Pararhodospirillum photometricum DSM 122

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Pararhodospirillum; Pararhodospirillum photometricum

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|H6SS61|H6SS61_PARPM Uncharacterized protein OS=Pararhodospirillum photometricum DSM 122 OX=1150469 GN=RSPPHO_01114 PE=4 SV=1
MM1 pKa = 7.06MPPYY5 pKa = 10.15ALSASPMDD13 pKa = 4.02PPEE16 pKa = 4.21EE17 pKa = 4.84GEE19 pKa = 4.27VARR22 pKa = 11.84CGTPLEE28 pKa = 4.84PGTPAATVDD37 pKa = 3.51AVIDD41 pKa = 3.93VLRR44 pKa = 11.84EE45 pKa = 3.75IYY47 pKa = 10.59DD48 pKa = 3.33PEE50 pKa = 5.11IPVNIYY56 pKa = 10.81DD57 pKa = 4.37LGLIYY62 pKa = 10.5DD63 pKa = 4.09VSVEE67 pKa = 4.08ADD69 pKa = 3.87GTCKK73 pKa = 10.26ILMTLTAPACPVAGTLPPEE92 pKa = 3.95MAQRR96 pKa = 11.84VAAIPGVGEE105 pKa = 4.47VIITLAWDD113 pKa = 4.08PPWTPEE119 pKa = 4.0RR120 pKa = 11.84MSEE123 pKa = 4.24VARR126 pKa = 11.84VALDD130 pKa = 3.24MFF132 pKa = 5.24

Molecular weight:
14.06 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|H6SKC7|H6SKC7_PARPM Cobyric acid synthase OS=Pararhodospirillum photometricum DSM 122 OX=1150469 GN=cobQ PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 5.32GFLSRR21 pKa = 11.84SATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.09 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3275

0

3275

1089982

40

3392

332.8

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.716 ± 0.064

0.96 ± 0.016

5.458 ± 0.036

5.542 ± 0.04

3.209 ± 0.027

8.872 ± 0.043

2.11 ± 0.019

3.974 ± 0.033

2.504 ± 0.029

11.252 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.023

2.04 ± 0.026

6.046 ± 0.043

3.208 ± 0.031

8.048 ± 0.067

5.199 ± 0.042

5.649 ± 0.077

7.882 ± 0.037

1.322 ± 0.016

1.796 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski