Pararhodospirillum photometricum DSM 122
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3275 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H6SS61|H6SS61_PARPM Uncharacterized protein OS=Pararhodospirillum photometricum DSM 122 OX=1150469 GN=RSPPHO_01114 PE=4 SV=1
MM1 pKa = 7.06 MPPYY5 pKa = 10.15 ALSASPMDD13 pKa = 4.02 PPEE16 pKa = 4.21 EE17 pKa = 4.84 GEE19 pKa = 4.27 VARR22 pKa = 11.84 CGTPLEE28 pKa = 4.84 PGTPAATVDD37 pKa = 3.51 AVIDD41 pKa = 3.93 VLRR44 pKa = 11.84 EE45 pKa = 3.75 IYY47 pKa = 10.59 DD48 pKa = 3.33 PEE50 pKa = 5.11 IPVNIYY56 pKa = 10.81 DD57 pKa = 4.37 LGLIYY62 pKa = 10.5 DD63 pKa = 4.09 VSVEE67 pKa = 4.08 ADD69 pKa = 3.87 GTCKK73 pKa = 10.26 ILMTLTAPACPVAGTLPPEE92 pKa = 3.95 MAQRR96 pKa = 11.84 VAAIPGVGEE105 pKa = 4.47 VIITLAWDD113 pKa = 4.08 PPWTPEE119 pKa = 4.0 RR120 pKa = 11.84 MSEE123 pKa = 4.24 VARR126 pKa = 11.84 VALDD130 pKa = 3.24 MFF132 pKa = 5.24
Molecular weight: 14.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.859
IPC_protein 3.783
Toseland 3.592
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.503
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.897
Patrickios 1.863
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|H6SKC7|H6SKC7_PARPM Cobyric acid synthase OS=Pararhodospirillum photometricum DSM 122 OX=1150469 GN=cobQ PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 5.32 GFLSRR21 pKa = 11.84 SATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.92 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3275
0
3275
1089982
40
3392
332.8
35.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.716 ± 0.064
0.96 ± 0.016
5.458 ± 0.036
5.542 ± 0.04
3.209 ± 0.027
8.872 ± 0.043
2.11 ± 0.019
3.974 ± 0.033
2.504 ± 0.029
11.252 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.202 ± 0.023
2.04 ± 0.026
6.046 ± 0.043
3.208 ± 0.031
8.048 ± 0.067
5.199 ± 0.042
5.649 ± 0.077
7.882 ± 0.037
1.322 ± 0.016
1.796 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here