Pseudomonas phage vB_PaeS_PAO1_Ab30

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Casadabanvirus; unclassified Casadabanvirus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1IVZ8|A0A0A1IVZ8_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeS_PAO1_Ab30 OX=1548918 GN=ORF22 PE=4 SV=1
MM1 pKa = 7.42AVYY4 pKa = 9.33TITLSDD10 pKa = 3.72TEE12 pKa = 4.2GGIDD16 pKa = 4.51FSMQGPPLHH25 pKa = 7.16DD26 pKa = 4.0SEE28 pKa = 4.7ASKK31 pKa = 10.46IAYY34 pKa = 10.23ALMQQ38 pKa = 4.28

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1IX72|A0A0A1IX72_9CAUD Putative capsid and scaffold protein OS=Pseudomonas phage vB_PaeS_PAO1_Ab30 OX=1548918 GN=ORF24 PE=4 SV=1
MM1 pKa = 7.2TNEE4 pKa = 4.2TKK6 pKa = 10.15TDD8 pKa = 3.35RR9 pKa = 11.84QRR11 pKa = 11.84RR12 pKa = 11.84LARR15 pKa = 11.84EE16 pKa = 3.61RR17 pKa = 11.84QRR19 pKa = 11.84AKK21 pKa = 10.65RR22 pKa = 11.84EE23 pKa = 3.43RR24 pKa = 11.84DD25 pKa = 3.22ALRR28 pKa = 11.84RR29 pKa = 11.84AALGGRR35 pKa = 11.84RR36 pKa = 11.84FNMDD40 pKa = 3.83MYY42 pKa = 10.99QGTADD47 pKa = 4.65ALDD50 pKa = 5.18LICAAGGFAEE60 pKa = 4.46PAEE63 pKa = 4.31AVTLLLHH70 pKa = 6.04NVAEE74 pKa = 4.23IAEE77 pKa = 4.21RR78 pKa = 11.84DD79 pKa = 3.33ASRR82 pKa = 11.84FAEE85 pKa = 4.92LIQKK89 pKa = 9.82RR90 pKa = 11.84NHH92 pKa = 6.39PGRR95 pKa = 11.84TKK97 pKa = 10.65RR98 pKa = 3.62

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11877

37

1160

201.3

22.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.916 ± 0.828

0.859 ± 0.133

5.902 ± 0.261

6.104 ± 0.289

2.711 ± 0.189

7.67 ± 0.328

1.701 ± 0.157

4.311 ± 0.171

3.25 ± 0.242

9.893 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.126

2.888 ± 0.222

4.976 ± 0.382

4.74 ± 0.257

7.771 ± 0.384

5.734 ± 0.263

5.481 ± 0.293

6.374 ± 0.239

1.718 ± 0.136

2.661 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski