Corynebacterium phage phi674
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4PIZ1|A0A2H4PIZ1_9CAUD Uncharacterized protein OS=Corynebacterium phage phi674 OX=2052822 GN=phi674_gp32 PE=4 SV=1
MM1 pKa = 7.57 IIVEE5 pKa = 4.71 LVLTAYY11 pKa = 8.44 VTIVLGTAGFIEE23 pKa = 5.25 SVTGLYY29 pKa = 8.81 PAQQLDD35 pKa = 4.27 LVQQHH40 pKa = 6.27 YY41 pKa = 10.34 GLPSSYY47 pKa = 11.09 GVMDD51 pKa = 4.55 TIGAVISSS59 pKa = 3.8
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.979
IPC2_protein 4.101
IPC_protein 3.694
Toseland 3.579
ProMoST 3.948
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.528
Solomon 3.63
Lehninger 3.579
Nozaki 3.923
DTASelect 3.986
Thurlkill 3.706
EMBOSS 3.732
Sillero 3.846
Patrickios 0.006
IPC_peptide 3.63
IPC2_peptide 3.795
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|A0A2H4PJ61|A0A2H4PJ61_9CAUD Putative head-to-tail connector OS=Corynebacterium phage phi674 OX=2052822 GN=phi674_gp07 PE=4 SV=1
MM1 pKa = 7.61 ARR3 pKa = 11.84 GQGTQAQLALADD15 pKa = 4.11 TLVQDD20 pKa = 4.87 LARR23 pKa = 11.84 LMRR26 pKa = 11.84 GTPVDD31 pKa = 3.7 RR32 pKa = 11.84 VDD34 pKa = 3.07 VASRR38 pKa = 11.84 IEE40 pKa = 4.07 HH41 pKa = 5.32 YY42 pKa = 10.12 RR43 pKa = 11.84 ALRR46 pKa = 11.84 TPRR49 pKa = 11.84 YY50 pKa = 8.0 EE51 pKa = 3.91 SKK53 pKa = 10.91 YY54 pKa = 9.2 PKK56 pKa = 10.1 KK57 pKa = 10.75
Molecular weight: 6.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.677
IPC_protein 10.452
Toseland 10.35
ProMoST 10.233
Dawson 10.526
Bjellqvist 10.262
Wikipedia 10.76
Rodwell 10.716
Grimsley 10.613
Solomon 10.613
Lehninger 10.57
Nozaki 10.306
DTASelect 10.262
Thurlkill 10.394
EMBOSS 10.745
Sillero 10.452
Patrickios 10.496
IPC_peptide 10.613
IPC2_peptide 9.077
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
13581
51
1572
251.5
27.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.881 ± 0.474
0.626 ± 0.093
6.745 ± 0.204
5.868 ± 0.242
2.584 ± 0.151
8.254 ± 0.475
2.327 ± 0.284
4.823 ± 0.208
4.123 ± 0.246
8.114 ± 0.23
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.842 ± 0.152
3.608 ± 0.278
5.154 ± 0.293
3.814 ± 0.176
5.412 ± 0.387
6.391 ± 0.268
7.164 ± 0.33
7.393 ± 0.271
2.003 ± 0.211
2.872 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here