Vibrio furnissii
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4417 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q2V556|A0A0Q2V556_VIBFU Multidrug DMT transporter OS=Vibrio furnissii OX=29494 GN=AMR76_01215 PE=4 SV=1
MM1 pKa = 6.74 KK2 pKa = 10.06 TKK4 pKa = 10.15 IALFITAALASGSALANNDD23 pKa = 2.67 ATANLYY29 pKa = 7.77 LTNDD33 pKa = 3.4 SAVTITQVSSSQGNSALIDD52 pKa = 3.56 SSFSSGNTLSVYY64 pKa = 10.83 QNGGDD69 pKa = 3.35 NAATIRR75 pKa = 11.84 AFDD78 pKa = 3.62 VDD80 pKa = 3.92 GSTLAINQTGDD91 pKa = 3.29 TNTGSITALGAKK103 pKa = 9.1 NANFSINQGGDD114 pKa = 3.33 LNDD117 pKa = 4.07 ASIKK121 pKa = 8.35 TDD123 pKa = 2.81 NYY125 pKa = 10.1 HH126 pKa = 6.96 RR127 pKa = 11.84 GRR129 pKa = 11.84 AYY131 pKa = 10.67 SYY133 pKa = 11.72 DD134 pKa = 3.67 NDD136 pKa = 4.17 TVSVVQNGTEE146 pKa = 3.89 NTTSVEE152 pKa = 4.31 LKK154 pKa = 10.65 RR155 pKa = 11.84 GTDD158 pKa = 3.57 DD159 pKa = 4.75 SNVSLAASGSGNQANVVLDD178 pKa = 3.7 WADD181 pKa = 3.1 SSMVTGNVQGTDD193 pKa = 2.98 NVADD197 pKa = 3.68 VLIDD201 pKa = 3.35 SGYY204 pKa = 8.84 GNEE207 pKa = 4.64 VAFTQNGTANQLDD220 pKa = 3.96 VDD222 pKa = 4.12 VLYY225 pKa = 11.49 SNINTVHH232 pKa = 5.56 VNQTGSNNAADD243 pKa = 4.0 VDD245 pKa = 5.69 LIASDD250 pKa = 5.04 DD251 pKa = 3.56 NDD253 pKa = 3.48 IYY255 pKa = 10.66 VTQSNSNADD264 pKa = 3.3 ANVYY268 pKa = 9.24 VNWSSNNTISVMQTYY283 pKa = 11.14 GDD285 pKa = 3.72 TANVNLALSHH295 pKa = 6.46 NNTVTISQYY304 pKa = 11.33
Molecular weight: 31.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.669
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.478
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.745
Rodwell 3.541
Grimsley 3.376
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.202
Thurlkill 3.554
EMBOSS 3.745
Sillero 3.846
Patrickios 1.1
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.756
Protein with the highest isoelectric point:
>tr|A0A0Q2SHP7|A0A0Q2SHP7_VIBFU Uncharacterized protein OS=Vibrio furnissii OX=29494 GN=AMR76_05735 PE=4 SV=1
MM1 pKa = 7.06 IRR3 pKa = 11.84 HH4 pKa = 5.83 RR5 pKa = 11.84 PFHH8 pKa = 7.86 RR9 pKa = 11.84 MMLRR13 pKa = 11.84 ASILSWILVTLMPVINAHH31 pKa = 6.17 GSHH34 pKa = 6.42 AGVWATLCTINGFEE48 pKa = 4.58 LVKK51 pKa = 10.74 VDD53 pKa = 4.34 DD54 pKa = 5.26 GKK56 pKa = 10.11 PQTQHH61 pKa = 6.31 GKK63 pKa = 8.3 PCPFAHH69 pKa = 6.96 FSNFHH74 pKa = 5.63 TDD76 pKa = 2.87 KK77 pKa = 11.34 LPTTRR82 pKa = 11.84 PNTTQSAVISDD93 pKa = 4.56 RR94 pKa = 11.84 YY95 pKa = 8.6 TFLALSVRR103 pKa = 11.84 FEE105 pKa = 4.18 RR106 pKa = 11.84 QVPRR110 pKa = 11.84 GPPQSATSS118 pKa = 3.42
Molecular weight: 13.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.502
IPC_protein 10.175
Toseland 10.73
ProMoST 10.379
Dawson 10.789
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.994
Grimsley 10.818
Solomon 10.935
Lehninger 10.921
Nozaki 10.73
DTASelect 10.482
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.73
Patrickios 10.804
IPC_peptide 10.95
IPC2_peptide 9.677
IPC2.peptide.svr19 8.644
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4417
0
4417
1435389
36
1652
325.0
36.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.374 ± 0.04
1.04 ± 0.014
5.499 ± 0.034
5.901 ± 0.035
4.041 ± 0.027
6.8 ± 0.034
2.526 ± 0.021
5.971 ± 0.027
4.662 ± 0.032
10.625 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.785 ± 0.02
3.872 ± 0.024
4.006 ± 0.023
4.871 ± 0.032
4.88 ± 0.029
6.28 ± 0.028
5.372 ± 0.021
7.239 ± 0.033
1.285 ± 0.015
2.971 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here