Desulfofarcimen acetoxidans (strain ATCC 49208 / DSM 771 / KCTC 5769 / VKM B-1644 / 5575) (Desulfotomaculum acetoxidans)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfofarcimen; Desulfofarcimen acetoxidans

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4016 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8W198|C8W198_DESAS Uncharacterized protein OS=Desulfofarcimen acetoxidans (strain ATCC 49208 / DSM 771 / KCTC 5769 / VKM B-1644 / 5575) OX=485916 GN=Dtox_0623 PE=4 SV=1
MM1 pKa = 7.67SDD3 pKa = 2.91LRR5 pKa = 11.84SRR7 pKa = 11.84VAYY10 pKa = 10.06LQGLSEE16 pKa = 4.8GLDD19 pKa = 3.32LDD21 pKa = 4.35GASKK25 pKa = 10.34EE26 pKa = 4.32GKK28 pKa = 9.31VLQGIIDD35 pKa = 3.89VLEE38 pKa = 5.44DD39 pKa = 3.29IVDD42 pKa = 3.73TMDD45 pKa = 4.11VIEE48 pKa = 5.29DD49 pKa = 3.54SQEE52 pKa = 3.77QMEE55 pKa = 4.95EE56 pKa = 3.83YY57 pKa = 9.71MEE59 pKa = 6.08RR60 pKa = 11.84IDD62 pKa = 5.39EE63 pKa = 4.64DD64 pKa = 4.28LLSLEE69 pKa = 4.78DD70 pKa = 4.95DD71 pKa = 4.3YY72 pKa = 12.1YY73 pKa = 11.66DD74 pKa = 5.72DD75 pKa = 5.2RR76 pKa = 11.84SCTCEE81 pKa = 3.79HH82 pKa = 7.35DD83 pKa = 3.39GAEE86 pKa = 4.33YY87 pKa = 11.25VEE89 pKa = 4.73VDD91 pKa = 3.85CPEE94 pKa = 4.59CGEE97 pKa = 4.54TVCFDD102 pKa = 3.56SDD104 pKa = 3.36ILEE107 pKa = 4.73DD108 pKa = 3.76EE109 pKa = 4.61DD110 pKa = 4.93VIEE113 pKa = 4.54VTCPSCDD120 pKa = 2.79TVVFINDD127 pKa = 3.83DD128 pKa = 3.59QPLTGGNEE136 pKa = 4.13MIEE139 pKa = 3.95GHH141 pKa = 6.1SYY143 pKa = 10.71INKK146 pKa = 9.44KK147 pKa = 10.5SSDD150 pKa = 3.66III152 pKa = 3.96

Molecular weight: 17.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8W0A4|C8W0A4_DESAS Orotate phosphoribosyltransferase OS=Desulfofarcimen acetoxidans (strain ATCC 49208 / DSM 771 / KCTC 5769 / VKM B-1644 / 5575) OX=485916 GN=pyrE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.32QPNKK9 pKa = 8.28SKK11 pKa = 10.59RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.88GFLSRR21 pKa = 11.84MSTKK25 pKa = 10.33SGRR28 pKa = 11.84NVLKK32 pKa = 10.5NRR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.22GRR39 pKa = 11.84KK40 pKa = 8.93RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4016

0

4016

1201787

30

2786

299.2

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.515 ± 0.047

1.275 ± 0.018

5.11 ± 0.025

6.699 ± 0.047

3.963 ± 0.025

7.097 ± 0.052

1.738 ± 0.018

7.618 ± 0.043

6.7 ± 0.042

10.095 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.02

4.746 ± 0.034

3.812 ± 0.05

3.552 ± 0.026

4.753 ± 0.034

6.064 ± 0.032

5.106 ± 0.046

7.043 ± 0.048

0.994 ± 0.015

3.576 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski