386 pKa = 11.84 RR387 pKa = 11.84 VSNSEE392 pKa = 3.39 GAAAAWQLGMMLTNQDD408 pKa = 3.87 YY409 pKa = 9.96 NLVWGMVRR417 pKa = 11.84 PLLPGQAVVTAMQHH431 pKa = 6.74 RR432 pKa = 11.84 LDD434 pKa = 4.41 QEE436 pKa = 4.08 VSDD439 pKa = 4.06 AARR442 pKa = 11.84 IVSFVNHH449 pKa = 6.37 LNAVYY454 pKa = 10.41 EE455 pKa = 4.03 LLGLNARR462 pKa = 11.84 GQNLRR467 pKa = 11.84 VSTGGQTTARR477 pKa = 11.84 TSAGRR482 pKa = 11.84 GARR485 pKa = 11.84 GRR487 pKa = 11.84 RR488 pKa = 11.84 SQQGTPGRR496 pKa = 11.84 QSSGQAPPQGRR507 pKa = 11.84 RR508 pKa = 11.84 SSQGQQPRR516 pKa = 11.84 QSEE519 pKa = 4.19 SGDD522 pKa = 3.46 QNNQRR527 pKa = 11.84 QLQGGNNRR535 pKa = 11.84 GGYY538 pKa = 7.32 NLRR541 pKa = 11.84 PRR543 pKa = 11.84 TYY545 pKa = 9.41 QPQRR549 pKa = 11.84 YY550 pKa = 8.66 GGGRR554 pKa = 11.84 GRR556 pKa = 11.84 RR557 pKa = 11.84 WNDD560 pKa = 2.72 QTARR564 pKa = 11.84 ADD566 pKa = 3.53 NQQRR570 pKa = 11.84 SQSQQPQSEE579 pKa = 4.15 ARR581 pKa = 11.84 GEE583 pKa = 3.9 QSRR586 pKa = 11.84 TSGAGRR592 pKa = 11.84 EE593 pKa = 3.92 QGGRR597 pKa = 11.84 GNQNRR602 pKa = 11.84 NQRR605 pKa = 11.84 SAGEE609 pKa = 4.01 NTDD612 pKa = 3.32 RR613 pKa = 11.84 SVNTVTATSASISASGQNGSSTTPPASGSGNQGNN647 pKa = 3.64
Molecular weight: 69.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.818
IPC2_protein 9.209
IPC_protein 10.394
Toseland 9.458
ProMoST 9.692
Dawson 9.897
Bjellqvist 9.823
Wikipedia 10.262
Rodwell 9.78
Grimsley 10.072
Solomon 9.97
Lehninger 9.897
Nozaki 9.502
DTASelect 9.809
Thurlkill 9.663
EMBOSS 9.94
Sillero 9.823
Patrickios 5.067
IPC_peptide 9.955
IPC2_peptide 9.092
IPC2.peptide.svr19 8.129
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3496
308
1149
699.2
78.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.522 ± 0.945
1.602 ± 0.59
4.577 ± 0.192
5.578 ± 0.554
2.603 ± 0.282
5.721 ± 1.232
2.574 ± 0.293
5.606 ± 0.849
5.578 ± 1.309
9.983 ± 0.409
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.174 ± 0.262
4.863 ± 0.422
7.437 ± 0.919
6.121 ± 0.732
4.977 ± 0.886
6.922 ± 0.143
5.521 ± 0.479
5.778 ± 0.394
2.031 ± 0.152
3.833 ± 0.501
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here