Microbacterium sp. Root61

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3629 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T2L1A0|A0A0T2L1A0_9MICO Peptide synthetase OS=Microbacterium sp. Root61 OX=1736570 GN=ASD65_15575 PE=4 SV=1
MM1 pKa = 7.81AGTPTQNPDD10 pKa = 3.01ASWNISYY17 pKa = 8.93TITVKK22 pKa = 10.93NPGASPISATLTDD35 pKa = 4.9AFPATPAGWTLAGGVWNIAAQGGAPITNTTSAASPIWSGTLPANTTYY82 pKa = 10.6TYY84 pKa = 10.34IVSGKK89 pKa = 8.27LTPTAGATPIGDD101 pKa = 4.03CQTQGKK107 pKa = 9.19GLTNTATVTSGSVSDD122 pKa = 3.78TSGDD126 pKa = 3.74CVSIVTPPVTVTKK139 pKa = 10.46TDD141 pKa = 3.37GTVSQLVDD149 pKa = 3.66GTWQIDD155 pKa = 3.6YY156 pKa = 9.35TVTVTNGGTQATVYY170 pKa = 9.87TLTDD174 pKa = 3.55TPDD177 pKa = 3.24LGTGFTLVSGTWFGPAPVANTPIEE201 pKa = 4.39GGGFDD206 pKa = 3.2QYY208 pKa = 11.01VYY210 pKa = 10.87RR211 pKa = 11.84VIASFNPATPDD222 pKa = 3.88PEE224 pKa = 4.5LTCDD228 pKa = 3.49TTNGGAFFNKK238 pKa = 10.2ALVTFPGGTDD248 pKa = 3.7DD249 pKa = 5.27DD250 pKa = 4.67TGCGEE255 pKa = 4.25PEE257 pKa = 4.08SPTVEE262 pKa = 4.13KK263 pKa = 10.55SASAATQAPGGEE275 pKa = 3.9WTLTYY280 pKa = 10.31TVTVDD285 pKa = 3.44NTSEE289 pKa = 3.79MALAYY294 pKa = 7.62TASDD298 pKa = 3.66TPAALPAGVTLTTPWAVTGPAALNGGTATLTPGWTGTAPNTQFATGHH345 pKa = 6.41LLAGTAHH352 pKa = 7.27TYY354 pKa = 6.94TVKK357 pKa = 10.99AGVTLSAGVTPATLTCGQTPGGNGFWNSATVTNGVGTSDD396 pKa = 5.12DD397 pKa = 4.17SACVTVPFDD406 pKa = 4.12DD407 pKa = 3.76VGIVKK412 pKa = 7.88TTEE415 pKa = 4.19GVDD418 pKa = 3.84GPVEE422 pKa = 3.9SDD424 pKa = 3.59GVFKK428 pKa = 11.18YY429 pKa = 11.04VLTVTNHH436 pKa = 4.5GTRR439 pKa = 11.84AATNVKK445 pKa = 8.63VTDD448 pKa = 4.15PVPSRR453 pKa = 11.84LTVTGIDD460 pKa = 3.56LTDD463 pKa = 3.28ATGWTNDD470 pKa = 3.45NDD472 pKa = 4.05PDD474 pKa = 4.37FVGEE478 pKa = 4.31GNTVDD483 pKa = 3.34LTGPVSFGVGATAEE497 pKa = 3.98IVLTVKK503 pKa = 10.23VNPVPVPEE511 pKa = 4.4IPNLNEE517 pKa = 4.0GDD519 pKa = 3.93PVPTPEE525 pKa = 5.15LPMSTLVNEE534 pKa = 4.6ACVSADD540 pKa = 3.2MDD542 pKa = 4.34SVPGNDD548 pKa = 3.67CDD550 pKa = 4.34SVTVEE555 pKa = 4.54TKK557 pKa = 10.51DD558 pKa = 2.92IAAIVYY564 pKa = 7.03TRR566 pKa = 11.84CVGDD570 pKa = 3.47APLIGFVVAKK580 pKa = 9.1TPNLAALPVDD590 pKa = 4.72FTWTPNSPEE599 pKa = 4.58PDD601 pKa = 3.3TDD603 pKa = 3.6PAEE606 pKa = 4.27VAKK609 pKa = 10.37QYY611 pKa = 10.73PGGTATVSDD620 pKa = 3.6EE621 pKa = 4.24FAWVGTAFTPSGVSLDD637 pKa = 3.94YY638 pKa = 10.9PGWRR642 pKa = 11.84ALQASDD648 pKa = 3.65YY649 pKa = 10.64APGGGYY655 pKa = 9.69YY656 pKa = 9.75IPGTTDD662 pKa = 2.44VMTPTDD668 pKa = 4.06EE669 pKa = 4.83EE670 pKa = 4.09EE671 pKa = 4.22MIFNGLILDD680 pKa = 4.11PSEE683 pKa = 5.77LDD685 pKa = 3.61YY686 pKa = 11.45AWRR689 pKa = 11.84DD690 pKa = 3.44TTTVVLSVNPSMTFTVEE707 pKa = 4.17YY708 pKa = 10.16PDD710 pKa = 3.69ATPEE714 pKa = 4.18CFVARR719 pKa = 11.84HH720 pKa = 5.33TEE722 pKa = 3.92VQIEE726 pKa = 4.17KK727 pKa = 8.77TASVEE732 pKa = 3.9KK733 pKa = 9.81TDD735 pKa = 3.71PGKK738 pKa = 11.04SFTYY742 pKa = 9.86TLAAANVSDD751 pKa = 5.46DD752 pKa = 3.59SAADD756 pKa = 3.69GVVVTDD762 pKa = 5.32TIPADD767 pKa = 3.8LKK769 pKa = 10.17ITDD772 pKa = 3.85VSWTGKK778 pKa = 10.11GDD780 pKa = 4.0ANVFPNWSTCAVSGQNGAGYY800 pKa = 10.49GGTLTCEE807 pKa = 4.27LFGPLQPAGSGLGASAAPTITLSATVNASSKK838 pKa = 11.03ASVITNVGVVDD849 pKa = 4.38YY850 pKa = 8.91YY851 pKa = 11.43TFGDD855 pKa = 3.73PTDD858 pKa = 3.63TGRR861 pKa = 11.84DD862 pKa = 3.2ADD864 pKa = 4.12DD865 pKa = 4.14AVVLLSGLPATGGSALTPLILLGFLALLGGTATIVMIRR903 pKa = 11.84RR904 pKa = 11.84RR905 pKa = 11.84RR906 pKa = 11.84GSTKK910 pKa = 9.98PQLL913 pKa = 3.7

Molecular weight: 93.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T2KYN8|A0A0T2KYN8_9MICO HTH tetR-type domain-containing protein OS=Microbacterium sp. Root61 OX=1736570 GN=ASD65_10680 PE=4 SV=1
MM1 pKa = 7.57LGRR4 pKa = 11.84RR5 pKa = 11.84VGRR8 pKa = 11.84VGVIGAPVAKK18 pKa = 10.0AAVVGAAITPGPAPIAKK35 pKa = 9.67AAVVGAAVTPGRR47 pKa = 11.84SPVAKK52 pKa = 9.48AAVVGAVATPRR63 pKa = 11.84FRR65 pKa = 11.84RR66 pKa = 11.84II67 pKa = 3.12

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3629

0

3629

1208867

38

2358

333.1

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.506 ± 0.062

0.507 ± 0.01

6.225 ± 0.035

5.533 ± 0.041

3.219 ± 0.024

8.944 ± 0.034

1.949 ± 0.022

4.893 ± 0.031

1.85 ± 0.028

10.079 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.919 ± 0.017

1.944 ± 0.024

5.412 ± 0.028

2.745 ± 0.021

6.978 ± 0.051

5.571 ± 0.029

6.185 ± 0.041

9.0 ± 0.042

1.549 ± 0.017

1.992 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski