Desulfotignum phosphitoxidans DSM 13687

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfotignum; Desulfotignum phosphitoxidans

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4515 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0G4F0|S0G4F0_9DELT Excisionase DNA binding family protein OS=Desulfotignum phosphitoxidans DSM 13687 OX=1286635 GN=Dpo_5c01100 PE=4 SV=1
MM1 pKa = 7.9PLPGFDD7 pKa = 3.2KK8 pKa = 10.75AYY10 pKa = 10.42YY11 pKa = 9.87LQAKK15 pKa = 7.96LTALQTTLPEE25 pKa = 4.27WVGKK29 pKa = 6.57TTTDD33 pKa = 4.76LEE35 pKa = 4.53TLLTNQNFTAEE46 pKa = 4.08SHH48 pKa = 5.37YY49 pKa = 10.92QLYY52 pKa = 10.55GYY54 pKa = 9.69QEE56 pKa = 4.2GLAPNAFFNADD67 pKa = 3.53EE68 pKa = 4.18YY69 pKa = 9.87TQAKK73 pKa = 8.41AVSMFNAGGYY83 pKa = 7.52STISDD88 pKa = 2.99ARR90 pKa = 11.84AAFVAAWTGDD100 pKa = 3.54VYY102 pKa = 11.72NHH104 pKa = 5.88YY105 pKa = 10.04LQYY108 pKa = 11.17GSQEE112 pKa = 4.38GINPSNAFDD121 pKa = 3.53EE122 pKa = 4.48SSYY125 pKa = 10.69YY126 pKa = 10.57ASKK129 pKa = 10.77LADD132 pKa = 3.66LQANADD138 pKa = 4.05TSAEE142 pKa = 3.99WATKK146 pKa = 10.28SVAEE150 pKa = 4.46LKK152 pKa = 10.99AFFNAQGFSALHH164 pKa = 6.47HH165 pKa = 5.94YY166 pKa = 9.72NVYY169 pKa = 10.4GAGEE173 pKa = 5.04GIPVTEE179 pKa = 4.39VPVSEE184 pKa = 4.45AVNSEE189 pKa = 3.57TDD191 pKa = 3.7EE192 pKa = 4.42IDD194 pKa = 3.4EE195 pKa = 4.32TDD197 pKa = 3.52EE198 pKa = 4.04TDD200 pKa = 3.39EE201 pKa = 4.84TGGTPAKK208 pKa = 10.47GMNFQLTTNDD218 pKa = 3.46DD219 pKa = 3.98SFTGGAANDD228 pKa = 4.12HH229 pKa = 6.87FYY231 pKa = 11.36GIYY234 pKa = 10.69DD235 pKa = 3.69GVTFLAQNTFNSGDD249 pKa = 3.62ILDD252 pKa = 4.59GAGGTDD258 pKa = 3.28TLHH261 pKa = 6.66INHH264 pKa = 6.42FEE266 pKa = 4.11DD267 pKa = 4.34VAIDD271 pKa = 4.32LPDD274 pKa = 3.89TLWTGISGIEE284 pKa = 4.21NIVMSTTGSGAHH296 pKa = 7.16DD297 pKa = 5.35IITGTNFNTAFASGGVDD314 pKa = 3.15LWTATTGAGAITIDD328 pKa = 3.48MASFTGTAKK337 pKa = 9.13VTADD341 pKa = 3.34SAAGAIVLSGVKK353 pKa = 10.31FSQIDD358 pKa = 3.56VTTTGSGAQTITSTGASDD376 pKa = 4.08VIVNATSSEE385 pKa = 4.1GATSITTGAGDD396 pKa = 3.69DD397 pKa = 4.38TIALHH402 pKa = 5.87ATSADD407 pKa = 3.28GLNTITAGAGADD419 pKa = 4.03TINLYY424 pKa = 11.39ANFASIDD431 pKa = 3.92KK432 pKa = 10.29IVQADD437 pKa = 4.1GDD439 pKa = 4.64SMASTANTITSNIATGDD456 pKa = 3.65TITFGNGLDD465 pKa = 3.13IVRR468 pKa = 11.84NFGVNDD474 pKa = 4.05NIDD477 pKa = 3.61VGTGGAAVSGLTMDD491 pKa = 4.39KK492 pKa = 11.14ADD494 pKa = 4.09FAATTTIFLSGTYY507 pKa = 10.43AEE509 pKa = 4.61DD510 pKa = 3.66TFTIAADD517 pKa = 3.66GGGTDD522 pKa = 3.99TLLLDD527 pKa = 3.81TTAVDD532 pKa = 3.87DD533 pKa = 4.27TDD535 pKa = 3.85IATADD540 pKa = 3.24TWILLSGVTSDD551 pKa = 4.2ALSAGSFVV559 pKa = 3.47

Molecular weight: 57.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0FWG3|S0FWG3_9DELT 4HBT domain-containing protein OS=Desulfotignum phosphitoxidans DSM 13687 OX=1286635 GN=Dpo_6c02590 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 7.64RR12 pKa = 11.84TRR14 pKa = 11.84KK15 pKa = 9.77HH16 pKa = 5.74GFLKK20 pKa = 10.65RR21 pKa = 11.84MSTPGGKK28 pKa = 8.89RR29 pKa = 11.84VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LALL44 pKa = 3.85

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4515

0

4515

1467878

29

3891

325.1

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.4 ± 0.04

1.367 ± 0.017

5.959 ± 0.031

5.648 ± 0.028

4.57 ± 0.028

7.201 ± 0.036

2.348 ± 0.016

6.937 ± 0.035

5.741 ± 0.033

9.696 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.972 ± 0.018

3.55 ± 0.025

4.624 ± 0.023

3.726 ± 0.024

5.375 ± 0.027

5.471 ± 0.021

5.671 ± 0.025

6.803 ± 0.03

1.112 ± 0.014

2.831 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski