Amino acid dipepetide frequency for Pseudomonas phage vB_PaeS_PAO1_Ab30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.777AlaAla: 18.777 ± 3.155
1.347AlaCys: 1.347 ± 0.393
7.999AlaAsp: 7.999 ± 0.878
8.926AlaGlu: 8.926 ± 1.401
3.116AlaPhe: 3.116 ± 0.516
9.347AlaGly: 9.347 ± 1.365
1.516AlaHis: 1.516 ± 0.313
6.652AlaIle: 6.652 ± 0.687
4.547AlaLys: 4.547 ± 0.659
13.894AlaLeu: 13.894 ± 1.295
3.368AlaMet: 3.368 ± 0.453
3.116AlaAsn: 3.116 ± 0.638
5.978AlaPro: 5.978 ± 0.724
5.473AlaGln: 5.473 ± 0.96
10.357AlaArg: 10.357 ± 1.113
6.989AlaSer: 6.989 ± 0.934
6.82AlaThr: 6.82 ± 0.616
5.894AlaVal: 5.894 ± 0.793
2.526AlaTrp: 2.526 ± 0.327
2.526AlaTyr: 2.526 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
0.926CysAla: 0.926 ± 0.273
0.084CysCys: 0.084 ± 0.072
0.758CysAsp: 0.758 ± 0.274
0.337CysGlu: 0.337 ± 0.222
0.421CysPhe: 0.421 ± 0.187
0.674CysGly: 0.674 ± 0.241
0.253CysHis: 0.253 ± 0.163
0.337CysIle: 0.337 ± 0.142
0.168CysLys: 0.168 ± 0.119
0.505CysLeu: 0.505 ± 0.193
0.084CysMet: 0.084 ± 0.116
0.253CysAsn: 0.253 ± 0.153
0.674CysPro: 0.674 ± 0.236
0.168CysGln: 0.168 ± 0.105
1.095CysArg: 1.095 ± 0.36
0.337CysSer: 0.337 ± 0.166
0.589CysThr: 0.589 ± 0.21
0.337CysVal: 0.337 ± 0.16
0.253CysTrp: 0.253 ± 0.149
0.253CysTyr: 0.253 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
7.494AspAla: 7.494 ± 0.929
0.253AspCys: 0.253 ± 0.163
3.2AspAsp: 3.2 ± 0.449
3.873AspGlu: 3.873 ± 0.618
1.516AspPhe: 1.516 ± 0.297
5.642AspGly: 5.642 ± 0.748
1.347AspHis: 1.347 ± 0.369
2.947AspIle: 2.947 ± 0.434
1.431AspLys: 1.431 ± 0.365
5.978AspLeu: 5.978 ± 0.754
1.937AspMet: 1.937 ± 0.426
1.431AspAsn: 1.431 ± 0.321
3.284AspPro: 3.284 ± 0.555
2.61AspGln: 2.61 ± 0.502
3.368AspArg: 3.368 ± 0.46
3.621AspSer: 3.621 ± 0.499
3.284AspThr: 3.284 ± 0.467
3.2AspVal: 3.2 ± 0.5
1.263AspTrp: 1.263 ± 0.296
1.347AspTyr: 1.347 ± 0.372
0.0AspXaa: 0.0 ± 0.0
Glu
7.831GluAla: 7.831 ± 0.639
0.758GluCys: 0.758 ± 0.289
2.947GluAsp: 2.947 ± 0.503
2.863GluGlu: 2.863 ± 0.63
1.852GluPhe: 1.852 ± 0.401
3.789GluGly: 3.789 ± 0.561
0.758GluHis: 0.758 ± 0.226
3.2GluIle: 3.2 ± 0.5
2.526GluLys: 2.526 ± 0.451
6.736GluLeu: 6.736 ± 0.638
1.6GluMet: 1.6 ± 0.399
2.105GluAsn: 2.105 ± 0.5
2.61GluPro: 2.61 ± 0.565
3.789GluGln: 3.789 ± 0.588
4.968GluArg: 4.968 ± 0.9
2.695GluSer: 2.695 ± 0.428
2.695GluThr: 2.695 ± 0.433
4.631GluVal: 4.631 ± 0.585
1.095GluTrp: 1.095 ± 0.286
1.6GluTyr: 1.6 ± 0.319
0.0GluXaa: 0.0 ± 0.0
Phe
3.116PheAla: 3.116 ± 0.628
0.421PheCys: 0.421 ± 0.167
2.273PheAsp: 2.273 ± 0.456
1.431PheGlu: 1.431 ± 0.379
0.758PhePhe: 0.758 ± 0.21
2.695PheGly: 2.695 ± 0.419
0.505PheHis: 0.505 ± 0.202
0.842PheIle: 0.842 ± 0.242
0.926PheLys: 0.926 ± 0.22
2.442PheLeu: 2.442 ± 0.491
0.926PheMet: 0.926 ± 0.265
1.179PheAsn: 1.179 ± 0.314
1.347PhePro: 1.347 ± 0.396
1.179PheGln: 1.179 ± 0.382
2.189PheArg: 2.189 ± 0.49
1.263PheSer: 1.263 ± 0.257
1.347PheThr: 1.347 ± 0.293
1.095PheVal: 1.095 ± 0.224
0.253PheTrp: 0.253 ± 0.139
0.926PheTyr: 0.926 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
7.073GlyAla: 7.073 ± 1.02
0.505GlyCys: 0.505 ± 0.184
3.789GlyAsp: 3.789 ± 0.799
4.463GlyGlu: 4.463 ± 0.591
3.2GlyPhe: 3.2 ± 0.415
6.652GlyGly: 6.652 ± 0.835
0.926GlyHis: 0.926 ± 0.238
3.452GlyIle: 3.452 ± 0.459
3.031GlyLys: 3.031 ± 0.577
7.241GlyLeu: 7.241 ± 0.975
1.431GlyMet: 1.431 ± 0.346
2.779GlyAsn: 2.779 ± 0.436
2.358GlyPro: 2.358 ± 0.383
4.968GlyGln: 4.968 ± 0.595
7.073GlyArg: 7.073 ± 0.691
5.389GlySer: 5.389 ± 0.835
3.452GlyThr: 3.452 ± 0.466
4.8GlyVal: 4.8 ± 0.689
1.6GlyTrp: 1.6 ± 0.385
2.526GlyTyr: 2.526 ± 0.62
0.0GlyXaa: 0.0 ± 0.0
His
1.937HisAla: 1.937 ± 0.414
0.084HisCys: 0.084 ± 0.076
0.926HisAsp: 0.926 ± 0.263
0.842HisGlu: 0.842 ± 0.34
0.421HisPhe: 0.421 ± 0.171
1.263HisGly: 1.263 ± 0.346
0.168HisHis: 0.168 ± 0.129
0.842HisIle: 0.842 ± 0.236
0.168HisLys: 0.168 ± 0.119
1.768HisLeu: 1.768 ± 0.317
0.926HisMet: 0.926 ± 0.303
0.842HisAsn: 0.842 ± 0.268
1.431HisPro: 1.431 ± 0.395
1.01HisGln: 1.01 ± 0.361
1.263HisArg: 1.263 ± 0.346
0.589HisSer: 0.589 ± 0.265
0.674HisThr: 0.674 ± 0.241
0.758HisVal: 0.758 ± 0.259
0.168HisTrp: 0.168 ± 0.108
0.926HisTyr: 0.926 ± 0.293
0.0HisXaa: 0.0 ± 0.0
Ile
4.8IleAla: 4.8 ± 0.777
0.589IleCys: 0.589 ± 0.227
3.958IleAsp: 3.958 ± 0.548
2.947IleGlu: 2.947 ± 0.444
0.589IlePhe: 0.589 ± 0.203
3.2IleGly: 3.2 ± 0.466
1.095IleHis: 1.095 ± 0.235
2.021IleIle: 2.021 ± 0.608
1.431IleLys: 1.431 ± 0.309
2.863IleLeu: 2.863 ± 0.399
0.674IleMet: 0.674 ± 0.178
1.095IleAsn: 1.095 ± 0.341
2.526IlePro: 2.526 ± 0.466
1.852IleGln: 1.852 ± 0.586
3.958IleArg: 3.958 ± 0.489
1.937IleSer: 1.937 ± 0.415
3.284IleThr: 3.284 ± 0.514
2.695IleVal: 2.695 ± 0.359
0.674IleTrp: 0.674 ± 0.244
0.926IleTyr: 0.926 ± 0.291
0.0IleXaa: 0.0 ± 0.0
Lys
5.136LysAla: 5.136 ± 0.729
0.168LysCys: 0.168 ± 0.126
0.758LysAsp: 0.758 ± 0.258
1.937LysGlu: 1.937 ± 0.474
0.589LysPhe: 0.589 ± 0.198
2.273LysGly: 2.273 ± 0.427
0.589LysHis: 0.589 ± 0.197
1.095LysIle: 1.095 ± 0.325
1.347LysLys: 1.347 ± 0.328
3.031LysLeu: 3.031 ± 0.45
0.505LysMet: 0.505 ± 0.218
1.01LysAsn: 1.01 ± 0.192
2.273LysPro: 2.273 ± 0.569
1.095LysGln: 1.095 ± 0.331
3.2LysArg: 3.2 ± 0.704
2.273LysSer: 2.273 ± 0.534
1.937LysThr: 1.937 ± 0.382
2.442LysVal: 2.442 ± 0.495
0.168LysTrp: 0.168 ± 0.105
0.674LysTyr: 0.674 ± 0.258
0.0LysXaa: 0.0 ± 0.0
Leu
15.578LeuAla: 15.578 ± 1.433
0.758LeuCys: 0.758 ± 0.249
6.736LeuAsp: 6.736 ± 0.623
6.989LeuGlu: 6.989 ± 0.845
2.358LeuPhe: 2.358 ± 0.539
7.41LeuGly: 7.41 ± 0.624
2.358LeuHis: 2.358 ± 0.505
3.537LeuIle: 3.537 ± 0.618
3.031LeuLys: 3.031 ± 0.566
9.347LeuLeu: 9.347 ± 0.973
2.021LeuMet: 2.021 ± 0.447
2.61LeuAsn: 2.61 ± 0.414
3.873LeuPro: 3.873 ± 0.554
3.452LeuGln: 3.452 ± 0.437
7.747LeuArg: 7.747 ± 0.727
4.8LeuSer: 4.8 ± 0.686
5.136LeuThr: 5.136 ± 0.827
7.578LeuVal: 7.578 ± 0.674
1.431LeuTrp: 1.431 ± 0.333
2.189LeuTyr: 2.189 ± 0.374
0.0LeuXaa: 0.0 ± 0.0
Met
4.126MetAla: 4.126 ± 0.477
0.084MetCys: 0.084 ± 0.088
2.273MetAsp: 2.273 ± 0.45
1.684MetGlu: 1.684 ± 0.363
0.421MetPhe: 0.421 ± 0.184
1.768MetGly: 1.768 ± 0.352
0.337MetHis: 0.337 ± 0.147
0.505MetIle: 0.505 ± 0.197
0.589MetLys: 0.589 ± 0.221
1.263MetLeu: 1.263 ± 0.329
0.505MetMet: 0.505 ± 0.229
0.505MetAsn: 0.505 ± 0.263
1.516MetPro: 1.516 ± 0.43
1.263MetGln: 1.263 ± 0.352
1.516MetArg: 1.516 ± 0.342
1.852MetSer: 1.852 ± 0.408
1.852MetThr: 1.852 ± 0.391
0.758MetVal: 0.758 ± 0.256
0.253MetTrp: 0.253 ± 0.187
0.337MetTyr: 0.337 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
3.031AsnAla: 3.031 ± 0.648
0.084AsnCys: 0.084 ± 0.083
1.263AsnAsp: 1.263 ± 0.331
1.347AsnGlu: 1.347 ± 0.308
0.674AsnPhe: 0.674 ± 0.335
3.116AsnGly: 3.116 ± 0.734
0.674AsnHis: 0.674 ± 0.227
0.758AsnIle: 0.758 ± 0.38
0.589AsnLys: 0.589 ± 0.259
3.2AsnLeu: 3.2 ± 0.565
0.926AsnMet: 0.926 ± 0.33
1.179AsnAsn: 1.179 ± 0.418
2.442AsnPro: 2.442 ± 0.537
1.095AsnGln: 1.095 ± 0.3
2.947AsnArg: 2.947 ± 0.432
1.684AsnSer: 1.684 ± 0.336
1.263AsnThr: 1.263 ± 0.278
1.263AsnVal: 1.263 ± 0.268
0.505AsnTrp: 0.505 ± 0.175
0.842AsnTyr: 0.842 ± 0.22
0.0AsnXaa: 0.0 ± 0.0
Pro
7.241ProAla: 7.241 ± 0.78
0.505ProCys: 0.505 ± 0.261
4.042ProAsp: 4.042 ± 0.619
2.779ProGlu: 2.779 ± 0.438
1.516ProPhe: 1.516 ± 0.377
3.789ProGly: 3.789 ± 0.495
0.842ProHis: 0.842 ± 0.421
1.937ProIle: 1.937 ± 0.329
1.516ProLys: 1.516 ± 0.379
4.463ProLeu: 4.463 ± 0.536
0.842ProMet: 0.842 ± 0.263
1.516ProAsn: 1.516 ± 0.38
2.273ProPro: 2.273 ± 0.553
2.273ProGln: 2.273 ± 0.448
3.116ProArg: 3.116 ± 0.562
3.537ProSer: 3.537 ± 0.427
2.695ProThr: 2.695 ± 0.542
3.284ProVal: 3.284 ± 0.548
0.421ProTrp: 0.421 ± 0.171
1.179ProTyr: 1.179 ± 0.351
0.0ProXaa: 0.0 ± 0.0
Gln
5.726GlnAla: 5.726 ± 1.112
0.253GlnCys: 0.253 ± 0.147
1.768GlnAsp: 1.768 ± 0.412
2.105GlnGlu: 2.105 ± 0.467
1.516GlnPhe: 1.516 ± 0.357
3.537GlnGly: 3.537 ± 0.522
1.095GlnHis: 1.095 ± 0.273
2.358GlnIle: 2.358 ± 0.373
1.179GlnLys: 1.179 ± 0.445
6.147GlnLeu: 6.147 ± 0.549
0.926GlnMet: 0.926 ± 0.381
1.347GlnAsn: 1.347 ± 0.336
2.442GlnPro: 2.442 ± 0.359
3.031GlnGln: 3.031 ± 0.739
3.621GlnArg: 3.621 ± 0.486
2.61GlnSer: 2.61 ± 0.448
2.105GlnThr: 2.105 ± 0.425
4.042GlnVal: 4.042 ± 0.513
0.842GlnTrp: 0.842 ± 0.296
0.758GlnTyr: 0.758 ± 0.283
0.0GlnXaa: 0.0 ± 0.0
Arg
8.673ArgAla: 8.673 ± 1.012
0.842ArgCys: 0.842 ± 0.3
4.547ArgAsp: 4.547 ± 0.532
5.305ArgGlu: 5.305 ± 0.746
2.021ArgPhe: 2.021 ± 0.523
4.463ArgGly: 4.463 ± 0.653
1.684ArgHis: 1.684 ± 0.336
3.621ArgIle: 3.621 ± 0.598
2.61ArgLys: 2.61 ± 0.444
8.168ArgLeu: 8.168 ± 0.795
1.347ArgMet: 1.347 ± 0.29
1.6ArgAsn: 1.6 ± 0.412
4.21ArgPro: 4.21 ± 0.898
4.968ArgGln: 4.968 ± 0.553
6.484ArgArg: 6.484 ± 0.89
3.705ArgSer: 3.705 ± 0.666
3.958ArgThr: 3.958 ± 0.564
4.884ArgVal: 4.884 ± 0.723
1.6ArgTrp: 1.6 ± 0.415
3.031ArgTyr: 3.031 ± 0.431
0.0ArgXaa: 0.0 ± 0.0
Ser
8.084SerAla: 8.084 ± 0.859
0.589SerCys: 0.589 ± 0.199
3.2SerAsp: 3.2 ± 0.592
3.284SerGlu: 3.284 ± 0.545
1.6SerPhe: 1.6 ± 0.297
4.21SerGly: 4.21 ± 0.671
0.926SerHis: 0.926 ± 0.287
2.442SerIle: 2.442 ± 0.475
2.105SerLys: 2.105 ± 0.318
6.063SerLeu: 6.063 ± 1.013
1.095SerMet: 1.095 ± 0.302
1.431SerAsn: 1.431 ± 0.334
3.116SerPro: 3.116 ± 0.599
2.273SerGln: 2.273 ± 0.399
3.621SerArg: 3.621 ± 0.642
3.873SerSer: 3.873 ± 0.655
3.368SerThr: 3.368 ± 0.562
3.284SerVal: 3.284 ± 0.479
1.263SerTrp: 1.263 ± 0.345
1.516SerTyr: 1.516 ± 0.377
0.0SerXaa: 0.0 ± 0.0
Thr
7.578ThrAla: 7.578 ± 1.063
0.337ThrCys: 0.337 ± 0.258
3.284ThrAsp: 3.284 ± 0.615
2.695ThrGlu: 2.695 ± 0.413
0.926ThrPhe: 0.926 ± 0.306
4.968ThrGly: 4.968 ± 0.905
0.674ThrHis: 0.674 ± 0.255
2.021ThrIle: 2.021 ± 0.435
1.768ThrLys: 1.768 ± 0.519
5.81ThrLeu: 5.81 ± 0.829
1.347ThrMet: 1.347 ± 0.348
1.937ThrAsn: 1.937 ± 0.35
1.937ThrPro: 1.937 ± 0.365
1.095ThrGln: 1.095 ± 0.249
3.116ThrArg: 3.116 ± 0.407
3.537ThrSer: 3.537 ± 0.593
3.789ThrThr: 3.789 ± 0.625
5.557ThrVal: 5.557 ± 0.819
0.842ThrTrp: 0.842 ± 0.264
1.6ThrTyr: 1.6 ± 0.287
0.0ThrXaa: 0.0 ± 0.0
Val
7.41ValAla: 7.41 ± 0.732
0.337ValCys: 0.337 ± 0.155
3.284ValAsp: 3.284 ± 0.404
5.052ValGlu: 5.052 ± 0.71
2.105ValPhe: 2.105 ± 0.424
4.379ValGly: 4.379 ± 0.678
0.674ValHis: 0.674 ± 0.185
2.442ValIle: 2.442 ± 0.441
2.105ValLys: 2.105 ± 0.4
5.557ValLeu: 5.557 ± 0.617
1.431ValMet: 1.431 ± 0.384
1.684ValAsn: 1.684 ± 0.324
2.863ValPro: 2.863 ± 0.432
3.368ValGln: 3.368 ± 0.484
4.8ValArg: 4.8 ± 0.655
4.042ValSer: 4.042 ± 0.582
4.294ValThr: 4.294 ± 0.716
3.958ValVal: 3.958 ± 0.612
1.179ValTrp: 1.179 ± 0.287
2.779ValTyr: 2.779 ± 0.614
0.0ValXaa: 0.0 ± 0.0
Trp
1.516TrpAla: 1.516 ± 0.315
0.337TrpCys: 0.337 ± 0.14
0.505TrpAsp: 0.505 ± 0.169
0.842TrpGlu: 0.842 ± 0.238
0.421TrpPhe: 0.421 ± 0.144
1.095TrpGly: 1.095 ± 0.333
0.084TrpHis: 0.084 ± 0.083
1.01TrpIle: 1.01 ± 0.191
0.842TrpLys: 0.842 ± 0.227
1.937TrpLeu: 1.937 ± 0.405
0.926TrpMet: 0.926 ± 0.345
0.421TrpAsn: 0.421 ± 0.179
0.842TrpPro: 0.842 ± 0.33
1.01TrpGln: 1.01 ± 0.188
1.347TrpArg: 1.347 ± 0.341
1.095TrpSer: 1.095 ± 0.249
0.926TrpThr: 0.926 ± 0.252
1.347TrpVal: 1.347 ± 0.367
0.505TrpTrp: 0.505 ± 0.185
0.168TrpTyr: 0.168 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.116TyrAla: 3.116 ± 0.524
0.253TyrCys: 0.253 ± 0.155
1.516TyrAsp: 1.516 ± 0.271
1.347TyrGlu: 1.347 ± 0.314
1.095TyrPhe: 1.095 ± 0.316
2.442TyrGly: 2.442 ± 0.468
0.505TyrHis: 0.505 ± 0.301
1.095TyrIle: 1.095 ± 0.261
0.589TyrLys: 0.589 ± 0.202
2.526TyrLeu: 2.526 ± 0.486
0.505TyrMet: 0.505 ± 0.215
0.842TyrAsn: 0.842 ± 0.238
1.768TyrPro: 1.768 ± 0.532
1.431TyrGln: 1.431 ± 0.333
1.937TyrArg: 1.937 ± 0.353
1.516TyrSer: 1.516 ± 0.397
1.347TyrThr: 1.347 ± 0.36
1.937TyrVal: 1.937 ± 0.436
0.337TyrTrp: 0.337 ± 0.191
0.505TyrTyr: 0.505 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (11877 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski