Bacillus phage Carmel_SA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wbetavirus; unclassified Wbetavirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A288WGB0|A0A288WGB0_9CAUD Uncharacterized protein OS=Bacillus phage Carmel_SA OX=1983578 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 9.33LTLRR6 pKa = 11.84INNEE10 pKa = 3.77EE11 pKa = 4.02KK12 pKa = 10.38TFNLPAFIPARR23 pKa = 11.84LIRR26 pKa = 11.84QAPEE30 pKa = 3.56LADD33 pKa = 3.2IPNNPEE39 pKa = 3.67AADD42 pKa = 3.96LDD44 pKa = 4.21KK45 pKa = 10.48MVQYY49 pKa = 10.47VVHH52 pKa = 6.61VYY54 pKa = 10.88GEE56 pKa = 4.19QFTVDD61 pKa = 3.81QYY63 pKa = 11.57WDD65 pKa = 3.51GVDD68 pKa = 3.15ARR70 pKa = 11.84KK71 pKa = 9.16FLLTTTNVINALVNEE86 pKa = 4.36TVEE89 pKa = 4.09AAGANPVADD98 pKa = 3.71GAEE101 pKa = 4.12NPNVV105 pKa = 3.33

Molecular weight: 11.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A288WG55|A0A288WG55_9CAUD Major tail protein OS=Bacillus phage Carmel_SA OX=1983578 PE=4 SV=1
MM1 pKa = 7.83CGNQNEE7 pKa = 4.74FIKK10 pKa = 10.72EE11 pKa = 3.83GSIKK15 pKa = 9.65MRR17 pKa = 11.84TWKK20 pKa = 10.2KK21 pKa = 9.78KK22 pKa = 8.18HH23 pKa = 5.82IKK25 pKa = 9.91RR26 pKa = 11.84AFLNRR31 pKa = 11.84QKK33 pKa = 10.83EE34 pKa = 4.15VDD36 pKa = 3.58KK37 pKa = 11.17EE38 pKa = 3.89RR39 pKa = 11.84TAAAWRR45 pKa = 11.84NIFVKK50 pKa = 10.61SGIIKK55 pKa = 10.38

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12313

50

1321

219.9

25.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.083 ± 0.584

0.926 ± 0.173

5.75 ± 0.202

8.56 ± 0.265

4.207 ± 0.228

5.685 ± 0.344

1.697 ± 0.153

6.741 ± 0.252

9.705 ± 0.327

8.601 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.193

5.401 ± 0.212

2.631 ± 0.234

4.15 ± 0.276

4.621 ± 0.274

5.709 ± 0.213

5.588 ± 0.314

6.383 ± 0.229

1.121 ± 0.144

3.809 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski