Vibrio phage K04M1_VK04M1

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6TM40|A0A1W6TM40_9VIRU Putative minor coat protein OS=Vibrio phage K04M1_VK04M1 OX=1912325 GN=K04M1_52420 PE=4 SV=1
MM1 pKa = 6.89NHH3 pKa = 6.09YY4 pKa = 10.66LRR6 pKa = 11.84FFIALVILCASSHH19 pKa = 5.59TYY21 pKa = 10.64ALEE24 pKa = 3.66ARR26 pKa = 11.84IGHH29 pKa = 5.02MQMRR33 pKa = 11.84GCGSQGEE40 pKa = 4.46WVDD43 pKa = 3.61PYY45 pKa = 11.39KK46 pKa = 11.43VNTCFLDD53 pKa = 2.94TGYY56 pKa = 11.14FDD58 pKa = 3.34TCTFEE63 pKa = 3.83KK64 pKa = 10.29TPYY67 pKa = 10.41SNARR71 pKa = 11.84YY72 pKa = 8.73PYY74 pKa = 9.25QTVCDD79 pKa = 3.76NGLGLGYY86 pKa = 10.63YY87 pKa = 7.75EE88 pKa = 4.43VRR90 pKa = 11.84CPEE93 pKa = 3.81NSEE96 pKa = 4.27FDD98 pKa = 3.99PSTLRR103 pKa = 11.84CKK105 pKa = 10.33SVCEE109 pKa = 4.04YY110 pKa = 11.13GKK112 pKa = 10.26NPDD115 pKa = 3.94GTCMDD120 pKa = 3.88ACQFKK125 pKa = 10.92KK126 pKa = 10.76SIDD129 pKa = 4.02EE130 pKa = 4.15IKK132 pKa = 10.48SLQWLAYY139 pKa = 9.88VYY141 pKa = 10.64GEE143 pKa = 4.11QVTGSCYY150 pKa = 10.68GDD152 pKa = 3.56YY153 pKa = 11.09GATRR157 pKa = 11.84CEE159 pKa = 4.25LEE161 pKa = 4.18RR162 pKa = 11.84TPSDD166 pKa = 3.21STLCTGVDD174 pKa = 3.31SGQWTQNTICHH185 pKa = 6.32GNFQFTGNQCEE196 pKa = 4.28GGTLFWGKK204 pKa = 10.11DD205 pKa = 3.62GPDD208 pKa = 3.31TPIIPDD214 pKa = 4.37DD215 pKa = 5.14PIHH218 pKa = 7.61DD219 pKa = 4.88PDD221 pKa = 5.88DD222 pKa = 3.89PTGDD226 pKa = 3.64IEE228 pKa = 5.54DD229 pKa = 4.5PSILPDD235 pKa = 3.61GSTNTVNPPDD245 pKa = 4.02TDD247 pKa = 3.65SEE249 pKa = 4.63PDD251 pKa = 3.48VEE253 pKa = 5.55EE254 pKa = 5.22PDD256 pKa = 3.63TDD258 pKa = 3.49EE259 pKa = 4.45STDD262 pKa = 3.64TAVLKK267 pKa = 10.91AITGMNKK274 pKa = 10.0DD275 pKa = 3.38VNKK278 pKa = 10.54ALNDD282 pKa = 3.47MNIDD286 pKa = 3.4INQANADD293 pKa = 3.75VQNQIIALNASMVTNTQAIQKK314 pKa = 7.84QQINDD319 pKa = 3.14NKK321 pKa = 10.37IYY323 pKa = 10.49EE324 pKa = 4.16NTKK327 pKa = 10.95ALIQQANADD336 pKa = 3.49ITTAMNKK343 pKa = 7.52NTNAVNGVGDD353 pKa = 4.45DD354 pKa = 3.72VEE356 pKa = 5.55KK357 pKa = 10.74IAGAMDD363 pKa = 5.45GIAEE367 pKa = 4.26DD368 pKa = 3.65VSGISDD374 pKa = 3.9TLDD377 pKa = 4.1GIANTDD383 pKa = 3.08TSGAGTGGTCIEE395 pKa = 4.43SQSCTGFYY403 pKa = 10.69EE404 pKa = 4.04SGYY407 pKa = 9.68PDD409 pKa = 3.62GLGGLVSGQLDD420 pKa = 3.73DD421 pKa = 5.58LKK423 pKa = 11.35HH424 pKa = 4.84NTIDD428 pKa = 3.42NFVNSFGDD436 pKa = 3.73LDD438 pKa = 4.02LSSAKK443 pKa = 10.1RR444 pKa = 11.84PSFVLPVPFFGDD456 pKa = 3.76FSFEE460 pKa = 4.02EE461 pKa = 4.39QISFDD466 pKa = 3.32WVFGFIRR473 pKa = 11.84AVLIMTSVFAARR485 pKa = 11.84RR486 pKa = 11.84IIFGGG491 pKa = 3.39

Molecular weight: 53.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6TMA4|A0A1W6TMA4_9VIRU Uncharacterized protein OS=Vibrio phage K04M1_VK04M1 OX=1912325 GN=K04M1_52390 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.4RR3 pKa = 11.84LNALKK8 pKa = 10.52KK9 pKa = 10.17FGKK12 pKa = 9.82QAVATVTVAVLSVPAMAAEE31 pKa = 5.12GGAADD36 pKa = 4.32PFSVIDD42 pKa = 3.91LSGVATKK49 pKa = 10.3IGAAGLVIVGITMAYY64 pKa = 9.95KK65 pKa = 10.65SITLAKK71 pKa = 9.96RR72 pKa = 11.84AVNKK76 pKa = 10.37AA77 pKa = 3.05

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

2033

35

491

184.8

20.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.182 ± 1.082

1.968 ± 0.439

6.985 ± 0.83

5.214 ± 0.451

4.624 ± 0.297

7.329 ± 0.622

2.115 ± 0.428

6.149 ± 0.332

6.099 ± 0.837

8.903 ± 1.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.66

4.476 ± 0.673

4.181 ± 0.421

3.689 ± 0.411

4.132 ± 0.696

6.345 ± 0.54

6.149 ± 0.863

6.444 ± 0.627

1.426 ± 0.243

4.033 ± 0.476
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski