Joinjakaka virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Hapavirus; Joinjakaka hapavirus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R1D5|A0A0D3R1D5_9RHAB Uncharacterized protein OS=Joinjakaka virus OX=1272943 PE=4 SV=1
MM1 pKa = 8.02AYY3 pKa = 9.8QKK5 pKa = 10.17PKK7 pKa = 10.45KK8 pKa = 10.02FSSGVNWDD16 pKa = 3.8NLSKK20 pKa = 10.3SFKK23 pKa = 10.34MGDD26 pKa = 3.07HH27 pKa = 6.96DD28 pKa = 3.91EE29 pKa = 4.37EE30 pKa = 4.64VFEE33 pKa = 4.53EE34 pKa = 4.44EE35 pKa = 5.36VMTSLQIKK43 pKa = 10.14EE44 pKa = 4.34EE45 pKa = 4.39GEE47 pKa = 4.06QLDD50 pKa = 4.22DD51 pKa = 4.99LDD53 pKa = 3.79WGYY56 pKa = 11.51SSKK59 pKa = 11.08GDD61 pKa = 3.53KK62 pKa = 10.49EE63 pKa = 4.64YY64 pKa = 10.5IDD66 pKa = 5.03EE67 pKa = 4.56DD68 pKa = 4.04EE69 pKa = 4.93GWFSLPLEE77 pKa = 4.25TTPYY81 pKa = 10.6QLEE84 pKa = 4.34CMKK87 pKa = 10.76KK88 pKa = 8.31QVRR91 pKa = 11.84NVLRR95 pKa = 11.84KK96 pKa = 9.88NGLICLVEE104 pKa = 4.14VEE106 pKa = 4.62IKK108 pKa = 7.93TTASEE113 pKa = 4.61KK114 pKa = 10.25YY115 pKa = 10.15VKK117 pKa = 10.44LNCDD121 pKa = 3.05VAQRR125 pKa = 11.84TPVQPTEE132 pKa = 4.19VINFLKK138 pKa = 10.51NARR141 pKa = 11.84PDD143 pKa = 3.65NQSDD147 pKa = 3.81LSIKK151 pKa = 10.7KK152 pKa = 8.24NTTPQSGDD160 pKa = 3.04KK161 pKa = 11.23VMTGCLMGALQEE173 pKa = 4.66SVNQDD178 pKa = 3.38LEE180 pKa = 4.35KK181 pKa = 11.06GILLKK186 pKa = 10.68RR187 pKa = 11.84ITGNGSFTLTWDD199 pKa = 4.44HH200 pKa = 6.73INSNGGSLEE209 pKa = 4.05LLSNTKK215 pKa = 10.2GYY217 pKa = 10.59NKK219 pKa = 10.13HH220 pKa = 6.91DD221 pKa = 3.65VLEE224 pKa = 4.44NLLKK228 pKa = 10.9SIGLYY233 pKa = 9.49KK234 pKa = 10.63KK235 pKa = 7.83MWIFYY240 pKa = 10.09RR241 pKa = 11.84HH242 pKa = 5.84NLYY245 pKa = 10.7CC246 pKa = 5.07

Molecular weight: 28.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R1K1|A0A0D3R1K1_9RHAB Nucleocapsid protein OS=Joinjakaka virus OX=1272943 PE=4 SV=1
MM1 pKa = 7.46GMLTGVSLEE10 pKa = 4.11MFDD13 pKa = 3.7IRR15 pKa = 11.84DD16 pKa = 3.9RR17 pKa = 11.84FDD19 pKa = 3.1QLINIFKK26 pKa = 9.97EE27 pKa = 4.63FKK29 pKa = 10.11EE30 pKa = 3.94KK31 pKa = 11.31LEE33 pKa = 4.32VYY35 pKa = 11.02VNMLSSIFKK44 pKa = 10.55QILFYY49 pKa = 10.78ILVIVAVMVILKK61 pKa = 9.73LIIIVFKK68 pKa = 9.86TAILVIKK75 pKa = 9.51CYY77 pKa = 9.87RR78 pKa = 11.84VCKK81 pKa = 9.84CKK83 pKa = 10.31KK84 pKa = 9.05NKK86 pKa = 9.64SVHH89 pKa = 4.63VKK91 pKa = 10.1RR92 pKa = 11.84KK93 pKa = 6.91KK94 pKa = 8.61TSIISRR100 pKa = 11.84IRR102 pKa = 11.84QARR105 pKa = 11.84LQRR108 pKa = 11.84KK109 pKa = 6.24TRR111 pKa = 11.84LKK113 pKa = 10.18TKK115 pKa = 10.69LDD117 pKa = 3.68LL118 pKa = 4.67

Molecular weight: 14.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4236

118

2133

470.7

54.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.187 ± 0.444

1.771 ± 0.204

5.123 ± 0.37

6.091 ± 0.538

4.792 ± 0.281

5.406 ± 0.364

2.408 ± 0.262

7.483 ± 0.473

7.507 ± 0.472

10.316 ± 0.773

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.207

6.138 ± 0.293

4.202 ± 0.329

3.541 ± 0.186

4.108 ± 0.258

7.578 ± 0.359

5.453 ± 0.686

6.161 ± 0.569

2.077 ± 0.23

3.848 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski