Snakehead rhabdovirus (SHRV)
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9QJT9|NCAP_SHRV Nucleoprotein OS=Snakehead rhabdovirus OX=103603 GN=N PE=3 SV=1
MM1 pKa = 7.77 AFQKK5 pKa = 10.68 EE6 pKa = 4.4 FFGLRR11 pKa = 11.84 DD12 pKa = 3.59 VKK14 pKa = 11.12 VDD16 pKa = 3.78 LSAGEE21 pKa = 4.52 GLDD24 pKa = 3.67 FDD26 pKa = 4.51 PSEE29 pKa = 4.52 VEE31 pKa = 3.73 LTVYY35 pKa = 9.26 RR36 pKa = 11.84 TGADD40 pKa = 3.17 TDD42 pKa = 3.77 GNTIIKK48 pKa = 10.2 ALAAVGGPKK57 pKa = 9.72 TNEE60 pKa = 3.79 ALSVLLAFVTLGTDD74 pKa = 2.85 QDD76 pKa = 3.88 EE77 pKa = 4.66 YY78 pKa = 9.81 EE79 pKa = 4.19 TRR81 pKa = 11.84 IKK83 pKa = 10.12 ILKK86 pKa = 9.84 EE87 pKa = 3.14 IGFSVKK93 pKa = 9.34 EE94 pKa = 3.77 VPMAKK99 pKa = 9.87 DD100 pKa = 3.06 ASSGIEE106 pKa = 3.71 APLEE110 pKa = 3.88 NVAALVKK117 pKa = 10.53 PEE119 pKa = 3.92 TVYY122 pKa = 10.85 QVIRR126 pKa = 11.84 GVLYY130 pKa = 9.55 TCALFVKK137 pKa = 10.28 YY138 pKa = 10.15 NVEE141 pKa = 4.1 KK142 pKa = 8.09 MQKK145 pKa = 9.45 YY146 pKa = 9.75 IKK148 pKa = 10.5 NKK150 pKa = 10.08 LPALATSYY158 pKa = 10.53 GVPEE162 pKa = 4.58 LEE164 pKa = 4.23 EE165 pKa = 4.53 FPTEE169 pKa = 3.89 SSALKK174 pKa = 10.48 KK175 pKa = 10.17 LASCIRR181 pKa = 11.84 PGQRR185 pKa = 11.84 ITNALYY191 pKa = 10.7 AFLLVEE197 pKa = 4.28 MARR200 pKa = 11.84 PEE202 pKa = 4.25 TQQGARR208 pKa = 11.84 ALAAMRR214 pKa = 11.84 INGTGMTMVGLFTQAAKK231 pKa = 10.89 NLGATPSDD239 pKa = 3.95 LLEE242 pKa = 4.48 DD243 pKa = 3.49 LCMRR247 pKa = 11.84 SLVDD251 pKa = 3.14 SARR254 pKa = 11.84 RR255 pKa = 11.84 IVRR258 pKa = 11.84 LMVQVSQAEE267 pKa = 4.57 TIQARR272 pKa = 11.84 YY273 pKa = 9.15 AVMMSRR279 pKa = 11.84 MLNEE283 pKa = 4.63 NYY285 pKa = 9.88 FKK287 pKa = 10.9 AYY289 pKa = 9.95 GLNDD293 pKa = 3.3 NSRR296 pKa = 11.84 ISAILVAVNGHH307 pKa = 5.72 FSEE310 pKa = 4.47 DD311 pKa = 3.45 TIEE314 pKa = 4.05 ALEE317 pKa = 4.66 GIKK320 pKa = 10.6 VSAEE324 pKa = 3.8 FADD327 pKa = 4.08 LARR330 pKa = 11.84 RR331 pKa = 11.84 IAIALIEE338 pKa = 4.55 KK339 pKa = 9.91 YY340 pKa = 10.85 DD341 pKa = 3.68 NASDD345 pKa = 3.76 GGEE348 pKa = 4.04 GASEE352 pKa = 4.23 IIKK355 pKa = 10.32 SAVRR359 pKa = 11.84 GSSGAFKK366 pKa = 10.72 GSRR369 pKa = 11.84 GKK371 pKa = 9.6 PQGRR375 pKa = 11.84 RR376 pKa = 11.84 QEE378 pKa = 4.0 ASGEE382 pKa = 4.01 GDD384 pKa = 4.46 LDD386 pKa = 4.99 SEE388 pKa = 5.61 DD389 pKa = 6.32 DD390 pKa = 4.69 DD391 pKa = 4.84 DD392 pKa = 5.33 QDD394 pKa = 3.64 YY395 pKa = 11.68 SKK397 pKa = 11.44 YY398 pKa = 11.04 AA399 pKa = 3.36
Molecular weight: 43.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.001
IPC2_protein 5.029
IPC_protein 4.94
Toseland 4.8
ProMoST 5.029
Dawson 4.876
Bjellqvist 5.016
Wikipedia 4.724
Rodwell 4.787
Grimsley 4.711
Solomon 4.876
Lehninger 4.825
Nozaki 4.978
DTASelect 5.118
Thurlkill 4.787
EMBOSS 4.736
Sillero 5.054
Patrickios 4.24
IPC_peptide 4.876
IPC2_peptide 5.041
IPC2.peptide.svr19 5.089
Protein with the highest isoelectric point:
>sp|Q9QJT8|PHOSP_SHRV Phosphoprotein OS=Snakehead rhabdovirus OX=103603 GN=P PE=3 SV=1
MM1 pKa = 7.62 PFIRR5 pKa = 11.84 RR6 pKa = 11.84 PKK8 pKa = 9.64 RR9 pKa = 11.84 AILIPPLHH17 pKa = 6.52 LTLKK21 pKa = 10.75 DD22 pKa = 3.55 DD23 pKa = 4.57 DD24 pKa = 4.1 KK25 pKa = 11.56 VLVVEE30 pKa = 4.56 TVGTLIISGMTPSNLTEE47 pKa = 4.1 KK48 pKa = 10.74 LGLAMKK54 pKa = 9.94 LASAILGGDD63 pKa = 3.32 SHH65 pKa = 7.28 PAFNPLVEE73 pKa = 4.37 IFSGAMEE80 pKa = 4.72 FGASVEE86 pKa = 4.39 KK87 pKa = 10.73 LDD89 pKa = 4.89 FMTRR93 pKa = 11.84 EE94 pKa = 3.98 NKK96 pKa = 10.06 VITTYY101 pKa = 10.88 KK102 pKa = 8.52 VARR105 pKa = 11.84 GKK107 pKa = 10.31 AVALSNFPMEE117 pKa = 4.17 KK118 pKa = 9.55 RR119 pKa = 11.84 VGEE122 pKa = 4.07 KK123 pKa = 10.34 SYY125 pKa = 8.19 TTQIRR130 pKa = 11.84 NGSITYY136 pKa = 8.64 TGSFLFSAEE145 pKa = 4.04 HH146 pKa = 6.7 VGLKK150 pKa = 10.46 DD151 pKa = 3.55 NRR153 pKa = 11.84 SLFAAGEE160 pKa = 4.26 GLRR163 pKa = 11.84 EE164 pKa = 4.19 SPDD167 pKa = 3.08 MAQARR172 pKa = 11.84 EE173 pKa = 4.15 AFAGSLPKK181 pKa = 10.64 GKK183 pKa = 10.07 NEE185 pKa = 4.17 SSRR188 pKa = 11.84 KK189 pKa = 9.17
Molecular weight: 20.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.839
IPC2_protein 8.887
IPC_protein 8.829
Toseland 9.867
ProMoST 9.472
Dawson 9.999
Bjellqvist 9.575
Wikipedia 10.101
Rodwell 10.57
Grimsley 10.043
Solomon 10.043
Lehninger 10.014
Nozaki 9.809
DTASelect 9.589
Thurlkill 9.867
EMBOSS 10.233
Sillero 9.897
Patrickios 10.292
IPC_peptide 10.043
IPC2_peptide 7.922
IPC2.peptide.svr19 7.84
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3425
122
1983
570.8
64.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.511 ± 1.086
1.547 ± 0.353
5.343 ± 0.407
7.504 ± 0.561
3.474 ± 0.348
6.19 ± 0.51
2.277 ± 0.406
6.102 ± 0.206
6.657 ± 0.549
10.891 ± 1.35
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.408
3.533 ± 0.286
4.847 ± 0.753
3.299 ± 0.288
6.102 ± 0.512
7.62 ± 0.472
6.102 ± 0.385
5.752 ± 0.39
1.314 ± 0.358
2.686 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here