Tenacibaculum holothuriorum
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2802 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y2PD47|A0A1Y2PD47_9FLAO Uncharacterized protein (Fragment) OS=Tenacibaculum holothuriorum OX=1635173 GN=WH52_09165 PE=4 SV=1
MM1 pKa = 7.4 EE2 pKa = 4.33 VDD4 pKa = 3.31 INIKK8 pKa = 10.12 IKK10 pKa = 10.62 DD11 pKa = 3.45 RR12 pKa = 11.84 AGIIHH17 pKa = 6.6 EE18 pKa = 4.57 VVAPTDD24 pKa = 3.24 MAMNLMEE31 pKa = 4.24 VVKK34 pKa = 10.34 SYY36 pKa = 11.15 EE37 pKa = 3.94 LAPEE41 pKa = 4.21 GTIGICGGMAMCASCQCYY59 pKa = 9.39 VVSNHH64 pKa = 6.0 EE65 pKa = 4.42 LPEE68 pKa = 4.26 KK69 pKa = 10.31 TDD71 pKa = 4.78 DD72 pKa = 3.82 EE73 pKa = 4.71 DD74 pKa = 6.05 AMLAEE79 pKa = 4.55 AFDD82 pKa = 3.76 VQEE85 pKa = 4.1 NSRR88 pKa = 11.84 LGCQIQMTPEE98 pKa = 4.13 CEE100 pKa = 4.05 GLEE103 pKa = 4.11 VEE105 pKa = 5.32 LAPEE109 pKa = 4.07 SS110 pKa = 3.78
Molecular weight: 12.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.838
IPC2_protein 4.088
IPC_protein 3.986
Toseland 3.821
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.808
Rodwell 3.821
Grimsley 3.732
Solomon 3.923
Lehninger 3.872
Nozaki 4.05
DTASelect 4.164
Thurlkill 3.846
EMBOSS 3.821
Sillero 4.088
Patrickios 0.947
IPC_peptide 3.923
IPC2_peptide 4.075
IPC2.peptide.svr19 3.983
Protein with the highest isoelectric point:
>tr|A0A1Y2PDL6|A0A1Y2PDL6_9FLAO 3-demethylubiquinone-9 3-methyltransferase OS=Tenacibaculum holothuriorum OX=1635173 GN=WH52_05740 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 10.14 FIHH5 pKa = 5.76 SVRR8 pKa = 11.84 HH9 pKa = 4.19 FFEE12 pKa = 3.9 RR13 pKa = 11.84 HH14 pKa = 4.43 GFNVSSRR21 pKa = 11.84 FADD24 pKa = 3.65 RR25 pKa = 11.84 LGMRR29 pKa = 11.84 ATNVRR34 pKa = 11.84 LFFIYY39 pKa = 10.25 ISFVTVGLSFGLYY52 pKa = 8.31 LTLAFLLRR60 pKa = 11.84 LKK62 pKa = 11.19 DD63 pKa = 3.26 MVYY66 pKa = 10.16 TKK68 pKa = 9.81 RR69 pKa = 11.84 TSVFDD74 pKa = 3.66 LL75 pKa = 4.18
Molecular weight: 8.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.303
IPC2_protein 10.072
IPC_protein 11.067
Toseland 11.023
ProMoST 11.096
Dawson 11.096
Bjellqvist 10.935
Wikipedia 11.418
Rodwell 11.125
Grimsley 11.155
Solomon 11.359
Lehninger 11.301
Nozaki 10.994
DTASelect 10.935
Thurlkill 11.023
EMBOSS 11.462
Sillero 11.052
Patrickios 10.906
IPC_peptide 11.359
IPC2_peptide 10.131
IPC2.peptide.svr19 8.339
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2802
0
2802
936333
48
2398
334.2
37.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.995 ± 0.051
0.683 ± 0.015
5.212 ± 0.036
6.697 ± 0.048
5.344 ± 0.041
6.153 ± 0.05
1.725 ± 0.017
8.064 ± 0.042
8.776 ± 0.067
9.211 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.028 ± 0.025
6.652 ± 0.058
3.165 ± 0.025
3.38 ± 0.025
3.284 ± 0.03
6.382 ± 0.04
5.916 ± 0.052
6.295 ± 0.035
0.992 ± 0.014
4.047 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here