Scyliorhinus torazame (Cloudy catshark)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Chondrichthyes; Elasmobranchii; Selachii; Galeomorphii; Galeoidea; Carcharhiniformes; Scyliorhinidae; Scyliorhinus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27602 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A401PT58|A0A401PT58_SCYTO Plus3 domain-containing protein OS=Scyliorhinus torazame OX=75743 GN=scyTo_0015464 PE=4 SV=1
MM1 pKa = 7.07GTVFCWFDD9 pKa = 3.36GDD11 pKa = 4.19HH12 pKa = 5.92VLGMFHH18 pKa = 7.31GYY20 pKa = 9.54HH21 pKa = 5.39VLGMFDD27 pKa = 3.75GDD29 pKa = 3.79HH30 pKa = 6.0VLGVFDD36 pKa = 5.21GDD38 pKa = 3.93DD39 pKa = 3.43VLGVFDD45 pKa = 4.87GDD47 pKa = 3.79HH48 pKa = 5.87VLGVFDD54 pKa = 4.58GDD56 pKa = 3.53QVLGVFEE63 pKa = 4.86GDD65 pKa = 3.2HH66 pKa = 5.61VLGVFDD72 pKa = 4.72GDD74 pKa = 3.79HH75 pKa = 5.87VLGVFDD81 pKa = 4.72GDD83 pKa = 3.79HH84 pKa = 5.87VLGVFDD90 pKa = 4.6GDD92 pKa = 3.9HH93 pKa = 6.15VLGEE97 pKa = 4.12FDD99 pKa = 3.95GDD101 pKa = 4.19HH102 pKa = 6.52ILGVFDD108 pKa = 4.39GDD110 pKa = 3.7HH111 pKa = 5.69VLGVFEE117 pKa = 4.93GDD119 pKa = 3.2HH120 pKa = 5.61VLGVFDD126 pKa = 4.72GDD128 pKa = 3.79HH129 pKa = 5.87VLGVFDD135 pKa = 5.77GDD137 pKa = 3.56HH138 pKa = 6.95CLMGDD143 pKa = 3.47HH144 pKa = 6.33VLGVFDD150 pKa = 4.72GDD152 pKa = 4.0HH153 pKa = 5.92VLGVYY158 pKa = 10.57DD159 pKa = 4.04GDD161 pKa = 4.47HH162 pKa = 6.9ILRR165 pKa = 11.84VFDD168 pKa = 3.61GDD170 pKa = 3.62RR171 pKa = 11.84FLGVSDD177 pKa = 4.02GNHH180 pKa = 5.27VLGVFDD186 pKa = 4.09GDD188 pKa = 3.96YY189 pKa = 11.08VLGVFDD195 pKa = 4.73GNHH198 pKa = 5.33VLGVFDD204 pKa = 4.21GDD206 pKa = 3.37RR207 pKa = 11.84VLGVFDD213 pKa = 4.07WNHH216 pKa = 4.79VLRR219 pKa = 11.84VFDD222 pKa = 4.24GDD224 pKa = 3.77HH225 pKa = 5.82VLGVFDD231 pKa = 4.24GDD233 pKa = 3.29RR234 pKa = 11.84VLGVFHH240 pKa = 7.41GDD242 pKa = 3.01HH243 pKa = 6.09VLGVFDD249 pKa = 4.17GDD251 pKa = 3.96YY252 pKa = 11.08VLGVFDD258 pKa = 4.82GDD260 pKa = 3.79HH261 pKa = 5.87VLGVFDD267 pKa = 4.15GDD269 pKa = 3.97YY270 pKa = 10.43VLGMFDD276 pKa = 3.35VDD278 pKa = 3.49NVLGVFDD285 pKa = 4.12VDD287 pKa = 3.55HH288 pKa = 6.43VLGVFDD294 pKa = 4.24GDD296 pKa = 3.57RR297 pKa = 11.84VLGVYY302 pKa = 10.51DD303 pKa = 3.98GGHH306 pKa = 5.27VLGVFDD312 pKa = 3.96VDD314 pKa = 3.42RR315 pKa = 11.84VLGVFDD321 pKa = 4.79GDD323 pKa = 3.45HH324 pKa = 7.06DD325 pKa = 4.95LGVFDD330 pKa = 5.07GDD332 pKa = 3.73HH333 pKa = 5.87VLGVFDD339 pKa = 4.72GDD341 pKa = 3.79HH342 pKa = 5.87VLGVFDD348 pKa = 4.81GDD350 pKa = 3.94HH351 pKa = 5.92VLGGLMGTVFWEE363 pKa = 4.37CLMGTVFCWFDD374 pKa = 3.12GDD376 pKa = 4.24LVLGVFHH383 pKa = 7.34GDD385 pKa = 2.92HH386 pKa = 6.06VLGMFNGDD394 pKa = 3.05HH395 pKa = 5.97VLGVFDD401 pKa = 4.89GDD403 pKa = 4.35HH404 pKa = 6.41ILGVFDD410 pKa = 3.92GDD412 pKa = 3.47RR413 pKa = 11.84VLGVSDD419 pKa = 3.48RR420 pKa = 11.84TIVLWGPCAGWFDD433 pKa = 4.05GDD435 pKa = 3.87HH436 pKa = 5.9VLGVFDD442 pKa = 4.58GDD444 pKa = 3.63QVLGVFDD451 pKa = 4.94GDD453 pKa = 4.11DD454 pKa = 3.57VLGMFDD460 pKa = 3.86GDD462 pKa = 3.66HH463 pKa = 5.9VLGVFGGDD471 pKa = 2.92HH472 pKa = 5.83VLGVFDD478 pKa = 3.92GDD480 pKa = 4.18YY481 pKa = 11.11VHH483 pKa = 7.39GEE485 pKa = 3.75FDD487 pKa = 3.69GDD489 pKa = 3.81HH490 pKa = 5.99VLGVFDD496 pKa = 4.72GDD498 pKa = 3.79HH499 pKa = 5.87VLGVFDD505 pKa = 4.47GDD507 pKa = 3.87HH508 pKa = 5.7VVGVFDD514 pKa = 4.92GDD516 pKa = 3.99DD517 pKa = 3.48VLGMFDD523 pKa = 3.66GDD525 pKa = 3.36RR526 pKa = 11.84VLGVFDD532 pKa = 4.93GDD534 pKa = 5.17LILLVV539 pKa = 3.97

Molecular weight: 57.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A401Q8M1|A0A401Q8M1_SCYTO Uncharacterized protein (Fragment) OS=Scyliorhinus torazame OX=75743 GN=scyTo_0023172 PE=4 SV=1
MM1 pKa = 7.6SSGFRR6 pKa = 11.84LLPSGFRR13 pKa = 11.84LLPSRR18 pKa = 11.84FTVAIWIQTSAIRR31 pKa = 11.84IQTAVIRR38 pKa = 11.84IQTAVIRR45 pKa = 11.84IKK47 pKa = 10.01TAVTRR52 pKa = 11.84IQTAATRR59 pKa = 11.84IQSAAIRR66 pKa = 11.84IQTAVIRR73 pKa = 11.84IQTAVIRR80 pKa = 11.84IQIAVTRR87 pKa = 11.84IQTAATRR94 pKa = 11.84IQTVAIRR101 pKa = 11.84IQTVAIRR108 pKa = 11.84IQTAASWIQTAAIRR122 pKa = 11.84II123 pKa = 4.13

Molecular weight: 13.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27602

0

27602

10337261

30

21134

374.5

42.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.419 ± 0.021

2.199 ± 0.014

5.405 ± 0.019

7.401 ± 0.026

3.544 ± 0.012

6.018 ± 0.026

2.482 ± 0.009

5.088 ± 0.017

6.092 ± 0.02

9.376 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.375 ± 0.009

4.347 ± 0.013

5.002 ± 0.019

4.781 ± 0.018

5.477 ± 0.021

8.066 ± 0.023

5.68 ± 0.018

6.316 ± 0.019

1.138 ± 0.007

2.794 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski