Marivita hallyeonensis
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4131 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5RDH5|A0A1M5RDH5_9RHOB 5-aminolevulinate synthase OS=Marivita hallyeonensis OX=996342 GN=SAMN05443551_1699 PE=3 SV=1
MM1 pKa = 7.59 NDD3 pKa = 3.41 ASPHH7 pKa = 5.78 IEE9 pKa = 4.24 GAPLIAPSSTDD20 pKa = 2.93 HH21 pKa = 6.59 EE22 pKa = 5.42 LYY24 pKa = 10.78 DD25 pKa = 4.04 QVVEE29 pKa = 4.41 ACRR32 pKa = 11.84 SVYY35 pKa = 10.64 DD36 pKa = 3.59 PEE38 pKa = 5.2 IPVNIYY44 pKa = 10.88 DD45 pKa = 4.22 LGLVYY50 pKa = 10.37 TIDD53 pKa = 3.25 INAEE57 pKa = 3.77 NEE59 pKa = 3.79 VRR61 pKa = 11.84 VIMTLTAPGCPVAGEE76 pKa = 4.1 MPGWVAEE83 pKa = 4.75 AIEE86 pKa = 4.23 PLPGVKK92 pKa = 9.96 HH93 pKa = 6.14 VDD95 pKa = 3.16 VDD97 pKa = 4.47 LTWEE101 pKa = 4.65 PPWGMDD107 pKa = 3.35 MMSDD111 pKa = 3.68 EE112 pKa = 4.89 ARR114 pKa = 11.84 LEE116 pKa = 4.14 LGFMM120 pKa = 4.54
Molecular weight: 13.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.872
IPC_protein 3.808
Toseland 3.617
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.935
Patrickios 0.896
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|A0A1M5MJN7|A0A1M5MJN7_9RHOB Hydrogenase small subunit OS=Marivita hallyeonensis OX=996342 GN=SAMN05443551_0546 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.45 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4131
0
4131
1271774
25
2158
307.9
33.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.773 ± 0.043
0.907 ± 0.013
6.398 ± 0.032
5.889 ± 0.034
3.895 ± 0.021
8.405 ± 0.037
2.081 ± 0.019
5.27 ± 0.023
3.238 ± 0.031
9.905 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.787 ± 0.019
2.692 ± 0.023
5.035 ± 0.027
3.196 ± 0.022
6.37 ± 0.037
5.301 ± 0.027
5.771 ± 0.026
7.443 ± 0.029
1.424 ± 0.018
2.219 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here