Helicoverpa armigera (Cotton bollworm) (Heliothis armigera)
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8765 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W1BEC6|A0A2W1BEC6_HELAM Uncharacterized protein OS=Helicoverpa armigera OX=29058 GN=HaOG209482 PE=4 SV=1
MM1 pKa = 7.77 LIKK4 pKa = 10.23 LLCVVVIVGACVGEE18 pKa = 3.93 YY19 pKa = 10.57 VGDD22 pKa = 3.93 DD23 pKa = 3.85 EE24 pKa = 5.86 YY25 pKa = 12.06 VDD27 pKa = 4.71 AVLDD31 pKa = 3.63 MLLNGVSKK39 pKa = 10.56 EE40 pKa = 4.03 FEE42 pKa = 3.95 QSDD45 pKa = 3.94 EE46 pKa = 4.15 NDD48 pKa = 3.48 SGEE51 pKa = 4.26 VPVLTGGIWKK61 pKa = 10.05 CGRR64 pKa = 11.84 CDD66 pKa = 3.93 EE67 pKa = 4.39 IDD69 pKa = 3.69 EE70 pKa = 4.37 TRR72 pKa = 11.84 LIYY75 pKa = 10.76 SEE77 pKa = 4.26 VNEE80 pKa = 4.11 VDD82 pKa = 4.48 ADD84 pKa = 4.07 TLTDD88 pKa = 3.59 EE89 pKa = 5.09 SLDD92 pKa = 3.61 IQIGLSLPDD101 pKa = 3.68 MKK103 pKa = 10.75 CVTVNSDD110 pKa = 2.87 VAGVVRR116 pKa = 11.84 RR117 pKa = 11.84 VAGACSGEE125 pKa = 4.34 SVTLSVAPAQHH136 pKa = 5.77 FTVQVYY142 pKa = 8.46 SS143 pKa = 3.59
Molecular weight: 15.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.859
IPC_protein 3.821
Toseland 3.617
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.948
Patrickios 0.998
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A2W1BJ69|A0A2W1BJ69_HELAM Reverse transcriptase domain-containing protein OS=Helicoverpa armigera OX=29058 GN=HaOG207038 PE=4 SV=1
MM1 pKa = 7.42 RR2 pKa = 11.84 RR3 pKa = 11.84 HH4 pKa = 5.95 RR5 pKa = 11.84 ASALSRR11 pKa = 11.84 PRR13 pKa = 11.84 RR14 pKa = 11.84 PRR16 pKa = 11.84 TATRR20 pKa = 11.84 CRR22 pKa = 11.84 PQRR25 pKa = 11.84 AAGRR29 pKa = 11.84 LLRR32 pKa = 11.84 AAGGPQRR39 pKa = 11.84 VAGRR43 pKa = 11.84 PLRR46 pKa = 11.84 TAGRR50 pKa = 11.84 PQCAAGRR57 pKa = 11.84 PRR59 pKa = 11.84 PGGGRR64 pKa = 11.84 HH65 pKa = 5.61 PVPLL69 pKa = 4.75
Molecular weight: 7.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.439
IPC2_protein 10.95
IPC_protein 12.574
Toseland 12.72
ProMoST 13.232
Dawson 12.72
Bjellqvist 12.72
Wikipedia 13.203
Rodwell 12.223
Grimsley 12.764
Solomon 13.232
Lehninger 13.13
Nozaki 12.72
DTASelect 12.72
Thurlkill 12.72
EMBOSS 13.232
Sillero 12.72
Patrickios 11.959
IPC_peptide 13.232
IPC2_peptide 12.223
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8765
0
8765
3708793
36
9071
423.1
47.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.261 ± 0.041
2.017 ± 0.027
5.626 ± 0.024
6.56 ± 0.046
3.55 ± 0.022
5.544 ± 0.039
2.516 ± 0.017
5.195 ± 0.023
6.329 ± 0.046
8.945 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.289 ± 0.014
4.65 ± 0.024
5.561 ± 0.037
3.879 ± 0.025
5.756 ± 0.028
7.566 ± 0.036
5.859 ± 0.03
6.469 ± 0.026
1.121 ± 0.01
3.243 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here