Mycolicibacterium llatzerense
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5405 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D1LJF1|A0A0D1LJF1_9MYCO Amidohydrolase OS=Mycolicibacterium llatzerense OX=280871 GN=TL10_01940 PE=4 SV=1
MM1 pKa = 6.89 THH3 pKa = 6.9 NIHH6 pKa = 6.46 IRR8 pKa = 11.84 TVDD11 pKa = 3.05 SSIRR15 pKa = 11.84 YY16 pKa = 7.41 PQALSYY22 pKa = 11.14 GEE24 pKa = 4.28 KK25 pKa = 10.66 DD26 pKa = 3.17 QILWVQIGDD35 pKa = 4.2 DD36 pKa = 4.39 ADD38 pKa = 3.96 HH39 pKa = 7.25 SDD41 pKa = 4.04 TIYY44 pKa = 10.77 FSPSYY49 pKa = 7.67 WQQYY53 pKa = 9.22 SVDD56 pKa = 3.79 PHH58 pKa = 8.48 SEE60 pKa = 4.16 DD61 pKa = 4.85 PLDD64 pKa = 4.67 LDD66 pKa = 4.61 FDD68 pKa = 4.55 EE69 pKa = 7.01 DD70 pKa = 6.01 DD71 pKa = 4.01 EE72 pKa = 7.21 DD73 pKa = 4.58 EE74 pKa = 6.52 DD75 pKa = 5.42 EE76 pKa = 6.31 DD77 pKa = 5.81 DD78 pKa = 6.05 DD79 pKa = 4.62 EE80 pKa = 4.74
Molecular weight: 9.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.592
IPC_protein 3.592
Toseland 3.363
ProMoST 3.757
Dawson 3.605
Bjellqvist 3.808
Wikipedia 3.579
Rodwell 3.427
Grimsley 3.274
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.999
Thurlkill 3.439
EMBOSS 3.579
Sillero 3.719
Patrickios 0.896
IPC_peptide 3.579
IPC2_peptide 3.694
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|A0A0D1LS20|A0A0D1LS20_9MYCO MarR family transcriptional regulator OS=Mycolicibacterium llatzerense OX=280871 GN=TL10_00130 PE=4 SV=1
MM1 pKa = 7.39 TLPSRR6 pKa = 11.84 TPWGTGLTVSAFVAAVTAAAIIVLSLGLLRR36 pKa = 11.84 VHH38 pKa = 7.14 PLLAVGLNAVAVGGLAPTVWSWRR61 pKa = 11.84 VRR63 pKa = 11.84 PVWRR67 pKa = 11.84 WFVWGAGLGVALGWVATLAIALSSS91 pKa = 3.46
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5405
0
5405
1698364
26
10330
314.2
33.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.947 ± 0.05
0.816 ± 0.009
6.277 ± 0.029
5.1 ± 0.036
3.115 ± 0.018
8.821 ± 0.038
2.264 ± 0.016
4.389 ± 0.026
2.398 ± 0.027
9.837 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.108 ± 0.015
2.357 ± 0.02
5.678 ± 0.031
3.131 ± 0.019
6.852 ± 0.038
5.395 ± 0.022
6.153 ± 0.026
8.651 ± 0.027
1.52 ± 0.011
2.193 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here