Corynebacterium phage phi674

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ikedavirus; Corynebacterium virus phi674

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PIZ1|A0A2H4PIZ1_9CAUD Uncharacterized protein OS=Corynebacterium phage phi674 OX=2052822 GN=phi674_gp32 PE=4 SV=1
MM1 pKa = 7.57IIVEE5 pKa = 4.71LVLTAYY11 pKa = 8.44VTIVLGTAGFIEE23 pKa = 5.25SVTGLYY29 pKa = 8.81PAQQLDD35 pKa = 4.27LVQQHH40 pKa = 6.27YY41 pKa = 10.34GLPSSYY47 pKa = 11.09GVMDD51 pKa = 4.55TIGAVISSS59 pKa = 3.8

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PJ61|A0A2H4PJ61_9CAUD Putative head-to-tail connector OS=Corynebacterium phage phi674 OX=2052822 GN=phi674_gp07 PE=4 SV=1
MM1 pKa = 7.61ARR3 pKa = 11.84GQGTQAQLALADD15 pKa = 4.11TLVQDD20 pKa = 4.87LARR23 pKa = 11.84LMRR26 pKa = 11.84GTPVDD31 pKa = 3.7RR32 pKa = 11.84VDD34 pKa = 3.07VASRR38 pKa = 11.84IEE40 pKa = 4.07HH41 pKa = 5.32YY42 pKa = 10.12RR43 pKa = 11.84ALRR46 pKa = 11.84TPRR49 pKa = 11.84YY50 pKa = 8.0EE51 pKa = 3.91SKK53 pKa = 10.91YY54 pKa = 9.2PKK56 pKa = 10.1KK57 pKa = 10.75

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13581

51

1572

251.5

27.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.881 ± 0.474

0.626 ± 0.093

6.745 ± 0.204

5.868 ± 0.242

2.584 ± 0.151

8.254 ± 0.475

2.327 ± 0.284

4.823 ± 0.208

4.123 ± 0.246

8.114 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.842 ± 0.152

3.608 ± 0.278

5.154 ± 0.293

3.814 ± 0.176

5.412 ± 0.387

6.391 ± 0.268

7.164 ± 0.33

7.393 ± 0.271

2.003 ± 0.211

2.872 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski