bacterium HR21

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1558 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5Y2I5|A0A2H5Y2I5_9BACT Thiol-disulfide oxidoreductase ResA OS=bacterium HR21 OX=2035416 GN=resA_5 PE=4 SV=1
MM1 pKa = 7.33FWTPEE6 pKa = 3.57LAAWLEE12 pKa = 4.16DD13 pKa = 4.07APWPATKK20 pKa = 10.09EE21 pKa = 4.02VLLDD25 pKa = 3.38YY26 pKa = 10.94AEE28 pKa = 4.71RR29 pKa = 11.84SGAPLQVIEE38 pKa = 4.59NLKK41 pKa = 10.11EE42 pKa = 4.05LPDD45 pKa = 5.25DD46 pKa = 3.73DD47 pKa = 4.5TEE49 pKa = 4.68YY50 pKa = 11.29EE51 pKa = 4.49GIEE54 pKa = 4.58DD55 pKa = 4.63IWPEE59 pKa = 3.57WADD62 pKa = 3.65FQLPDD67 pKa = 3.92YY68 pKa = 10.83LDD70 pKa = 3.86EE71 pKa = 6.18DD72 pKa = 3.92EE73 pKa = 5.49EE74 pKa = 4.33EE75 pKa = 4.29QEE77 pKa = 4.44EE78 pKa = 4.78YY79 pKa = 11.37

Molecular weight: 9.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5Y266|A0A2H5Y266_9BACT Uncharacterized protein OS=bacterium HR21 OX=2035416 GN=HRbin21_01341 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.7RR3 pKa = 11.84SSNAISRR10 pKa = 11.84RR11 pKa = 11.84PMPGLCRR18 pKa = 11.84HH19 pKa = 5.45SCYY22 pKa = 10.43LGLLIFLAGIVPLWGWCSRR41 pKa = 11.84EE42 pKa = 4.11DD43 pKa = 3.36STAALSALRR52 pKa = 11.84ARR54 pKa = 11.84VQALVEE60 pKa = 3.93QLQQRR65 pKa = 11.84ARR67 pKa = 11.84VGVLVSDD74 pKa = 3.92VAGRR78 pKa = 11.84RR79 pKa = 11.84YY80 pKa = 9.75FEE82 pKa = 5.09HH83 pKa = 6.97GAEE86 pKa = 4.0QLLVPASVTKK96 pKa = 10.21LFWSVAALAVLGDD109 pKa = 3.79SAVIRR114 pKa = 11.84TALYY118 pKa = 10.89ADD120 pKa = 4.33ALPRR124 pKa = 11.84ADD126 pKa = 3.7GTLSGNLYY134 pKa = 10.68LVGGGDD140 pKa = 3.81ALLTVQEE147 pKa = 4.52LEE149 pKa = 4.03EE150 pKa = 4.25LAAQLVRR157 pKa = 11.84RR158 pKa = 11.84GVRR161 pKa = 11.84RR162 pKa = 11.84ITGAVLGDD170 pKa = 3.33GTLFDD175 pKa = 4.13GQRR178 pKa = 11.84TRR180 pKa = 11.84LQYY183 pKa = 11.04SGDD186 pKa = 3.79SDD188 pKa = 3.99PVEE191 pKa = 4.31PLPPITALGFNRR203 pKa = 11.84NEE205 pKa = 3.62VRR207 pKa = 11.84VLVSATGRR215 pKa = 11.84RR216 pKa = 11.84VTAQTIPVSPGFVVDD231 pKa = 3.58VSGVRR236 pKa = 11.84IRR238 pKa = 11.84KK239 pKa = 6.88GARR242 pKa = 11.84RR243 pKa = 11.84GRR245 pKa = 11.84QRR247 pKa = 11.84VVLSARR253 pKa = 11.84SVMRR257 pKa = 11.84RR258 pKa = 11.84GVQYY262 pKa = 10.37IVVSGWIAARR272 pKa = 11.84GTWSLGVPMEE282 pKa = 4.33KK283 pKa = 10.28PEE285 pKa = 4.0VAAAATLVQRR295 pKa = 11.84LRR297 pKa = 11.84AAGIRR302 pKa = 11.84VEE304 pKa = 4.35GGFGEE309 pKa = 5.22GKK311 pKa = 9.98CPPGATLLGLWEE323 pKa = 4.55RR324 pKa = 11.84PLAEE328 pKa = 5.01LLAAVNKK335 pKa = 10.48EE336 pKa = 3.5SDD338 pKa = 3.57NFVAEE343 pKa = 4.52HH344 pKa = 6.59LFKK347 pKa = 11.32LLGARR352 pKa = 11.84AAGSGTHH359 pKa = 6.58AEE361 pKa = 4.06RR362 pKa = 11.84ARR364 pKa = 11.84RR365 pKa = 11.84LLQALLSRR373 pKa = 11.84WGIPCQGCALWDD385 pKa = 3.76GSGLSRR391 pKa = 11.84RR392 pKa = 11.84NRR394 pKa = 11.84VSAADD399 pKa = 3.49VVGMLRR405 pKa = 11.84AVFQLPIADD414 pKa = 3.92ALCRR418 pKa = 11.84SLSIAGVDD426 pKa = 3.54GTLKK430 pKa = 10.28QRR432 pKa = 11.84LQQTQAQARR441 pKa = 11.84VWAKK445 pKa = 10.11TGTLRR450 pKa = 11.84NASALAGYY458 pKa = 10.25VLTADD463 pKa = 4.04GDD465 pKa = 4.23PLVFAILSFGVVSRR479 pKa = 11.84AKK481 pKa = 10.46ALEE484 pKa = 4.4DD485 pKa = 3.62SLVATLARR493 pKa = 11.84FSFCGAGTQSRR504 pKa = 3.91

Molecular weight: 53.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1558

0

1558

581441

29

2214

373.2

41.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.005 ± 0.069

1.016 ± 0.019

3.84 ± 0.039

6.909 ± 0.075

3.747 ± 0.037

7.686 ± 0.069

2.211 ± 0.03

4.286 ± 0.043

1.818 ± 0.037

12.464 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.541 ± 0.023

2.012 ± 0.036

6.08 ± 0.05

4.396 ± 0.047

8.913 ± 0.062

5.115 ± 0.046

5.129 ± 0.052

8.012 ± 0.061

1.89 ± 0.03

2.929 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski