Quezon virus
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B0WVN1|A0A1B0WVN1_9VIRU Envelopment polyprotein OS=Quezon virus OX=1841195 PE=3 SV=1
MM1 pKa = 7.58 ASVKK5 pKa = 9.98 EE6 pKa = 4.1 LEE8 pKa = 4.45 EE9 pKa = 3.78 EE10 pKa = 3.89 QQRR13 pKa = 11.84 IEE15 pKa = 4.26 RR16 pKa = 11.84 EE17 pKa = 3.82 LSIAQAKK24 pKa = 8.16 LTDD27 pKa = 3.88 AKK29 pKa = 11.16 SKK31 pKa = 9.07 ITEE34 pKa = 3.92 SDD36 pKa = 3.38 KK37 pKa = 11.4 PDD39 pKa = 3.97 DD40 pKa = 5.14 LDD42 pKa = 3.53 IKK44 pKa = 10.18 MFNEE48 pKa = 3.81 RR49 pKa = 11.84 AEE51 pKa = 4.43 TVSNLRR57 pKa = 11.84 QRR59 pKa = 11.84 LEE61 pKa = 3.72 EE62 pKa = 4.19 TKK64 pKa = 10.88 RR65 pKa = 11.84 MIAEE69 pKa = 4.78 DD70 pKa = 3.12 IKK72 pKa = 11.24 KK73 pKa = 10.35 SVNQKK78 pKa = 7.8 PQKK81 pKa = 10.4 KK82 pKa = 8.03 GTDD85 pKa = 3.55 LDD87 pKa = 4.3 DD88 pKa = 5.96 DD89 pKa = 3.84 EE90 pKa = 6.32 HH91 pKa = 5.75 LTEE94 pKa = 4.96 RR95 pKa = 11.84 SSLRR99 pKa = 11.84 YY100 pKa = 10.39 GNVIDD105 pKa = 6.32 LNDD108 pKa = 3.65 TDD110 pKa = 3.93 IDD112 pKa = 4.1 EE113 pKa = 5.12 PSGSSADD120 pKa = 3.51 WISIVGYY127 pKa = 9.74 LLSFSLVIILKK138 pKa = 9.85 ALYY141 pKa = 9.36 MLTTRR146 pKa = 11.84 GRR148 pKa = 11.84 QTIKK152 pKa = 10.1 EE153 pKa = 4.12 NKK155 pKa = 8.49 GNRR158 pKa = 11.84 IRR160 pKa = 11.84 FKK162 pKa = 10.67 DD163 pKa = 3.52 DD164 pKa = 3.15 SSFTEE169 pKa = 3.82 KK170 pKa = 10.92 GGVKK174 pKa = 9.43 IPKK177 pKa = 8.92 HH178 pKa = 6.18 LYY180 pKa = 9.04 VSLPTGQSSMKK191 pKa = 10.18 ADD193 pKa = 4.65 EE194 pKa = 4.32 ITPGRR199 pKa = 11.84 YY200 pKa = 7.53 KK201 pKa = 10.36 TAVCGLYY208 pKa = 9.78 PAEE211 pKa = 4.18 AKK213 pKa = 9.88 ARR215 pKa = 11.84 KK216 pKa = 8.8 LVSPVMGVIGFNALVDD232 pKa = 3.45 KK233 pKa = 11.12 WEE235 pKa = 4.38 DD236 pKa = 3.68 TVSEE240 pKa = 4.39 MMLEE244 pKa = 4.17 PCQFIEE250 pKa = 4.74 GNLMSQPEE258 pKa = 4.63 DD259 pKa = 3.56 LTNAAYY265 pKa = 9.18 FAKK268 pKa = 10.41 RR269 pKa = 11.84 EE270 pKa = 3.81 EE271 pKa = 4.31 DD272 pKa = 3.35 LRR274 pKa = 11.84 GVRR277 pKa = 11.84 SPDD280 pKa = 3.01 MVQLYY285 pKa = 10.6 DD286 pKa = 3.6 LAKK289 pKa = 10.42 SAGCKK294 pKa = 9.39 LVNDD298 pKa = 3.74 ITEE301 pKa = 4.12 PHH303 pKa = 6.57 APWVFACAPDD313 pKa = 3.87 RR314 pKa = 11.84 CPPTALYY321 pKa = 10.59 VAGLAEE327 pKa = 4.62 LGAFFSILQDD337 pKa = 3.28 MRR339 pKa = 11.84 NTIMASKK346 pKa = 10.53 LVGTAEE352 pKa = 4.18 EE353 pKa = 4.06 KK354 pKa = 10.77 LKK356 pKa = 10.81 RR357 pKa = 11.84 KK358 pKa = 9.86 SSFYY362 pKa = 10.32 QSYY365 pKa = 10.61 LRR367 pKa = 11.84 RR368 pKa = 11.84 TQSMGIQLDD377 pKa = 3.23 QRR379 pKa = 11.84 IIVIFMLSWGKK390 pKa = 9.91 MMTDD394 pKa = 3.66 HH395 pKa = 6.84 FHH397 pKa = 7.53 LGDD400 pKa = 5.74 DD401 pKa = 3.57 MDD403 pKa = 6.0 ADD405 pKa = 4.04 LRR407 pKa = 11.84 KK408 pKa = 9.93 VCQSLIDD415 pKa = 3.96 EE416 pKa = 4.49 KK417 pKa = 11.32 VKK419 pKa = 10.87 QISNQDD425 pKa = 3.33 ALKK428 pKa = 10.82 LL429 pKa = 3.72
Molecular weight: 48.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.461
IPC2_protein 5.525
IPC_protein 5.499
Toseland 5.55
ProMoST 5.69
Dawson 5.563
Bjellqvist 5.652
Wikipedia 5.474
Rodwell 5.499
Grimsley 5.537
Solomon 5.563
Lehninger 5.525
Nozaki 5.728
DTASelect 5.893
Thurlkill 5.639
EMBOSS 5.614
Sillero 5.804
Patrickios 4.482
IPC_peptide 5.575
IPC2_peptide 5.779
IPC2.peptide.svr19 5.897
Protein with the highest isoelectric point:
>tr|A0A1B0WVN3|A0A1B0WVN3_9VIRU Replicase OS=Quezon virus OX=1841195 PE=4 SV=1
MM1 pKa = 7.97 RR2 pKa = 11.84 LILWMLILGFTDD14 pKa = 3.3 QKK16 pKa = 11.42 SLLEE20 pKa = 4.12 IKK22 pKa = 7.84 FTCPHH27 pKa = 6.33 DD28 pKa = 3.64 TRR30 pKa = 11.84 FEE32 pKa = 4.13 TGFGYY37 pKa = 10.7 GAVDD41 pKa = 4.12 LPSITEE47 pKa = 3.92 ASDD50 pKa = 4.47 LIVPVEE56 pKa = 4.16 TSCPFEE62 pKa = 4.16 FNLVRR67 pKa = 11.84 KK68 pKa = 9.21 QVTSVKK74 pKa = 9.5 MKK76 pKa = 7.34 WWEE79 pKa = 4.06 RR80 pKa = 11.84 KK81 pKa = 9.5 SSVAAGTNADD91 pKa = 3.37 QNTYY95 pKa = 8.2 EE96 pKa = 4.61 EE97 pKa = 4.5 KK98 pKa = 10.73 EE99 pKa = 4.03 KK100 pKa = 11.12 DD101 pKa = 2.97 AGAIGICNIQHH112 pKa = 6.59 NDD114 pKa = 2.7 ITSISQNRR122 pKa = 11.84 RR123 pKa = 11.84 GLMCLEE129 pKa = 4.11 YY130 pKa = 10.73 SCNLTHH136 pKa = 7.29 CMPALHH142 pKa = 6.64 VIIPHH147 pKa = 5.59 QICMNIRR154 pKa = 11.84 TCLLTWKK161 pKa = 8.59 STKK164 pKa = 9.44 IDD166 pKa = 4.5 LIFEE170 pKa = 5.1 RR171 pKa = 11.84 IFCPHH176 pKa = 7.37 GILVAGNCFQPYY188 pKa = 9.93 YY189 pKa = 10.61 GVTSPPPEE197 pKa = 4.01 SEE199 pKa = 3.96 RR200 pKa = 11.84 AEE202 pKa = 4.22 LSTTCFLITTKK213 pKa = 10.08 QSKK216 pKa = 10.28 AEE218 pKa = 4.05 KK219 pKa = 10.03 NLPQLVTAFQSEE231 pKa = 4.48 ITGNTCSPVNMAGYY245 pKa = 8.82 YY246 pKa = 9.31 SCFIKK251 pKa = 10.54 GYY253 pKa = 8.52 SQMINVPGHH262 pKa = 6.5 KK263 pKa = 10.04 DD264 pKa = 3.26 AITGEE269 pKa = 4.11 IMSKK273 pKa = 10.0 IIKK276 pKa = 9.7 FSHH279 pKa = 6.6 GEE281 pKa = 3.73 DD282 pKa = 3.17 HH283 pKa = 7.75 DD284 pKa = 5.31 RR285 pKa = 11.84 YY286 pKa = 8.72 TDD288 pKa = 3.16 GHH290 pKa = 6.79 GILNVAGPLNFSLKK304 pKa = 9.75 STDD307 pKa = 3.26 KK308 pKa = 10.97 FSGVCYY314 pKa = 10.52 SGDD317 pKa = 3.16 ILYY320 pKa = 9.64 TSLYY324 pKa = 9.68 MNTKK328 pKa = 10.23 KK329 pKa = 10.81 SGDD332 pKa = 3.74 DD333 pKa = 3.54 FTVVLAKK340 pKa = 10.71 GIIPNTDD347 pKa = 2.81 HH348 pKa = 7.19 SSCDD352 pKa = 3.5 MKK354 pKa = 11.12 LLPLVWQGMINIPGYY369 pKa = 8.89 IEE371 pKa = 4.6 KK372 pKa = 9.76 MEE374 pKa = 4.37 PCKK377 pKa = 10.77 VFCTLSGPGASCEE390 pKa = 4.26 AFSSTGIFNISSDD403 pKa = 3.18 TCLIGKK409 pKa = 7.18 LHH411 pKa = 7.03 RR412 pKa = 11.84 YY413 pKa = 8.4 KK414 pKa = 10.68 HH415 pKa = 6.44 LEE417 pKa = 3.87 DD418 pKa = 4.01 QISFICQRR426 pKa = 11.84 VDD428 pKa = 2.91 QDD430 pKa = 4.52 LVIYY434 pKa = 10.72 CNGQKK439 pKa = 10.31 KK440 pKa = 9.91 VIKK443 pKa = 9.84 TNTLVIGQCIYY454 pKa = 10.59 TITSALSLLPTIAHH468 pKa = 6.88 PLAVEE473 pKa = 4.12 LCVTGFHH480 pKa = 6.43 GWATLLLLITFCFGWLLIPLVSYY503 pKa = 9.85 LVIQVLRR510 pKa = 11.84 LILILMNKK518 pKa = 8.22 HH519 pKa = 5.44 TGQSRR524 pKa = 11.84 FEE526 pKa = 4.64 NILKK530 pKa = 10.21 KK531 pKa = 10.91 LKK533 pKa = 10.72 DD534 pKa = 3.87 EE535 pKa = 4.49 FQHH538 pKa = 6.43 TIGSTTCSYY547 pKa = 11.26 CKK549 pKa = 9.72 TDD551 pKa = 3.38 CCSKK555 pKa = 11.22 EE556 pKa = 3.81 EE557 pKa = 4.16 LTSHH561 pKa = 6.88 EE562 pKa = 4.59 EE563 pKa = 3.84 NCSKK567 pKa = 10.88 GKK569 pKa = 10.38 CPYY572 pKa = 9.2 CLKK575 pKa = 10.49 EE576 pKa = 4.27 IGSSSIIATEE586 pKa = 3.89 HH587 pKa = 6.48 FKK589 pKa = 10.31 ICPLIDD595 pKa = 3.41 RR596 pKa = 11.84 FITKK600 pKa = 9.6 IRR602 pKa = 11.84 QNVASTPTHH611 pKa = 5.12 GTLLYY616 pKa = 10.3 RR617 pKa = 11.84 KK618 pKa = 9.64 LGTFRR623 pKa = 11.84 YY624 pKa = 9.73 KK625 pKa = 10.39 NRR627 pKa = 11.84 CFIFTIWTVLIIIEE641 pKa = 4.28 SLVWAVSAQKK651 pKa = 8.3 MTVDD655 pKa = 4.58 PGWSDD660 pKa = 3.27 TAHH663 pKa = 6.4 GVGSVPMEE671 pKa = 4.44 LDD673 pKa = 3.46 YY674 pKa = 11.49 EE675 pKa = 4.51 LDD677 pKa = 3.5 FALVSGSTFIHH688 pKa = 6.42 KK689 pKa = 10.3 RR690 pKa = 11.84 LLQSPQAKK698 pKa = 9.1 EE699 pKa = 3.48 HH700 pKa = 6.43 TIPFTVTIDD709 pKa = 3.61 TQKK712 pKa = 9.06 ITAVVQTLGHH722 pKa = 6.04 WMDD725 pKa = 4.09 AEE727 pKa = 4.51 VNLKK731 pKa = 10.45 SVFHH735 pKa = 7.12 CYY737 pKa = 9.53 GACKK741 pKa = 9.77 AYY743 pKa = 9.79 KK744 pKa = 10.26 YY745 pKa = 9.08 PWQEE749 pKa = 3.92 AYY751 pKa = 10.33 CSHH754 pKa = 6.05 EE755 pKa = 3.63 VDD757 pKa = 4.58 YY758 pKa = 10.55 EE759 pKa = 4.32 YY760 pKa = 11.15 QSSWSCNPPTCPGISTGCTACGMYY784 pKa = 10.24 LDD786 pKa = 4.7 KK787 pKa = 10.4 LTPKK791 pKa = 10.5 ASVYY795 pKa = 10.55 KK796 pKa = 10.29 IINIKK801 pKa = 8.17 YY802 pKa = 9.64 SRR804 pKa = 11.84 YY805 pKa = 9.12 ICYY808 pKa = 10.05 QLGTEE813 pKa = 4.31 KK814 pKa = 10.34 QCKK817 pKa = 7.9 EE818 pKa = 4.05 VEE820 pKa = 4.3 GNDD823 pKa = 3.73 CVTGKK828 pKa = 9.79 HH829 pKa = 6.03 FKK831 pKa = 10.22 ACLVGTVSNIVASDD845 pKa = 3.46 TLVFLGPLDD854 pKa = 4.16 AGALIMKK861 pKa = 7.33 QWCVSNCKK869 pKa = 10.37 LGDD872 pKa = 3.93 PGDD875 pKa = 3.69 IMSVQDD881 pKa = 5.01 KK882 pKa = 10.26 INCPSYY888 pKa = 10.16 EE889 pKa = 3.85 GTINRR894 pKa = 11.84 VCRR897 pKa = 11.84 FATMPVCSYY906 pKa = 10.74 QGNQVPGFKK915 pKa = 10.69 KK916 pKa = 10.1 MMATKK921 pKa = 10.55 DD922 pKa = 3.38 SFLSVNMSNVKK933 pKa = 10.45 LSLSTMSWEE942 pKa = 4.94 DD943 pKa = 3.19 PDD945 pKa = 5.81 SIYY948 pKa = 10.62 KK949 pKa = 10.08 DD950 pKa = 3.54 HH951 pKa = 7.21 INIVVTKK958 pKa = 10.47 DD959 pKa = 3.13 LDD961 pKa = 3.85 FEE963 pKa = 4.39 EE964 pKa = 5.93 LSEE967 pKa = 4.31 NPCSVEE973 pKa = 4.1 SVISSIEE980 pKa = 4.03 GSWGSGVGFTITCSISLTEE999 pKa = 4.71 CSDD1002 pKa = 3.53 FLTTIKK1008 pKa = 10.86 ACDD1011 pKa = 3.44 DD1012 pKa = 4.57 AICYY1016 pKa = 9.23 GGKK1019 pKa = 9.86 AVRR1022 pKa = 11.84 LLRR1025 pKa = 11.84 GQNTVKK1031 pKa = 9.78 VQGRR1035 pKa = 11.84 GGHH1038 pKa = 5.75 SGSKK1042 pKa = 9.81 FRR1044 pKa = 11.84 CCHH1047 pKa = 5.97 EE1048 pKa = 4.22 NHH1050 pKa = 6.9 CSTKK1054 pKa = 10.32 GLRR1057 pKa = 11.84 ASSPHH1062 pKa = 6.62 LEE1064 pKa = 4.38 RR1065 pKa = 11.84 IGDD1068 pKa = 3.69 EE1069 pKa = 4.06 RR1070 pKa = 11.84 ALEE1073 pKa = 4.13 SQVFSDD1079 pKa = 4.87 GAPEE1083 pKa = 4.61 CGISCQFKK1091 pKa = 10.74 KK1092 pKa = 10.1 VWEE1095 pKa = 4.18 WLTGIFSGNWFILIVLVIVMIISIIILSFFCPAKK1129 pKa = 10.44 RR1130 pKa = 11.84 KK1131 pKa = 8.02 TSS1133 pKa = 3.25
Molecular weight: 126.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.433
IPC2_protein 6.605
IPC_protein 6.517
Toseland 6.427
ProMoST 7.059
Dawson 7.088
Bjellqvist 7.366
Wikipedia 7.029
Rodwell 7.102
Grimsley 6.478
Solomon 7.088
Lehninger 7.102
Nozaki 7.717
DTASelect 7.395
Thurlkill 7.439
EMBOSS 7.41
Sillero 7.614
Patrickios 3.261
IPC_peptide 7.088
IPC2_peptide 7.102
IPC2.peptide.svr19 6.902
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3709
429
2147
1236.3
140.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.311 ± 0.455
2.615 ± 1.039
5.797 ± 0.638
6.066 ± 0.443
4.691 ± 0.491
5.392 ± 0.601
2.453 ± 0.293
7.28 ± 0.493
7.468 ± 0.309
9.517 ± 0.451
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.723 ± 0.287
3.963 ± 0.29
3.667 ± 0.134
3.748 ± 0.183
3.99 ± 0.517
8.061 ± 0.261
5.851 ± 0.641
6.309 ± 0.283
1.456 ± 0.126
3.64 ± 0.358
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here