Williamsia sterculiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Williamsia

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7GVV8|A0A1N7GVV8_9NOCA Glutamate-1-semialdehyde 2 1-aminomutase OS=Williamsia sterculiae OX=1344003 GN=SAMN05445060_3151 PE=3 SV=1
MM1 pKa = 7.24NFKK4 pKa = 10.71LYY6 pKa = 10.57RR7 pKa = 11.84CLQCGFEE14 pKa = 4.15YY15 pKa = 10.68DD16 pKa = 4.82EE17 pKa = 4.22EE18 pKa = 4.88LGWPEE23 pKa = 5.15DD24 pKa = 4.08GIEE27 pKa = 4.69PGTRR31 pKa = 11.84WDD33 pKa = 5.76DD34 pKa = 4.2IPDD37 pKa = 3.72DD38 pKa = 4.45WSCPDD43 pKa = 3.66CGAAKK48 pKa = 10.31SDD50 pKa = 3.62FEE52 pKa = 4.51MIEE55 pKa = 4.11VARR58 pKa = 11.84SS59 pKa = 3.08

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7FJ81|A0A1N7FJ81_9NOCA Adenosine kinase OS=Williamsia sterculiae OX=1344003 GN=SAMN05445060_2120 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.91GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4105

0

4105

1340898

30

11249

326.6

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.573 ± 0.051

0.721 ± 0.011

6.92 ± 0.041

4.87 ± 0.041

2.904 ± 0.02

9.052 ± 0.041

2.226 ± 0.019

4.212 ± 0.024

1.897 ± 0.029

9.572 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.902 ± 0.015

2.025 ± 0.022

5.534 ± 0.028

2.835 ± 0.023

7.68 ± 0.039

5.732 ± 0.03

6.633 ± 0.028

9.286 ± 0.04

1.441 ± 0.015

1.984 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski