Streptomyces phage MulchMansion

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samistivirus; unclassified Samistivirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9W1N5|A0A7G9W1N5_9CAUD Uncharacterized protein OS=Streptomyces phage MulchMansion OX=2768141 GN=141 PE=4 SV=1
MM1 pKa = 7.15TNLYY5 pKa = 10.54GRR7 pKa = 11.84TLRR10 pKa = 11.84GEE12 pKa = 4.2PQDD15 pKa = 3.49NDD17 pKa = 3.07SWPEE21 pKa = 3.84QEE23 pKa = 5.03DD24 pKa = 4.02TQLLINAIDD33 pKa = 3.61AVLDD37 pKa = 3.84MEE39 pKa = 5.11HH40 pKa = 6.06VASVRR45 pKa = 11.84WEE47 pKa = 3.86QYY49 pKa = 9.52TPSFNDD55 pKa = 3.78GEE57 pKa = 4.22PCRR60 pKa = 11.84FSVHH64 pKa = 5.56RR65 pKa = 11.84VSVSLNGFSVPEE77 pKa = 4.33DD78 pKa = 3.96GYY80 pKa = 11.43EE81 pKa = 3.95SSEE84 pKa = 3.96DD85 pKa = 2.81RR86 pKa = 11.84WYY88 pKa = 10.54DD89 pKa = 3.16EE90 pKa = 4.45DD91 pKa = 4.31EE92 pKa = 4.03EE93 pKa = 5.21VFFDD97 pKa = 3.92EE98 pKa = 4.93YY99 pKa = 11.27EE100 pKa = 4.09LYY102 pKa = 10.46EE103 pKa = 4.3YY104 pKa = 8.71GTNEE108 pKa = 4.12DD109 pKa = 4.34GSRR112 pKa = 11.84NWDD115 pKa = 3.14EE116 pKa = 5.32CIYY119 pKa = 9.64EE120 pKa = 4.16VQGIPTEE127 pKa = 4.27EE128 pKa = 3.91IKK130 pKa = 11.08NALKK134 pKa = 9.61TLNQVLDD141 pKa = 3.98SGAHH145 pKa = 4.94YY146 pKa = 10.21AWLMSSFGDD155 pKa = 3.76PAEE158 pKa = 4.3VTATSDD164 pKa = 3.14TFDD167 pKa = 3.48VEE169 pKa = 4.54RR170 pKa = 11.84CEE172 pKa = 5.06HH173 pKa = 5.93EE174 pKa = 3.94

Molecular weight: 20.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9W1N0|A0A7G9W1N0_9CAUD Uncharacterized protein OS=Streptomyces phage MulchMansion OX=2768141 GN=129 PE=4 SV=1
MM1 pKa = 7.37ISKK4 pKa = 10.23EE5 pKa = 3.78EE6 pKa = 3.93RR7 pKa = 11.84QQLRR11 pKa = 11.84LARR14 pKa = 11.84SKK16 pKa = 11.44AEE18 pKa = 3.96LVRR21 pKa = 11.84TVAALFQMVMATATLCILVYY41 pKa = 10.78VNFMRR46 pKa = 5.65

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

214

0

214

34848

34

2105

162.8

18.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.219 ± 0.38

1.024 ± 0.115

6.546 ± 0.206

7.036 ± 0.334

3.848 ± 0.134

7.65 ± 0.215

1.92 ± 0.133

5.349 ± 0.145

6.379 ± 0.309

7.145 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.861 ± 0.127

4.454 ± 0.157

3.71 ± 0.154

3.159 ± 0.183

5.567 ± 0.197

6.043 ± 0.228

6.195 ± 0.398

7.24 ± 0.264

1.882 ± 0.092

3.771 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski