Amino acid dipepetide frequency for Bacillus phage Carmel_SA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.361AlaAla: 5.361 ± 1.304
0.325AlaCys: 0.325 ± 0.178
4.061AlaAsp: 4.061 ± 0.622
4.305AlaGlu: 4.305 ± 0.61
3.005AlaPhe: 3.005 ± 0.45
4.142AlaGly: 4.142 ± 0.871
0.812AlaHis: 0.812 ± 0.27
4.792AlaIle: 4.792 ± 0.492
4.548AlaLys: 4.548 ± 0.537
5.361AlaLeu: 5.361 ± 0.669
1.137AlaMet: 1.137 ± 0.355
3.574AlaAsn: 3.574 ± 0.582
1.381AlaPro: 1.381 ± 0.316
2.355AlaGln: 2.355 ± 0.547
2.518AlaArg: 2.518 ± 0.539
3.411AlaSer: 3.411 ± 0.638
3.249AlaThr: 3.249 ± 0.807
3.493AlaVal: 3.493 ± 0.844
0.893AlaTrp: 0.893 ± 0.242
2.112AlaTyr: 2.112 ± 0.546
0.0AlaXaa: 0.0 ± 0.0
Cys
0.162CysAla: 0.162 ± 0.128
0.162CysCys: 0.162 ± 0.107
0.406CysAsp: 0.406 ± 0.144
0.65CysGlu: 0.65 ± 0.32
0.569CysPhe: 0.569 ± 0.197
0.569CysGly: 0.569 ± 0.344
0.244CysHis: 0.244 ± 0.13
0.65CysIle: 0.65 ± 0.218
0.812CysLys: 0.812 ± 0.245
0.65CysLeu: 0.65 ± 0.201
0.325CysMet: 0.325 ± 0.147
0.487CysAsn: 0.487 ± 0.207
0.65CysPro: 0.65 ± 0.22
0.325CysGln: 0.325 ± 0.134
0.569CysArg: 0.569 ± 0.228
0.487CysSer: 0.487 ± 0.175
0.244CysThr: 0.244 ± 0.129
0.569CysVal: 0.569 ± 0.196
0.0CysTrp: 0.0 ± 0.0
0.731CysTyr: 0.731 ± 0.331
0.0CysXaa: 0.0 ± 0.0
Asp
3.168AspAla: 3.168 ± 0.548
0.325AspCys: 0.325 ± 0.15
3.411AspAsp: 3.411 ± 0.494
4.792AspGlu: 4.792 ± 0.734
2.843AspPhe: 2.843 ± 0.457
3.817AspGly: 3.817 ± 0.518
0.569AspHis: 0.569 ± 0.203
3.98AspIle: 3.98 ± 0.364
6.579AspLys: 6.579 ± 0.612
4.955AspLeu: 4.955 ± 0.618
1.949AspMet: 1.949 ± 0.315
2.762AspAsn: 2.762 ± 0.454
1.624AspPro: 1.624 ± 0.429
1.949AspGln: 1.949 ± 0.403
2.599AspArg: 2.599 ± 0.393
2.112AspSer: 2.112 ± 0.39
3.33AspThr: 3.33 ± 0.47
3.655AspVal: 3.655 ± 0.532
0.569AspTrp: 0.569 ± 0.18
2.518AspTyr: 2.518 ± 0.596
0.0AspXaa: 0.0 ± 0.0
Glu
5.523GluAla: 5.523 ± 0.492
1.137GluCys: 1.137 ± 0.438
4.711GluAsp: 4.711 ± 0.63
8.203GluGlu: 8.203 ± 1.082
3.086GluPhe: 3.086 ± 0.439
4.548GluGly: 4.548 ± 0.519
1.218GluHis: 1.218 ± 0.289
7.391GluIle: 7.391 ± 0.575
8.203GluLys: 8.203 ± 0.723
8.366GluLeu: 8.366 ± 0.829
3.005GluMet: 3.005 ± 0.503
3.736GluAsn: 3.736 ± 0.621
1.3GluPro: 1.3 ± 0.39
4.711GluGln: 4.711 ± 0.821
3.817GluArg: 3.817 ± 0.839
4.63GluSer: 4.63 ± 0.872
4.548GluThr: 4.548 ± 0.583
4.224GluVal: 4.224 ± 0.665
0.893GluTrp: 0.893 ± 0.225
2.355GluTyr: 2.355 ± 0.461
0.0GluXaa: 0.0 ± 0.0
Phe
2.843PheAla: 2.843 ± 0.479
0.487PheCys: 0.487 ± 0.203
2.68PheAsp: 2.68 ± 0.554
2.924PheGlu: 2.924 ± 0.447
1.706PhePhe: 1.706 ± 0.381
2.031PheGly: 2.031 ± 0.412
0.65PheHis: 0.65 ± 0.218
3.168PheIle: 3.168 ± 0.658
4.386PheLys: 4.386 ± 0.391
3.655PheLeu: 3.655 ± 0.518
1.381PheMet: 1.381 ± 0.293
2.518PheAsn: 2.518 ± 0.395
1.056PhePro: 1.056 ± 0.392
1.624PheGln: 1.624 ± 0.353
1.949PheArg: 1.949 ± 0.363
2.274PheSer: 2.274 ± 0.405
2.599PheThr: 2.599 ± 0.462
2.031PheVal: 2.031 ± 0.335
0.487PheTrp: 0.487 ± 0.189
1.624PheTyr: 1.624 ± 0.378
0.0PheXaa: 0.0 ± 0.0
Gly
3.411GlyAla: 3.411 ± 0.567
0.65GlyCys: 0.65 ± 0.227
3.249GlyAsp: 3.249 ± 0.447
5.279GlyGlu: 5.279 ± 0.536
3.33GlyPhe: 3.33 ± 0.439
3.086GlyGly: 3.086 ± 0.597
0.731GlyHis: 0.731 ± 0.213
4.711GlyIle: 4.711 ± 0.771
5.523GlyLys: 5.523 ± 0.625
5.604GlyLeu: 5.604 ± 0.835
1.868GlyMet: 1.868 ± 0.426
2.599GlyAsn: 2.599 ± 0.554
0.731GlyPro: 0.731 ± 0.209
1.543GlyGln: 1.543 ± 0.243
1.787GlyArg: 1.787 ± 0.422
2.599GlySer: 2.599 ± 0.429
3.086GlyThr: 3.086 ± 0.656
3.98GlyVal: 3.98 ± 0.625
0.812GlyTrp: 0.812 ± 0.303
2.274GlyTyr: 2.274 ± 0.412
0.0GlyXaa: 0.0 ± 0.0
His
0.893HisAla: 0.893 ± 0.271
0.65HisCys: 0.65 ± 0.241
0.569HisAsp: 0.569 ± 0.187
1.381HisGlu: 1.381 ± 0.279
0.812HisPhe: 0.812 ± 0.302
0.569HisGly: 0.569 ± 0.157
0.65HisHis: 0.65 ± 0.255
1.137HisIle: 1.137 ± 0.275
1.3HisLys: 1.3 ± 0.339
1.462HisLeu: 1.462 ± 0.328
0.487HisMet: 0.487 ± 0.167
0.731HisAsn: 0.731 ± 0.224
0.325HisPro: 0.325 ± 0.169
0.65HisGln: 0.65 ± 0.236
1.137HisArg: 1.137 ± 0.263
0.65HisSer: 0.65 ± 0.211
1.056HisThr: 1.056 ± 0.302
0.812HisVal: 0.812 ± 0.282
0.406HisTrp: 0.406 ± 0.177
1.3HisTyr: 1.3 ± 0.33
0.0HisXaa: 0.0 ± 0.0
Ile
3.98IleAla: 3.98 ± 0.542
0.569IleCys: 0.569 ± 0.188
4.711IleAsp: 4.711 ± 0.73
6.01IleGlu: 6.01 ± 0.536
1.868IlePhe: 1.868 ± 0.422
3.98IleGly: 3.98 ± 0.535
0.975IleHis: 0.975 ± 0.315
3.98IleIle: 3.98 ± 0.532
7.066IleLys: 7.066 ± 0.764
5.117IleLeu: 5.117 ± 0.603
1.624IleMet: 1.624 ± 0.427
3.899IleAsn: 3.899 ± 0.458
2.599IlePro: 2.599 ± 0.51
3.005IleGln: 3.005 ± 0.508
3.736IleArg: 3.736 ± 0.453
4.061IleSer: 4.061 ± 0.51
3.249IleThr: 3.249 ± 0.479
3.899IleVal: 3.899 ± 0.534
0.569IleTrp: 0.569 ± 0.221
2.518IleTyr: 2.518 ± 0.586
0.0IleXaa: 0.0 ± 0.0
Lys
4.711LysAla: 4.711 ± 0.881
0.893LysCys: 0.893 ± 0.248
4.061LysAsp: 4.061 ± 0.621
9.097LysGlu: 9.097 ± 1.051
3.411LysPhe: 3.411 ± 0.49
5.604LysGly: 5.604 ± 0.643
2.031LysHis: 2.031 ± 0.36
6.66LysIle: 6.66 ± 0.557
9.34LysLys: 9.34 ± 1.105
8.691LysLeu: 8.691 ± 0.878
3.33LysMet: 3.33 ± 0.464
5.117LysAsn: 5.117 ± 0.568
2.68LysPro: 2.68 ± 0.49
3.655LysGln: 3.655 ± 0.464
4.873LysArg: 4.873 ± 0.744
5.767LysSer: 5.767 ± 0.65
5.767LysThr: 5.767 ± 0.765
6.741LysVal: 6.741 ± 0.607
0.893LysTrp: 0.893 ± 0.271
3.736LysTyr: 3.736 ± 0.753
0.0LysXaa: 0.0 ± 0.0
Leu
5.198LeuAla: 5.198 ± 0.676
0.569LeuCys: 0.569 ± 0.216
5.198LeuAsp: 5.198 ± 0.582
7.716LeuGlu: 7.716 ± 0.811
3.655LeuPhe: 3.655 ± 0.603
4.792LeuGly: 4.792 ± 0.785
1.3LeuHis: 1.3 ± 0.245
4.63LeuIle: 4.63 ± 0.641
9.828LeuLys: 9.828 ± 0.826
6.01LeuLeu: 6.01 ± 0.811
1.868LeuMet: 1.868 ± 0.4
5.279LeuAsn: 5.279 ± 0.634
2.193LeuPro: 2.193 ± 0.422
4.224LeuGln: 4.224 ± 0.652
4.142LeuArg: 4.142 ± 0.702
5.036LeuSer: 5.036 ± 0.564
5.198LeuThr: 5.198 ± 0.562
5.036LeuVal: 5.036 ± 0.695
0.812LeuTrp: 0.812 ± 0.319
3.33LeuTyr: 3.33 ± 0.527
0.0LeuXaa: 0.0 ± 0.0
Met
1.462MetAla: 1.462 ± 0.329
0.162MetCys: 0.162 ± 0.122
1.543MetAsp: 1.543 ± 0.404
1.624MetGlu: 1.624 ± 0.344
1.056MetPhe: 1.056 ± 0.37
1.787MetGly: 1.787 ± 0.372
0.65MetHis: 0.65 ± 0.269
1.624MetIle: 1.624 ± 0.329
3.411MetLys: 3.411 ± 0.539
1.462MetLeu: 1.462 ± 0.301
0.487MetMet: 0.487 ± 0.197
1.543MetAsn: 1.543 ± 0.364
0.812MetPro: 0.812 ± 0.244
0.893MetGln: 0.893 ± 0.302
1.3MetArg: 1.3 ± 0.351
1.949MetSer: 1.949 ± 0.383
1.949MetThr: 1.949 ± 0.326
1.218MetVal: 1.218 ± 0.336
0.731MetTrp: 0.731 ± 0.222
0.65MetTyr: 0.65 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
3.086AsnAla: 3.086 ± 0.489
0.406AsnCys: 0.406 ± 0.179
2.355AsnAsp: 2.355 ± 0.414
5.117AsnGlu: 5.117 ± 0.679
1.949AsnPhe: 1.949 ± 0.468
3.493AsnGly: 3.493 ± 0.536
1.462AsnHis: 1.462 ± 0.399
3.33AsnIle: 3.33 ± 0.55
5.117AsnLys: 5.117 ± 0.731
3.736AsnLeu: 3.736 ± 0.44
1.462AsnMet: 1.462 ± 0.413
2.518AsnAsn: 2.518 ± 0.581
1.868AsnPro: 1.868 ± 0.388
2.924AsnGln: 2.924 ± 0.406
3.411AsnArg: 3.411 ± 0.603
3.168AsnSer: 3.168 ± 0.565
2.274AsnThr: 2.274 ± 0.371
4.061AsnVal: 4.061 ± 0.673
0.731AsnTrp: 0.731 ± 0.281
1.543AsnTyr: 1.543 ± 0.353
0.0AsnXaa: 0.0 ± 0.0
Pro
1.949ProAla: 1.949 ± 0.44
0.244ProCys: 0.244 ± 0.162
1.381ProAsp: 1.381 ± 0.345
2.599ProGlu: 2.599 ± 0.444
0.65ProPhe: 0.65 ± 0.205
0.65ProGly: 0.65 ± 0.188
0.731ProHis: 0.731 ± 0.266
1.624ProIle: 1.624 ± 0.398
1.706ProLys: 1.706 ± 0.379
2.437ProLeu: 2.437 ± 0.627
0.65ProMet: 0.65 ± 0.253
1.543ProAsn: 1.543 ± 0.357
0.569ProPro: 0.569 ± 0.211
0.731ProGln: 0.731 ± 0.239
1.056ProArg: 1.056 ± 0.318
2.193ProSer: 2.193 ± 0.339
1.706ProThr: 1.706 ± 0.33
2.355ProVal: 2.355 ± 0.412
0.162ProTrp: 0.162 ± 0.153
1.381ProTyr: 1.381 ± 0.361
0.0ProXaa: 0.0 ± 0.0
Gln
2.762GlnAla: 2.762 ± 0.752
0.162GlnCys: 0.162 ± 0.138
1.787GlnAsp: 1.787 ± 0.432
3.574GlnGlu: 3.574 ± 0.555
1.868GlnPhe: 1.868 ± 0.477
2.112GlnGly: 2.112 ± 0.444
0.65GlnHis: 0.65 ± 0.213
2.031GlnIle: 2.031 ± 0.37
4.386GlnLys: 4.386 ± 0.74
3.493GlnLeu: 3.493 ± 0.56
1.381GlnMet: 1.381 ± 0.358
2.031GlnAsn: 2.031 ± 0.435
1.706GlnPro: 1.706 ± 0.313
2.843GlnGln: 2.843 ± 0.7
1.787GlnArg: 1.787 ± 0.401
2.355GlnSer: 2.355 ± 0.37
1.624GlnThr: 1.624 ± 0.386
3.168GlnVal: 3.168 ± 0.435
0.162GlnTrp: 0.162 ± 0.093
1.624GlnTyr: 1.624 ± 0.371
0.0GlnXaa: 0.0 ± 0.0
Arg
2.68ArgAla: 2.68 ± 0.449
0.487ArgCys: 0.487 ± 0.18
3.33ArgAsp: 3.33 ± 0.482
4.467ArgGlu: 4.467 ± 0.537
2.193ArgPhe: 2.193 ± 0.436
2.843ArgGly: 2.843 ± 0.587
0.569ArgHis: 0.569 ± 0.212
3.33ArgIle: 3.33 ± 0.523
3.899ArgLys: 3.899 ± 0.631
4.63ArgLeu: 4.63 ± 0.633
0.812ArgMet: 0.812 ± 0.209
2.437ArgAsn: 2.437 ± 0.549
0.893ArgPro: 0.893 ± 0.293
1.706ArgGln: 1.706 ± 0.548
1.624ArgArg: 1.624 ± 0.381
2.762ArgSer: 2.762 ± 0.744
2.437ArgThr: 2.437 ± 0.448
2.437ArgVal: 2.437 ± 0.406
0.569ArgTrp: 0.569 ± 0.186
2.112ArgTyr: 2.112 ± 0.391
0.0ArgXaa: 0.0 ± 0.0
Ser
3.249SerAla: 3.249 ± 0.812
0.406SerCys: 0.406 ± 0.173
3.493SerAsp: 3.493 ± 0.569
3.411SerGlu: 3.411 ± 0.536
2.599SerPhe: 2.599 ± 0.54
3.33SerGly: 3.33 ± 0.629
1.381SerHis: 1.381 ± 0.341
3.086SerIle: 3.086 ± 0.503
6.173SerLys: 6.173 ± 0.697
5.929SerLeu: 5.929 ± 0.71
1.462SerMet: 1.462 ± 0.372
3.817SerAsn: 3.817 ± 0.552
1.3SerPro: 1.3 ± 0.281
2.031SerGln: 2.031 ± 0.411
2.112SerArg: 2.112 ± 0.413
2.762SerSer: 2.762 ± 0.431
3.817SerThr: 3.817 ± 0.663
4.142SerVal: 4.142 ± 0.498
0.65SerTrp: 0.65 ± 0.215
1.949SerTyr: 1.949 ± 0.361
0.0SerXaa: 0.0 ± 0.0
Thr
3.411ThrAla: 3.411 ± 0.844
0.244ThrCys: 0.244 ± 0.128
3.493ThrAsp: 3.493 ± 0.469
4.873ThrGlu: 4.873 ± 0.82
2.437ThrPhe: 2.437 ± 0.427
3.005ThrGly: 3.005 ± 0.566
0.487ThrHis: 0.487 ± 0.202
4.305ThrIle: 4.305 ± 0.441
4.873ThrLys: 4.873 ± 0.65
5.198ThrLeu: 5.198 ± 0.721
1.3ThrMet: 1.3 ± 0.291
2.112ThrAsn: 2.112 ± 0.434
1.787ThrPro: 1.787 ± 0.399
2.355ThrGln: 2.355 ± 0.359
2.112ThrArg: 2.112 ± 0.316
3.411ThrSer: 3.411 ± 0.381
3.817ThrThr: 3.817 ± 0.526
4.142ThrVal: 4.142 ± 0.653
0.406ThrTrp: 0.406 ± 0.181
2.112ThrTyr: 2.112 ± 0.337
0.0ThrXaa: 0.0 ± 0.0
Val
3.899ValAla: 3.899 ± 0.536
0.325ValCys: 0.325 ± 0.186
4.386ValAsp: 4.386 ± 0.57
5.929ValGlu: 5.929 ± 0.733
2.274ValPhe: 2.274 ± 0.426
3.899ValGly: 3.899 ± 0.626
0.975ValHis: 0.975 ± 0.314
3.736ValIle: 3.736 ± 0.545
4.955ValLys: 4.955 ± 0.517
4.792ValLeu: 4.792 ± 0.745
1.056ValMet: 1.056 ± 0.332
3.817ValAsn: 3.817 ± 0.583
1.949ValPro: 1.949 ± 0.375
2.518ValGln: 2.518 ± 0.553
3.411ValArg: 3.411 ± 0.507
4.467ValSer: 4.467 ± 0.494
3.086ValThr: 3.086 ± 0.353
4.548ValVal: 4.548 ± 0.526
0.812ValTrp: 0.812 ± 0.243
2.924ValTyr: 2.924 ± 0.585
0.0ValXaa: 0.0 ± 0.0
Trp
0.406TrpAla: 0.406 ± 0.263
0.244TrpCys: 0.244 ± 0.133
0.731TrpAsp: 0.731 ± 0.211
0.975TrpGlu: 0.975 ± 0.277
0.569TrpPhe: 0.569 ± 0.218
0.569TrpGly: 0.569 ± 0.177
0.244TrpHis: 0.244 ± 0.148
0.812TrpIle: 0.812 ± 0.366
0.893TrpLys: 0.893 ± 0.241
1.218TrpLeu: 1.218 ± 0.33
0.162TrpMet: 0.162 ± 0.116
0.65TrpAsn: 0.65 ± 0.219
0.081TrpPro: 0.081 ± 0.095
0.325TrpGln: 0.325 ± 0.16
0.569TrpArg: 0.569 ± 0.219
1.056TrpSer: 1.056 ± 0.344
0.731TrpThr: 0.731 ± 0.207
0.406TrpVal: 0.406 ± 0.203
0.162TrpTrp: 0.162 ± 0.139
0.406TrpTyr: 0.406 ± 0.244
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.68TyrAla: 2.68 ± 0.44
0.812TyrCys: 0.812 ± 0.27
1.949TyrAsp: 1.949 ± 0.453
2.68TyrGlu: 2.68 ± 0.361
2.193TyrPhe: 2.193 ± 0.459
2.031TyrGly: 2.031 ± 0.406
0.65TyrHis: 0.65 ± 0.201
2.437TyrIle: 2.437 ± 0.521
4.061TyrLys: 4.061 ± 0.625
3.249TyrLeu: 3.249 ± 0.615
0.487TyrMet: 0.487 ± 0.166
2.843TyrAsn: 2.843 ± 0.608
0.812TyrPro: 0.812 ± 0.312
1.137TyrGln: 1.137 ± 0.339
1.706TyrArg: 1.706 ± 0.403
1.949TyrSer: 1.949 ± 0.291
2.112TyrThr: 2.112 ± 0.352
2.924TyrVal: 2.924 ± 0.4
0.487TyrTrp: 0.487 ± 0.206
0.893TyrTyr: 0.893 ± 0.348
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (12313 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski