Actinoalloteichus hoggarensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinoalloteichus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5520 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221VX27|A0A221VX27_9PSEU UDP-glucose 4-epimerase OS=Actinoalloteichus hoggarensis OX=1470176 GN=AHOG_02225 PE=4 SV=1
MM1 pKa = 8.13RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84TLNAMSVMGLASALALTACGSGGGDD29 pKa = 3.29GDD31 pKa = 3.99GPAAVTFWDD40 pKa = 3.62TSGPAEE46 pKa = 4.11SPVFARR52 pKa = 11.84LAQDD56 pKa = 3.36CGEE59 pKa = 4.26AGGYY63 pKa = 8.82QVDD66 pKa = 3.81IEE68 pKa = 4.41QVAFDD73 pKa = 3.77QALNNYY79 pKa = 9.01RR80 pKa = 11.84NAAQGGQGPDD90 pKa = 2.92VFRR93 pKa = 11.84AEE95 pKa = 4.49VAWVPQLAEE104 pKa = 4.01NGLIQDD110 pKa = 4.69LSEE113 pKa = 4.36TDD115 pKa = 3.68LADD118 pKa = 3.84PADD121 pKa = 4.04FLEE124 pKa = 4.99TPWSSTQYY132 pKa = 10.87DD133 pKa = 3.3GRR135 pKa = 11.84TYY137 pKa = 10.92GVPQVTDD144 pKa = 3.57TLALLYY150 pKa = 10.93NRR152 pKa = 11.84DD153 pKa = 3.93LLDD156 pKa = 3.82EE157 pKa = 5.29AGVEE161 pKa = 4.56PPTSWAEE168 pKa = 4.07LAEE171 pKa = 4.31SAPPLGGEE179 pKa = 4.42SAIFLNNDD187 pKa = 2.72AYY189 pKa = 10.76YY190 pKa = 11.01ALPFVYY196 pKa = 9.92GAGGDD201 pKa = 3.93LVDD204 pKa = 4.99TEE206 pKa = 4.24AQEE209 pKa = 4.17ILVNSPEE216 pKa = 4.05AVEE219 pKa = 4.62GLTTAKK225 pKa = 10.7DD226 pKa = 3.76LLDD229 pKa = 3.95EE230 pKa = 4.91GAAQTALDD238 pKa = 3.69QGNSYY243 pKa = 11.63GNMKK247 pKa = 10.29AAFVAGDD254 pKa = 3.29VAMIIDD260 pKa = 4.54GPWAVAEE267 pKa = 4.38LLEE270 pKa = 5.45GDD272 pKa = 4.47AFADD276 pKa = 3.91PEE278 pKa = 4.32NLGIAPVPGPEE289 pKa = 4.37DD290 pKa = 3.4GAGNSPVGGHH300 pKa = 7.16DD301 pKa = 3.34YY302 pKa = 11.11VIRR305 pKa = 11.84QGSAATEE312 pKa = 4.1SAQDD316 pKa = 4.7LIACLSSTEE325 pKa = 4.07SQITVATEE333 pKa = 3.99LGLLPTRR340 pKa = 11.84EE341 pKa = 3.88SAYY344 pKa = 10.66ADD346 pKa = 3.64PAVAAQPIVSAFSPVIEE363 pKa = 4.42RR364 pKa = 11.84AHH366 pKa = 5.63PRR368 pKa = 11.84PWIPEE373 pKa = 3.93GGEE376 pKa = 3.84LFDD379 pKa = 4.83PLKK382 pKa = 10.48IAYY385 pKa = 9.95SDD387 pKa = 3.21ILAGNVEE394 pKa = 4.29PQDD397 pKa = 3.97ALDD400 pKa = 4.0EE401 pKa = 4.4VATIYY406 pKa = 10.73QDD408 pKa = 3.79TILTEE413 pKa = 4.27YY414 pKa = 10.71SVSS417 pKa = 3.38

Molecular weight: 43.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221VW50|A0A221VW50_9PSEU 3-oxoacyl-[acyl-carrier-protein] reductase FabG OS=Actinoalloteichus hoggarensis OX=1470176 GN=fabG1 PE=3 SV=1
MM1 pKa = 7.47SSRR4 pKa = 11.84RR5 pKa = 11.84SGTTTPPARR14 pKa = 11.84ALRR17 pKa = 11.84RR18 pKa = 11.84PRR20 pKa = 11.84TTSRR24 pKa = 11.84AGLRR28 pKa = 11.84RR29 pKa = 11.84VPTASPRR36 pKa = 11.84PVAGTLPAKK45 pKa = 10.39ARR47 pKa = 11.84TISVPAAQRR56 pKa = 11.84EE57 pKa = 4.45DD58 pKa = 2.99IRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84APGRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84ARR70 pKa = 11.84RR71 pKa = 11.84SRR73 pKa = 11.84RR74 pKa = 11.84ARR76 pKa = 11.84ARR78 pKa = 11.84PRR80 pKa = 11.84RR81 pKa = 11.84HH82 pKa = 6.27LGGTPRR88 pKa = 11.84PSLAVFPRR96 pKa = 11.84PSLAVFPRR104 pKa = 11.84PNLAGPPRR112 pKa = 11.84GRR114 pKa = 11.84RR115 pKa = 11.84AGLPPRR121 pKa = 11.84ARR123 pKa = 11.84RR124 pKa = 11.84RR125 pKa = 11.84PRR127 pKa = 11.84TRR129 pKa = 11.84SDD131 pKa = 2.55RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84AVLQAAVADD143 pKa = 4.29AGPIRR148 pKa = 11.84MNSSRR153 pKa = 11.84RR154 pKa = 11.84GARR157 pKa = 11.84AGTRR161 pKa = 11.84RR162 pKa = 11.84TPRR165 pKa = 11.84TARR168 pKa = 11.84RR169 pKa = 11.84PRR171 pKa = 11.84RR172 pKa = 11.84RR173 pKa = 11.84PSSPSPPSSRR183 pKa = 11.84GRR185 pKa = 11.84RR186 pKa = 11.84PGGWMPPIRR195 pKa = 11.84SRR197 pKa = 11.84GSGGVRR203 pKa = 11.84HH204 pKa = 6.83HH205 pKa = 6.64GRR207 pKa = 11.84PHH209 pKa = 6.5HH210 pKa = 5.71RR211 pKa = 11.84TAMAGSRR218 pKa = 11.84RR219 pKa = 11.84PVVPSTVPSTRR230 pKa = 11.84LPAGGPVRR238 pKa = 11.84SSPRR242 pKa = 11.84RR243 pKa = 11.84RR244 pKa = 11.84DD245 pKa = 2.88RR246 pKa = 11.84RR247 pKa = 11.84RR248 pKa = 11.84PLGRR252 pKa = 11.84VGTRR256 pKa = 11.84RR257 pKa = 11.84TVTNRR262 pKa = 11.84RR263 pKa = 11.84TRR265 pKa = 11.84IRR267 pKa = 11.84GRR269 pKa = 11.84ARR271 pKa = 11.84RR272 pKa = 11.84RR273 pKa = 3.23

Molecular weight: 30.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5520

0

5520

1798018

29

8880

325.7

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.527 ± 0.053

0.761 ± 0.01

6.358 ± 0.027

5.869 ± 0.03

2.66 ± 0.019

9.396 ± 0.036

2.201 ± 0.015

3.305 ± 0.024

1.236 ± 0.023

10.412 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.669 ± 0.012

1.554 ± 0.017

6.186 ± 0.036

2.501 ± 0.018

8.867 ± 0.042

5.443 ± 0.023

5.975 ± 0.021

8.881 ± 0.032

1.475 ± 0.013

1.726 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski