Corynebacterium matruchotii ATCC 14266
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2619 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0DH52|E0DH52_9CORY Heavy metal-associated domain protein OS=Corynebacterium matruchotii ATCC 14266 OX=553207 GN=HMPREF0299_5004 PE=4 SV=1
MM1 pKa = 7.83 AYY3 pKa = 10.23 SIYY6 pKa = 11.09 NFLRR10 pKa = 11.84 NTKK13 pKa = 9.98 EE14 pKa = 3.54 EE15 pKa = 4.22 TKK17 pKa = 10.9 ALDD20 pKa = 3.31 QMASYY25 pKa = 9.88 IHH27 pKa = 7.17 HH28 pKa = 5.6 NTDD31 pKa = 2.61 MKK33 pKa = 11.28 VYY35 pKa = 10.87 LFLTLEE41 pKa = 4.26 NYY43 pKa = 11.02 AEE45 pKa = 4.2 FEE47 pKa = 4.68 AIQEE51 pKa = 4.36 HH52 pKa = 5.08 YY53 pKa = 9.86 HH54 pKa = 6.19 GRR56 pKa = 11.84 VVWAVNAADD65 pKa = 3.9 TGPFGALDD73 pKa = 4.26 PYY75 pKa = 11.43 SFDD78 pKa = 3.68 GLVEE82 pKa = 4.2 FDD84 pKa = 4.84 GKK86 pKa = 10.63 DD87 pKa = 3.27 VDD89 pKa = 4.22 IDD91 pKa = 3.78 PGGAFYY97 pKa = 10.12 STDD100 pKa = 3.52 FSFDD104 pKa = 2.94 GLAIMADD111 pKa = 3.04 MVDD114 pKa = 4.04 FPEE117 pKa = 4.32 KK118 pKa = 10.12 VKK120 pKa = 10.79 EE121 pKa = 4.05 VLVSDD126 pKa = 4.19 AADD129 pKa = 3.73 FPEE132 pKa = 5.25 SFDD135 pKa = 5.32 DD136 pKa = 3.83 YY137 pKa = 11.65 LLMVQEE143 pKa = 4.78 QDD145 pKa = 4.58 DD146 pKa = 4.41 DD147 pKa = 4.43 PDD149 pKa = 5.71 PIEE152 pKa = 4.75 AKK154 pKa = 10.48 VLAAGALLVYY164 pKa = 10.27 SLNISDD170 pKa = 4.1 TVQGEE175 pKa = 4.67 TPGEE179 pKa = 4.08 LFLSEE184 pKa = 4.53 SVCIGDD190 pKa = 3.69 ARR192 pKa = 11.84 GEE194 pKa = 3.83 AFMYY198 pKa = 10.46 QFFDD202 pKa = 4.27 PNDD205 pKa = 3.58 ADD207 pKa = 3.81 RR208 pKa = 6.01
Molecular weight: 23.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.783
IPC_protein 3.795
Toseland 3.579
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.151
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.923
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|E0DHG3|E0DHG3_9CORY Uncharacterized protein OS=Corynebacterium matruchotii ATCC 14266 OX=553207 GN=HMPREF0299_5115 PE=4 SV=1
MM1 pKa = 7.46 TKK3 pKa = 10.27 GKK5 pKa = 8.73 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.01 HH19 pKa = 4.51 GFRR22 pKa = 11.84 IRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 SKK40 pKa = 10.76 GRR42 pKa = 11.84 AKK44 pKa = 9.67 LTAA47 pKa = 4.21
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2619
0
2619
820278
29
3044
313.2
34.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.937 ± 0.063
0.824 ± 0.015
5.942 ± 0.042
5.498 ± 0.043
3.333 ± 0.028
7.826 ± 0.048
2.361 ± 0.023
5.608 ± 0.04
3.552 ± 0.046
9.423 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.336 ± 0.022
3.245 ± 0.034
5.371 ± 0.041
3.611 ± 0.03
5.842 ± 0.047
5.733 ± 0.042
6.756 ± 0.043
7.934 ± 0.05
1.405 ± 0.019
2.463 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here