Amino acid dipepetide frequency for Microbacterium sp. Root61

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.749AlaAla: 20.749 ± 0.225	0.641AlaCys: 0.641 ± 0.027	8.294AlaAsp: 8.294 ± 0.096	7.846AlaGlu: 7.846 ± 0.096	4.043AlaPhe: 4.043 ± 0.063	11.891AlaGly: 11.891 ± 0.11	2.502AlaHis: 2.502 ± 0.048	6.432AlaIle: 6.432 ± 0.081	2.535AlaLys: 2.535 ± 0.058	14.107AlaLeu: 14.107 ± 0.139	2.877AlaMet: 2.877 ± 0.048	2.281AlaAsn: 2.281 ± 0.052	6.614AlaPro: 6.614 ± 0.11	3.913AlaGln: 3.913 ± 0.058	8.954AlaArg: 8.954 ± 0.104	7.568AlaSer: 7.568 ± 0.084	7.776AlaThr: 7.776 ± 0.085	11.648AlaVal: 11.648 ± 0.116	1.971AlaTrp: 1.971 ± 0.039	2.415AlaTyr: 2.415 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.701CysAla: 0.701 ± 0.025	0.044CysCys: 0.044 ± 0.007	0.323CysAsp: 0.323 ± 0.017	0.27CysGlu: 0.27 ± 0.015	0.173CysPhe: 0.173 ± 0.013	0.533CysGly: 0.533 ± 0.02	0.11CysHis: 0.11 ± 0.01	0.229CysIle: 0.229 ± 0.016	0.058CysLys: 0.058 ± 0.007	0.442CysLeu: 0.442 ± 0.019	0.087CysMet: 0.087 ± 0.011	0.114CysAsn: 0.114 ± 0.009	0.255CysPro: 0.255 ± 0.015	0.122CysGln: 0.122 ± 0.01	0.295CysArg: 0.295 ± 0.017	0.319CysSer: 0.319 ± 0.017	0.386CysThr: 0.386 ± 0.023	0.455CysVal: 0.455 ± 0.022	0.065CysTrp: 0.065 ± 0.007	0.093CysTyr: 0.093 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	9.367AspAla: 9.367 ± 0.1	0.271AspCys: 0.271 ± 0.018	4.076AspAsp: 4.076 ± 0.066	4.227AspGlu: 4.227 ± 0.065	1.782AspPhe: 1.782 ± 0.039	6.15AspGly: 6.15 ± 0.083	1.128AspHis: 1.128 ± 0.035	2.635AspIle: 2.635 ± 0.049	1.008AspLys: 1.008 ± 0.036	6.209AspLeu: 6.209 ± 0.069	0.905AspMet: 0.905 ± 0.029	0.93AspAsn: 0.93 ± 0.034	4.309AspPro: 4.309 ± 0.06	1.5AspGln: 1.5 ± 0.04	4.376AspArg: 4.376 ± 0.066	2.579AspSer: 2.579 ± 0.055	3.044AspThr: 3.044 ± 0.052	5.527AspVal: 5.527 ± 0.073	0.927AspTrp: 0.927 ± 0.029	1.296AspTyr: 1.296 ± 0.032	0.001AspXaa: 0.001 ± 0.001
Glu	6.835GluAla: 6.835 ± 0.091	0.242GluCys: 0.242 ± 0.014	2.786GluAsp: 2.786 ± 0.052	2.863GluGlu: 2.863 ± 0.058	1.803GluPhe: 1.803 ± 0.042	4.054GluGly: 4.054 ± 0.066	1.367GluHis: 1.367 ± 0.032	2.808GluIle: 2.808 ± 0.048	1.323GluLys: 1.323 ± 0.04	6.504GluLeu: 6.504 ± 0.082	0.98GluMet: 0.98 ± 0.031	1.252GluAsn: 1.252 ± 0.035	2.928GluPro: 2.928 ± 0.052	1.952GluGln: 1.952 ± 0.041	4.782GluArg: 4.782 ± 0.067	3.056GluSer: 3.056 ± 0.056	3.002GluThr: 3.002 ± 0.054	4.627GluVal: 4.627 ± 0.077	0.96GluTrp: 0.96 ± 0.029	1.208GluTyr: 1.208 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.484PheAla: 4.484 ± 0.061	0.172PheCys: 0.172 ± 0.013	2.406PheAsp: 2.406 ± 0.05	1.748PheGlu: 1.748 ± 0.04	1.102PhePhe: 1.102 ± 0.033	3.541PheGly: 3.541 ± 0.061	0.571PheHis: 0.571 ± 0.024	1.337PheIle: 1.337 ± 0.043	0.407PheLys: 0.407 ± 0.019	2.883PheLeu: 2.883 ± 0.055	0.483PheMet: 0.483 ± 0.017	0.676PheAsn: 0.676 ± 0.024	1.472PhePro: 1.472 ± 0.038	0.834PheGln: 0.834 ± 0.026	1.811PheArg: 1.811 ± 0.039	1.807PheSer: 1.807 ± 0.046	2.268PheThr: 2.268 ± 0.039	3.012PheVal: 3.012 ± 0.049	0.523PheTrp: 0.523 ± 0.022	0.658PheTyr: 0.658 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.952GlyAla: 10.952 ± 0.108	0.59GlyCys: 0.59 ± 0.023	5.104GlyAsp: 5.104 ± 0.071	4.824GlyGlu: 4.824 ± 0.062	3.417GlyPhe: 3.417 ± 0.057	7.772GlyGly: 7.772 ± 0.104	1.755GlyHis: 1.755 ± 0.042	5.168GlyIle: 5.168 ± 0.073	2.064GlyLys: 2.064 ± 0.041	8.836GlyLeu: 8.836 ± 0.09	2.165GlyMet: 2.165 ± 0.04	1.647GlyAsn: 1.647 ± 0.04	3.828GlyPro: 3.828 ± 0.058	2.454GlyGln: 2.454 ± 0.044	6.044GlyArg: 6.044 ± 0.068	5.209GlySer: 5.209 ± 0.073	5.529GlyThr: 5.529 ± 0.09	8.127GlyVal: 8.127 ± 0.088	1.706GlyTrp: 1.706 ± 0.043	2.246GlyTyr: 2.246 ± 0.045	0.001GlyXaa: 0.001 ± 0.001
His	2.385HisAla: 2.385 ± 0.049	0.107HisCys: 0.107 ± 0.01	1.269HisAsp: 1.269 ± 0.04	1.153HisGlu: 1.153 ± 0.031	0.59HisPhe: 0.59 ± 0.02	2.003HisGly: 2.003 ± 0.042	0.551HisHis: 0.551 ± 0.026	0.786HisIle: 0.786 ± 0.028	0.294HisLys: 0.294 ± 0.017	1.933HisLeu: 1.933 ± 0.04	0.328HisMet: 0.328 ± 0.016	0.331HisAsn: 0.331 ± 0.018	1.492HisPro: 1.492 ± 0.039	0.472HisGln: 0.472 ± 0.018	1.415HisArg: 1.415 ± 0.04	0.95HisSer: 0.95 ± 0.03	1.036HisThr: 1.036 ± 0.036	1.714HisVal: 1.714 ± 0.037	0.264HisTrp: 0.264 ± 0.016	0.419HisTyr: 0.419 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.668IleAla: 7.668 ± 0.094	0.248IleCys: 0.248 ± 0.013	3.668IleAsp: 3.668 ± 0.048	3.057IleGlu: 3.057 ± 0.059	1.34IlePhe: 1.34 ± 0.036	5.077IleGly: 5.077 ± 0.074	0.76IleHis: 0.76 ± 0.027	2.12IleIle: 2.12 ± 0.05	0.73IleLys: 0.73 ± 0.027	4.269IleLeu: 4.269 ± 0.073	0.696IleMet: 0.696 ± 0.025	0.988IleAsn: 0.988 ± 0.032	2.605IlePro: 2.605 ± 0.042	1.08IleGln: 1.08 ± 0.026	2.711IleArg: 2.711 ± 0.046	2.477IleSer: 2.477 ± 0.041	2.875IleThr: 2.875 ± 0.05	5.165IleVal: 5.165 ± 0.068	0.553IleTrp: 0.553 ± 0.023	0.841IleTyr: 0.841 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	2.251LysAla: 2.251 ± 0.047	0.07LysCys: 0.07 ± 0.008	1.075LysAsp: 1.075 ± 0.038	0.869LysGlu: 0.869 ± 0.03	0.477LysPhe: 0.477 ± 0.021	1.48LysGly: 1.48 ± 0.037	0.384LysHis: 0.384 ± 0.017	0.888LysIle: 0.888 ± 0.03	0.662LysLys: 0.662 ± 0.028	1.726LysLeu: 1.726 ± 0.043	0.395LysMet: 0.395 ± 0.018	0.472LysAsn: 0.472 ± 0.022	1.087LysPro: 1.087 ± 0.032	0.609LysGln: 0.609 ± 0.022	1.4LysArg: 1.4 ± 0.035	1.073LysSer: 1.073 ± 0.038	1.273LysThr: 1.273 ± 0.036	1.622LysVal: 1.622 ± 0.041	0.237LysTrp: 0.237 ± 0.014	0.451LysTyr: 0.451 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	14.419LeuAla: 14.419 ± 0.135	0.523LeuCys: 0.523 ± 0.02	6.465LeuAsp: 6.465 ± 0.083	5.293LeuGlu: 5.293 ± 0.073	2.961LeuPhe: 2.961 ± 0.059	9.15LeuGly: 9.15 ± 0.109	1.827LeuHis: 1.827 ± 0.042	4.892LeuIle: 4.892 ± 0.079	1.619LeuLys: 1.619 ± 0.042	9.91LeuLeu: 9.91 ± 0.131	1.767LeuMet: 1.767 ± 0.039	1.875LeuAsn: 1.875 ± 0.038	5.523LeuPro: 5.523 ± 0.064	2.593LeuGln: 2.593 ± 0.051	7.341LeuArg: 7.341 ± 0.091	5.892LeuSer: 5.892 ± 0.074	6.524LeuThr: 6.524 ± 0.073	9.201LeuVal: 9.201 ± 0.104	1.332LeuTrp: 1.332 ± 0.038	1.68LeuTyr: 1.68 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.176MetAla: 2.176 ± 0.042	0.108MetCys: 0.108 ± 0.009	0.914MetAsp: 0.914 ± 0.029	0.667MetGlu: 0.667 ± 0.026	0.584MetPhe: 0.584 ± 0.024	1.42MetGly: 1.42 ± 0.033	0.359MetHis: 0.359 ± 0.019	0.955MetIle: 0.955 ± 0.032	0.452MetLys: 0.452 ± 0.019	2.122MetLeu: 2.122 ± 0.047	0.361MetMet: 0.361 ± 0.022	0.502MetAsn: 0.502 ± 0.021	1.224MetPro: 1.224 ± 0.031	0.581MetGln: 0.581 ± 0.022	1.433MetArg: 1.433 ± 0.031	1.542MetSer: 1.542 ± 0.031	1.817MetThr: 1.817 ± 0.034	1.38MetVal: 1.38 ± 0.035	0.259MetTrp: 0.259 ± 0.016	0.331MetTyr: 0.331 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.478AsnAla: 2.478 ± 0.047	0.133AsnCys: 0.133 ± 0.013	1.207AsnAsp: 1.207 ± 0.037	0.976AsnGlu: 0.976 ± 0.028	0.615AsnPhe: 0.615 ± 0.025	1.889AsnGly: 1.889 ± 0.05	0.331AsnHis: 0.331 ± 0.018	0.906AsnIle: 0.906 ± 0.029	0.373AsnLys: 0.373 ± 0.019	1.907AsnLeu: 1.907 ± 0.043	0.337AsnMet: 0.337 ± 0.019	0.439AsnAsn: 0.439 ± 0.023	1.588AsnPro: 1.588 ± 0.04	0.483AsnGln: 0.483 ± 0.021	1.149AsnArg: 1.149 ± 0.033	1.027AsnSer: 1.027 ± 0.035	1.176AsnThr: 1.176 ± 0.04	1.661AsnVal: 1.661 ± 0.046	0.318AsnTrp: 0.318 ± 0.016	0.449AsnTyr: 0.449 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	7.153ProAla: 7.153 ± 0.099	0.194ProCys: 0.194 ± 0.013	3.933ProAsp: 3.933 ± 0.064	3.804ProGlu: 3.804 ± 0.063	1.796ProPhe: 1.796 ± 0.044	4.886ProGly: 4.886 ± 0.065	1.133ProHis: 1.133 ± 0.034	2.329ProIle: 2.329 ± 0.046	0.974ProLys: 0.974 ± 0.03	5.006ProLeu: 5.006 ± 0.073	0.996ProMet: 0.996 ± 0.028	1.017ProAsn: 1.017 ± 0.031	2.411ProPro: 2.411 ± 0.064	1.56ProGln: 1.56 ± 0.042	3.393ProArg: 3.393 ± 0.062	3.076ProSer: 3.076 ± 0.057	3.561ProThr: 3.561 ± 0.071	4.901ProVal: 4.901 ± 0.071	0.905ProTrp: 0.905 ± 0.032	1.092ProTyr: 1.092 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.414GlnAla: 3.414 ± 0.063	0.113GlnCys: 0.113 ± 0.009	1.367GlnAsp: 1.367 ± 0.038	1.262GlnGlu: 1.262 ± 0.03	0.893GlnPhe: 0.893 ± 0.027	2.063GlnGly: 2.063 ± 0.041	0.616GlnHis: 0.616 ± 0.019	1.392GlnIle: 1.392 ± 0.03	0.596GlnLys: 0.596 ± 0.023	3.108GlnLeu: 3.108 ± 0.056	0.54GlnMet: 0.54 ± 0.021	0.649GlnAsn: 0.649 ± 0.022	1.434GlnPro: 1.434 ± 0.039	1.109GlnGln: 1.109 ± 0.04	2.321GlnArg: 2.321 ± 0.042	1.568GlnSer: 1.568 ± 0.036	1.578GlnThr: 1.578 ± 0.037	2.292GlnVal: 2.292 ± 0.042	0.528GlnTrp: 0.528 ± 0.024	0.603GlnTyr: 0.603 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	8.737ArgAla: 8.737 ± 0.112	0.295ArgCys: 0.295 ± 0.016	4.251ArgAsp: 4.251 ± 0.073	4.142ArgGlu: 4.142 ± 0.072	2.406ArgPhe: 2.406 ± 0.046	5.4ArgGly: 5.4 ± 0.07	1.466ArgHis: 1.466 ± 0.039	3.69ArgIle: 3.69 ± 0.052	1.24ArgLys: 1.24 ± 0.035	7.073ArgLeu: 7.073 ± 0.094	1.73ArgMet: 1.73 ± 0.037	1.29ArgAsn: 1.29 ± 0.037	3.427ArgPro: 3.427 ± 0.061	1.881ArgGln: 1.881 ± 0.041	6.018ArgArg: 6.018 ± 0.09	3.876ArgSer: 3.876 ± 0.061	4.204ArgThr: 4.204 ± 0.062	6.025ArgVal: 6.025 ± 0.075	1.132ArgTrp: 1.132 ± 0.033	1.497ArgTyr: 1.497 ± 0.037	0.001ArgXaa: 0.001 ± 0.001
Ser	7.198SerAla: 7.198 ± 0.095	0.283SerCys: 0.283 ± 0.017	3.325SerAsp: 3.325 ± 0.062	2.683SerGlu: 2.683 ± 0.047	1.998SerPhe: 1.998 ± 0.041	5.796SerGly: 5.796 ± 0.074	1.018SerHis: 1.018 ± 0.033	2.811SerIle: 2.811 ± 0.049	0.96SerLys: 0.96 ± 0.03	5.432SerLeu: 5.432 ± 0.069	1.217SerMet: 1.217 ± 0.031	1.035SerAsn: 1.035 ± 0.032	3.129SerPro: 3.129 ± 0.058	1.368SerGln: 1.368 ± 0.032	3.579SerArg: 3.579 ± 0.059	3.296SerSer: 3.296 ± 0.055	3.658SerThr: 3.658 ± 0.06	4.851SerVal: 4.851 ± 0.065	0.948SerTrp: 0.948 ± 0.031	1.122SerTyr: 1.122 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	8.022ThrAla: 8.022 ± 0.085	0.309ThrCys: 0.309 ± 0.019	3.923ThrAsp: 3.923 ± 0.063	3.02ThrGlu: 3.02 ± 0.049	1.956ThrPhe: 1.956 ± 0.042	5.822ThrGly: 5.822 ± 0.077	1.244ThrHis: 1.244 ± 0.03	3.105ThrIle: 3.105 ± 0.055	1.113ThrLys: 1.113 ± 0.034	6.155ThrLeu: 6.155 ± 0.073	1.047ThrMet: 1.047 ± 0.031	1.243ThrAsn: 1.243 ± 0.046	4.117ThrPro: 4.117 ± 0.075	1.552ThrGln: 1.552 ± 0.039	3.756ThrArg: 3.756 ± 0.056	3.363ThrSer: 3.363 ± 0.054	3.969ThrThr: 3.969 ± 0.075	6.006ThrVal: 6.006 ± 0.091	0.932ThrTrp: 0.932 ± 0.031	1.197ThrTyr: 1.197 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	11.917ValAla: 11.917 ± 0.126	0.52ValCys: 0.52 ± 0.022	5.682ValAsp: 5.682 ± 0.076	4.886ValGlu: 4.886 ± 0.069	2.932ValPhe: 2.932 ± 0.05	7.362ValGly: 7.362 ± 0.082	1.761ValHis: 1.761 ± 0.04	4.8ValIle: 4.8 ± 0.072	1.493ValLys: 1.493 ± 0.042	9.336ValLeu: 9.336 ± 0.108	1.594ValMet: 1.594 ± 0.038	1.814ValAsn: 1.814 ± 0.043	4.798ValPro: 4.798 ± 0.066	2.308ValGln: 2.308 ± 0.044	6.126ValArg: 6.126 ± 0.076	4.922ValSer: 4.922 ± 0.071	6.0ValThr: 6.0 ± 0.086	8.923ValVal: 8.923 ± 0.1	1.226ValTrp: 1.226 ± 0.033	1.6ValTyr: 1.6 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.657TrpAla: 1.657 ± 0.041	0.089TrpCys: 0.089 ± 0.01	0.861TrpAsp: 0.861 ± 0.03	0.639TrpGlu: 0.639 ± 0.022	0.602TrpPhe: 0.602 ± 0.026	1.217TrpGly: 1.217 ± 0.035	0.313TrpHis: 0.313 ± 0.018	0.831TrpIle: 0.831 ± 0.026	0.285TrpLys: 0.285 ± 0.016	1.746TrpLeu: 1.746 ± 0.045	0.4TrpMet: 0.4 ± 0.019	0.413TrpAsn: 0.413 ± 0.019	0.797TrpPro: 0.797 ± 0.026	0.529TrpGln: 0.529 ± 0.021	1.253TrpArg: 1.253 ± 0.032	0.974TrpSer: 0.974 ± 0.036	0.996TrpThr: 0.996 ± 0.033	1.223TrpVal: 1.223 ± 0.033	0.386TrpTrp: 0.386 ± 0.02	0.28TrpTyr: 0.28 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.492TyrAla: 2.492 ± 0.044	0.121TyrCys: 0.121 ± 0.009	1.319TyrAsp: 1.319 ± 0.033	1.104TyrGlu: 1.104 ± 0.03	0.725TyrPhe: 0.725 ± 0.026	1.933TyrGly: 1.933 ± 0.042	0.294TyrHis: 0.294 ± 0.017	0.814TyrIle: 0.814 ± 0.027	0.317TyrLys: 0.317 ± 0.018	2.085TyrLeu: 2.085 ± 0.044	0.28TyrMet: 0.28 ± 0.015	0.474TyrAsn: 0.474 ± 0.023	1.084TyrPro: 1.084 ± 0.032	0.545TyrGln: 0.545 ± 0.021	1.624TyrArg: 1.624 ± 0.035	1.129TyrSer: 1.129 ± 0.03	1.178TyrThr: 1.178 ± 0.034	1.639TyrVal: 1.639 ± 0.035	0.32TyrTrp: 0.32 ± 0.015	0.438TyrTyr: 0.438 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3629 proteins (1208867 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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