Streptococcus satellite phage Javan63
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZV35|A0A4D5ZV35_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan63 OX=2558794 GN=JavanS63_0006 PE=4 SV=1
MM1 pKa = 7.77 ADD3 pKa = 3.37 KK4 pKa = 11.27 KK5 pKa = 11.09 EE6 pKa = 3.65 FDD8 pKa = 4.13 LANEE12 pKa = 3.91 RR13 pKa = 11.84 AKK15 pKa = 11.2 NFGIWLEE22 pKa = 4.04 EE23 pKa = 4.37 SYY25 pKa = 8.94 QTMLDD30 pKa = 4.02 FSLEE34 pKa = 4.24 DD35 pKa = 4.04 KK36 pKa = 10.56 FDD38 pKa = 4.03 CYY40 pKa = 11.03 SIEE43 pKa = 4.08 EE44 pKa = 4.21 RR45 pKa = 11.84 NQLEE49 pKa = 4.34 RR50 pKa = 11.84 VLEE53 pKa = 4.07 TLMDD57 pKa = 4.18 FCDD60 pKa = 3.07 MWEE63 pKa = 4.02 RR64 pKa = 11.84 GQIILASEE72 pKa = 4.0 EE73 pKa = 4.36 RR74 pKa = 11.84 EE75 pKa = 3.88 MSKK78 pKa = 11.0
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.473
IPC2_protein 4.342
IPC_protein 4.228
Toseland 4.075
ProMoST 4.329
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.037
Rodwell 4.062
Grimsley 3.986
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.406
Thurlkill 4.075
EMBOSS 4.05
Sillero 4.329
Patrickios 3.897
IPC_peptide 4.164
IPC2_peptide 4.329
IPC2.peptide.svr19 4.251
Protein with the highest isoelectric point:
>tr|A0A4D5ZTH3|A0A4D5ZTH3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan63 OX=2558794 GN=JavanS63_0005 PE=4 SV=1
MM1 pKa = 7.61 SEE3 pKa = 4.16 LLGAILTLLLFFLAGICTQLFNAWAIAYY31 pKa = 7.92 KK32 pKa = 10.23 RR33 pKa = 11.84 RR34 pKa = 11.84 GYY36 pKa = 8.14 ITKK39 pKa = 9.71 KK40 pKa = 8.13 QLRR43 pKa = 11.84 KK44 pKa = 9.42 IEE46 pKa = 4.12 KK47 pKa = 9.79
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.214
IPC2_protein 9.589
IPC_protein 9.692
Toseland 10.365
ProMoST 10.058
Dawson 10.496
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 11.082
Grimsley 10.54
Solomon 10.54
Lehninger 10.526
Nozaki 10.35
DTASelect 10.116
Thurlkill 10.365
EMBOSS 10.745
Sillero 10.409
Patrickios 10.906
IPC_peptide 10.54
IPC2_peptide 8.873
IPC2.peptide.svr19 8.516
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2263
47
383
150.9
17.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.01 ± 0.641
0.707 ± 0.154
5.7 ± 0.448
8.617 ± 0.805
3.889 ± 0.264
4.861 ± 0.418
1.458 ± 0.383
7.512 ± 0.425
9.589 ± 0.3
10.119 ± 0.782
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.872 ± 0.532
5.524 ± 0.367
2.165 ± 0.289
4.596 ± 0.316
4.772 ± 0.55
5.479 ± 0.24
6.363 ± 0.698
4.684 ± 0.5
0.84 ± 0.119
4.242 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here