Circovirus-like genome DCCV-6
Average proteome isoelectric point is 8.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A190WHB0|A0A190WHB0_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-6 OX=1788446 PE=4 SV=1
MM1 pKa = 7.36 TGCPPQDD8 pKa = 3.18 VKK10 pKa = 11.37 ALSGSGRR17 pKa = 11.84 SQLISGDD24 pKa = 3.58 PLYY27 pKa = 10.46 TPKK30 pKa = 10.82 SPTLRR35 pKa = 11.84 DD36 pKa = 3.31 RR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 10.5 VGLTLDD45 pKa = 3.56 TCIGRR50 pKa = 11.84 SSSTSKK56 pKa = 10.8 RR57 pKa = 11.84 KK58 pKa = 10.19 DD59 pKa = 3.26 PLPLSRR65 pKa = 11.84 HH66 pKa = 5.62 AFLQRR71 pKa = 11.84 VTMNSPDD78 pKa = 3.78 QLPLKK83 pKa = 10.75 SMSLKK88 pKa = 9.63 RR89 pKa = 11.84 TLEE92 pKa = 4.03 SQTLSSSSEE101 pKa = 4.21 SNLSCEE107 pKa = 4.18 AEE109 pKa = 4.09 KK110 pKa = 10.85 QIGMKK115 pKa = 10.08 SGMLQEE121 pKa = 5.87 AEE123 pKa = 4.41 TSWLSLPMYY132 pKa = 9.88 EE133 pKa = 4.61 VLNFNQSNVILTSEE147 pKa = 4.35 EE148 pKa = 3.82 FAQILPNLSQWNVRR162 pKa = 11.84 FTYY165 pKa = 10.32 FGEE168 pKa = 4.18 IPVLEE173 pKa = 4.45 NPAGLGMRR181 pKa = 11.84 RR182 pKa = 11.84 VSALTLKK189 pKa = 10.89 VPVQNSGMVTKK200 pKa = 10.29 IKK202 pKa = 10.77 KK203 pKa = 8.7 MLLSMSLEE211 pKa = 4.09 EE212 pKa = 4.19 TSVILINKK220 pKa = 9.53 ASLTCSDD227 pKa = 3.81 GWTDD231 pKa = 2.92 IPFWSRR237 pKa = 11.84 QKK239 pKa = 11.08 DD240 pKa = 3.34 QGSYY244 pKa = 10.92 CLLAEE249 pKa = 4.41 SGSRR253 pKa = 11.84 VTSRR257 pKa = 11.84 LPTGILLWMRR267 pKa = 11.84 TLPLLCCEE275 pKa = 4.65 DD276 pKa = 3.51
Molecular weight: 30.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.845
IPC2_protein 8.024
IPC_protein 7.922
Toseland 7.614
ProMoST 8.448
Dawson 8.668
Bjellqvist 8.975
Wikipedia 8.595
Rodwell 8.712
Grimsley 7.454
Solomon 8.814
Lehninger 8.829
Nozaki 9.282
DTASelect 8.697
Thurlkill 8.785
EMBOSS 8.873
Sillero 9.107
Patrickios 4.825
IPC_peptide 8.814
IPC2_peptide 7.892
IPC2.peptide.svr19 7.983
Protein with the highest isoelectric point:
>tr|A0A190WHC4|A0A190WHC4_9CIRC Viral_Rep domain-containing protein OS=Circovirus-like genome DCCV-6 OX=1788446 PE=4 SV=1
MM1 pKa = 7.24 SVSRR5 pKa = 11.84 TPRR8 pKa = 11.84 SYY10 pKa = 11.28 GGQTLVGSRR19 pKa = 11.84 SRR21 pKa = 11.84 GLFRR25 pKa = 11.84 NTHH28 pKa = 5.38 NLAVGYY34 pKa = 9.5 GSRR37 pKa = 11.84 LLSAGIKK44 pKa = 10.14 RR45 pKa = 11.84 GAQHH49 pKa = 7.29 LMTKK53 pKa = 8.21 ITKK56 pKa = 9.36 KK57 pKa = 10.53 AKK59 pKa = 9.73 KK60 pKa = 9.91 ALAKK64 pKa = 10.53 KK65 pKa = 7.52 PTGYY69 pKa = 10.85 NEE71 pKa = 3.65 VSRR74 pKa = 11.84 SDD76 pKa = 3.27 RR77 pKa = 11.84 AAKK80 pKa = 10.02 VINRR84 pKa = 11.84 YY85 pKa = 7.78 NKK87 pKa = 9.35 KK88 pKa = 10.34 VSVKK92 pKa = 9.74 KK93 pKa = 8.45 PQRR96 pKa = 11.84 VKK98 pKa = 10.65 VSRR101 pKa = 11.84 KK102 pKa = 9.05 FKK104 pKa = 10.87 AKK106 pKa = 10.03 VNKK109 pKa = 10.08 ALEE112 pKa = 4.24 PQMSKK117 pKa = 10.6 GKK119 pKa = 10.47 YY120 pKa = 8.54 EE121 pKa = 3.92 EE122 pKa = 4.31 TSVFRR127 pKa = 11.84 QNFGIAFGNKK137 pKa = 8.89 QISGRR142 pKa = 11.84 LAPDD146 pKa = 3.5 TGAGSRR152 pKa = 11.84 HH153 pKa = 5.91 ALFGPAEE160 pKa = 4.18 ILDD163 pKa = 3.95 KK164 pKa = 11.18 ASCLFNNRR172 pKa = 11.84 VAGQTVATQTGNDD185 pKa = 3.73 TNFGNASLDD194 pKa = 3.32 PRR196 pKa = 11.84 TAKK199 pKa = 9.03 ITVNYY204 pKa = 10.14 QKK206 pKa = 10.58 YY207 pKa = 8.92 QCTLRR212 pKa = 11.84 NNSQRR217 pKa = 11.84 TWIIQIYY224 pKa = 7.85 EE225 pKa = 4.05 ASPKK229 pKa = 10.9 RR230 pKa = 11.84 MMNVCADD237 pKa = 4.04 ADD239 pKa = 3.91 VLSQWEE245 pKa = 4.08 SAMINEE251 pKa = 4.73 FNNDD255 pKa = 3.13 AGNAEE260 pKa = 4.23 LKK262 pKa = 10.55 KK263 pKa = 10.67 INLANATPNTLYY275 pKa = 9.38 ATPFMSKK282 pKa = 10.52 AFMKK286 pKa = 10.24 DD287 pKa = 2.86 WNIEE291 pKa = 3.8 KK292 pKa = 10.13 HH293 pKa = 7.13 DD294 pKa = 4.86 IILDD298 pKa = 3.73 PGQVYY303 pKa = 10.37 DD304 pKa = 3.86 HH305 pKa = 6.58 VMYY308 pKa = 10.77 GPTGTYY314 pKa = 10.44 DD315 pKa = 3.66 FSKK318 pKa = 10.04 FWRR321 pKa = 11.84 SDD323 pKa = 2.95 GVGASNEE330 pKa = 4.2 FYY332 pKa = 11.03 NLQPKK337 pKa = 7.5 FTRR340 pKa = 11.84 EE341 pKa = 3.63 LFYY344 pKa = 10.01 TARR347 pKa = 11.84 LDD349 pKa = 4.21 LCATDD354 pKa = 3.65 TGAVGRR360 pKa = 11.84 WGINDD365 pKa = 3.53 TTTGLIHH372 pKa = 7.12 EE373 pKa = 4.46 MTKK376 pKa = 8.92 TCSISVPQQAGGVLTEE392 pKa = 4.38 TGGLTAFRR400 pKa = 11.84 NTQQRR405 pKa = 11.84 DD406 pKa = 3.57 AYY408 pKa = 9.98 IKK410 pKa = 10.18 IHH412 pKa = 5.5 WNEE415 pKa = 3.64 NATAVPTVRR424 pKa = 11.84 VDD426 pKa = 3.24 VQTAGDD432 pKa = 3.72 NLL434 pKa = 3.87
Molecular weight: 48.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.109
IPC2_protein 9.268
IPC_protein 9.209
Toseland 10.014
ProMoST 9.663
Dawson 10.204
Bjellqvist 9.853
Wikipedia 10.35
Rodwell 10.672
Grimsley 10.262
Solomon 10.218
Lehninger 10.189
Nozaki 10.014
DTASelect 9.838
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.116
Patrickios 10.233
IPC_peptide 10.218
IPC2_peptide 8.448
IPC2.peptide.svr19 8.187
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
870
160
434
290.0
32.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.356 ± 1.677
1.609 ± 0.388
4.368 ± 0.292
4.483 ± 0.656
3.793 ± 0.681
7.126 ± 0.57
1.264 ± 0.359
4.483 ± 0.195
7.011 ± 0.672
8.391 ± 2.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.874 ± 0.471
5.287 ± 1.324
4.598 ± 0.788
4.368 ± 0.278
6.207 ± 0.177
8.391 ± 2.153
7.586 ± 0.618
5.862 ± 0.475
1.954 ± 0.443
2.989 ± 0.603
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here