Amino acid dipepetide frequency for Streptomyces phage MulchMansion

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.346AlaAla: 7.346 ± 0.749
0.918AlaCys: 0.918 ± 0.212
4.993AlaAsp: 4.993 ± 0.367
5.51AlaGlu: 5.51 ± 0.46
2.841AlaPhe: 2.841 ± 0.302
5.51AlaGly: 5.51 ± 0.559
1.55AlaHis: 1.55 ± 0.228
4.965AlaIle: 4.965 ± 0.543
5.252AlaLys: 5.252 ± 0.447
6.916AlaLeu: 6.916 ± 0.592
2.468AlaMet: 2.468 ± 0.321
2.898AlaAsn: 2.898 ± 0.424
2.841AlaPro: 2.841 ± 0.445
2.927AlaGln: 2.927 ± 0.369
4.792AlaArg: 4.792 ± 0.4
4.62AlaSer: 4.62 ± 0.486
5.223AlaThr: 5.223 ± 0.715
5.653AlaVal: 5.653 ± 0.476
1.521AlaTrp: 1.521 ± 0.225
3.444AlaTyr: 3.444 ± 0.329
0.0AlaXaa: 0.0 ± 0.0
Cys
0.43CysAla: 0.43 ± 0.12
0.172CysCys: 0.172 ± 0.091
0.746CysAsp: 0.746 ± 0.189
0.517CysGlu: 0.517 ± 0.136
0.344CysPhe: 0.344 ± 0.099
1.148CysGly: 1.148 ± 0.21
0.23CysHis: 0.23 ± 0.076
0.603CysIle: 0.603 ± 0.146
0.775CysLys: 0.775 ± 0.157
0.66CysLeu: 0.66 ± 0.149
0.287CysMet: 0.287 ± 0.095
0.804CysAsn: 0.804 ± 0.161
0.574CysPro: 0.574 ± 0.143
0.201CysGln: 0.201 ± 0.082
0.746CysArg: 0.746 ± 0.164
0.517CysSer: 0.517 ± 0.153
0.517CysThr: 0.517 ± 0.125
0.488CysVal: 0.488 ± 0.118
0.143CysTrp: 0.143 ± 0.068
0.344CysTyr: 0.344 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
5.596AspAla: 5.596 ± 0.446
0.603AspCys: 0.603 ± 0.138
4.018AspAsp: 4.018 ± 0.443
5.223AspGlu: 5.223 ± 0.449
3.099AspPhe: 3.099 ± 0.3
5.395AspGly: 5.395 ± 0.501
1.004AspHis: 1.004 ± 0.196
3.731AspIle: 3.731 ± 0.409
3.96AspLys: 3.96 ± 0.41
4.764AspLeu: 4.764 ± 0.39
2.009AspMet: 2.009 ± 0.255
3.128AspAsn: 3.128 ± 0.275
2.238AspPro: 2.238 ± 0.261
1.55AspGln: 1.55 ± 0.186
2.755AspArg: 2.755 ± 0.27
3.989AspSer: 3.989 ± 0.371
3.386AspThr: 3.386 ± 0.448
4.591AspVal: 4.591 ± 0.483
1.664AspTrp: 1.664 ± 0.259
2.755AspTyr: 2.755 ± 0.366
0.0AspXaa: 0.0 ± 0.0
Glu
6.17GluAla: 6.17 ± 0.511
0.574GluCys: 0.574 ± 0.152
4.85GluAsp: 4.85 ± 0.376
4.649GluGlu: 4.649 ± 0.584
2.956GluPhe: 2.956 ± 0.304
4.505GluGly: 4.505 ± 0.412
1.779GluHis: 1.779 ± 0.301
4.276GluIle: 4.276 ± 0.399
4.534GluLys: 4.534 ± 0.449
5.309GluLeu: 5.309 ± 0.415
2.21GluMet: 2.21 ± 0.229
2.956GluAsn: 2.956 ± 0.323
2.411GluPro: 2.411 ± 0.365
2.669GluGln: 2.669 ± 0.348
3.989GluArg: 3.989 ± 0.358
3.415GluSer: 3.415 ± 0.38
3.731GluThr: 3.731 ± 0.418
5.108GluVal: 5.108 ± 0.429
1.492GluTrp: 1.492 ± 0.232
2.784GluTyr: 2.784 ± 0.376
0.0GluXaa: 0.0 ± 0.0
Phe
2.525PheAla: 2.525 ± 0.296
0.373PheCys: 0.373 ± 0.122
3.386PheAsp: 3.386 ± 0.296
3.472PheGlu: 3.472 ± 0.287
1.55PhePhe: 1.55 ± 0.228
3.243PheGly: 3.243 ± 0.285
0.717PheHis: 0.717 ± 0.18
1.894PheIle: 1.894 ± 0.189
2.095PheLys: 2.095 ± 0.274
1.951PheLeu: 1.951 ± 0.255
1.263PheMet: 1.263 ± 0.177
2.037PheAsn: 2.037 ± 0.227
1.09PhePro: 1.09 ± 0.168
1.148PheGln: 1.148 ± 0.213
1.951PheArg: 1.951 ± 0.216
2.583PheSer: 2.583 ± 0.295
2.554PheThr: 2.554 ± 0.291
2.611PheVal: 2.611 ± 0.3
0.689PheTrp: 0.689 ± 0.165
1.349PheTyr: 1.349 ± 0.168
0.0PheXaa: 0.0 ± 0.0
Gly
5.079GlyAla: 5.079 ± 0.448
0.545GlyCys: 0.545 ± 0.136
4.85GlyAsp: 4.85 ± 0.38
4.218GlyGlu: 4.218 ± 0.385
3.185GlyPhe: 3.185 ± 0.294
5.452GlyGly: 5.452 ± 0.516
1.722GlyHis: 1.722 ± 0.236
4.419GlyIle: 4.419 ± 0.418
5.252GlyLys: 5.252 ± 0.45
5.653GlyLeu: 5.653 ± 0.491
2.755GlyMet: 2.755 ± 0.263
3.501GlyAsn: 3.501 ± 0.339
2.238GlyPro: 2.238 ± 0.33
2.87GlyGln: 2.87 ± 0.345
4.018GlyArg: 4.018 ± 0.325
4.649GlySer: 4.649 ± 0.544
5.481GlyThr: 5.481 ± 0.658
5.998GlyVal: 5.998 ± 0.446
1.406GlyTrp: 1.406 ± 0.213
3.214GlyTyr: 3.214 ± 0.277
0.0GlyXaa: 0.0 ± 0.0
His
1.177HisAla: 1.177 ± 0.201
0.344HisCys: 0.344 ± 0.1
1.033HisAsp: 1.033 ± 0.184
1.119HisGlu: 1.119 ± 0.204
0.804HisPhe: 0.804 ± 0.173
1.664HisGly: 1.664 ± 0.246
0.488HisHis: 0.488 ± 0.125
1.148HisIle: 1.148 ± 0.186
1.09HisLys: 1.09 ± 0.188
1.406HisLeu: 1.406 ± 0.19
0.574HisMet: 0.574 ± 0.136
0.804HisAsn: 0.804 ± 0.155
0.861HisPro: 0.861 ± 0.203
0.574HisGln: 0.574 ± 0.128
1.521HisArg: 1.521 ± 0.248
1.148HisSer: 1.148 ± 0.178
0.918HisThr: 0.918 ± 0.187
1.291HisVal: 1.291 ± 0.208
0.373HisTrp: 0.373 ± 0.102
0.861HisTyr: 0.861 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
4.993IleAla: 4.993 ± 0.422
0.804IleCys: 0.804 ± 0.165
3.96IleAsp: 3.96 ± 0.461
4.448IleGlu: 4.448 ± 0.422
1.492IlePhe: 1.492 ± 0.209
3.874IleGly: 3.874 ± 0.355
0.89IleHis: 0.89 ± 0.164
2.181IleIle: 2.181 ± 0.267
3.673IleLys: 3.673 ± 0.362
3.587IleLeu: 3.587 ± 0.31
1.263IleMet: 1.263 ± 0.209
2.152IleAsn: 2.152 ± 0.271
2.066IlePro: 2.066 ± 0.249
1.751IleGln: 1.751 ± 0.27
3.472IleArg: 3.472 ± 0.28
3.185IleSer: 3.185 ± 0.256
3.185IleThr: 3.185 ± 0.333
4.132IleVal: 4.132 ± 0.376
0.746IleTrp: 0.746 ± 0.162
1.636IleTyr: 1.636 ± 0.205
0.0IleXaa: 0.0 ± 0.0
Lys
5.825LysAla: 5.825 ± 0.432
0.746LysCys: 0.746 ± 0.194
3.874LysAsp: 3.874 ± 0.392
4.161LysGlu: 4.161 ± 0.45
2.124LysPhe: 2.124 ± 0.251
4.391LysGly: 4.391 ± 0.393
1.291LysHis: 1.291 ± 0.253
3.243LysIle: 3.243 ± 0.346
4.792LysLys: 4.792 ± 0.507
4.218LysLeu: 4.218 ± 0.359
2.411LysMet: 2.411 ± 0.26
3.386LysAsn: 3.386 ± 0.318
2.525LysPro: 2.525 ± 0.382
1.98LysGln: 1.98 ± 0.216
3.759LysArg: 3.759 ± 0.444
3.3LysSer: 3.3 ± 0.33
3.673LysThr: 3.673 ± 0.293
4.477LysVal: 4.477 ± 0.325
1.09LysTrp: 1.09 ± 0.17
2.525LysTyr: 2.525 ± 0.275
0.0LysXaa: 0.0 ± 0.0
Leu
6.744LeuAla: 6.744 ± 0.487
0.918LeuCys: 0.918 ± 0.201
4.591LeuAsp: 4.591 ± 0.371
5.309LeuGlu: 5.309 ± 0.341
2.554LeuPhe: 2.554 ± 0.299
4.821LeuGly: 4.821 ± 0.433
1.263LeuHis: 1.263 ± 0.173
4.19LeuIle: 4.19 ± 0.338
4.477LeuLys: 4.477 ± 0.394
4.563LeuLeu: 4.563 ± 0.473
1.865LeuMet: 1.865 ± 0.239
3.271LeuAsn: 3.271 ± 0.364
2.784LeuPro: 2.784 ± 0.263
1.578LeuGln: 1.578 ± 0.232
4.104LeuArg: 4.104 ± 0.311
4.936LeuSer: 4.936 ± 0.329
5.051LeuThr: 5.051 ± 0.367
4.85LeuVal: 4.85 ± 0.504
1.205LeuTrp: 1.205 ± 0.182
2.382LeuTyr: 2.382 ± 0.31
0.0LeuXaa: 0.0 ± 0.0
Met
2.87MetAla: 2.87 ± 0.274
0.23MetCys: 0.23 ± 0.081
1.464MetAsp: 1.464 ± 0.183
1.722MetGlu: 1.722 ± 0.253
0.689MetPhe: 0.689 ± 0.164
2.066MetGly: 2.066 ± 0.298
0.574MetHis: 0.574 ± 0.131
1.291MetIle: 1.291 ± 0.203
1.837MetLys: 1.837 ± 0.255
2.009MetLeu: 2.009 ± 0.202
0.43MetMet: 0.43 ± 0.115
1.377MetAsn: 1.377 ± 0.222
1.177MetPro: 1.177 ± 0.205
1.033MetGln: 1.033 ± 0.238
2.095MetArg: 2.095 ± 0.258
2.353MetSer: 2.353 ± 0.244
2.296MetThr: 2.296 ± 0.266
1.751MetVal: 1.751 ± 0.275
0.517MetTrp: 0.517 ± 0.106
0.832MetTyr: 0.832 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
3.874AsnAla: 3.874 ± 0.342
0.287AsnCys: 0.287 ± 0.097
2.64AsnAsp: 2.64 ± 0.292
3.157AsnGlu: 3.157 ± 0.319
1.808AsnPhe: 1.808 ± 0.189
4.075AsnGly: 4.075 ± 0.389
1.234AsnHis: 1.234 ± 0.178
2.124AsnIle: 2.124 ± 0.268
2.812AsnLys: 2.812 ± 0.308
3.329AsnLeu: 3.329 ± 0.343
1.148AsnMet: 1.148 ± 0.177
2.267AsnAsn: 2.267 ± 0.313
2.066AsnPro: 2.066 ± 0.239
1.291AsnGln: 1.291 ± 0.243
2.21AsnArg: 2.21 ± 0.25
2.009AsnSer: 2.009 ± 0.291
2.87AsnThr: 2.87 ± 0.367
3.099AsnVal: 3.099 ± 0.363
0.775AsnTrp: 0.775 ± 0.155
1.464AsnTyr: 1.464 ± 0.202
0.0AsnXaa: 0.0 ± 0.0
Pro
2.87ProAla: 2.87 ± 0.301
0.344ProCys: 0.344 ± 0.121
3.013ProAsp: 3.013 ± 0.331
3.013ProGlu: 3.013 ± 0.337
1.578ProPhe: 1.578 ± 0.188
3.358ProGly: 3.358 ± 0.303
0.689ProHis: 0.689 ± 0.121
1.578ProIle: 1.578 ± 0.2
2.066ProLys: 2.066 ± 0.232
2.124ProLeu: 2.124 ± 0.23
0.832ProMet: 0.832 ± 0.171
1.664ProAsn: 1.664 ± 0.226
1.177ProPro: 1.177 ± 0.275
0.861ProGln: 0.861 ± 0.152
2.009ProArg: 2.009 ± 0.263
2.353ProSer: 2.353 ± 0.374
2.525ProThr: 2.525 ± 0.368
3.501ProVal: 3.501 ± 0.363
0.43ProTrp: 0.43 ± 0.111
1.119ProTyr: 1.119 ± 0.214
0.0ProXaa: 0.0 ± 0.0
Gln
2.927GlnAla: 2.927 ± 0.481
0.344GlnCys: 0.344 ± 0.11
1.291GlnAsp: 1.291 ± 0.201
2.554GlnGlu: 2.554 ± 0.33
1.664GlnPhe: 1.664 ± 0.235
1.865GlnGly: 1.865 ± 0.205
0.43GlnHis: 0.43 ± 0.112
1.492GlnIle: 1.492 ± 0.221
2.181GlnLys: 2.181 ± 0.342
2.497GlnLeu: 2.497 ± 0.219
1.09GlnMet: 1.09 ± 0.223
1.234GlnAsn: 1.234 ± 0.202
0.976GlnPro: 0.976 ± 0.171
0.89GlnGln: 0.89 ± 0.266
2.181GlnArg: 2.181 ± 0.367
1.808GlnSer: 1.808 ± 0.206
2.124GlnThr: 2.124 ± 0.264
2.411GlnVal: 2.411 ± 0.26
0.517GlnTrp: 0.517 ± 0.121
1.119GlnTyr: 1.119 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
4.477ArgAla: 4.477 ± 0.457
0.574ArgCys: 0.574 ± 0.156
3.415ArgAsp: 3.415 ± 0.356
3.759ArgGlu: 3.759 ± 0.372
2.497ArgPhe: 2.497 ± 0.295
4.19ArgGly: 4.19 ± 0.317
1.177ArgHis: 1.177 ± 0.212
2.984ArgIle: 2.984 ± 0.284
3.702ArgLys: 3.702 ± 0.399
3.931ArgLeu: 3.931 ± 0.353
1.751ArgMet: 1.751 ± 0.258
2.87ArgAsn: 2.87 ± 0.242
1.923ArgPro: 1.923 ± 0.246
1.894ArgGln: 1.894 ± 0.256
3.271ArgArg: 3.271 ± 0.443
3.185ArgSer: 3.185 ± 0.283
2.87ArgThr: 2.87 ± 0.322
3.472ArgVal: 3.472 ± 0.334
1.148ArgTrp: 1.148 ± 0.221
2.554ArgTyr: 2.554 ± 0.311
0.0ArgXaa: 0.0 ± 0.0
Ser
4.534SerAla: 4.534 ± 0.537
0.574SerCys: 0.574 ± 0.147
4.305SerAsp: 4.305 ± 0.386
3.931SerGlu: 3.931 ± 0.356
2.238SerPhe: 2.238 ± 0.252
5.969SerGly: 5.969 ± 0.542
0.976SerHis: 0.976 ± 0.197
3.415SerIle: 3.415 ± 0.303
3.874SerLys: 3.874 ± 0.416
4.907SerLeu: 4.907 ± 0.36
1.837SerMet: 1.837 ± 0.271
2.382SerAsn: 2.382 ± 0.337
2.124SerPro: 2.124 ± 0.289
1.808SerGln: 1.808 ± 0.23
2.841SerArg: 2.841 ± 0.244
4.018SerSer: 4.018 ± 0.516
3.185SerThr: 3.185 ± 0.624
4.591SerVal: 4.591 ± 0.413
1.119SerTrp: 1.119 ± 0.176
1.808SerTyr: 1.808 ± 0.267
0.0SerXaa: 0.0 ± 0.0
Thr
4.878ThrAla: 4.878 ± 0.545
0.717ThrCys: 0.717 ± 0.143
3.903ThrAsp: 3.903 ± 0.339
3.616ThrGlu: 3.616 ± 0.354
2.324ThrPhe: 2.324 ± 0.284
5.797ThrGly: 5.797 ± 0.83
0.947ThrHis: 0.947 ± 0.164
3.501ThrIle: 3.501 ± 0.364
3.386ThrLys: 3.386 ± 0.326
4.563ThrLeu: 4.563 ± 0.378
1.033ThrMet: 1.033 ± 0.202
2.755ThrAsn: 2.755 ± 0.447
3.128ThrPro: 3.128 ± 0.41
2.037ThrGln: 2.037 ± 0.313
2.984ThrArg: 2.984 ± 0.299
3.243ThrSer: 3.243 ± 0.298
4.218ThrThr: 4.218 ± 0.741
5.309ThrVal: 5.309 ± 0.537
1.464ThrTrp: 1.464 ± 0.164
2.152ThrTyr: 2.152 ± 0.237
0.0ThrXaa: 0.0 ± 0.0
Val
5.567ValAla: 5.567 ± 0.391
0.717ValCys: 0.717 ± 0.154
4.821ValAsp: 4.821 ± 0.5
5.596ValGlu: 5.596 ± 0.457
2.812ValPhe: 2.812 ± 0.311
4.591ValGly: 4.591 ± 0.361
1.263ValHis: 1.263 ± 0.197
3.989ValIle: 3.989 ± 0.368
4.735ValLys: 4.735 ± 0.378
4.563ValLeu: 4.563 ± 0.388
1.894ValMet: 1.894 ± 0.229
2.382ValAsn: 2.382 ± 0.333
3.013ValPro: 3.013 ± 0.339
2.497ValGln: 2.497 ± 0.267
4.333ValArg: 4.333 ± 0.335
5.223ValSer: 5.223 ± 0.417
4.477ValThr: 4.477 ± 0.55
5.366ValVal: 5.366 ± 0.442
1.406ValTrp: 1.406 ± 0.208
3.157ValTyr: 3.157 ± 0.36
0.0ValXaa: 0.0 ± 0.0
Trp
1.492TrpAla: 1.492 ± 0.213
0.201TrpCys: 0.201 ± 0.097
1.205TrpAsp: 1.205 ± 0.182
1.636TrpGlu: 1.636 ± 0.201
0.66TrpPhe: 0.66 ± 0.142
1.377TrpGly: 1.377 ± 0.193
0.459TrpHis: 0.459 ± 0.132
0.832TrpIle: 0.832 ± 0.142
1.32TrpLys: 1.32 ± 0.213
1.521TrpLeu: 1.521 ± 0.215
0.603TrpMet: 0.603 ± 0.143
0.976TrpAsn: 0.976 ± 0.182
0.517TrpPro: 0.517 ± 0.137
0.717TrpGln: 0.717 ± 0.154
0.66TrpArg: 0.66 ± 0.13
1.205TrpSer: 1.205 ± 0.196
1.234TrpThr: 1.234 ± 0.243
0.89TrpVal: 0.89 ± 0.178
0.459TrpTrp: 0.459 ± 0.125
0.861TrpTyr: 0.861 ± 0.152
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.812TyrAla: 2.812 ± 0.294
0.258TyrCys: 0.258 ± 0.087
3.099TyrAsp: 3.099 ± 0.394
2.755TyrGlu: 2.755 ± 0.331
1.263TyrPhe: 1.263 ± 0.209
3.214TyrGly: 3.214 ± 0.293
0.517TyrHis: 0.517 ± 0.116
1.636TyrIle: 1.636 ± 0.203
1.98TyrLys: 1.98 ± 0.244
2.984TyrLeu: 2.984 ± 0.276
0.861TyrMet: 0.861 ± 0.177
1.693TyrAsn: 1.693 ± 0.22
1.377TyrPro: 1.377 ± 0.219
1.32TyrGln: 1.32 ± 0.185
1.98TyrArg: 1.98 ± 0.243
2.698TyrSer: 2.698 ± 0.292
2.439TyrThr: 2.439 ± 0.242
2.812TyrVal: 2.812 ± 0.389
0.66TyrTrp: 0.66 ± 0.154
1.349TyrTyr: 1.349 ± 0.204
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 214 proteins (34848 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski